MymaA.00896.b

Citrate synthase II CitA

CENTER ID: MymaA.00896.b
ORGANISM: Mycobacterium marinum ATCC BAA-535 / M
ASSOCIATED DISEASE: Cutaneous ulcerations, deep abscesses
CURRENT STATUS: purified
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MymaA.00896.b.A1.GE30083 Full length( MymaA.00896.b ) 1 373
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MymaA.00896.b.A1.PS00872 Full length( MymaA.00896.b ) 1 373

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|216594.6.peg.4988
RefSeq: YP_001852901.1
UniProt: B2HF84

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTVVPEDFVP GLNGVVAFTT EIAEPDKDGG ALRYRGVDIE DLVSLRVTFG DVWALLVDGE FGRGLPPAEP FPLPIHTGDV RVDVQAGLAM LAPIWGYAPL LDIDDATARE QLARASVMAL SYVAQSARGI YQPAVPQRVI DECSTVTARF MTRWQGEPDP RHIEAIDAYW VSAAEHGMNA STFTARVIAS TGADVAAALS GAIGAMSGPL HGGAPARVLP MIEQVERTGD ARGLVKGILD RGEKLMGFGH RVYRAEDPRA RVLRATAERL AAPRYEVAAA LEQAALAELR ERRPDRAIET NVEFWAAVIL DFAGVPANMM PAMFTCGRTA GWCAHILEQK RLGKLVRPSA IYVGPAPRTP ESVAGWDRVL TTA
NT Sequence
ATGACTGTGG TCCCGGAAGA TTTTGTCCCT GGCCTCAATG GCGTAGTCGC CTTCACCACC GAGATCGCGG AGCCGGATAA AGACGGTGGC GCGCTGCGCT ATCGCGGCGT CGATATCGAA GATCTCGTTA GTCTGCGGGT GACATTCGGC GATGTGTGGG CGCTGCTGGT CGACGGGGAA TTCGGCCGCG GGCTGCCGCC CGCCGAGCCA TTCCCGCTGC CGATCCACAC CGGCGATGTA CGAGTCGACG TTCAGGCTGG GCTGGCGATG CTGGCGCCCA TCTGGGGCTA CGCCCCACTG CTGGACATCG ATGACGCCAC GGCCCGCGAA CAGTTGGCGC GTGCGTCGGT GATGGCGCTG TCGTACGTCG CTCAATCAGC ACGCGGCATC TATCAGCCGG CCGTGCCTCA GCGAGTCATC GACGAATGCT CAACGGTCAC AGCACGTTTC ATGACGCGAT GGCAAGGTGA ACCGGACCCC AGGCACATCG AAGCCATTGA CGCCTACTGG GTGTCGGCCG CCGAACACGG CATGAACGCC TCGACCTTCA CCGCCCGCGT CATCGCCTCT ACCGGCGCGG ACGTCGCAGC GGCACTCTCG GGCGCGATCG GTGCGATGAG CGGGCCGCTG CACGGCGGTG CACCGGCTCG AGTCCTCCCC ATGATCGAGC AGGTCGAGCG CACCGGAGAC GCCCGCGGCC TGGTCAAGGG CATTCTGGAC CGCGGCGAGA AGCTGATGGG CTTCGGGCAT CGGGTCTACC GCGCGGAGGA CCCGCGGGCG CGGGTGCTGC GCGCCACCGC CGAGCGGCTG GCGGCACCCC GGTACGAAGT CGCGGCGGCC TTGGAACAGG CGGCCCTGGC CGAGTTGCGT GAGCGCCGTC CCGACCGAGC CATCGAGACC AATGTCGAGT TCTGGGCCGC GGTCATTCTG GACTTCGCCG GCGTCCCGGC CAACATGATG CCGGCGATGT TCACCTGCGG GCGGACCGCC GGCTGGTGTG CCCACATTCT CGAGCAGAAG CGGCTGGGCA AGCTGGTCCG CCCCTCCGCG ATCTACGTGG GGCCGGCGCC GCGCACCCCG GAGTCGGTCG CGGGCTGGGA CCGGGTGCTG ACCACCGCA
Details for MymaA.00896.b.A1.GE30083
HARVESTED ON: 8/10/2010
SEQUENCED ON: 8/16/2010
EXPECTED MW: 42kDa
OBSERVED MW: 42kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 100
PERCENT COVERAGE: 84
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMTVVPEDFV PGLNGVVAFT TEIAEPDKDG GALRYRGVDI EDLVSLRVTF GDVWALLVDG EFGRGLPPAE PFPLPIHTGD VRVDVQAGLA MLAPIWGYAP LLDIDDATAR EQLARASVMA LSYVAQSARG IYQPAVPQRV IDECSTVTAR FMTRWQGEPD PRHIEAIDAY WVSAAEHGMN ASTFTARVIA STGADVAAAL SGAIGAMSGP LHGGAPARVL PMIEQVERTG DARGLVKGIL DRGEKLMGFG HRVYRAEDPR ARVLRATAER LAAPRYEVAA ALEQAALAEL RERRPDRAIE TNVEFWAAVI LD
Validated NT Sequence
aagtccagaa tgaccgcggc ccagaactcg acattggtct cgatggctcg gtcgggacgg cgctcacgca actcggccag ggccgcctgt tccaaggccg ccgcgacttc gtaccggggt gccgccagcc gctcggcggt ggcgcgcagc acccgcgccc gcgggtcctc cgcgcggtag acccgatgcc cgaagcccat cagcttctcg ccgcggtcca gaatgccctt gaccaggccg cgggcgtctc cggtgcgctc gacctgctcg atcatgggga ggactcgagc cggtgcaccg ccgtgcagcg gcccgctcat cgcaccgatc gcgcccgaga gtgccgctgc gacgtccgcg ccggtagagg cgatgacgcg ggcggtgaag gtcgaggcgt tcatgccgtg ttcggcggcc gacacccagt aggcgtcaat ggcttcgatg tgcctggggt ccggttcacc ttgccatcgc gtcatgaaac gtgctgtgac cgttgagcat tcgtcgatga ctcgctgagg cacggccggc tgatagatgc cgcgtgctga ttgagcgacg tacgacagcg ccatcaccga cgcacgcgcc aactgttcgc gggccgtggc gtcatcgatg tccagcagtg gggcgtagcc ccagatgggc gccagcatcg ccagcccagc ctgaacgtcg actcgtacat cgccggtgtg gatcggcagc gggaatggct cggcgggcgg cagcccgcgg ccgaattccc cgtcgaccag cagcgcccac acatcgccga atgtcacccg cagactaacg agatcttcga tatcgacgcc gcgatagcgc agcgcgccac cgtctttatc cggctccgcg atctcggtgg tgaaggcgac tacgccattg aggccaggga caaaatcttc cgggaccaca gtcatcgaac caggaccctg ggtctgagct tccagggtac ccatatgatg gtgatggtga tgagccat
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMTVVPEDFV PGLNGVVAFT TEIAEPDKDG GALRYRGVDI EDLVSLRVTF GDVWALLVDG EFGRGLPPAE PFPLPIHTGD VRVDVQAGLA MLAPIWGYAP LLDIDDATAR EQLARASVMA LSYVAQSARG IYQPAVPQRV IDECSTVTAR FMTRWQGEPD PRHIEAIDAY WVSAAEHGMN ASTFTARVIA STGADVAAAL SGAIGAMSGP LHGGAPARVL PMIEQVERTG DARGLVKGIL DRGEKLMGFG HRVYRAEDPR ARVLRATAER LAAPRYEVAA ALEQAALAEL RERRPDRAIE TNVEFWAAVI LDFAGVPANM MPAMFTCGRT AGWCAHILEQ KRLGKLVRPS AIYVGPAPRT PESVAGWDRV LTT
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gactgtggtc ccggaagatt ttgtccctgg cctcaatggc gtagtcgcct tcaccaccga gatcgcggag ccggataaag acggtggcgc gctgcgctat cgcggcgtcg atatcgaaga tctcgttagt ctgcgggtga cattcggcga tgtgtgggcg ctgctggtcg acggggaatt cggccgcggg ctgccgcccg ccgagccatt cccgctgccg atccacaccg gcgatgtacg agtcgacgtt caggctgggc tggcgatgct ggcgcccatc tggggctacg ccccactgct ggacatcgat gacgccacgg cccgcgaaca gttggcgcgt gcgtcggtga tggcgctgtc gtacgtcgct caatcagcac gcggcatcta tcagccggcc gtgcctcagc gagtcatcga cgaatgctca acggtcacag cacgtttcat gacgcgatgg caaggtgaac cggaccccag gcacatcgaa gccattgacg cctactgggt gtcggccgcc gaacacggca tgaacgcctc gaccttcacc gcccgcgtca tcgcctctac cggcgcggac gtcgcagcgg cactctcggg cgcgatcggt gcgatgagcg ggccgctgca cggcggtgca ccggctcgag tcctccccat gatcgagcag gtcgagcgca ccggagacgc ccgcggcctg gtcaagggca ttctggaccg cggcgagaag ctgatgggct tcgggcatcg ggtctaccgc gcggaggacc cgcgggcgcg ggtgctgcgc gccaccgccg agcggctggc ggcaccccgg tacgaagtcg cggcggcctt ggaacaggcg gccctggccg agttgcgtga gcgccgtccc gaccgagcca tcgagaccaa tgtcgagttc tgggccgcgg tcattctgga cttcgccggc gtcccggcca acatgatgcc ggcgatgttc acctgcgggc ggaccgccgg ctggtgtgcc cacattctcg agcagaagcg gctgggcaag ctggtccgcc cctccgcgat ctacgtgggg ccggcgccgc gcaccccgga gtcggtcgcg ggctgggacc gggtgctgac caccaaacag cacgaacaag ttctgcagcc aagcttctcg aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaa
Details for MymaA.00896.b.A1.PS00872
PURIFICATION DATe: 11/19/2010
CONCENTRATION: 52.03mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 10
VIAL VOLUME: 200µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 84
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMTVVPEDFV PGLNGVVAFT TEIAEPDKDG GALRYRGVDI EDLVSLRVTF GDVWALLVDG EFGRGLPPAE PFPLPIHTGD VRVDVQAGLA MLAPIWGYAP LLDIDDATAR EQLARASVMA LSYVAQSARG IYQPAVPQRV IDECSTVTAR FMTRWQGEPD PRHIEAIDAY WVSAAEHGMN ASTFTARVIA STGADVAAAL SGAIGAMSGP LHGGAPARVL PMIEQVERTG DARGLVKGIL DRGEKLMGFG HRVYRAEDPR ARVLRATAER LAAPRYEVAA ALEQAALAEL RERRPDRAIE TNVEFWAAVI LD
Validated NT Sequence
aagtccagaa tgaccgcggc ccagaactcg acattggtct cgatggctcg gtcgggacgg cgctcacgca actcggccag ggccgcctgt tccaaggccg ccgcgacttc gtaccggggt gccgccagcc gctcggcggt ggcgcgcagc acccgcgccc gcgggtcctc cgcgcggtag acccgatgcc cgaagcccat cagcttctcg ccgcggtcca gaatgccctt gaccaggccg cgggcgtctc cggtgcgctc gacctgctcg atcatgggga ggactcgagc cggtgcaccg ccgtgcagcg gcccgctcat cgcaccgatc gcgcccgaga gtgccgctgc gacgtccgcg ccggtagagg cgatgacgcg ggcggtgaag gtcgaggcgt tcatgccgtg ttcggcggcc gacacccagt aggcgtcaat ggcttcgatg tgcctggggt ccggttcacc ttgccatcgc gtcatgaaac gtgctgtgac cgttgagcat tcgtcgatga ctcgctgagg cacggccggc tgatagatgc cgcgtgctga ttgagcgacg tacgacagcg ccatcaccga cgcacgcgcc aactgttcgc gggccgtggc gtcatcgatg tccagcagtg gggcgtagcc ccagatgggc gccagcatcg ccagcccagc ctgaacgtcg actcgtacat cgccggtgtg gatcggcagc gggaatggct cggcgggcgg cagcccgcgg ccgaattccc cgtcgaccag cagcgcccac acatcgccga atgtcacccg cagactaacg agatcttcga tatcgacgcc gcgatagcgc agcgcgccac cgtctttatc cggctccgcg atctcggtgg tgaaggcgac tacgccattg aggccaggga caaaatcttc cgggaccaca gtcatcgaac caggaccctg ggtctgagct tccagggtac ccatatgatg gtgatggtga tgagccat
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMTVVPEDFV PGLNGVVAFT TEIAEPDKDG GALRYRGVDI EDLVSLRVTF GDVWALLVDG EFGRGLPPAE PFPLPIHTGD VRVDVQAGLA MLAPIWGYAP LLDIDDATAR EQLARASVMA LSYVAQSARG IYQPAVPQRV IDECSTVTAR FMTRWQGEPD PRHIEAIDAY WVSAAEHGMN ASTFTARVIA STGADVAAAL SGAIGAMSGP LHGGAPARVL PMIEQVERTG DARGLVKGIL DRGEKLMGFG HRVYRAEDPR ARVLRATAER LAAPRYEVAA ALEQAALAEL RERRPDRAIE TNVEFWAAVI LDFAGVPANM MPAMFTCGRT AGWCAHILEQ KRLGKLVRPS AIYVGPAPRT PESVAGWDRV LTT
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GACTGTGGTC CCGGAAGATT TTGTCCCTGG CCTCAATGGC GTAGTCGCCT TCACCACCGA GATCGCGGAG CCGGATAAAG ACGGTGGCGC GCTGCGCTAT CGCGGCGTCG ATATCGAAGA TCTCGTTAGT CTGCGGGTGA CATTCGGCGA TGTGTGGGCG CTGCTGGTCG ACGGGGAATT CGGCCGCGGG CTGCCGCCCG CCGAGCCATT CCCGCTGCCG ATCCACACCG GCGATGTACG AGTCGACGTT CAGGCTGGGC TGGCGATGCT GGCGCCCATC TGGGGCTACG CCCCACTGCT GGACATCGAT GACGCCACGG CCCGCGAACA GTTGGCGCGT GCGTCGGTGA TGGCGCTGTC GTACGTCGCT CAATCAGCAC GCGGCATCTA TCAGCCGGCC GTGCCTCAGC GAGTCATCGA CGAATGCTCA ACGGTCACAG CACGTTTCAT GACGCGATGG CAAGGTGAAC CGGACCCCAG GCACATCGAA GCCATTGACG CCTACTGGGT GTCGGCCGCC GAACACGGCA TGAACGCCTC GACCTTCACC GCCCGCGTCA TCGCCTCTAC CGGCGCGGAC GTCGCAGCGG CACTCTCGGG CGCGATCGGT GCGATGAGCG GGCCGCTGCA CGGCGGTGCA CCGGCTCGAG TCCTCCCCAT GATCGAGCAG GTCGAGCGCA CCGGAGACGC CCGCGGCCTG GTCAAGGGCA TTCTGGACCG CGGCGAGAAG CTGATGGGCT TCGGGCATCG GGTCTACCGC GCGGAGGACC CGCGGGCGCG GGTGCTGCGC GCCACCGCCG AGCGGCTGGC GGCACCCCGG TACGAAGTCG CGGCGGCCTT GGAACAGGCG GCCCTGGCCG AGTTGCGTGA GCGCCGTCCC GACCGAGCCA TCGAGACCAA TGTCGAGTTC TGGGCCGCGG TCATTCTGGA CTTCGCCGGC GTCCCGGCCA ACATGATGCC GGCGATGTTC ACCTGCGGGC GGACCGCCGG CTGGTGTGCC CACATTCTCG AGCAGAAGCG GCTGGGCAAG CTGGTCCGCC CCTCCGCGAT CTACGTGGGG CCGGCGCCGC GCACCCCGGA GTCGGTCGCG GGCTGGGACC GGGTGCTGAC CACCAAACAG CACGAACAAG TTCTGCAGCC AAGCTTCTCG AGGATCCGGC TGCTAACAAA GCCCGAAAGG AAGCTGAGTT GGCTGCTGCC ACCGCTGAGC AATAACTAGC ATAACCCCTT GGGGCCTCTA AACGGGTCTT GAGGGGTTTT TTGCTGAAAG GAGGAACTAT ATCCGGATAT CCACAGGACG GGTGTGGTCG CCATGATCGC GTAGTCGATA GTGGCTCCAA GTAGCGAAGC GAGCAGGACT GGGCGGCGGC CAAAGCGGTC GGACAGTGCT CCGAGAACGG GTGCGCATAG AAATTGCATC AACGCATATA GCGCTAGCAG CACGCCATAG TGACTGGCGA TGCTGTCGGA ATGGACGATA TCCCGCAAGA GGCCCGGCAG TACCGGCATA ACCAAGCCTA TGCCTACAGC ATCCAGGGTG ACGGTGCCGA GGATGACGAT GAGCGCATTG TTAGATTTCA TACACGGTGC CTGACTGCGT TAGCAATTTA ACTGTGATAA ACTACCGCAT TAAAGCTTAT CGATGATAAG CTGTCAAACA TGAGAA