MymaA.00950.a

Lipoprotein aminopeptidase LpqL

CENTER ID: MymaA.00950.a
ORGANISM: Mycobacterium marinum ATCC BAA-535 / M
ASSOCIATED DISEASE: Cutaneous ulcerations, deep abscesses
CURRENT STATUS: expressed
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MymaA.00950.a.A2.GE30087 Mature protein( MymaA.00950.a ) 26 503
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|216594.6.peg.776
RefSeq: YP_001849040.1
UniProt: B2HQ08

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MVKKSRTMPA MVAVAVVAFL VVGCVRWSTQ TQTQPQPATS NPAAAEFAEA LRNRVTTEAM MGHLTKLQEI ADANNGTRAV GTPGYEASVD YVVNILRNSG FDVQTPEFSA RVFHSEKGSV DVGGMTAEAH ALEYSLGTPP DGVTGPLLLA PSDDSPGCTP SDYDNLPVKG AVVLVDRGEC QFAQKEDAAA QRGAVALIIA DNVDEQMMGG TLGVNTDVKI PVVSVTKSVG LQLRGKSGPA TIKLTASSQS FKARNVIAQT KTGSTSDVVM AGAHLDSVPQ GPGINDNGSG VAAVLETAVQ LGNSPHVHNA VRVAFWGAEE LGLIGSRNYV QSLDLDELKN IALYLNFDML ASPNPGYFTY DGDQSLPLDE RGQPVVPEGS AGIERTLVSY LRLSGKIAQD TSFDGRSDYD GFTLAGIPSG GLFSGAEVKM TEEQAKLWGG TADEPFDPNY HQKSDTIDHI DRTSLGIQGG GVAYAVGLYA QDLTGRNGVP VIGDRTRHVL AKP
NT Sequence
ATGGTGAAAA AATCACGGAC CATGCCGGCG ATGGTTGCCG TGGCCGTTGT TGCTTTCCTT GTGGTGGGTT GCGTTCGCTG GTCGACGCAG ACGCAGACTC AACCGCAGCC TGCTACCAGC AACCCTGCCG CAGCCGAGTT CGCCGAGGCG TTACGCAATC GGGTGACCAC CGAGGCCATG ATGGGGCACC TGACCAAGTT GCAGGAGATC GCCGACGCGA ACAACGGCAC GCGGGCGGTG GGCACGCCTG GCTATGAGGC CAGTGTCGAC TATGTGGTCA ACATCCTGCG CAACAGTGGT TTTGACGTGC AGACGCCCGA GTTTTCGGCC CGGGTGTTCC ATTCCGAAAA GGGATCGGTG GACGTCGGCG GGATGACCGC GGAGGCGCAC GCGCTGGAGT ACAGTCTCGG CACCCCGCCG GACGGGGTAA CCGGGCCGTT GCTGCTCGCG CCGTCGGATG ACAGCCCGGG TTGCACTCCC TCCGACTACG ACAACCTGCC GGTCAAAGGC GCGGTGGTAC TGGTGGACCG CGGCGAGTGT CAGTTCGCTC AGAAAGAAGA CGCCGCGGCG CAGCGCGGCG CCGTTGCACT GATCATCGCC GACAATGTCG ACGAGCAGAT GATGGGCGGC ACCTTGGGGG TGAACACCGA CGTCAAGATC CCGGTAGTGA GTGTCACCAA GTCGGTGGGG CTGCAGCTGC GCGGCAAGTC CGGGCCCGCG ACGATCAAGC TCACGGCGAG CAGTCAGAGC TTCAAGGCCC GCAACGTCAT TGCTCAGACC AAGACGGGGT CTACCAGCGA TGTCGTGATG GCGGGTGCGC ATCTGGACAG CGTGCCGCAG GGGCCAGGCA TCAACGACAA CGGTTCGGGC GTGGCTGCGG TATTGGAAAC CGCGGTACAG CTGGGTAATT CGCCACACGT GCACAACGCT GTTCGCGTTG CGTTCTGGGG CGCCGAGGAG CTGGGCCTGA TTGGATCGCG CAACTACGTC CAGTCCCTGG ACCTCGATGA GCTCAAGAAC ATTGCGCTGT ATCTGAACTT CGACATGCTG GCCTCGCCCA ATCCCGGATA TTTCACCTAT GACGGTGATC AGTCGCTACC GCTGGATGAG CGCGGCCAGC CGGTGGTGCC CGAAGGTTCG GCGGGAATCG AGCGCACTCT GGTCTCCTAT CTCAGGTTGT CCGGCAAGAT CGCGCAGGAC ACCTCGTTTG ACGGTCGTTC CGACTACGAC GGATTCACCT TGGCGGGGAT CCCGTCGGGC GGCCTGTTTT CCGGTGCTGA GGTCAAAATG ACCGAGGAAC AGGCAAAGCT GTGGGGCGGC ACCGCCGATG AGCCCTTCGA TCCCAACTAT CACCAGAAAT CCGACACCAT CGACCACATC GACCGCACGT CGCTGGGCAT CCAGGGAGGC GGCGTGGCCT ACGCCGTGGG TTTGTACGCG CAAGACCTGA CCGGCCGAAA CGGCGTTCCA GTCATCGGGG ACCGTACCCG CCACGTGCTC GCCAAACCA
Details for MymaA.00950.a.A2.GE30087
HARVESTED ON: 8/10/2010
SEQUENCED ON: 8/16/2010
EXPECTED MW: 52kDa
OBSERVED MW: 64kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 95
PERCENT COVERAGE: 63
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMRWSTQTQT QPQPATSNPA AAEFAEALRN RVTTEAMMGH LTKLQEIADA NNGTRAVGTP GYEASVDYVV NILRNSGFDV QTPEFSARVF HSEKGSVDVG GMTAEAHALE YSLGTPPDGV TGPLLLAPSD DSPGCTPSDY DNLPVKGAVV LVDRGECQFA QKEDAAAQRG AVALIIADNV DEQMMGGTLG VNTDVKIPVV SVTKSVGLQL RGKSGPATIK LTASSQSFKA RNVIAQTKTG STSDVVMAGA HLDSVPQGXX IXDNGSGVXA XLETAXQLGN SPHVXNXXRV AFWGXXXWX
Validated NT Sequence
ttttgtttan ctttaagaag gagatatacc atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgcgct ggtcgacgca gacgcagact caaccgcagc ctgctaccag caaccctgcc gcagccgagt tcgccgaggc gttacgcaat cgggtgacca ccgaggccat gatggggcac ctgaccaagt tgcaggagat cgccgacgcg aacaacggca cgcgggcggt gggcacgcct ggctatgagg ccagtgtcga ctatgtggtc aacatcctgc gcaacagtgg ttttgacgtg cagacgcccg agttttcggc ccgggtgttc cattccgaaa agggatcggt ggacgtcggc gggatgaccg cggaggcgca cgcgctggag tacagtctcg gcaccccgcc ggacggggta accgggccgt tgctgctcgc gccgtcggat gacagcccgg gttgcactcc ctccgactac gacaacctgc cggtcaaagg cgcggtggta ctggtggacc gcggcgagtg tcagttcgct cagaaagaag acgccgcggc gcagcgcggc gccgttgcac tgatcatcgc cgacaatgtc gacgagcaga tgatgggcgg caccttgggg gtgaacaccg acgtcaagat cccggtagtg agtgtcacca agtcggtggg gctgcagctg cgcggcaagt ccgggcccgc gacgatcaag ctcacggcga gcagtcagag cttcaaggcc cgcaacgtca ttgctcagac caagacgggg tctaccagcg atgtcgtgat ggcgggngcg catctggaca gcgtgccnca ggggncangc atcnacgaca acggntcggg cgtggntgcg gnnttggaaa ccgcnntaca gctgggtaat tcnccacacg tgcncaacgn tnntcgcgtt gcgttctggg gcgncnannn ctgggnntga ntggntcgcg caactacgtc nantncctgn nnnnnatnan ctcnnnnncn nnngctnnnt ctgancnncn acatgctngn ntcgnccnan nnnnnnnnnn nnncnnannn nggnnannnn nnnnnnccan nnnnnnnnnn cnnnnnnnnn cnnnnnnnnn ggnnnnnnnn nnnnnnnnnn nnnnngnnan nnnnnnnnnn nnnnnct
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMRWSTQTQT QPQPATSNPA AAEFAEALRN RVTTEAMMGH LTKLQEIADA NNGTRAVGTP GYEASVDYVV NILRNSGFDV QTPEFSARVF HSEKGSVDVG GMTAEAHALE YSLGTPPDGV TGPLLLAPSD DSPGCTPSDY DNLPVKGAVV LVDRGECQFA QKEDAAAQRG AVALIIADNV DEQMMGGTLG VNTDVKIPVV SVTKSVGLQL RGKSGPATIK LTASSQSFKA RNVIAQTKTG STSDVVMAGA HLDSVPQGPG INDNGSGVAA VLETAVQLGN SPHVHNAVRV AFWGAEELGL IGSRNYVQSL DLDELKNIAL YLNFDMLASP NPGYFTYDGD QSLPLDERGQ PVVPEGSAGI ERTLVSYLRL SGKIAQDTSF DGRSDYDGFT LAGIPSGGLF SGAEVKMTEE QAKLWGGTAD EPFDPNYHQK SDTIDHIDRT SLGIQGGGVA YAVGLYAQDL TGRNGVPVIG DRTRHVLAK
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gcgctggtcg acgcagacgc agactcaacc gcagcctgct accagcaacc ctgccgcagc cgagttcgcc gaggcgttac gcaatcgggt gaccaccgag gccatgatgg ggcacctgac caagttgcag gagatcgccg acgcgaacaa cggcacgcgg gcggtgggca cgcctggcta tgaggccagt gtcgactatg tggtcaacat cctgcgcaac agtggttttg acgtgcagac gcccgagttt tcggcccggg tgttccattc cgaaaaggga tcggtggacg tcggcgggat gaccgcggag gcgcacgcgc tggagtacag tctcggcacc ccgccggacg gggtaaccgg gccgttgctg ctcgcgccgt cggatgacag cccgggttgc actccctccg actacgacaa cctgccggtc aaaggcgcgg tggtactggt ggaccgcggc gagtgtcagt tcgctcagaa agaagacgcc gcggcgcagc gcggcgccgt tgcactgatc atcgccgaca atgtcgacga gcagatgatg ggcggcacct tgggggtgaa caccgacgtc aagatcccgg tagtgagtgt caccaagtcg gtggggctgc agctgcgcgg caagtccggg cccgcgacga tcaagctcac ggcgagcagt cagagcttca aggcccgcaa cgtcattgct cagaccaaga cggggtctac cagcgatgtc gtgatggcgg gtgcgcatct ggacagcgtg ccgcaggggc caggcatcaa cgacaacggt tcgggcgtgg ctgcggtatt ggaaaccgcg gtacagctgg gtaattcgcc acacgtgcac aacgctgttc gcgttgcgtt ctggggcgcc gaggagctgg gcctgattgg atcgcgcaac tacgtccagt ccctggacct cgatgagctc aagaacattg cgctgtatct gaacttcgac atgctggcct cgcccaatcc cggatatttc acctatgacg gtgatcagtc gctaccgctg gatgagcgcg gccagccggt ggtgcccgaa ggttcggcgg gaatcgagcg cactctggtc tcctatctca ggttgtccgg caagatcgcg caggacacct cgtttgacgg tcgttccgac tacgacggat tcaccttggc ggggatcccg tcgggcggcc tgttttccgg tgctgaggtc aaaatgaccg aggaacaggc aaagctgtgg ggcggcaccg ccgatgagcc cttcgatccc aactatcacc agaaatccga caccatcgac cacatcgacc gcacgtcgct gggcatccag ggaggcggcg tggcctacgc cgtgggtttg tacgcgcaag acctgaccgg ccgaaacggc gttccagtca tcggggaccg tacccgccac gtgctcgcca aaaaacagca cgaacaagtt ctgcagccaa gcttctcgag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaa