MymaA.00620.a

Putative uncharacterized protein

CENTER ID: MymaA.00620.a
ORGANISM: Mycobacterium marinum ATCC BAA-535 / M
ASSOCIATED DISEASE: Cutaneous ulcerations, deep abscesses
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MymaA.00620.a.A1.GE30049 Full length( MymaA.00620.a ) 1 255
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MymaA.00620.a.A1.PW29921 Full length( MymaA.00620.a ) 1 255

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|216594.6.peg.1662
RefSeq: YP_001849869.1
UniProt: B2HGM8

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTRDLPAQRT ASRIYDLHSG GSLLDADESA RVTGLLEEAG FVAAGPRPAW RARTDGRPTI CVYCASGGDN PADLSLAAAV GTEIGRRGWR LISGGGTIAM MGAVARAART AGAHTTGVIP RMLLQSELAD LGADELIVTD TLTQRKHLMG ERADGFLTLP GGLGTLEELL ECWTAASLGI HHKPVVLLDP AGHYRGLLDW LEELRQQGFV RTAAIDRLIV TTDVDSAFSN LCPSTRSAFA SSGEASVSER THAHY
NT Sequence
GTGACTCGAG ACCTGCCGGC ACAACGCACG GCCAGCCGAA TCTACGACCT GCACAGCGGC GGCTCCCTGC TGGACGCCGA CGAATCCGCG CGGGTGACCG GATTGCTCGA GGAGGCGGGA TTCGTTGCGG CTGGGCCCAG ACCTGCCTGG CGCGCTCGGA CCGATGGCCG TCCGACGATT TGCGTCTACT GCGCTTCCGG CGGCGACAAC CCCGCCGACC TGAGCCTGGC CGCCGCCGTG GGCACCGAAA TCGGTAGACG CGGATGGCGA TTGATCTCCG GAGGCGGCAC CATCGCCATG ATGGGCGCGG TGGCCCGCGC GGCCAGAACC GCCGGAGCCC ACACCACAGG CGTAATCCCG AGAATGCTCC TGCAATCCGA ACTTGCTGAC CTAGGTGCCG ATGAGCTGAT CGTCACCGAC ACACTCACCC AACGCAAACA CCTCATGGGC GAACGCGCTG ACGGGTTCCT GACGCTGCCC GGCGGTCTGG GCACCCTCGA AGAGCTGCTC GAATGCTGGA CCGCGGCCAG CCTCGGCATA CATCACAAGC CCGTAGTCCT GCTGGACCCC GCCGGACACT ACCGCGGACT GCTCGACTGG CTCGAAGAAC TGCGGCAGCA AGGCTTCGTG CGCACCGCCG CGATCGATCG GCTCATCGTC ACGACCGACG TCGACAGCGC ATTCAGCAAC CTCTGCCCAT CAACTCGGTC GGCGTTCGCG TCGTCTGGGG AGGCCAGCGT CTCAGAGCGG ACCCATGCGC ATTAT
Details for MymaA.00620.a.A1.GE30049
HARVESTED ON: 8/10/2010
SEQUENCED ON: 8/16/2010
EXPECTED MW: 29kDa
OBSERVED MW: 29kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 99
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMVTRDLPAQ RTASRIYDLH SGGSLLDADE SARVTGLLEE AGFVAAGPRP AWRARTDGRP TICVYCASGG DNPADLSLAA AVGTEIGRRG WRLISGGGTI AMMGAVARAA RTAGAHTTGV IPRMLLQSEL ADLGADELIV TDTLTQRKHL MGERADGFLT LPGGLGTLEE LLECWTAASL GIHHKPVVLL DPAGHYRGLL DWLEELRQQG FVRTAAIDRL IVTTDVXSAF SNLCPSTRSA FASSGEASVS ERTHAH
Validated NT Sequence
ttattaatgc gcatgggtcc gctctgagac gctggcctcc ccagacgacg cgaacgccga ccgagttgat gggcagaggt tgctgaatgc gctgtngacg tcggtcgtga cgatgagccg atcgatcgcg gcggtgcgca cgaagccttg ctgccgcagt tcttcgagcc agtcgagcag tccgcggtag tgtccggcgg ggtccagcag gactacgggc ttgtgatgta tgccgaggct ggccgcggtc cagcattcga gcagctcttc gagggtgccc agaccgccgg gcagcgtcag gaacccgtca gcgcgttcgc ccatgaggtg tttgcgttgg gtgagtgtgt cggtgacgat cagctcatcg gcacctaggt cagcaagttc ggattgcagg agcattctcg ggattacgcc tgtggtgtgg gctccggcgg ttctggccgc gcgggccacc gcgcccatca tggcgatggt gccgcctccg gagatcaatc gccatccgcg tctaccgatt tcggtgccca cggcggcggc caggctcagg tcggcggggt tgtcgccgcc ggaagcgcag tagacgcaaa tcgtcggacg gccatcggtc cgagcgcgcc aggcaggtct gggcccagcc gcaacgaatc ccgcctcctc gagcaatccg gtcacccgcg cggattcgtc ggcgtccagc agggagccgc cgctgtgcag gtcgtagatt cggctggccg tgcgttgtgc cggcaggtct cgagtcacca tcgaaccagg accctgggtc tgagcttcca gggtacccat atgatggtga tggtgatgag ccat
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVTRDLPAQ RTASRIYDLH SGGSLLDADE SARVTGLLEE AGFVAAGPRP AWRARTDGRP TICVYCASGG DNPADLSLAA AVGTEIGRRG WRLISGGGTI AMMGAVARAA RTAGAHTTGV IPRMLLQSEL ADLGADELIV TDTLTQRKHL MGERADGFLT LPGGLGTLEE LLECWTAASL GIHHKPVVLL DPAGHYRGLL DWLEELRQQG FVRTAAIDRL IVTTDVDSAF SNLCPSTRSA FASSGEASVS ERTHAH
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtgactcga gacctgccgg cacaacgcac ggccagccga atctacgacc tgcacagcgg cggctccctg ctggacgccg acgaatccgc gcgggtgacc ggattgctcg aggaggcggg attcgttgcg gctgggccca gacctgcctg gcgcgctcgg accgatggcc gtccgacgat ttgcgtctac tgcgcttccg gcggcgacaa ccccgccgac ctgagcctgg ccgccgccgt gggcaccgaa atcggtagac gcggatggcg attgatctcc ggaggcggca ccatcgccat gatgggcgcg gtggcccgcg cggccagaac cgccggagcc cacaccacag gcgtaatccc gagaatgctc ctgcaatccg aacttgctga cctaggtgcc gatgagctga tcgtcaccga cacactcacc caacgcaaac acctcatggg cgaacgcgct gacgggttcc tgacgctgcc cggcggtctg ggcaccctcg aagagctgct cgaatgctgg accgcggcca gcctcggcat acatcacaag cccgtagtcc tgctggaccc cgccggacac taccgcggac tgctcgactg gctcgaagaa ctgcggcagc aaggcttcgt gcgcaccgcc gcgatcgatc ggctcatcgt cacgaccgac gtcgacagcg cattcagcaa cctctgccca tcaactcggt cggcgttcgc gtcgtctggg gaggccagcg tctcagagcg gacccatgcg cataaacagc acgaacaagt tctgcagcca agcttctcga ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaa
Details for MymaA.00620.a.A1.PW29921
PURIFICATION DATe: 10/18/2010
CONCENTRATION: 28.36mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 10
VIAL VOLUME: 200µl
PERCENT IDENTITY: 99
PERCENT COVERAGE: 100
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMVTRDLPAQ RTASRIYDLH SGGSLLDADE SARVTGLLEE AGFVAAGPRP AWRARTDGRP TICVYCASGG DNPADLSLAA AVGTEIGRRG WRLISGGGTI AMMGAVARAA RTAGAHTTGV IPRMLLQSEL ADLGADELIV TDTLTQRKHL MGERADGFLT LPGGLGTLEE LLECWTAASL GIHHKPVVLL DPAGHYRGLL DWLEELRQQG FVRTAAIDRL IVTTDVXSAF SNLCPSTRSA FASSGEASVS ERTHAH
Validated NT Sequence
ttattaatgc gcatgggtcc gctctgagac gctggcctcc ccagacgacg cgaacgccga ccgagttgat gggcagaggt tgctgaatgc gctgtngacg tcggtcgtga cgatgagccg atcgatcgcg gcggtgcgca cgaagccttg ctgccgcagt tcttcgagcc agtcgagcag tccgcggtag tgtccggcgg ggtccagcag gactacgggc ttgtgatgta tgccgaggct ggccgcggtc cagcattcga gcagctcttc gagggtgccc agaccgccgg gcagcgtcag gaacccgtca gcgcgttcgc ccatgaggtg tttgcgttgg gtgagtgtgt cggtgacgat cagctcatcg gcacctaggt cagcaagttc ggattgcagg agcattctcg ggattacgcc tgtggtgtgg gctccggcgg ttctggccgc gcgggccacc gcgcccatca tggcgatggt gccgcctccg gagatcaatc gccatccgcg tctaccgatt tcggtgccca cggcggcggc caggctcagg tcggcggggt tgtcgccgcc ggaagcgcag tagacgcaaa tcgtcggacg gccatcggtc cgagcgcgcc aggcaggtct gggcccagcc gcaacgaatc ccgcctcctc gagcaatccg gtcacccgcg cggattcgtc ggcgtccagc agggagccgc cgctgtgcag gtcgtagatt cggctggccg tgcgttgtgc cggcaggtct cgagtcacca tcgaaccagg accctgggtc tgagcttcca gggtacccat atgatggtga tggtgatgag ccat
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVTRDLPAQ RTASRIYDLH SGGSLLDADE SARVTGLLEE AGFVAAGPRP AWRARTDGRP TICVYCASGG DNPADLSLAA AVGTEIGRRG WRLISGGGTI AMMGAVARAA RTAGAHTTGV IPRMLLQSEL ADLGADELIV TDTLTQRKHL MGERADGFLT LPGGLGTLEE LLECWTAASL GIHHKPVVLL DPAGHYRGLL DWLEELRQQG FVRTAAIDRL IVTTDVDSAF SNLCPSTRSA FASSGEASVS ERTHAH
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGTGACTCGA GACCTGCCGG CACAACGCAC GGCCAGCCGA ATCTACGACC TGCACAGCGG CGGCTCCCTG CTGGACGCCG ACGAATCCGC GCGGGTGACC GGATTGCTCG AGGAGGCGGG ATTCGTTGCG GCTGGGCCCA GACCTGCCTG GCGCGCTCGG ACCGATGGCC GTCCGACGAT TTGCGTCTAC TGCGCTTCCG GCGGCGACAA CCCCGCCGAC CTGAGCCTGG CCGCCGCCGT GGGCACCGAA ATCGGTAGAC GCGGATGGCG ATTGATCTCC GGAGGCGGCA CCATCGCCAT GATGGGCGCG GTGGCCCGCG CGGCCAGAAC CGCCGGAGCC CACACCACAG GCGTAATCCC GAGAATGCTC CTGCAATCCG AACTTGCTGA CCTAGGTGCC GATGAGCTGA TCGTCACCGA CACACTCACC CAACGCAAAC ACCTCATGGG CGAACGCGCT GACGGGTTCC TGACGCTGCC CGGCGGTCTG GGCACCCTCG AAGAGCTGCT CGAATGCTGG ACCGCGGCCA GCCTCGGCAT ACATCACAAG CCCGTAGTCC TGCTGGACCC CGCCGGACAC TACCGCGGAC TGCTCGACTG GCTCGAAGAA CTGCGGCAGC AAGGCTTCGT GCGCACCGCC GCGATCGATC GGCTCATCGT CACGACCGAC GTCGACAGCG CATTCAGCAA CCTCTGCCCA TCAACTCGGT CGGCGTTCGC GTCGTCTGGG GAGGCCAGCG TCTCAGAGCG GACCCATGCG CATAAACAGC ACGAACAAGT TCTGCAGCCA AGCTTCTCGA GGATCCGGCT GCTAACAAAG CCCGAAAGGA AGCTGAGTTG GCTGCTGCCA CCGCTGAGCA ATAACTAGCA TAACCCCTTG GGGCCTCTAA ACGGGTCTTG AGGGGTTTTT TGCTGAAAGG AGGAACTATA TCCGGATATC CACAGGACGG GTGTGGTCGC CATGATCGCG TAGTCGATAG TGGCTCCAAG TAGCGAAGCG AGCAGGACTG GGCGGCGGCC AAAGCGGTCG GACAGTGCTC CGAGAACGGG TGCGCATAGA AATTGCATCA ACGCATATAG CGCTAGCAGC ACGCCATAGT GACTGGCGAT GCTGTCGGAA TGGACGATAT CCCGCAAGAG GCCCGGCAGT ACCGGCATAA CCAAGCCTAT GCCTACAGCA TCCAGGGTGA CGGTGCCGAG GATGACGATG AGCGCATTGT TAGATTTCAT ACACGGTGCC TGACTGCGTT AGCAATTTAA CTGTGATAAA CTACCGCATT AAAGCTTATC GATGATAAGC TGTCAAACAT GAGAA