MymaA.00629.f

Bifunctional enzyme MbtA: salicyl-AMP ligase (SAL-AMP ligase) + salicyl-S-ACP synthetase

CENTER ID: MymaA.00629.f
ORGANISM: Mycobacterium marinum ATCC BAA-535 / M
ASSOCIATED DISEASE: Cutaneous ulcerations, deep abscesses
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MymaA.00629.f.B2.GE40023 ordered domain 16 550
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|216594.6.peg.3957
RefSeq: WP_012395312.1
UniProt: B2HM94

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MPVTSAQPPR PGPVAGFIPY PPERVASYRA AGYWTGRSIG SLLRQAAARW PDRVGVVDAG ASHTFCELDK LADRAAAGFV SLGIAPGDRV LLQLPNSCRF AVALFGLLRA GAIPVMCLPG HRLAELHHFA EVSGAVGLIV AQHAGGFDYR SMAERLVAAH PQLRHVIIDG ESGPFLPWSS LSEGATPDID VDTTSPALLL VSGGTTGTPK LIPRTHDDYV YNATASAQLC RLTADDVYLV SLPAAHNFPL ACPGILGSMA VGATIVFSTD PSPEAAFTLI ERHGVTVTAL VPALAKLWTQ ACDWEPVTPK SLRLLQVGGS RLEPEDARQA REALTPGLQQ VFGMAEGLLN FTRLEDPPDL LEHTQGRPLC SADELRIVDG AGAPVAPGAE GELLVRGPYT INGYFCADRD NERCFDPAGF YRSGDLVRLR DDGYLQVTGR VKDVICRCGE TISAQDLEEQ LLSHPAIRSA AAVPLPDPDM GELICAAIVF AGPPQTPQTL SGLNDYLEQR GVAAHIRIDR LVAMPSLPTT PVGKIDKKAI ASQILTTTAT
NT Sequence
gtgcctgtta catccgccca gccgcctcga ccggggccgg tggccgggtt cattccgtac ccacccgaac gagtggccag ctaccgcgcc gccggttatt ggaccggtcg cagcatcggc tcgctgttgc ggcaagcggc cgcacgctgg cctgaccgag tgggtgtggt cgacgccggc gccagccata cgttctgcga actggacaaa ctggccgatc gagccgccgc gggcttcgtt tcgctgggca tcgcaccggg cgaccgggtg ctgctgcagt tacccaattc gtgccggttc gcggtcgcct tgttcggcct gttgcgcgcc ggagccatcc cggtgatgtg cctgcccgga caccggctgg cagaactgca ccacttcgcc gaggtcagcg gtgcggtggg tttgatcgtc gcgcagcacg ccggcggctt cgactaccgg tcgatggccg agcggttggt cgccgctcac ccccagttgc gccacgtcat catcgacggc gagtccgggc cctttctgcc gtggtcgtcg ctgtccgaag gcgccacacc cgacatcgat gtcgacacca catcgcccgc cttgttactc gtctcaggtg gcaccaccgg cactccgaaa ctgatccccc gaacacacga cgactacgtc tacaacgcca ccgcgagtgc gcagttgtgc cggctgaccg ctgacgacgt gtacctcgtg tcgctgccgg cggcgcacaa cttcccgttg gcatgtccgg gcatactcgg ctcgatggcc gtcggcgcca ccatcgtttt cagcaccgac cccagcccgg aggccgcctt caccttgatc gagcgccacg gcgtcaccgt cacggcgctg gtacccgcgc tggccaaact gtggacccag gcctgtgact gggagccggt aaccccgaag tctttgcggc tattgcaagt tggtggctcc agattggagc ccgaggatgc tcgccaggca cgagaggcat tgaccccggg gctgcaacag gtcttcggga tggccgaagg actgctgaac ttcacccgcc tcgaagatcc gccggacctc ctcgagcaca cccagggccg gcccctgtgc tccgctgacg aattgcgcat tgtcgacggc gcgggcgcgc cggtggctcc cggcgcagag ggcgagctac tggtgcgcgg tccctacacc atcaacggct acttctgcgc cgaccgagac aacgagcgct gcttcgatcc agccggcttc tatcgcagcg gcgacttggt ccgactccgc gacgacggct acctgcaggt caccgggcgc gtcaaagacg tcatctgccg ctgcggtgag accatctcgg cccaggacct cgaagaacag ttgctcagcc acccggcgat ccgctcggcc gcggcggtgc cactgccgga cccggatatg ggcgagctga tctgcgctgc aattgttttc gcggggccac cgcagacgcc gcagaccttg tcgggactca atgactatct cgagcagcgt ggcgttgccg cacatatccg catcgatcgg ctggtcgcga tgccgtcgct gccgaccaca ccggtgggaa agatcgacaa gaaggcgatc gcgagccaga tcctgacaac cacggcgacc tga
Details for MymaA.00629.f.B2.GE40023
HARVESTED ON: 3/24/2016
SEQUENCED ON: 3/25/2016
EXPECTED MW: 58kDa
OBSERVED MW: 58kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY: 100
PERCENT COVERAGE: 98
Validated AA Sequence
MAHHHHHHMG FIPYPPERVA SYRAAGYWTG RSIGSLLRQA AARWPDRVGV VDAGASHTFC ELDKLADRAA AGFVSLGIAP GDRVLLQLPN SCRFAVALFG LLRAGAIPVM CLPGHRLAEL HHFAEVSGAV GLIVAQHAGG FDYRSMAERL VAAHPQLRHV IIDGESGPFL PWSSLSEGAT PDIDVDTTSP ALLLVSGGTT GTPKLIPRTH DDYVYNATAS AQLCRLTADD VYLVSLPAAH NFPLACPGIL GSMAVGATIV FSTDPSPEAA FTLIERHGVT VTALVPALAK LWTQACDWEP VTPKSLRLLQ VGGSRLEPED ARQAREALTP GLQQVFGMAE GLLNFTRLED PPDLLEHTQG RPLCSADELR IVDGAGAPVA PGAEGELLVR GPYTINGYFC ADRDNERCFD PAGFYRSGDL VRLRDDGYLQ VTGRVKDVIC RCGETISAQD LEEQLLSHPA IRSAAAVPLP DPDMGELICA AIVFAGPPQT PQTLSGLNDY LEQRGVAAHI RIDRLVAMPS LPTTPVGKID KKAIA
Validated NT Sequence
ctcgcgatcg ccttcttgtc gatctttccc accggtgtgg tcggcagcga cggcatcgcg accagccgat cgatgcggat atgtgcggca acgccacgct gctcgagata gtcattgagt cccgacaagg tctgcggcgt ctgcggtggc cccgcgaaaa caattgcagc gcagatcagc tcgcccatat ccgggtccgg cagtggcacc gccgcggccg agcggatcgc cgggtggctg agcaactgtt cttcgaggtc ctgggccgag atggtctcac cgcagcggca gatgacgtct ttgacgcgcc cggtgacctg caggtagccg tcgtcgcgga gtcggaccaa gtcgccgctg cgatagaagc cggctggatc gaagcagcgc tcgttgtctc ggtcggcgca gaagtagccg ttgatggtgt agggaccgcg caccagtagc tcgccctctg cgccgggagc caccggcgcg cccgcgccgt cgacaatgcg caattcgtca gcggagcaca ggggccggcc ctgggtgtgc tcgaggaggt ccggcggatc ttcgaggcgg gtgaagttca gcagtccttc ggccatcccg aagacctgtt gcagccccgg ggtcaatgcc tctcgtgcct ggcgagcatc ctcgggctcc aatctggagc caccaacttg caatagccgc aaagacttcg gggttaccgg ctcccagtca caggcctggg tccacagttt ggccagcgcg ggtaccagcg ccgtgacggt gacgccgtgg cgctcgatca aggtgaaggc ggcctccggg ctggggtcgg tgctgaaaac gatggtggcg ccgacggcca tcgagccgag tatgcccgga catgccaacg ggaagttgtg cgccgccggc agcgacacga ggtacacgtc gtcagcggtc agccggcaca actgcgcact cgcggtggcg ttgtagacgt agtcgtcgtg tgttcggggg atcagtttcg gagtgccggt ggtgccacct gagacgagta acaaggcggg cgatgtggtg tcgacatcga tgtcgggtgt ggcgccttcg gacagcgacg accacggcag aaagggcccg gactcgccgt cgatgatgac gtggcgcaac tgggggtgag cggcgaccaa ccgctcggcc atcgaccggt agtcgaagcc gccggcgtgc tgcgcgacga tcaaacccac cgcaccgctg acctcggcga agtggtgcag ttctgccagc cggtgtccgg gcaggcacat caccgggatg gctccggcgc gcaacaggcc gaacaaggcg accgcgaacc ggcacgaatt gggtaactgc agcagcaccc ggtcgcccgg tgcgatgccc agcgaaacga agcccgcggc ggctcgatcg gccagtttgt ccagttcgca gaacgtatgg ctggcgccgg cgtcgaccac acccactcgg tcaggccagc gtgcggccgc ttgccgcaac agcgagccga tgctgcgacc ggtccaataa ccggcggcgc ggtagctggc cactcgttcg ggtgggtacg gaatgaaccc catatggtgg tggtggtggt gagccat
Expected Protein Sequence
MAHHHHHHGF IPYPPERVAS YRAAGYWTGR SIGSLLRQAA ARWPDRVGVV DAGASHTFCE LDKLADRAAA GFVSLGIAPG DRVLLQLPNS CRFAVALFGL LRAGAIPVMC LPGHRLAELH HFAEVSGAVG LIVAQHAGGF DYRSMAERLV AAHPQLRHVI IDGESGPFLP WSSLSEGATP DIDVDTTSPA LLLVSGGTTG TPKLIPRTHD DYVYNATASA QLCRLTADDV YLVSLPAAHN FPLACPGILG SMAVGATIVF STDPSPEAAF TLIERHGVTV TALVPALAKL WTQACDWEPV TPKSLRLLQV GGSRLEPEDA RQAREALTPG LQQVFGMAEG LLNFTRLEDP PDLLEHTQGR PLCSADELRI VDGAGAPVAP GAEGELLVRG PYTINGYFCA DRDNERCFDP AGFYRSGDLV RLRDDGYLQV TGRVKDVICR CGETISAQDL EEQLLSHPAI RSAAAVPLPD PDMGELICAA IVFAGPPQTP QTLSGLNDYL EQRGVAAHIR IDRLVAMPSL PTTPVGKIDK KAIASQILTT TAT
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgggttca ttccgtaccc acccgaacga gtggccagct accgcgccgc cggttattgg accggtcgca gcatcggctc gctgttgcgg caagcggccg cacgctggcc tgaccgagtg ggtgtggtcg acgccggcgc cagccatacg ttctgcgaac tggacaaact ggccgatcga gccgccgcgg gcttcgtttc gctgggcatc gcaccgggcg accgggtgct gctgcagtta cccaattcgt gccggttcgc ggtcgccttg ttcggcctgt tgcgcgccgg agccatcccg gtgatgtgcc tgcccggaca ccggctggca gaactgcacc acttcgccga ggtcagcggt gcggtgggtt tgatcgtcgc gcagcacgcc ggcggcttcg actaccggtc gatggccgag cggttggtcg ccgctcaccc ccagttgcgc cacgtcatca tcgacggcga gtccgggccc tttctgccgt ggtcgtcgct gtccgaaggc gccacacccg acatcgatgt cgacaccaca tcgcccgcct tgttactcgt ctcaggtggc accaccggca ctccgaaact gatcccccga acacacgacg actacgtcta caacgccacc gcgagtgcgc agttgtgccg gctgaccgct gacgacgtgt acctcgtgtc gctgccggcg gcgcacaact tcccgttggc atgtccgggc atactcggct cgatggccgt cggcgccacc atcgttttca gcaccgaccc cagcccggag gccgccttca ccttgatcga gcgccacggc gtcaccgtca cggcgctggt acccgcgctg gccaaactgt ggacccaggc ctgtgactgg gagccggtaa ccccgaagtc tttgcggcta ttgcaagttg gtggctccag attggagccc gaggatgctc gccaggcacg agaggcattg accccggggc tgcaacaggt cttcgggatg gccgaaggac tgctgaactt cacccgcctc gaagatccgc cggacctcct cgagcacacc cagggccggc ccctgtgctc cgctgacgaa ttgcgcattg tcgacggcgc gggcgcgccg gtggctcccg gcgcagaggg cgagctactg gtgcgcggtc cctacaccat caacggctac ttctgcgccg accgagacaa cgagcgctgc ttcgatccag ccggcttcta tcgcagcggc gacttggtcc gactccgcga cgacggctac ctgcaggtca ccgggcgcgt caaagacgtc atctgccgct gcggtgagac catctcggcc caggacctcg aagaacagtt gctcagccac ccggcgatcc gctcggccgc ggcggtgcca ctgccggacc cggatatggg cgagctgatc tgcgctgcaa ttgttttcgc ggggccaccg cagacgccgc agaccttgtc gggactcaat gactatctcg agcagcgtgg cgttgccgca catatccgca tcgatcggct ggtcgcgatg ccgtcgctgc cgaccacacc ggtgggaaag atcgacaaga aggcgatcgc gagccagatc ctgacaacca cggcgacctg agtaagatag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa agctcatcag cgtggtcgtg aagcgattca cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg gcgggaccag agaaaaatca ctcagggtca atgccagcgc ttcgttaata cagatgtagg tgttccacag ggtagccagc agcatcctgc gatgcagatc cggaacataa tggtgcaggg cgctgacttc cgcgtttcca gactttacga aacacggaaa ccgaagacca ttcatgttgt tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga ttcattctgc taaccagtaa ggcaaccccg ccagcctagc cgggtcctca acgacaggag cacgatcatg cgcacccgtg gccaggaccc aacgctgccc gagatgcgcc gcgtgcggct gctggagatg gcggacgcga tggatatgtt ctgccaaggg ttggtttgcg cattcacagt tctccgcaag aattgattgg ctccaattct tggagtggtg aatccgttag cgaggtgccg ccggcttcca ttcaggtcga ggtggcccgg ctccatgcac cgcgacgcaa cgcggggagg cagacaaggt atagggcggc gcctacaatc catgccaacc cgttccatgt gctcgccgag gcggcataaa tcgccgtgac gatcagcggt ccagtgatcg aagttaggct ggtaagagcc gcgagcgatc cttgaagctg tccctgatgg tcgtcatcta cctgcctgga cagcatggcc tgcaacgcgg gcatcccgat gccgccggaa gcgagaagaa tcataatggg gaaggccatc cagcctcgcg tcgcgaacgc cagcaagacg tagcccagcg cgtcggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aat