MymaA.00829.a
Enoyl-CoA hydratase, EchA3
| CENTER ID: | MymaA.00829.a |
| ORGANISM: | Mycobacterium marinum ATCC BAA-535 / M |
| ASSOCIATED DISEASE: | Cutaneous ulcerations, deep abscesses |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | False |
| NIH RISK GROUP: | 2 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
IIIC |
Ordering Clones & Proteins
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| MymaA.00829.a.A1.GE30079 | Full length( MymaA.00829.a ) | 1 | 231 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| MymaA.00829.a.A1.PW29951 | Full length( MymaA.00829.a ) | 1 | 231 |
Structures
3R6H
DEPOSITED: 3/21/2011
DETERMINATION: XRay
CLONE: MymaA.00829.a.A1.GE30079
PROTEIN: MymaA.00829.a.A1.PW29951
Publications by SSGCID
Increasing the structural coverage of tuberculosis drug targets.
Abendroth J, Abramov A, Armour B, Barrett LK, Baugh L, Begley DW, Buchko GW, Choi R, Clifton MC, Dieterich SH, Dranow DM, Edwards TE, Fairman JW, Ferrell M, Fox D, Gardberg AS, Hewitt SN, Lorimer D, Lyons-Abbott S, Mundt E, Muruthi MM, Myers J, Myler PJ, Napuli AJ, Phan I, Sekar A, Serbzhinskiy D, Stacy R, Staker BL, Stewart LJ, Taylor BM, Thompkins K, Tran N, Van Voorhis WC, Zhang Y
Tuberculosis (Edinb) - 2014
volume 95, issue 2, pages 142-8
PMID: 25613812; PMCID: PMC4361283
PDB:
3GVC,
3GVG,
3GWC,
3H7F,
3H81,
3HE2,
3HWI,
3HWK,
3HZG,
3ICO,
3KHP,
3LLS,
3MOY,
3MPZ,
3MYB,
3NDN,
3NDO,
3NF4,
3NG3,
3NJD,
3NWO,
3O0M,
3O38,
3OC6,
3OC7,
3OEC,
3OI9,
3OKS,
3OL3,
3OL4,
3OME,
3P0T,
3P2Y,
3P4I,
3P4T,
3P5M,
3P85,
3PE8,
3PGX,
3PK0,
3PPI,
3PXX,
3PZY,
3Q1T,
3Q8N,
3QBP,
3QDF,
3QH4,
3QHA,
3QIV,
3QK8,
3QKA,
3QLJ,
3QMJ,
3QRE,
3QUA,
3QUV,
3QXI,
3QXZ,
3QYR,
3R0O,
3R1I,
3R1J,
3R20,
3R2N,
3R4T,
3R6H,
3R6O,
3R7K,
3R8C,
3R9P,
3R9Q,
3R9R,
3R9S,
3R9T,
3RD5,
3RD7,
3RD8,
3RFQ,
3RIH,
3RR2,
3RR6,
3RRP,
3RRV,
3RSI,
3RV2,
3S55,
3S82,
3SBX,
3SF6,
3SLL,
3SVK,
3SVT,
3SWO,
3SWT,
3SWX,
3SX2,
3T3W,
3T7C,
3TAV,
3TCR,
3TDE,
3TJR,
3TL3,
3TLF,
3TRR,
3TSC,
3TX2,
3TZQ,
3TZU,
3U0A,
3U0B,
3UCX,
3UVE,
4DI1,
4DIE,
4DQ8,
4DXL,
4ED4,
4EGE,
4EGF,
4EMD,
4EO9,
4EYE,
4F3W,
4F47,
4FFC,
4GK6,
4HDT,
4HR3,
4I1Y,
4IJN,
4IV6,
4IZ9,
4J5I,
4KAM,
4LGV,
4O2D,
5EJ2
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| OrthoMCL: | OG5_131454 |
| PATRIC ID: | fig|216594.6.peg.1026 |
| RefSeq: | YP_001849274.1 |
| UniProt: | B2HSG1 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSGPVTYTHD DAIGVIRMDD GKVNVLGPTM QQALNEAIDA ADRDNVGALV IAGNHRVFSG GFDLKVLTSG EAKPAIDMLR GGFELSYRLL SYPKPVVIAC TGHAIAMGAF LLCSGDHRVA AHAYNVQANE VAIGMTIPYA AMEVLKLRLT PSAYQQAAGL AKTFFGETAL AAGFIDEISL PEVVLSRAEE AAREFAGLNQ QAHNATKLRA RAEALKAIRA GIDGIEAEFG L
NT Sequence
ATGAGCGGGC CGGTCACCTA CACCCACGAC GACGCCATCG GCGTCATCCG AATGGATGAC GGCAAAGTCA ACGTGCTGGG CCCGACCATG CAGCAGGCAC TCAACGAAGC GATTGACGCC GCCGACCGCG ACAACGTCGG TGCGCTGGTG ATCGCGGGCA ACCATCGAGT GTTCAGCGGC GGCTTCGACC TCAAGGTGCT GACTTCGGGC GAGGCAAAGC CCGCGATCGA CATGCTCCGC GGCGGATTCG AGCTGTCCTA CCGGCTGTTG TCCTACCCCA AGCCGGTGGT GATCGCCTGC ACCGGCCACG CGATCGCGAT GGGCGCATTC CTGTTGTGCT CCGGCGACCA CCGGGTGGCC GCCCACGCCT ACAACGTCCA GGCCAACGAG GTCGCGATCG GTATGACGAT CCCCTACGCG GCGATGGAAG TGTTGAAGCT GCGGCTGACC CCGTCGGCAT ACCAGCAAGC CGCCGGACTA GCCAAGACCT TCTTCGGCGA AACCGCGCTG GCGGCCGGAT TCATCGATGA GATCTCGCTT CCCGAGGTGG TGCTCAGCCG CGCCGAAGAG GCCGCGCGCG AATTCGCCGG ACTCAATCAG CAGGCCCACA ACGCCACCAA GTTGCGGGCC CGCGCTGAGG CACTCAAGGC CATCCGCGCC GGCATCGACG GGATCGAAGC AGAGTTCGGA CTG
Details for MymaA.00829.a.A1.GE30079
| HARVESTED ON: | 8/10/2010 |
| SEQUENCED ON: | 8/16/2010 |
| EXPECTED MW: | 26kDa |
| OBSERVED MW: | 26kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Many (50-100) |
| TOTAL EXPRESSION LEVEL: | Low Expression |
| SOLUBLE EXPRESSION LEVEL | High Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass with incomplete coverage |
| PERCENT IDENTITY: | 99 |
| PERCENT COVERAGE: | 100 |
Validated AA Sequence
MAHHHHHHMG TXEXQTQGPG SMSGPVTYTH DDAIGVIRMD DGKVNVLGPT MQQALNEAID AADRDNVGAL VIAGNHRVFS GGFDLKVLTS GEAKPAIDML RGGFELSYRL LSYPKPVVIA CTGHAIAMGA FLLCSGDHRV AAHAYNVQAN EVAIGMTIPY AAMEVLKLRL TPSAYQQAAG LAKTFFGETA LAAGFIDEIS LPEVVLSRAE EAAREFAGLN QQAHNATKLR ARAEALKAIR AGIDGIEAEF G
Validated NT Sequence
tcnttcgngc tttgttagca gccggancct cgagaagctt ggctgcagaa cttgttcgtg ctgtttatta tccgaactct gcttcgatcc cgtcgatgcc ggcgcggatg gccttgagtg cctcagcgcg ggcccgcaac ttggtggcgt tgtgggcctg ctgattgagt ccggcgaatt cgcgcgcggc ctcttcggcg cggctgagca ccacctcggg aagcgagatc tcatcgatga atccggccgc cagcgcggtt tcgccgaaga aggtcttggc tagtccggcg gcttgctggt atgccgacgg ggtcagccgc agcttcaaca cttccatcgc cgcgtagggg atcgtcatac cgatcgcgac ctcgttggcc tggacgttgt aggcgtgggc ggccacccgg tggtcgccgg agcacaacag gaatgcgccc atcgcgatcg cgtggccggt gcaggcgatc accaccggct tggggtagga caacagccgg taggacagct cgaatccgcc gcggagcatg tcgatcgcgg gctttgcctc gcccgaagtc agcaccttga ggtcgaagcc gccgctgaac actcgatggt tgcccgcgat caccagcgca ccgacgttgt cgcggtcggc ggcgtcaatc gcttcgttga gtgcctgctg catggtcggg cccagcacgt tgactttgcc gtcatccatt cggatgacgc cgatggcgtc gtcgtgggtg taggtgaccg gcccgctcat cgaaccagga ccctgggtct gancttccan ggtacccata tgatggtgat ggtgatgagc catggtatat ctccttctta aagttaaaca aaattatttc tagagggaaa ccgttgtggt nnnnnnnnnn nnnnnnnnna nnggggnnnn nntnnntngn ttnnnnnggg nnnnnntnng nncnnnnnng nnnnnnnnnn gncnnnnnnn ncnnnnnnnn nnnnnnngnn nnngnnnncn nnggnngnnt nannangnnn nnaca
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMSGPVTYTH DDAIGVIRMD DGKVNVLGPT MQQALNEAID AADRDNVGAL VIAGNHRVFS GGFDLKVLTS GEAKPAIDML RGGFELSYRL LSYPKPVVIA CTGHAIAMGA FLLCSGDHRV AAHAYNVQAN EVAIGMTIPY AAMEVLKLRL TPSAYQQAAG LAKTFFGETA LAAGFIDEIS LPEVVLSRAE EAAREFAGLN QQAHNATKLR ARAEALKAIR AGIDGIEAEF G
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gagcgggccg gtcacctaca cccacgacga cgccatcggc gtcatccgaa tggatgacgg caaagtcaac gtgctgggcc cgaccatgca gcaggcactc aacgaagcga ttgacgccgc cgaccgcgac aacgtcggtg cgctggtgat cgcgggcaac catcgagtgt tcagcggcgg cttcgacctc aaggtgctga cttcgggcga ggcaaagccc gcgatcgaca tgctccgcgg cggattcgag ctgtcctacc ggctgttgtc ctaccccaag ccggtggtga tcgcctgcac cggccacgcg atcgcgatgg gcgcattcct gttgtgctcc ggcgaccacc gggtggccgc ccacgcctac aacgtccagg ccaacgaggt cgcgatcggt atgacgatcc cctacgcggc gatggaagtg ttgaagctgc ggctgacccc gtcggcatac cagcaagccg ccggactagc caagaccttc ttcggcgaaa ccgcgctggc ggccggattc atcgatgaga tctcgcttcc cgaggtggtg ctcagccgcg ccgaagaggc cgcgcgcgaa ttcgccggac tcaatcagca ggcccacaac gccaccaagt tgcgggcccg cgctgaggca ctcaaggcca tccgcgccgg catcgacggg atcgaagcag agttcggaaa acagcacgaa caagttctgc agccaagctt ctcgaggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa
Details for MymaA.00829.a.A1.PW29951
| PURIFICATION DATe: | 10/25/2010 |
| CONCENTRATION: | 20.47mg/ml |
| OBSERVED MW: | data unavailable |
| EXPRESSION LEVEL: | Moderate Expression |
| PROTEIN PURIFICATION BUFFER: | 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 18 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | 99 |
| PERCENT COVERAGE: | 100 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TXEXQTQGPG SMSGPVTYTH DDAIGVIRMD DGKVNVLGPT MQQALNEAID AADRDNVGAL VIAGNHRVFS GGFDLKVLTS GEAKPAIDML RGGFELSYRL LSYPKPVVIA CTGHAIAMGA FLLCSGDHRV AAHAYNVQAN EVAIGMTIPY AAMEVLKLRL TPSAYQQAAG LAKTFFGETA LAAGFIDEIS LPEVVLSRAE EAAREFAGLN QQAHNATKLR ARAEALKAIR AGIDGIEAEF G
Validated NT Sequence
tcnttcgngc tttgttagca gccggancct cgagaagctt ggctgcagaa cttgttcgtg ctgtttatta tccgaactct gcttcgatcc cgtcgatgcc ggcgcggatg gccttgagtg cctcagcgcg ggcccgcaac ttggtggcgt tgtgggcctg ctgattgagt ccggcgaatt cgcgcgcggc ctcttcggcg cggctgagca ccacctcggg aagcgagatc tcatcgatga atccggccgc cagcgcggtt tcgccgaaga aggtcttggc tagtccggcg gcttgctggt atgccgacgg ggtcagccgc agcttcaaca cttccatcgc cgcgtagggg atcgtcatac cgatcgcgac ctcgttggcc tggacgttgt aggcgtgggc ggccacccgg tggtcgccgg agcacaacag gaatgcgccc atcgcgatcg cgtggccggt gcaggcgatc accaccggct tggggtagga caacagccgg taggacagct cgaatccgcc gcggagcatg tcgatcgcgg gctttgcctc gcccgaagtc agcaccttga ggtcgaagcc gccgctgaac actcgatggt tgcccgcgat caccagcgca ccgacgttgt cgcggtcggc ggcgtcaatc gcttcgttga gtgcctgctg catggtcggg cccagcacgt tgactttgcc gtcatccatt cggatgacgc cgatggcgtc gtcgtgggtg taggtgaccg gcccgctcat cgaaccagga ccctgggtct gancttccan ggtacccata tgatggtgat ggtgatgagc catggtatat ctccttctta aagttaaaca aaattatttc tagagggaaa ccgttgtggt nnnnnnnnnn nnnnnnnnna nnggggnnnn nntnnntngn ttnnnnnggg nnnnnntnng nncnnnnnng nnnnnnnnnn gncnnnnnnn ncnnnnnnnn nnnnnnngnn nnngnnnncn nnggnngnnt nannangnnn nnaca
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMSGPVTYTH DDAIGVIRMD DGKVNVLGPT MQQALNEAID AADRDNVGAL VIAGNHRVFS GGFDLKVLTS GEAKPAIDML RGGFELSYRL LSYPKPVVIA CTGHAIAMGA FLLCSGDHRV AAHAYNVQAN EVAIGMTIPY AAMEVLKLRL TPSAYQQAAG LAKTFFGETA LAAGFIDEIS LPEVVLSRAE EAAREFAGLN QQAHNATKLR ARAEALKAIR AGIDGIEAEF G
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GAGCGGGCCG GTCACCTACA CCCACGACGA CGCCATCGGC GTCATCCGAA TGGATGACGG CAAAGTCAAC GTGCTGGGCC CGACCATGCA GCAGGCACTC AACGAAGCGA TTGACGCCGC CGACCGCGAC AACGTCGGTG CGCTGGTGAT CGCGGGCAAC CATCGAGTGT TCAGCGGCGG CTTCGACCTC AAGGTGCTGA CTTCGGGCGA GGCAAAGCCC GCGATCGACA TGCTCCGCGG CGGATTCGAG CTGTCCTACC GGCTGTTGTC CTACCCCAAG CCGGTGGTGA TCGCCTGCAC CGGCCACGCG ATCGCGATGG GCGCATTCCT GTTGTGCTCC GGCGACCACC GGGTGGCCGC CCACGCCTAC AACGTCCAGG CCAACGAGGT CGCGATCGGT ATGACGATCC CCTACGCGGC GATGGAAGTG TTGAAGCTGC GGCTGACCCC GTCGGCATAC CAGCAAGCCG CCGGACTAGC CAAGACCTTC TTCGGCGAAA CCGCGCTGGC GGCCGGATTC ATCGATGAGA TCTCGCTTCC CGAGGTGGTG CTCAGCCGCG CCGAAGAGGC CGCGCGCGAA TTCGCCGGAC TCAATCAGCA GGCCCACAAC GCCACCAAGT TGCGGGCCCG CGCTGAGGCA CTCAAGGCCA TCCGCGCCGG CATCGACGGG ATCGAAGCAG AGTTCGGAAA ACAGCACGAA CAAGTTCTGC AGCCAAGCTT CTCGAGGATC CGGCTGCTAA CAAAGCCCGA AAGGAAGCTG AGTTGGCTGC TGCCACCGCT GAGCAATAAC TAGCATAACC CCTTGGGGCC TCTAAACGGG TCTTGAGGGG TTTTTTGCTG AAAGGAGGAA CTATATCCGG ATATCCACAG GACGGGTGTG GTCGCCATGA TCGCGTAGTC GATAGTGGCT CCAAGTAGCG AAGCGAGCAG GACTGGGCGG CGGCCAAAGC GGTCGGACAG TGCTCCGAGA ACGGGTGCGC ATAGAAATTG CATCAACGCA TATAGCGCTA GCAGCACGCC ATAGTGACTG GCGATGCTGT CGGAATGGAC GATATCCCGC AAGAGGCCCG GCAGTACCGG CATAACCAAG CCTATGCCTA CAGCATCCAG GGTGACGGTG CCGAGGATGA CGATGAGCGC ATTGTTAGAT TTCATACACG GTGCCTGACT GCGTTAGCAA TTTAACTGTG ATAAACTACC GCATTAAAGC TTATCGATGA TAAGCTGTCA AACATGAGAA