MymaA.00814.a

O-methyltransferase

CENTER ID: MymaA.00814.a
ORGANISM: Mycobacterium marinum ATCC BAA-535 / M
ASSOCIATED DISEASE: Cutaneous ulcerations, deep abscesses
CURRENT STATUS: crystallized
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MymaA.00814.a.A2.GE30078 Mature protein( MymaA.00814.a ) 20 250
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MymaA.00814.a.A2.PS00976 Mature protein( MymaA.00814.a ) 20 250
MymaA.00814.a.A2.PS00977 Mature protein( MymaA.00814.a ) 20 250

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|216594.6.peg.2670
RefSeq: YP_001850813.1
UniProt: B2HR72

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MRLNARFAPL RWSVLRLALA FRTLLRTGQV GDGREAAAVD YVLEHARTGD IDDVLATIDK FAYEKSILVN VGDEKGLLLD AAVRRADPAL ALELGTYCGY GALRIARAAP QAKVYSVELA EANAANARQI WAHAGVADRV TCVVGTIGDG GRTLDALATE HGFAPGALDF VFVDHDKKAY LDDLQSILDR EWLHRGSIVV ADNVKVPGAP EYHEYMRHHQ GTLWDTTEHK THLEYQTLVS DLVLESDYLG
NT Sequence
ATGAGGCTCA ACGCACGCTT CGCTCCGTTG CGGTGGTCGG TATTGCGCCT GGCACTGGCA TTTCGCACCC TGCTCAGAAC GGGCCAGGTC GGCGACGGCC GCGAGGCCGC GGCCGTCGAC TATGTGCTCG AGCACGCCCG CACCGGCGAT ATCGACGACG TGCTGGCCAC CATCGACAAG TTCGCCTACG AAAAGTCCAT CCTGGTCAAC GTCGGCGACG AGAAGGGGCT GCTACTCGAT GCCGCGGTGC GACGCGCCGA TCCGGCACTG GCGCTGGAAC TGGGCACCTA CTGCGGGTAT GGCGCGCTGC GGATCGCCCG GGCCGCACCG CAGGCCAAGG TGTACTCCGT CGAACTTGCC GAAGCCAACG CCGCCAACGC CCGACAGATC TGGGCTCATG CCGGCGTTGC CGATCGCGTG ACATGTGTCG TGGGCACCAT TGGCGACGGC GGACGCACCC TGGACGCACT CGCGACCGAG CACGGATTCG CCCCCGGTGC GCTCGATTTC GTCTTTGTCG ATCACGACAA GAAGGCCTAC CTCGACGACC TGCAAAGCAT CCTGGACCGG GAGTGGCTGC ATCGGGGCTC GATCGTGGTG GCCGACAACG TCAAGGTGCC CGGCGCTCCC GAGTACCACG AATACATGCG GCACCATCAG GGCACGCTGT GGGACACCAC CGAGCACAAG ACCCACCTCG AGTACCAGAC CCTGGTATCG GATCTGGTGC TCGAATCCGA CTACTTGGGG
Details for MymaA.00814.a.A2.GE30078
HARVESTED ON: 8/10/2010
SEQUENCED ON: 8/16/2010
EXPECTED MW: 27kDa
OBSERVED MW: 28kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 99
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMAFRTLLRT GQVGDGREAA AVDYVLEHAR TGDIDDVLAT IDKFAYEKSI LVNVGDEKGL LLDAAVRRAD PALALELGTY CGYGALRIAR AAPQAKVYSV ELAEANAANA RQIWAHAGVA DRVTCVVGTI GDGGRTLDAL ATEHGFAPGA LDFVFVDHDK KAYLDDLQSI LDREWLHRGS IVVADNVKVP GAPEYHEYMR HHQGTLWDTT EHKTHLEYQT LVSDLVLESD
Validated NT Sequence
agtcggattc gagcaccaga tccgatacca gggtctggta ctcgaggtgg gtcttgtgct cggtggtgtc ccacagcgtg ccctgatggt gccgcatgta ttcgtggtac tcgggagcgc cgggcacctt gacgttgtcg gccaccacga tcgagccccg atgcagccac tcccggtcca ggatgctttg caggtcgtcg aggtaggcct tcttgtcgtg atcgacaaag acgaaatcga gcgcaccggg ggcgaatccg tgctcggtcg cgagtgcgtc cagggtgcgt ccgccgtcgc caatggtgcc cacgacacat gtcacgcgat cggcaacgcc ggcatgagcc cagatctgtc gggcgttggc ggcgttggct tcggcaagtt cgacggagta caccttggcc tgcggtgcgg cccgggcgat ccgcagcgcg ccatacccgc agtaggtgcc cagttccagc gccagtgccg gatcggcgcg tcgcaccgcg gcatcgagta gcagcccctt ctcgtcgccg acgttgacca ggatggactt ttcgtaggcg aacttgtcga tggtggccag cacgtcgtcg atatcgccgg tgcgggcgtg ctcgagcaca tagtcgacgg ccgcggcctc gcggccgtcg ccgacctggc ccgttctgag cagggtgcga aatgccatcg aaccaggacc ctgggtctga gcttccaggg tacccatatg atggtgatgg tgatgagcca t
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMAFRTLLRT GQVGDGREAA AVDYVLEHAR TGDIDDVLAT IDKFAYEKSI LVNVGDEKGL LLDAAVRRAD PALALELGTY CGYGALRIAR AAPQAKVYSV ELAEANAANA RQIWAHAGVA DRVTCVVGTI GDGGRTLDAL ATEHGFAPGA LDFVFVDHDK KAYLDDLQSI LDREWLHRGS IVVADNVKVP GAPEYHEYMR HHQGTLWDTT EHKTHLEYQT LVSDLVLESD YL
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggcatttcgc accctgctca gaacgggcca ggtcggcgac ggccgcgagg ccgcggccgt cgactatgtg ctcgagcacg cccgcaccgg cgatatcgac gacgtgctgg ccaccatcga caagttcgcc tacgaaaagt ccatcctggt caacgtcggc gacgagaagg ggctgctact cgatgccgcg gtgcgacgcg ccgatccggc actggcgctg gaactgggca cctactgcgg gtatggcgcg ctgcggatcg cccgggccgc accgcaggcc aaggtgtact ccgtcgaact tgccgaagcc aacgccgcca acgcccgaca gatctgggct catgccggcg ttgccgatcg cgtgacatgt gtcgtgggca ccattggcga cggcggacgc accctggacg cactcgcgac cgagcacgga ttcgcccccg gtgcgctcga tttcgtcttt gtcgatcacg acaagaaggc ctacctcgac gacctgcaaa gcatcctgga ccgggagtgg ctgcatcggg gctcgatcgt ggtggccgac aacgtcaagg tgcccggcgc tcccgagtac cacgaataca tgcggcacca tcagggcacg ctgtgggaca ccaccgagca caagacccac ctcgagtacc agaccctggt atcggatctg gtgctcgaat ccgactactt gaaacagcac gaacaagttc tgcagccaag cttctcgagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaa
Details for MymaA.00814.a.A2.PS00976
PURIFICATION DATe: 2/10/2011
CONCENTRATION: 11.13mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 1
VIAL VOLUME: 200µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 99
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMAFRTLLRT GQVGDGREAA AVDYVLEHAR TGDIDDVLAT IDKFAYEKSI LVNVGDEKGL LLDAAVRRAD PALALELGTY CGYGALRIAR AAPQAKVYSV ELAEANAANA RQIWAHAGVA DRVTCVVGTI GDGGRTLDAL ATEHGFAPGA LDFVFVDHDK KAYLDDLQSI LDREWLHRGS IVVADNVKVP GAPEYHEYMR HHQGTLWDTT EHKTHLEYQT LVSDLVLESD
Validated NT Sequence
agtcggattc gagcaccaga tccgatacca gggtctggta ctcgaggtgg gtcttgtgct cggtggtgtc ccacagcgtg ccctgatggt gccgcatgta ttcgtggtac tcgggagcgc cgggcacctt gacgttgtcg gccaccacga tcgagccccg atgcagccac tcccggtcca ggatgctttg caggtcgtcg aggtaggcct tcttgtcgtg atcgacaaag acgaaatcga gcgcaccggg ggcgaatccg tgctcggtcg cgagtgcgtc cagggtgcgt ccgccgtcgc caatggtgcc cacgacacat gtcacgcgat cggcaacgcc ggcatgagcc cagatctgtc gggcgttggc ggcgttggct tcggcaagtt cgacggagta caccttggcc tgcggtgcgg cccgggcgat ccgcagcgcg ccatacccgc agtaggtgcc cagttccagc gccagtgccg gatcggcgcg tcgcaccgcg gcatcgagta gcagcccctt ctcgtcgccg acgttgacca ggatggactt ttcgtaggcg aacttgtcga tggtggccag cacgtcgtcg atatcgccgg tgcgggcgtg ctcgagcaca tagtcgacgg ccgcggcctc gcggccgtcg ccgacctggc ccgttctgag cagggtgcga aatgccatcg aaccaggacc ctgggtctga gcttccaggg tacccatatg atggtgatgg tgatgagcca t
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMAFRTLLRT GQVGDGREAA AVDYVLEHAR TGDIDDVLAT IDKFAYEKSI LVNVGDEKGL LLDAAVRRAD PALALELGTY CGYGALRIAR AAPQAKVYSV ELAEANAANA RQIWAHAGVA DRVTCVVGTI GDGGRTLDAL ATEHGFAPGA LDFVFVDHDK KAYLDDLQSI LDREWLHRGS IVVADNVKVP GAPEYHEYMR HHQGTLWDTT EHKTHLEYQT LVSDLVLESD YL
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGCATTTCGC ACCCTGCTCA GAACGGGCCA GGTCGGCGAC GGCCGCGAGG CCGCGGCCGT CGACTATGTG CTCGAGCACG CCCGCACCGG CGATATCGAC GACGTGCTGG CCACCATCGA CAAGTTCGCC TACGAAAAGT CCATCCTGGT CAACGTCGGC GACGAGAAGG GGCTGCTACT CGATGCCGCG GTGCGACGCG CCGATCCGGC ACTGGCGCTG GAACTGGGCA CCTACTGCGG GTATGGCGCG CTGCGGATCG CCCGGGCCGC ACCGCAGGCC AAGGTGTACT CCGTCGAACT TGCCGAAGCC AACGCCGCCA ACGCCCGACA GATCTGGGCT CATGCCGGCG TTGCCGATCG CGTGACATGT GTCGTGGGCA CCATTGGCGA CGGCGGACGC ACCCTGGACG CACTCGCGAC CGAGCACGGA TTCGCCCCCG GTGCGCTCGA TTTCGTCTTT GTCGATCACG ACAAGAAGGC CTACCTCGAC GACCTGCAAA GCATCCTGGA CCGGGAGTGG CTGCATCGGG GCTCGATCGT GGTGGCCGAC AACGTCAAGG TGCCCGGCGC TCCCGAGTAC CACGAATACA TGCGGCACCA TCAGGGCACG CTGTGGGACA CCACCGAGCA CAAGACCCAC CTCGAGTACC AGACCCTGGT ATCGGATCTG GTGCTCGAAT CCGACTACTT GAAACAGCAC GAACAAGTTC TGCAGCCAAG CTTCTCGAGG ATCCGGCTGC TAACAAAGCC CGAAAGGAAG CTGAGTTGGC TGCTGCCACC GCTGAGCAAT AACTAGCATA ACCCCTTGGG GCCTCTAAAC GGGTCTTGAG GGGTTTTTTG CTGAAAGGAG GAACTATATC CGGATATCCA CAGGACGGGT GTGGTCGCCA TGATCGCGTA GTCGATAGTG GCTCCAAGTA GCGAAGCGAG CAGGACTGGG CGGCGGCCAA AGCGGTCGGA CAGTGCTCCG AGAACGGGTG CGCATAGAAA TTGCATCAAC GCATATAGCG CTAGCAGCAC GCCATAGTGA CTGGCGATGC TGTCGGAATG GACGATATCC CGCAAGAGGC CCGGCAGTAC CGGCATAACC AAGCCTATGC CTACAGCATC CAGGGTGACG GTGCCGAGGA TGACGATGAG CGCATTGTTA GATTTCATAC ACGGTGCCTG ACTGCGTTAG CAATTTAACT GTGATAAACT ACCGCATTAA AGCTTATCGA TGATAAGCTG TCAAACATGA GAA
Details for MymaA.00814.a.A2.PS00977
PURIFICATION DATe: 2/10/2011
CONCENTRATION: 8.49mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 2
VIAL VOLUME: 200µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 99
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMAFRTLLRT GQVGDGREAA AVDYVLEHAR TGDIDDVLAT IDKFAYEKSI LVNVGDEKGL LLDAAVRRAD PALALELGTY CGYGALRIAR AAPQAKVYSV ELAEANAANA RQIWAHAGVA DRVTCVVGTI GDGGRTLDAL ATEHGFAPGA LDFVFVDHDK KAYLDDLQSI LDREWLHRGS IVVADNVKVP GAPEYHEYMR HHQGTLWDTT EHKTHLEYQT LVSDLVLESD
Validated NT Sequence
agtcggattc gagcaccaga tccgatacca gggtctggta ctcgaggtgg gtcttgtgct cggtggtgtc ccacagcgtg ccctgatggt gccgcatgta ttcgtggtac tcgggagcgc cgggcacctt gacgttgtcg gccaccacga tcgagccccg atgcagccac tcccggtcca ggatgctttg caggtcgtcg aggtaggcct tcttgtcgtg atcgacaaag acgaaatcga gcgcaccggg ggcgaatccg tgctcggtcg cgagtgcgtc cagggtgcgt ccgccgtcgc caatggtgcc cacgacacat gtcacgcgat cggcaacgcc ggcatgagcc cagatctgtc gggcgttggc ggcgttggct tcggcaagtt cgacggagta caccttggcc tgcggtgcgg cccgggcgat ccgcagcgcg ccatacccgc agtaggtgcc cagttccagc gccagtgccg gatcggcgcg tcgcaccgcg gcatcgagta gcagcccctt ctcgtcgccg acgttgacca ggatggactt ttcgtaggcg aacttgtcga tggtggccag cacgtcgtcg atatcgccgg tgcgggcgtg ctcgagcaca tagtcgacgg ccgcggcctc gcggccgtcg ccgacctggc ccgttctgag cagggtgcga aatgccatcg aaccaggacc ctgggtctga gcttccaggg tacccatatg atggtgatgg tgatgagcca t
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMAFRTLLRT GQVGDGREAA AVDYVLEHAR TGDIDDVLAT IDKFAYEKSI LVNVGDEKGL LLDAAVRRAD PALALELGTY CGYGALRIAR AAPQAKVYSV ELAEANAANA RQIWAHAGVA DRVTCVVGTI GDGGRTLDAL ATEHGFAPGA LDFVFVDHDK KAYLDDLQSI LDREWLHRGS IVVADNVKVP GAPEYHEYMR HHQGTLWDTT EHKTHLEYQT LVSDLVLESD YL
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGCATTTCGC ACCCTGCTCA GAACGGGCCA GGTCGGCGAC GGCCGCGAGG CCGCGGCCGT CGACTATGTG CTCGAGCACG CCCGCACCGG CGATATCGAC GACGTGCTGG CCACCATCGA CAAGTTCGCC TACGAAAAGT CCATCCTGGT CAACGTCGGC GACGAGAAGG GGCTGCTACT CGATGCCGCG GTGCGACGCG CCGATCCGGC ACTGGCGCTG GAACTGGGCA CCTACTGCGG GTATGGCGCG CTGCGGATCG CCCGGGCCGC ACCGCAGGCC AAGGTGTACT CCGTCGAACT TGCCGAAGCC AACGCCGCCA ACGCCCGACA GATCTGGGCT CATGCCGGCG TTGCCGATCG CGTGACATGT GTCGTGGGCA CCATTGGCGA CGGCGGACGC ACCCTGGACG CACTCGCGAC CGAGCACGGA TTCGCCCCCG GTGCGCTCGA TTTCGTCTTT GTCGATCACG ACAAGAAGGC CTACCTCGAC GACCTGCAAA GCATCCTGGA CCGGGAGTGG CTGCATCGGG GCTCGATCGT GGTGGCCGAC AACGTCAAGG TGCCCGGCGC TCCCGAGTAC CACGAATACA TGCGGCACCA TCAGGGCACG CTGTGGGACA CCACCGAGCA CAAGACCCAC CTCGAGTACC AGACCCTGGT ATCGGATCTG GTGCTCGAAT CCGACTACTT GAAACAGCAC GAACAAGTTC TGCAGCCAAG CTTCTCGAGG ATCCGGCTGC TAACAAAGCC CGAAAGGAAG CTGAGTTGGC TGCTGCCACC GCTGAGCAAT AACTAGCATA ACCCCTTGGG GCCTCTAAAC GGGTCTTGAG GGGTTTTTTG CTGAAAGGAG GAACTATATC CGGATATCCA CAGGACGGGT GTGGTCGCCA TGATCGCGTA GTCGATAGTG GCTCCAAGTA GCGAAGCGAG CAGGACTGGG CGGCGGCCAA AGCGGTCGGA CAGTGCTCCG AGAACGGGTG CGCATAGAAA TTGCATCAAC GCATATAGCG CTAGCAGCAC GCCATAGTGA CTGGCGATGC TGTCGGAATG GACGATATCC CGCAAGAGGC CCGGCAGTAC CGGCATAACC AAGCCTATGC CTACAGCATC CAGGGTGACG GTGCCGAGGA TGACGATGAG CGCATTGTTA GATTTCATAC ACGGTGCCTG ACTGCGTTAG CAATTTAACT GTGATAAACT ACCGCATTAA AGCTTATCGA TGATAAGCTG TCAAACATGA GAA