MymaA.00896.a

Citrate synthase

CENTER ID: MymaA.00896.a
ORGANISM: Mycobacterium marinum ATCC BAA-535 / M
ASSOCIATED DISEASE: Cutaneous ulcerations, deep abscesses
CURRENT STATUS: diffraction
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MymaA.00896.a.A1.GE30082 Full length( MymaA.00896.a ) 1 431
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MymaA.00896.a.A1.PS00876 Full length( MymaA.00896.a ) 1 431

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|216594.6.peg.4985
RefSeq: YP_001852898.1
UniProt: B2HF81

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSDTDDTATL KYPGGEIDLQ IAHATEGADG IALGPLLSKT GHTTFDNGFV NTASCKSSIT YIDGDAGILR YRGYPIDQLA EKSTFIEVCY LLIYGELPNT DQLAQFTGRI QRHTMLHEDL KRFFDGFPRN AHPMPVLSSV VNALSAYYQD ALDPMDSEQV ELSTIRLLAK LPTIAAYAYK KSVGQPFLYP ENSLTLVENF LRMTFGFPAE PYHPDPEVVR ALDMLFILHA DHEQNCSTST VRLVGSSRAN LFTSISGGIN ALWGPLHGGA NQAVLEMLEQ IRDSGDDVSE FVRKVKNREA GVKLMGFGHR VYKNYDPRAR IVKEQADKIL SKLGGDDSLL GIAKELEEAA LTDDYFIERK LYPNVDFYTG LIYRALGFPT RMFTVLFALG RLPGWIAHWR EMHDEGDSKI GRPRQIYTGY TERDYVAMDG R
NT Sequence
GTGTCCGACA CCGACGACAC CGCAACTCTG AAGTACCCGG GGGGCGAGAT CGACCTGCAG ATCGCCCATG CCACCGAGGG TGCTGACGGC ATTGCGCTCG GCCCGCTGCT GTCCAAGACC GGACACACCA CGTTCGACAA CGGCTTCGTC AATACCGCGT CGTGCAAAAG CTCAATCACC TACATCGACG GTGACGCCGG CATTCTGCGC TACCGCGGAT ACCCGATCGA CCAGCTGGCG GAGAAGTCGA CCTTCATCGA GGTCTGCTAC CTGCTGATCT ACGGCGAGTT GCCCAACACC GACCAGCTCG CCCAGTTCAC CGGCCGGATC CAGCGCCACA CCATGCTGCA CGAGGACCTC AAGCGGTTCT TCGACGGTTT CCCTCGCAAC GCCCACCCGA TGCCAGTGCT GTCCAGCGTG GTCAACGCGC TCTCGGCGTA CTACCAGGAC GCGCTGGACC CGATGGACAG CGAGCAGGTT GAGCTCTCGA CGATCCGGTT GCTGGCCAAG CTGCCGACCA TCGCCGCCTA CGCCTACAAG AAGTCGGTTG GCCAGCCGTT CCTCTACCCG GAGAACTCCC TGACGCTGGT CGAGAACTTC TTGCGGATGA CGTTCGGCTT CCCCGCCGAG CCCTACCACC CCGACCCTGA GGTGGTGCGG GCGCTGGACA TGCTGTTCAT CCTGCACGCC GACCACGAGC AGAACTGCTC GACCTCCACG GTGCGGCTGG TGGGTTCCTC CCGGGCCAAC CTGTTCACCT CGATCTCGGG CGGCATCAAC GCCCTCTGGG GCCCGCTGCA CGGCGGTGCC AACCAGGCGG TGCTCGAGAT GCTCGAGCAG ATCCGCGACA GCGGCGACGA CGTGAGCGAG TTCGTCCGCA AGGTGAAGAA CCGCGAAGCC GGCGTCAAGC TGATGGGCTT TGGCCACCGC GTCTACAAGA ACTACGACCC CCGCGCCAGG ATCGTCAAGG AGCAGGCCGA CAAGATCCTG TCCAAGCTCG GCGGCGATGA CTCCCTGCTG GGTATCGCGA AGGAGCTCGA AGAGGCGGCG TTGACCGACG ACTACTTCAT CGAGCGCAAG CTCTACCCGA ACGTCGACTT CTACACCGGC CTGATCTACC GTGCCCTCGG CTTCCCCACC CGGATGTTCA CCGTGCTGTT TGCGCTGGGC CGGCTGCCGG GCTGGATCGC GCACTGGCGC GAAATGCACG ACGAGGGCGA CAGCAAGATC GGGCGTCCGC GCCAGATCTA CACCGGTTAC ACCGAGCGCG ACTACGTCGC GATGGACGGG CGG
Details for MymaA.00896.a.A1.GE30082
HARVESTED ON: 8/10/2010
SEQUENCED ON: 8/16/2010
EXPECTED MW: 50kDa
OBSERVED MW: 50kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 100
PERCENT COVERAGE: 81
Validated AA Sequence
MVSDTDDTAT LKYPGGEIDL QIAHATEGAD GIALGPLLSK TGHTTFDNGF VNTASCKSSI TYIDGDAGIL RYRGYPIDQL AEKSTFIEVC YLLIYGELPN TDQLAQFTGR IQRHTMLHED LKRFFDGFPR NAHPMPVLSS VVNALSAYYQ DALDPMDSEQ VELSTIRLLA KLPTIAAYAY KKSVGQPFLY PENSLTLVEN FLRMTFGFPA EPYHPDPEVV RALDMLFILH ADHEQNCSTS TVRLVGSSRA NLFTSISGGI NALWGPLHGG ANQAVLEMLE QIRDSGDDVS EFVRKVKNRE AGVKLMGFGH RVYKNYDPRA RIVKEQADKI LSKLGGDDSL LGIAKELEEA ALTDDYFIER KLYPNVDFYT
Validated NT Sequence
gncggtgtag aagtcgacgt tcgggtagag cttgcgctcg atgaagtagt cgtcggtcaa cgccgcctct tcgagctcct tcgcgatacc cagcagggag tcatcgccgc cgagcttgga caggatcttg tcggcctgct ccttgacgat cctggcgcgg gggtcgtagt tcttgtagac gcggtggcca aagcccatca gcttgacgcc ggcttcgcgg ttcttcacct tgcggacgaa ctcgctcacg tcgtcgccgc tgtcgcggat ctgctcgagc atctcgagca ccgcctggtt ggcaccgccg tgcagcgggc cccagagggc gttgatgccg cccgagatcg aggtgaacag gttggcccgg gaggaaccca ccagccgcac cgtggaggtc gagcagttct gctcgtggtc ggcgtgcagg atgaacagca tgtccagcgc ccgcaccacc tcagggtcgg ggtggtaggg ctcggcgggg aagccgaacg tcatccgcaa gaagttctcg accagcgtca gggagttctc cgggtagagg aacggctggc caaccgactt cttgtaggcg taggcggcga tggtcggcag cttggccagc aaccggatcg tcgagagctc aacctgctcg ctgtccatcg ggtccagcgc gtcctggtag tacgccgaga gcgcgttgac cacgctggac agcactggca tcgggtgggc gttgcgaggg aaaccgtcga agaaccgctt gaggtcctcg tgcagcatgg tgtggcgctg gatccggccg gtgaactggg cgagctggtc ggtgttgggc aactcgccgt agatcagcag gtagcagacc tcgatgaagg tcgacttctc cgccagctgg tcgatcgggt atccgcggta gcgcagaatg ccggcgtcac cgtcgatgta ggtgattgag cttttgcacg acgcggtatt gacgaagccg ttgtcgaacg tggtgtgtcc ggtcttggac agcagcgggc cgagcgcaat gccgtcagca ccctcggtgg catgggcgat ctgcaggtcg atctcgcccc ccgggtactt cagagttgcg gtgtcgtcgg tgtcggacac catcgaacca ggaccctggg tctgagcttc caggg
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVSDTDDTA TLKYPGGEID LQIAHATEGA DGIALGPLLS KTGHTTFDNG FVNTASCKSS ITYIDGDAGI LRYRGYPIDQ LAEKSTFIEV CYLLIYGELP NTDQLAQFTG RIQRHTMLHE DLKRFFDGFP RNAHPMPVLS SVVNALSAYY QDALDPMDSE QVELSTIRLL AKLPTIAAYA YKKSVGQPFL YPENSLTLVE NFLRMTFGFP AEPYHPDPEV VRALDMLFIL HADHEQNCST STVRLVGSSR ANLFTSISGG INALWGPLHG GANQAVLEML EQIRDSGDDV SEFVRKVKNR EAGVKLMGFG HRVYKNYDPR ARIVKEQADK ILSKLGGDDS LLGIAKELEE AALTDDYFIE RKLYPNVDFY TGLIYRALGF PTRMFTVLFA LGRLPGWIAH WREMHDEGDS KIGRPRQIYT GYTERDYVAM DG
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtgtccgac accgacgaca ccgcaactct gaagtacccg gggggcgaga tcgacctgca gatcgcccat gccaccgagg gtgctgacgg cattgcgctc ggcccgctgc tgtccaagac cggacacacc acgttcgaca acggcttcgt caataccgcg tcgtgcaaaa gctcaatcac ctacatcgac ggtgacgccg gcattctgcg ctaccgcgga tacccgatcg accagctggc ggagaagtcg accttcatcg aggtctgcta cctgctgatc tacggcgagt tgcccaacac cgaccagctc gcccagttca ccggccggat ccagcgccac accatgctgc acgaggacct caagcggttc ttcgacggtt tccctcgcaa cgcccacccg atgccagtgc tgtccagcgt ggtcaacgcg ctctcggcgt actaccagga cgcgctggac ccgatggaca gcgagcaggt tgagctctcg acgatccggt tgctggccaa gctgccgacc atcgccgcct acgcctacaa gaagtcggtt ggccagccgt tcctctaccc ggagaactcc ctgacgctgg tcgagaactt cttgcggatg acgttcggct tccccgccga gccctaccac cccgaccctg aggtggtgcg ggcgctggac atgctgttca tcctgcacgc cgaccacgag cagaactgct cgacctccac ggtgcggctg gtgggttcct cccgggccaa cctgttcacc tcgatctcgg gcggcatcaa cgccctctgg ggcccgctgc acggcggtgc caaccaggcg gtgctcgaga tgctcgagca gatccgcgac agcggcgacg acgtgagcga gttcgtccgc aaggtgaaga accgcgaagc cggcgtcaag ctgatgggct ttggccaccg cgtctacaag aactacgacc cccgcgccag gatcgtcaag gagcaggccg acaagatcct gtccaagctc ggcggcgatg actccctgct gggtatcgcg aaggagctcg aagaggcggc gttgaccgac gactacttca tcgagcgcaa gctctacccg aacgtcgact tctacaccgg cctgatctac cgtgccctcg gcttccccac ccggatgttc accgtgctgt ttgcgctggg ccggctgccg ggctggatcg cgcactggcg cgaaatgcac gacgagggcg acagcaagat cgggcgtccg cgccagatct acaccggtta caccgagcgc gactacgtcg cgatggacgg gaaacagcac gaacaagttc tgcagccaag cttctcgagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaa
Details for MymaA.00896.a.A1.PS00876
PURIFICATION DATe: 11/12/2010
CONCENTRATION: 13.99mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Low Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 2
VIAL VOLUME: 200µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 81
Protocol Notes
notes unavailable
Validated AA Sequence
MVSDTDDTAT LKYPGGEIDL QIAHATEGAD GIALGPLLSK TGHTTFDNGF VNTASCKSSI TYIDGDAGIL RYRGYPIDQL AEKSTFIEVC YLLIYGELPN TDQLAQFTGR IQRHTMLHED LKRFFDGFPR NAHPMPVLSS VVNALSAYYQ DALDPMDSEQ VELSTIRLLA KLPTIAAYAY KKSVGQPFLY PENSLTLVEN FLRMTFGFPA EPYHPDPEVV RALDMLFILH ADHEQNCSTS TVRLVGSSRA NLFTSISGGI NALWGPLHGG ANQAVLEMLE QIRDSGDDVS EFVRKVKNRE AGVKLMGFGH RVYKNYDPRA RIVKEQADKI LSKLGGDDSL LGIAKELEEA ALTDDYFIER KLYPNVDFYT
Validated NT Sequence
gncggtgtag aagtcgacgt tcgggtagag cttgcgctcg atgaagtagt cgtcggtcaa cgccgcctct tcgagctcct tcgcgatacc cagcagggag tcatcgccgc cgagcttgga caggatcttg tcggcctgct ccttgacgat cctggcgcgg gggtcgtagt tcttgtagac gcggtggcca aagcccatca gcttgacgcc ggcttcgcgg ttcttcacct tgcggacgaa ctcgctcacg tcgtcgccgc tgtcgcggat ctgctcgagc atctcgagca ccgcctggtt ggcaccgccg tgcagcgggc cccagagggc gttgatgccg cccgagatcg aggtgaacag gttggcccgg gaggaaccca ccagccgcac cgtggaggtc gagcagttct gctcgtggtc ggcgtgcagg atgaacagca tgtccagcgc ccgcaccacc tcagggtcgg ggtggtaggg ctcggcgggg aagccgaacg tcatccgcaa gaagttctcg accagcgtca gggagttctc cgggtagagg aacggctggc caaccgactt cttgtaggcg taggcggcga tggtcggcag cttggccagc aaccggatcg tcgagagctc aacctgctcg ctgtccatcg ggtccagcgc gtcctggtag tacgccgaga gcgcgttgac cacgctggac agcactggca tcgggtgggc gttgcgaggg aaaccgtcga agaaccgctt gaggtcctcg tgcagcatgg tgtggcgctg gatccggccg gtgaactggg cgagctggtc ggtgttgggc aactcgccgt agatcagcag gtagcagacc tcgatgaagg tcgacttctc cgccagctgg tcgatcgggt atccgcggta gcgcagaatg ccggcgtcac cgtcgatgta ggtgattgag cttttgcacg acgcggtatt gacgaagccg ttgtcgaacg tggtgtgtcc ggtcttggac agcagcgggc cgagcgcaat gccgtcagca ccctcggtgg catgggcgat ctgcaggtcg atctcgcccc ccgggtactt cagagttgcg gtgtcgtcgg tgtcggacac catcgaacca ggaccctggg tctgagcttc caggg
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVSDTDDTA TLKYPGGEID LQIAHATEGA DGIALGPLLS KTGHTTFDNG FVNTASCKSS ITYIDGDAGI LRYRGYPIDQ LAEKSTFIEV CYLLIYGELP NTDQLAQFTG RIQRHTMLHE DLKRFFDGFP RNAHPMPVLS SVVNALSAYY QDALDPMDSE QVELSTIRLL AKLPTIAAYA YKKSVGQPFL YPENSLTLVE NFLRMTFGFP AEPYHPDPEV VRALDMLFIL HADHEQNCST STVRLVGSSR ANLFTSISGG INALWGPLHG GANQAVLEML EQIRDSGDDV SEFVRKVKNR EAGVKLMGFG HRVYKNYDPR ARIVKEQADK ILSKLGGDDS LLGIAKELEE AALTDDYFIE RKLYPNVDFY TGLIYRALGF PTRMFTVLFA LGRLPGWIAH WREMHDEGDS KIGRPRQIYT GYTERDYVAM DG
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGTGTCCGAC ACCGACGACA CCGCAACTCT GAAGTACCCG GGGGGCGAGA TCGACCTGCA GATCGCCCAT GCCACCGAGG GTGCTGACGG CATTGCGCTC GGCCCGCTGC TGTCCAAGAC CGGACACACC ACGTTCGACA ACGGCTTCGT CAATACCGCG TCGTGCAAAA GCTCAATCAC CTACATCGAC GGTGACGCCG GCATTCTGCG CTACCGCGGA TACCCGATCG ACCAGCTGGC GGAGAAGTCG ACCTTCATCG AGGTCTGCTA CCTGCTGATC TACGGCGAGT TGCCCAACAC CGACCAGCTC GCCCAGTTCA CCGGCCGGAT CCAGCGCCAC ACCATGCTGC ACGAGGACCT CAAGCGGTTC TTCGACGGTT TCCCTCGCAA CGCCCACCCG ATGCCAGTGC TGTCCAGCGT GGTCAACGCG CTCTCGGCGT ACTACCAGGA CGCGCTGGAC CCGATGGACA GCGAGCAGGT TGAGCTCTCG ACGATCCGGT TGCTGGCCAA GCTGCCGACC ATCGCCGCCT ACGCCTACAA GAAGTCGGTT GGCCAGCCGT TCCTCTACCC GGAGAACTCC CTGACGCTGG TCGAGAACTT CTTGCGGATG ACGTTCGGCT TCCCCGCCGA GCCCTACCAC CCCGACCCTG AGGTGGTGCG GGCGCTGGAC ATGCTGTTCA TCCTGCACGC CGACCACGAG CAGAACTGCT CGACCTCCAC GGTGCGGCTG GTGGGTTCCT CCCGGGCCAA CCTGTTCACC TCGATCTCGG GCGGCATCAA CGCCCTCTGG GGCCCGCTGC ACGGCGGTGC CAACCAGGCG GTGCTCGAGA TGCTCGAGCA GATCCGCGAC AGCGGCGACG ACGTGAGCGA GTTCGTCCGC AAGGTGAAGA ACCGCGAAGC CGGCGTCAAG CTGATGGGCT TTGGCCACCG CGTCTACAAG AACTACGACC CCCGCGCCAG GATCGTCAAG GAGCAGGCCG ACAAGATCCT GTCCAAGCTC GGCGGCGATG ACTCCCTGCT GGGTATCGCG AAGGAGCTCG AAGAGGCGGC GTTGACCGAC GACTACTTCA TCGAGCGCAA GCTCTACCCG AACGTCGACT TCTACACCGG CCTGATCTAC CGTGCCCTCG GCTTCCCCAC CCGGATGTTC ACCGTGCTGT TTGCGCTGGG CCGGCTGCCG GGCTGGATCG CGCACTGGCG CGAAATGCAC GACGAGGGCG ACAGCAAGAT CGGGCGTCCG CGCCAGATCT ACACCGGTTA CACCGAGCGC GACTACGTCG CGATGGACGG GAAACAGCAC GAACAAGTTC TGCAGCCAAG CTTCTCGAGG ATCCGGCTGC TAACAAAGCC CGAAAGGAAG CTGAGTTGGC TGCTGCCACC GCTGAGCAAT AACTAGCATA ACCCCTTGGG GCCTCTAAAC GGGTCTTGAG GGGTTTTTTG CTGAAAGGAG GAACTATATC CGGATATCCA CAGGACGGGT GTGGTCGCCA TGATCGCGTA GTCGATAGTG GCTCCAAGTA GCGAAGCGAG CAGGACTGGG CGGCGGCCAA AGCGGTCGGA CAGTGCTCCG AGAACGGGTG CGCATAGAAA TTGCATCAAC GCATATAGCG CTAGCAGCAC GCCATAGTGA CTGGCGATGC TGTCGGAATG GACGATATCC CGCAAGAGGC CCGGCAGTAC CGGCATAACC AAGCCTATGC CTACAGCATC CAGGGTGACG GTGCCGAGGA TGACGATGAG CGCATTGTTA GATTTCATAC ACGGTGCCTG ACTGCGTTAG CAATTTAACT GTGATAAACT ACCGCATTAA AGCTTATCGA TGATAAGCTG TCAAACATGA GAA