MymaA.00587.e

Two-component regulator-receiver domain

CENTER ID: MymaA.00587.e
ORGANISM: Mycobacterium marinum ATCC BAA-535 / M
ASSOCIATED DISEASE: Cutaneous ulcerations, deep abscesses
CURRENT STATUS: purified
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MymaA.00587.e.A1.GE30046 Full length( MymaA.00587.e ) 1 247
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MymaA.00587.e.A1.PS00865 Full length( MymaA.00587.e ) 1 247

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|216594.6.peg.2453
RefSeq: YP_001850602.1
UniProt: B2HPE3

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTDRSRTDQT GSRQVASRTD RFSVGAPRVL VVEDSETIRE MVNEALADVG YHTDTRSDGE GLERVLQGLR PDLVVLDVML PGRDGFALID VIREWGDIGI VLITARDGLP DRLRGLDGGA DDYVVKPFEL AELVSRVGAV LRRRGRLPRV VQVGDLMLDS EAGVAARDGH QLDLTATELR LLDFLVQQRG RIVSATQILN AVWGYDAYDA NLVQVHVSSL RRKLEAHGPR IVQTVRGIGY RLQPPRS
NT Sequence
ATGACCGATC GTTCGCGAAC CGACCAGACC GGCAGTCGCC AGGTTGCGAG CCGCACGGAC CGATTCAGTG TCGGCGCGCC GAGAGTGCTG GTGGTCGAGG ATTCCGAGAC CATCCGCGAA ATGGTCAACG AGGCCCTGGC CGACGTCGGC TACCACACCG ACACCCGCTC CGATGGGGAG GGACTGGAGA GAGTTTTGCA GGGCCTACGG CCCGACCTGG TGGTACTCGA CGTGATGCTG CCGGGGCGGG ACGGATTTGC CCTCATCGAC GTCATCCGCG AGTGGGGCGA TATCGGCATC GTCTTGATCA CCGCCCGCGA CGGTCTGCCC GACCGGCTGC GCGGTCTCGA CGGAGGTGCC GACGACTATG TGGTCAAACC GTTCGAGCTC GCCGAACTCG TGTCTCGGGT GGGCGCGGTC CTGCGCCGCC GGGGTCGGCT GCCCCGGGTG GTTCAGGTCG GCGATCTGAT GCTGGACAGC GAGGCCGGGG TCGCTGCGCG CGACGGCCAC CAACTGGACC TGACCGCCAC CGAACTCCGA CTGCTCGACT TCCTGGTACA ACAACGCGGC CGCATCGTGA GCGCCACGCA GATCCTCAAT GCCGTGTGGG GCTACGACGC CTACGACGCC AACCTGGTGC AGGTGCATGT CAGCAGCTTG CGCCGCAAGC TCGAAGCGCA TGGCCCACGA ATTGTGCAGA CCGTCCGAGG CATCGGCTAC CGGTTGCAGC CGCCGAGGTC A
Details for MymaA.00587.e.A1.GE30046
HARVESTED ON: 8/10/2010
SEQUENCED ON: 8/16/2010
EXPECTED MW: 29kDa
OBSERVED MW: 29kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMTDRSRTDQ TGSRQVASRT DRFSVGAPRV LVVEDSETIR EMVNEALADV GYHTDTRSDG EGLERVLQGL RPDLVVLDVM LPGRDGFALI DVIREWGDIG IVLITARDGL PDRLRGLDGG ADDYVVKPFE LAELVSRVGA VLRRRGRLPR VVQVGDLMLD SEAGVAARDG HQLDLTATEL RLLDFLVQQR GRIVSATQIL NAVWGYDAYD ANLVQVHVSS LRRKLEAHGP RIVQTVRGIG YRLQPPR
Validated NT Sequence
ttattacctc ggcggctgca accggtagcc gatgcctcgg acggtctgca caattcgtgg gccatgcgct tcgagcttgc ggcgcaagct gctgacatgc acctgcacca ggttggcgtc gtaggcgtcg tagccccaca cggcattgag gatctgcgtg gcgctcacga tgcggccgcg ttgttgtacc aggaagtcga gcagtcggag ttcggtggcg gtcaggtcca gttggtggcc gtcgcgcgca gcgaccccgg cctcgctgtc cagcatcaga tcgccgacct gaaccacccg gggcagccga ccccggcggc gcaggaccgc gcccacccga gacacgagtt cggcgagctc gaacggtttg accacatagt cgtcggcacc tccgtcgaga ccgcgcagcc ggtcgggcag accgtcgcgg gcggtgatca agacgatgcc gatatcgccc cactcgcgga tgacgtcgat gagggcaaat ccgtcccgcc ccggcagcat cacgtcgagt accaccaggt cgggccgtag gccctgcaaa actctctcca gtccctcccc atcggagcgg gtgtcggtgt ggtagccgac gtcggccagg gcctcgttga ccatttcgcg gatggtctcg gaatcctcga ccaccagcac tctcggcgcg ccgacactga atcggtccgt gcggctcgca acctggcgac tgccggtctg gtcggttcgc gaacgatcgg tcatcgaacc aggaccctgg gtctgagctt ccagggtacc catatgatgg tgatggtgat gagccat
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMTDRSRTDQ TGSRQVASRT DRFSVGAPRV LVVEDSETIR EMVNEALADV GYHTDTRSDG EGLERVLQGL RPDLVVLDVM LPGRDGFALI DVIREWGDIG IVLITARDGL PDRLRGLDGG ADDYVVKPFE LAELVSRVGA VLRRRGRLPR VVQVGDLMLD SEAGVAARDG HQLDLTATEL RLLDFLVQQR GRIVSATQIL NAVWGYDAYD ANLVQVHVSS LRRKLEAHGP RIVQTVRGIG YRLQPPR
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gaccgatcgt tcgcgaaccg accagaccgg cagtcgccag gttgcgagcc gcacggaccg attcagtgtc ggcgcgccga gagtgctggt ggtcgaggat tccgagacca tccgcgaaat ggtcaacgag gccctggccg acgtcggcta ccacaccgac acccgctccg atggggaggg actggagaga gttttgcagg gcctacggcc cgacctggtg gtactcgacg tgatgctgcc ggggcgggac ggatttgccc tcatcgacgt catccgcgag tggggcgata tcggcatcgt cttgatcacc gcccgcgacg gtctgcccga ccggctgcgc ggtctcgacg gaggtgccga cgactatgtg gtcaaaccgt tcgagctcgc cgaactcgtg tctcgggtgg gcgcggtcct gcgccgccgg ggtcggctgc cccgggtggt tcaggtcggc gatctgatgc tggacagcga ggccggggtc gctgcgcgcg acggccacca actggacctg accgccaccg aactccgact gctcgacttc ctggtacaac aacgcggccg catcgtgagc gccacgcaga tcctcaatgc cgtgtggggc tacgacgcct acgacgccaa cctggtgcag gtgcatgtca gcagcttgcg ccgcaagctc gaagcgcatg gcccacgaat tgtgcagacc gtccgaggca tcggctaccg gttgcagccg ccgaggaaac agcacgaaca agttctgcag ccaagcttct cgaggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaa
Details for MymaA.00587.e.A1.PS00865
PURIFICATION DATe: 11/10/2010
CONCENTRATION: 10mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 4
VIAL VOLUME: 200µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMTDRSRTDQ TGSRQVASRT DRFSVGAPRV LVVEDSETIR EMVNEALADV GYHTDTRSDG EGLERVLQGL RPDLVVLDVM LPGRDGFALI DVIREWGDIG IVLITARDGL PDRLRGLDGG ADDYVVKPFE LAELVSRVGA VLRRRGRLPR VVQVGDLMLD SEAGVAARDG HQLDLTATEL RLLDFLVQQR GRIVSATQIL NAVWGYDAYD ANLVQVHVSS LRRKLEAHGP RIVQTVRGIG YRLQPPR
Validated NT Sequence
ttattacctc ggcggctgca accggtagcc gatgcctcgg acggtctgca caattcgtgg gccatgcgct tcgagcttgc ggcgcaagct gctgacatgc acctgcacca ggttggcgtc gtaggcgtcg tagccccaca cggcattgag gatctgcgtg gcgctcacga tgcggccgcg ttgttgtacc aggaagtcga gcagtcggag ttcggtggcg gtcaggtcca gttggtggcc gtcgcgcgca gcgaccccgg cctcgctgtc cagcatcaga tcgccgacct gaaccacccg gggcagccga ccccggcggc gcaggaccgc gcccacccga gacacgagtt cggcgagctc gaacggtttg accacatagt cgtcggcacc tccgtcgaga ccgcgcagcc ggtcgggcag accgtcgcgg gcggtgatca agacgatgcc gatatcgccc cactcgcgga tgacgtcgat gagggcaaat ccgtcccgcc ccggcagcat cacgtcgagt accaccaggt cgggccgtag gccctgcaaa actctctcca gtccctcccc atcggagcgg gtgtcggtgt ggtagccgac gtcggccagg gcctcgttga ccatttcgcg gatggtctcg gaatcctcga ccaccagcac tctcggcgcg ccgacactga atcggtccgt gcggctcgca acctggcgac tgccggtctg gtcggttcgc gaacgatcgg tcatcgaacc aggaccctgg gtctgagctt ccagggtacc catatgatgg tgatggtgat gagccat
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMTDRSRTDQ TGSRQVASRT DRFSVGAPRV LVVEDSETIR EMVNEALADV GYHTDTRSDG EGLERVLQGL RPDLVVLDVM LPGRDGFALI DVIREWGDIG IVLITARDGL PDRLRGLDGG ADDYVVKPFE LAELVSRVGA VLRRRGRLPR VVQVGDLMLD SEAGVAARDG HQLDLTATEL RLLDFLVQQR GRIVSATQIL NAVWGYDAYD ANLVQVHVSS LRRKLEAHGP RIVQTVRGIG YRLQPPR
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GACCGATCGT TCGCGAACCG ACCAGACCGG CAGTCGCCAG GTTGCGAGCC GCACGGACCG ATTCAGTGTC GGCGCGCCGA GAGTGCTGGT GGTCGAGGAT TCCGAGACCA TCCGCGAAAT GGTCAACGAG GCCCTGGCCG ACGTCGGCTA CCACACCGAC ACCCGCTCCG ATGGGGAGGG ACTGGAGAGA GTTTTGCAGG GCCTACGGCC CGACCTGGTG GTACTCGACG TGATGCTGCC GGGGCGGGAC GGATTTGCCC TCATCGACGT CATCCGCGAG TGGGGCGATA TCGGCATCGT CTTGATCACC GCCCGCGACG GTCTGCCCGA CCGGCTGCGC GGTCTCGACG GAGGTGCCGA CGACTATGTG GTCAAACCGT TCGAGCTCGC CGAACTCGTG TCTCGGGTGG GCGCGGTCCT GCGCCGCCGG GGTCGGCTGC CCCGGGTGGT TCAGGTCGGC GATCTGATGC TGGACAGCGA GGCCGGGGTC GCTGCGCGCG ACGGCCACCA ACTGGACCTG ACCGCCACCG AACTCCGACT GCTCGACTTC CTGGTACAAC AACGCGGCCG CATCGTGAGC GCCACGCAGA TCCTCAATGC CGTGTGGGGC TACGACGCCT ACGACGCCAA CCTGGTGCAG GTGCATGTCA GCAGCTTGCG CCGCAAGCTC GAAGCGCATG GCCCACGAAT TGTGCAGACC GTCCGAGGCA TCGGCTACCG GTTGCAGCCG CCGAGGAAAC AGCACGAACA AGTTCTGCAG CCAAGCTTCT CGAGGATCCG GCTGCTAACA AAGCCCGAAA GGAAGCTGAG TTGGCTGCTG CCACCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC TAAACGGGTC TTGAGGGGTT TTTTGCTGAA AGGAGGAACT ATATCCGGAT ATCCACAGGA CGGGTGTGGT CGCCATGATC GCGTAGTCGA TAGTGGCTCC AAGTAGCGAA GCGAGCAGGA CTGGGCGGCG GCCAAAGCGG TCGGACAGTG CTCCGAGAAC GGGTGCGCAT AGAAATTGCA TCAACGCATA TAGCGCTAGC AGCACGCCAT AGTGACTGGC GATGCTGTCG GAATGGACGA TATCCCGCAA GAGGCCCGGC AGTACCGGCA TAACCAAGCC TATGCCTACA GCATCCAGGG TGACGGTGCC GAGGATGACG ATGAGCGCAT TGTTAGATTT CATACACGGT GCCTGACTGC GTTAGCAATT TAACTGTGAT AAACTACCGC ATTAAAGCTT ATCGATGATA AGCTGTCAAA CATGAGAA