SSGCID: Target Page

MymaA.00587.c

Two-component system response phosphate regulon transcriptional regulator, PhoP

CENTER ID:	MymaA.00587.c
ORGANISM:	Mycobacterium marinum ATCC BAA-535 / M
ASSOCIATED DISEASE:	Cutaneous ulcerations, deep abscesses
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	False
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MymaA.00587.c.A1.GE30044	Full length( MymaA.00587.c )		1	240

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|216594.6.peg.5295
RefSeq:	YP_001853201.1
UniProt:	B2HHU6

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MTATTPGEAT TPEARILVVD DEDNIVELLS VSLKFQGFEV HTATNGAQAL DRARETRPDA VILDVMMPGM DGFGVLRRLR ADGIDAPALF LTARDSLQDK IAGLTLGGDD YVTKPFSLEE VVARLRVILR RAGKGSAEPR NSRLTFADIE LDEETHEVWK AGQPVSLSPT EFTLLRYFVI NAGTVLSKPK ILDHVWRYDF GGDVNVVESY VSYLRRKIDT GDKRLLHTLR GVGYVLREPR

NT Sequence

ATGACAGCCA CAACCCCGGG CGAGGCCACC ACACCAGAAG CGCGCATCCT CGTTGTCGAC GACGAGGACA ACATCGTCGA GCTGCTCTCG GTCAGTCTGA AGTTTCAGGG ATTCGAGGTG CACACGGCAA CCAACGGCGC GCAGGCGTTG GATCGGGCCA GGGAAACCCG CCCGGACGCG GTGATTCTCG ATGTGATGAT GCCCGGGATG GACGGCTTCG GCGTGCTGCG GCGGCTGCGC GCCGACGGCA TCGATGCACC CGCACTGTTC TTGACCGCCC GGGACTCGCT GCAGGACAAG ATCGCGGGGC TGACCCTGGG CGGCGATGAC TACGTGACAA AGCCCTTCAG CCTGGAAGAG GTGGTTGCCC GGTTGCGGGT AATTCTGCGC CGCGCCGGCA AGGGCAGCGC AGAGCCGCGC AATTCCCGCC TTACGTTCGC CGATATCGAG CTCGATGAGG AAACCCACGA AGTGTGGAAG GCCGGCCAGC CGGTGTCGCT ATCGCCCACC GAGTTCACCT TGTTGCGCTA TTTCGTGATC AATGCGGGCA CGGTGCTGAG CAAGCCGAAA ATTCTCGACC ATGTGTGGCG CTACGACTTC GGTGGCGACG TCAACGTCGT CGAGTCCTAC GTGTCGTATC TGCGGCGCAA GATCGATACC GGCGACAAGC GGCTGCTGCA CACCTTGCGT GGGGTCGGCT ATGTGCTGCG GGAGCCGCGA

Details for MymaA.00587.c.A1.GE30044

HARVESTED ON:	8/10/2010
SEQUENCED ON:	8/16/2010
EXPECTED MW:	28kDa
OBSERVED MW:	34kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Few (1-10)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	99
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SMTATTPGEA TTPEARILVV DDEDNIVELL SVSLKFQGFE VHTATNGAQA LDRARETRPD AVILDVMMPG MDGFGVLRRL RADGIDAPAL FLTARDSLQD KIAGLTLGGD DYVTKPFSLE EVVARLRVIL RRAGKGSAEP RNSRLTFADI ELDEETHEVW KAGQPVSLSP TEFTLLRYFV INAGTVLSKP KILDHVWRYD FGGDVNVVES YVSYLRRKID TGDKRLLHTL RXVGYVLREP

Validated NT Sequence

tttgttnnnt ttaagaagga gatataccat ggctcatcac catcaccatc atatgggtac cctggaagct cagacccagg gtcctggttc gatgacagcc acaaccccgg gcgaggccac cacaccagaa gcgcgcatcc tcgttgtcga cgacgaggac aacatcgtcg agctgctctc ggtcagtctg aagtttcagg gattcgaggt gcacacggca accaacggcg cgcaggcgtt ggatcgggcc agggaaaccc gcccggacgc ggtgattctc gatgtgatga tgcccgggat ggacggcttc ggcgtgctgc ggcggctgcg cgccgacggc atcgatgcac ccgcactgtt cttgaccgcc cgggactcgc tgcaggacaa gatcgcgggg ctgaccctgg gcggcgatga ctacgtgaca aagcccttca gcctggaaga ggtggttgcc cggttgcggg taattctgcg ccgcgccggc aagggcagcg cagagccgcg caattcccgc cttacgttcg ccgatatcga gctcgatgag gaaacccacg aagtgtggaa ggccggccag ccggtgtcgc tatcgcccac cgagttcacc ttgttgcgct atttcgtgat caatgcgggc acggtgctga gcaagccgaa aattctcgac catgtgtggc gctacgactt cggtggcgac gtcaacgtcg tcgagtccta cgtgtcgtat ctgcggcgca agatcgatac cggcgacaag cggctgctgc acaccttgcg tgnggtcggc tatgtgctgc gggagccgta ataaacagca cgaacaagtt ctgcagccaa gcttctcgag gatccggctg ctaacaaagc ccgaaaggaa gctganttgg ctgctgccnc c

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gacagccaca accccgggcg aggccaccac accagaagcg cgcatcctcg ttgtcgacga cgaggacaac atcgtcgagc tgctctcggt cagtctgaag tttcagggat tcgaggtgca cacggcaacc aacggcgcgc aggcgttgga tcgggccagg gaaacccgcc cggacgcggt gattctcgat gtgatgatgc ccgggatgga cggcttcggc gtgctgcggc ggctgcgcgc cgacggcatc gatgcacccg cactgttctt gaccgcccgg gactcgctgc aggacaagat cgcggggctg accctgggcg gcgatgacta cgtgacaaag cccttcagcc tggaagaggt ggttgcccgg ttgcgggtaa ttctgcgccg cgccggcaag ggcagcgcag agccgcgcaa ttcccgcctt acgttcgccg atatcgagct cgatgaggaa acccacgaag tgtggaaggc cggccagccg gtgtcgctat cgcccaccga gttcaccttg ttgcgctatt tcgtgatcaa tgcgggcacg gtgctgagca agccgaaaat tctcgaccat gtgtggcgct acgacttcgg tggcgacgtc aacgtcgtcg agtcctacgt gtcgtatctg cggcgcaaga tcgataccgg cgacaagcgg ctgctgcaca ccttgcgtgg ggtcggctat gtgctgcggg agccgaaaca gcacgaacaa gttctgcagc caagcttctc gaggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaa