MymaA.00587.a

Transcriptional regulatory protein KdpE

CENTER ID: MymaA.00587.a
ORGANISM: Mycobacterium marinum ATCC BAA-535 / M
ASSOCIATED DISEASE: Cutaneous ulcerations, deep abscesses
CURRENT STATUS: soluble
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MymaA.00587.a.A1.GE30042 Full length( MymaA.00587.a ) 1 226
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|216594.6.peg.684
RefSeq: YP_001848951.1
UniProt: B2HPR9

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTRVLVIDDE PQILRALRIN FSVRGYDVVT AATGAAALRA AAEQRPDVVI LDLGLPDMSG IEVLAGLRGW FSAPVIVLSA RSDSSDKVQA LDAGADDYVT KPFGMDELLA RLRAAVRRAA TATDTDQPVI ETSSFTVDLA TKKVIKNGSE VHLTPTEWGM LEMLVRNRGK LVGREELLKE VWGPAYAKET HYLRVYLAQL RRKLEDDPAH PRHLLTEAGM GYRFDG
NT Sequence
ATGACGCGCG TTCTGGTTAT CGACGACGAG CCACAGATCC TGCGTGCCCT GCGCATCAAC TTCTCCGTGC GCGGCTACGA CGTGGTGACG GCCGCCACCG GAGCGGCGGC CCTGCGCGCG GCGGCCGAGC AACGACCCGA CGTGGTGATT CTGGACCTCG GCCTGCCCGA CATGTCCGGC ATCGAAGTGC TCGCCGGCCT GCGCGGCTGG TTCAGCGCAC CGGTGATCGT GCTGTCGGCG CGAAGCGATT CGTCGGACAA GGTGCAGGCC CTCGATGCCG GCGCCGACGA CTATGTGACC AAGCCGTTCG GCATGGACGA GCTGCTGGCC CGGCTCAGGG CCGCCGTGCG CCGGGCCGCG ACGGCCACCG ACACCGATCA GCCGGTCATC GAAACCAGCT CATTCACAGT GGATTTGGCG ACCAAGAAGG TGATCAAGAA CGGGTCGGAG GTTCATCTCA CACCCACCGA GTGGGGCATG CTGGAGATGC TGGTGCGCAA CCGCGGCAAG CTGGTCGGGC GCGAAGAGCT GCTCAAGGAG GTTTGGGGCC CGGCCTACGC CAAGGAAACC CACTACCTGC GTGTCTATCT CGCCCAGCTG CGCCGCAAGC TTGAGGACGA CCCCGCACAC CCACGCCACC TACTGACCGA AGCCGGCATG GGCTACCGCT TCGACGGC
Details for MymaA.00587.a.A1.GE30042
HARVESTED ON: 8/10/2010
SEQUENCED ON: 8/16/2010
EXPECTED MW: 27kDa
OBSERVED MW: 27kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMTRVLVIDD EPQILRALRI NFSVRGYDVV TAATGAAALR AAAEQRPDVV ILDLGLPDMS GIEVLAGLRG WFSAPVIVLS ARSDSSDKVQ ALDAGADDYV TKPFGMDELL ARLRAAVRRA ATATDTDQPV IETSSFTVDL ATKKVIKNGS EVHLTPTEWG MLEMLVRNRG KLVGREELLK EVWGPAYAKE THYLRVYLAQ LRRKLEDDPA HPRHLLTEAG MGYRFDK
Validated NT Sequence
ttatttgtcg aagcggtagc ccatgccggc ttcggtcagt aggtggcgtg ggtgtgcggg gtcgtcctca agcttgcggc gcagctgggc gagatagaca cgcaggtagt gggtttcctt ggcgtaggcc gggccccaaa cctccttgag cagctcttcg cgcccgacca gcttgccgcg gttgcgcacc agcatctcca gcatgcccca ctcggtgggt gtgagatgaa cctccgaccc gttcttgatc accttcttgg tcgccaaatc cactgtgaat gagctggttt cgatgaccgg ctgatcggtg tcggtggccg tcgcggcccg gcgcacggcg gccctgagcc gggccagcag ctcgtccatg ccgaacggct tggtcacata gtcgtcggcg ccggcatcga gggcctgcac cttgtccgac gaatcgcttc gcgccgacag cacgatcacc ggtgcgctga accagccgcg caggccggcg agcacttcga tgccggacat gtcgggcagg ccgaggtcca gaatcaccac gtcgggtcgt tgctcggccg ccgcgcgcag ggccgccgct ccggtggcgg ccgtcaccac gtcgtagccg cgcacggaga agttgatgcg cagggcacgc aggatctgtg gctcgtcgtc gataaccaga acgcgcgtca tcgaaccagg accctgggtc tgagcttcca gggtacccat atgatggtga tggtgatgag ccat
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMTRVLVIDD EPQILRALRI NFSVRGYDVV TAATGAAALR AAAEQRPDVV ILDLGLPDMS GIEVLAGLRG WFSAPVIVLS ARSDSSDKVQ ALDAGADDYV TKPFGMDELL ARLRAAVRRA ATATDTDQPV IETSSFTVDL ATKKVIKNGS EVHLTPTEWG MLEMLVRNRG KLVGREELLK EVWGPAYAKE THYLRVYLAQ LRRKLEDDPA HPRHLLTEAG MGYRFD
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gacgcgcgtt ctggttatcg acgacgagcc acagatcctg cgtgccctgc gcatcaactt ctccgtgcgc ggctacgacg tggtgacggc cgccaccgga gcggcggccc tgcgcgcggc ggccgagcaa cgacccgacg tggtgattct ggacctcggc ctgcccgaca tgtccggcat cgaagtgctc gccggcctgc gcggctggtt cagcgcaccg gtgatcgtgc tgtcggcgcg aagcgattcg tcggacaagg tgcaggccct cgatgccggc gccgacgact atgtgaccaa gccgttcggc atggacgagc tgctggcccg gctcagggcc gccgtgcgcc gggccgcgac ggccaccgac accgatcagc cggtcatcga aaccagctca ttcacagtgg atttggcgac caagaaggtg atcaagaacg ggtcggaggt tcatctcaca cccaccgagt ggggcatgct ggagatgctg gtgcgcaacc gcggcaagct ggtcgggcgc gaagagctgc tcaaggaggt ttggggcccg gcctacgcca aggaaaccca ctacctgcgt gtctatctcg cccagctgcg ccgcaagctt gaggacgacc ccgcacaccc acgccaccta ctgaccgaag ccggcatggg ctaccgcttc gacaaacagc acgaacaagt tctgcagcca agcttctcga ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaa