MymaA.00797.a

Antibiotic-transport ATP-binding protein ABC transporter

CENTER ID: MymaA.00797.a
ORGANISM: Mycobacterium marinum ATCC BAA-535 / M
ASSOCIATED DISEASE: Cutaneous ulcerations, deep abscesses
CURRENT STATUS: expressed
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MymaA.00797.a.A1.GE34943 Full length( MymaA.00797.a ) 1 299
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|216594.6.peg.2163
RefSeq: YP_001850331.1
UniProt: B2HM12

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MRDPEPAVSG AVGEVIRVRG LTFAYPKAST PAVRGMDFSV GRGEIFGFLG PSGAGKSTTQ RILIGLLRGH GGEVAVWGKD PASWGSDYYE RIGVSFELPN HYQKLTGLEN LQFFASLYSA PTLDPMQLLE AVGLADHADT RAGKYSKGMQ MRLTFARALL NDPELLFLDE PTSGMDPVNA RSVKNMIMDL KARGRTVFLT THDMATADEL CDRVAFVVDG SIVVSDRPAE LKIARSQRLV TVEYRGNGGG LSAAEFSLDT LADDPAFHAV LREHQVETIH SREASLEDVF VEVTGRRLS
NT Sequence
ATGCGCGACC CCGAGCCGGC CGTGTCCGGC GCCGTGGGCG AGGTGATCCG GGTCCGTGGA CTCACCTTCG CGTATCCGAA AGCGTCGACA CCCGCGGTGC GCGGCATGGA TTTCAGTGTC GGCCGAGGTG AAATCTTCGG CTTTCTCGGT CCCAGCGGTG CGGGCAAGTC GACGACGCAA CGAATTCTGA TCGGGCTACT GCGTGGCCAC GGCGGTGAAG TGGCGGTGTG GGGCAAAGAC CCGGCGAGCT GGGGCTCCGA CTACTACGAG CGCATTGGCG TGTCGTTCGA GTTGCCCAAT CACTATCAGA AACTCACCGG GCTGGAGAAC CTGCAGTTCT TCGCCTCGCT CTACAGCGCG CCGACGCTGG ATCCGATGCA GCTACTCGAG GCGGTCGGAT TGGCCGACCA CGCAGATACC CGGGCGGGCA AGTACTCCAA AGGCATGCAG ATGCGCCTGA CGTTCGCCAG GGCGCTACTC AACGACCCCG AGTTGCTCTT CCTGGACGAA CCGACTTCGG GGATGGATCC GGTGAACGCC CGCAGCGTGA AGAACATGAT CATGGATCTG AAGGCACGCG GGCGCACGGT GTTTCTCACC ACACACGACA TGGCGACGGC GGACGAGCTG TGTGACCGGG TGGCCTTTGT CGTCGACGGG AGCATCGTCG TGTCTGACCG GCCCGCCGAG CTGAAGATCG CCCGCAGCCA GCGCCTGGTC ACGGTCGAAT ATCGCGGCAA CGGCGGTGGT TTGAGCGCCG CGGAGTTTTC GCTGGACACG CTGGCCGACG ACCCGGCATT TCACGCGGTG CTGCGCGAAC ATCAGGTCGA GACGATCCAC AGCAGGGAAG CCAGCCTCGA GGACGTATTC GTCGAAGTCA CCGGCCGGCG GTTGTCG
Details for MymaA.00797.a.A1.GE34943
HARVESTED ON: 4/17/2012
SEQUENCED ON: 4/17/2012
EXPECTED MW: 34kDa
OBSERVED MW: 34kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 97
PERCENT COVERAGE: 92
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMRDPEPAVS GAVGEVIRVR GLTFAYPKAS TPAVRGMDFS VGRGEIFGFL GPSGAGKSTT QRILIGLLRG HGGEVAVWGK DPASWGSDYY ERIGVSFELP NHYQKLTGLE NLQFFASLYS APTLDPMQLL EAVGLADHAD TRAGKYSKGM QMRLTFARAL LNDPELLFLD EPTSGMDPVN ARSVKNMIMD LKARGRTVFL TTHDMATAXE LCDRVAFVVD GSIVVSXRPA ELXXARSQRL VTVEYRGNGG GLSAAXFSLD TLADDPAFXA VLREX
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgcgcg accccgagcc ggccgtgtcc ggcgccgtgg gcgaggtgat ccgggtccgt ggactcacct tcgcgtatcc gaaagcgtcg acacccgcgg tgcgcggcat ggatttcagt gtcggccgag gtgaaatctt cggctttctc ggtcccagcg gtgcgggcaa gtcgacgacg caacgaattc tgatcgggct actgcgtggc cacggcggtg aagtggcggt gtggggcaaa gacccggcga gctggggctc cgactactac gagcgcattg gcgtgtcgtt cgagttgccc aatcactatc agaaactcac cgggctggag aacctgcagt tcttcgcctc gctctacagc gcgccgacgc tggatccgat gcagctactc gaggcggtcg gattggccga ccacgcagat acccgggcgg gcaagtactc caaaggcatg cagatgcgcc tgacgttcgc cagggcgcta ctcaacgacc ccgagttgct cttcctggac gaaccgactt cggggatgga tccggtgaac gcccgcagcg tgaagaacat gatcatggat ctgaaggcac gcgggcgcac ggtgtttctc accacacacg acatggcgac ggcggangag ctgtgtgacc gggtggcctt tgtcgtcgac gggagcatcg tcgtgtctga ncggcccgcc gagctgaana ncgcccgcag ccagcgcctg gtcacggtcg aatatcgcgg caacggcggt ggtttgagcg ccgcggantt ttcgctggac acnctggccg acgacccggc ntttcncgcg gtgctgcgcg aacntc
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMRDPEPAVS GAVGEVIRVR GLTFAYPKAS TPAVRGMDFS VGRGEIFGFL GPSGAGKSTT QRILIGLLRG HGGEVAVWGK DPASWGSDYY ERIGVSFELP NHYQKLTGLE NLQFFASLYS APTLDPMQLL EAVGLADHAD TRAGKYSKGM QMRLTFARAL LNDPELLFLD EPTSGMDPVN ARSVKNMIMD LKARGRTVFL TTHDMATADE LCDRVAFVVD GSIVVSDRPA ELKIARSQRL VTVEYRGNGG GLSAAEFSLD TLADDPAFHA VLREHQVETI HSREASLEDV FVEVTGRRL
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gcgcgacccc gagccggccg tgtccggcgc cgtgggcgag gtgatccggg tccgtggact caccttcgcg tatccgaaag cgtcgacacc cgcggtgcgc ggcatggatt tcagtgtcgg ccgaggtgaa atcttcggct ttctcggtcc cagcggtgcg ggcaagtcga cgacgcaacg aattctgatc gggctactgc gtggccacgg cggtgaagtg gcggtgtggg gcaaagaccc ggcgagctgg ggctccgact actacgagcg cattggcgtg tcgttcgagt tgcccaatca ctatcagaaa ctcaccgggc tggagaacct gcagttcttc gcctcgctct acagcgcgcc gacgctggat ccgatgcagc tactcgaggc ggtcggattg gccgaccacg cagatacccg ggcgggcaag tactccaaag gcatgcagat gcgcctgacg ttcgccaggg cgctactcaa cgaccccgag ttgctcttcc tggacgaacc gacttcgggg atggatccgg tgaacgcccg cagcgtgaag aacatgatca tggatctgaa ggcacgcggg cgcacggtgt ttctcaccac acacgacatg gcgacggcgg acgagctgtg tgaccgggtg gcctttgtcg tcgacgggag catcgtcgtg tctgaccggc ccgccgagct gaagatcgcc cgcagccagc gcctggtcac ggtcgaatat cgcggcaacg gcggtggttt gagcgccgcg gagttttcgc tggacacgct ggccgacgac ccggcatttc acgcggtgct gcgcgaacat caggtcgaga cgatccacag cagggaagcc agcctcgagg acgtattcgt cgaagtcacc ggccggcggt tgaaacagca cgaacaagtt ctgcagccaa gcttctcgag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaa