MymaA.00572.a

Unidentified antibiotic-transport ATP-binding protein ABC transporter

CENTER ID: MymaA.00572.a
ORGANISM: Mycobacterium marinum ATCC BAA-535 / M
ASSOCIATED DISEASE: Cutaneous ulcerations, deep abscesses
CURRENT STATUS: expressed
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MymaA.00572.a.A1.GE30041 Full length( MymaA.00572.a ) 1 315
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|216594.6.peg.4531
RefSeq: YP_001852483.1
UniProt: B2HS50

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MATDIGSTPI EIRGLTKNFG SVRALDELDL TVREGEVHGF LGPNGAGKST TIRILLGLVK ADSGSVRLLG GNPWTDAVEL HRQIAYVPGD VTLWPSLTGG EIIDLLARMR GGIDQDRRAE LIERFGLDPH KKSRTYSKGN RQKVSLISAF SSRARLLLLD EPSSGLDPLM ENVFQQCVDE ARGRGVTVLL SSHILAETEA LCERITIIRA GRTVESGSLA SMRHLSRTSI KAEMIGDPGD LTRIQGVEDV TVDGNTLHVQ VDSESLGELV RVLGEAGVRS LVSQPPTLEE LFLRHYSVGP DGEAQHRSGT GVAAR
NT Sequence
ATGGCCACTG ACATCGGCTC GACACCCATC GAGATCCGGG GTCTAACCAA GAACTTCGGG TCGGTGCGCG CACTCGATGA ACTGGACTTG ACGGTGCGCG AGGGAGAGGT TCACGGCTTC TTGGGCCCCA ACGGCGCCGG GAAGTCGACG ACCATCCGCA TCCTGCTGGG CCTGGTGAAG GCGGATAGCG GAAGTGTGCG CCTGTTGGGC GGCAATCCGT GGACCGACGC AGTCGAGCTG CATCGCCAGA TCGCCTACGT GCCCGGTGAT GTCACACTGT GGCCGTCGCT GACCGGCGGC GAGATCATTG ATCTGCTCGC CCGGATGCGC GGAGGTATCG ATCAAGATCG TCGTGCGGAG TTGATCGAGC GCTTCGGCCT GGACCCGCAC AAGAAGTCCC GAACGTACTC GAAGGGCAAC CGGCAGAAGG TTTCGCTGAT CTCCGCCTTT TCCTCACGGG CCCGGCTGCT GCTGTTGGAC GAACCCTCCA GCGGCTTGGA CCCATTGATG GAAAACGTGT TTCAGCAGTG CGTCGACGAA GCGCGCGGCA GGGGGGTGAC GGTGCTGCTG TCCAGCCACA TCCTGGCCGA AACCGAGGCG CTGTGTGAGC GGATAACCAT CATCCGGGCC GGAAGAACCG TGGAGAGCGG TTCGCTGGCC TCCATGCGCC ACCTCAGCCG CACCTCGATC AAGGCTGAAA TGATCGGCGA TCCAGGAGAT CTCACCAGGA TCCAAGGAGT CGAAGACGTC ACCGTCGACG GCAACACGCT ACATGTCCAG GTCGACAGCG AGAGCCTGGG CGAGCTGGTG CGGGTACTGG GCGAAGCCGG CGTACGCAGT CTCGTCAGCC AGCCACCGAC TCTGGAAGAG CTGTTTCTGC GTCACTACAG CGTTGGCCCC GATGGCGAGG CCCAACACCG CAGCGGCACC GGAGTCGCAG CGCGA
Details for MymaA.00572.a.A1.GE30041
HARVESTED ON: 8/10/2010
SEQUENCED ON: 8/16/2010
EXPECTED MW: 36kDa
OBSERVED MW: 36kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 95
PERCENT COVERAGE: 109
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMATDIGSTP IEIRGLTKNF GSVRALDELD LTVREGEVHG FLGPNGAGKS TTIRILLGLV KADSGSVRLL GGNPWTDAVE LHRQIAYVPG DVTLWPSLTG GEIIDLLARM RGGIDQDRRA ELIERFGLDP HKKSRTYSKG NRQKVSLISA FSSRARLLLL DEPSSGLDPL MENVFQQCVD EARGRGVTVL LSSHILAETE ALCERITIIR AGRTVESGSL ASMRHLSRTS IKAEMIGDPG DLTRIQGVED VTVDGNTLHV QVDSEXLGEL VRVLGEAGVR XLVSQPPTLE ELXLRXYSVG PXGXAXXXXX XXRXXXXXXE XSXXXSFXXX XLLXXXXXXX XXXXXXX
Validated NT Sequence
ttttgtttan ctttaagaag gagatatacc atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatggcca ctgacatcgg ctcgacaccc atcgagatcc ggggtctaac caagaacttc gggtcggtgc gcgcactcga tgaactggac ttgacggtgc gcgagggaga ggttcacggc ttcttgggcc ccaacggcgc cgggaagtcg acgaccatcc gcatcctgct gggcctggtg aaggcggata gcggaagtgt gcgcctgttg ggcggcaatc cgtggaccga cgcagtcgag ctgcatcgcc agatcgccta cgtgcccggt gatgtcacac tgtggccgtc gctgaccggc ggcgagatca ttgatctgct cgcccggatg cgcggaggta tcgatcaaga tcgtcgtgcg gagttgatcg agcgcttcgg cctggacccg cacaagaagt cccgaacgta ctcgaagggc aaccggcaga aggtttcgct gatctccgcc ttttcctcac gggcccggct gctgctgttg gacgaaccct ccagcggctt ggacccattg atggaaaacg tgtttcagca gtgcgtcgac gaagcgcgcg gcaggggggt gacggtgctg ctgtccagcc acatcctggc cgaaaccgag gcgctgtgtg agcggataac catcatccgg gccggaagaa ccgtggagag cggttcgctg gcctccatgc gccacctcag ccgcacctcg atcaaggctg aaatgatcgg cgatccagga gatctcacca ggatccaagg agtcgaagac gtcaccgtcg acggnaacac gctacatgtc caggtcgaca gcgagagnct gggcgagctg gtgcgggtac tgggcgaagc cggcgtacgc nntctcgtca gccagccncc gactctggaa gagctgttnc tgcgnnncta cagcgttggc cccnatggcg angcccnnnn ccncancnnc ncgantcgcn gcgnnannaa nnnncncgaa nnnagtnngc annnnagctt ctnnannnnc cnnctgctan naaannncnn aannannntg antngnnnnc ngnnnncnnn tg
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMATDIGSTP IEIRGLTKNF GSVRALDELD LTVREGEVHG FLGPNGAGKS TTIRILLGLV KADSGSVRLL GGNPWTDAVE LHRQIAYVPG DVTLWPSLTG GEIIDLLARM RGGIDQDRRA ELIERFGLDP HKKSRTYSKG NRQKVSLISA FSSRARLLLL DEPSSGLDPL MENVFQQCVD EARGRGVTVL LSSHILAETE ALCERITIIR AGRTVESGSL ASMRHLSRTS IKAEMIGDPG DLTRIQGVED VTVDGNTLHV QVDSESLGEL VRVLGEAGVR SLVSQPPTLE ELFLRHYSVG PDGEAQHRSG TGVAA
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggccactgac atcggctcga cacccatcga gatccggggt ctaaccaaga acttcgggtc ggtgcgcgca ctcgatgaac tggacttgac ggtgcgcgag ggagaggttc acggcttctt gggccccaac ggcgccggga agtcgacgac catccgcatc ctgctgggcc tggtgaaggc ggatagcgga agtgtgcgcc tgttgggcgg caatccgtgg accgacgcag tcgagctgca tcgccagatc gcctacgtgc ccggtgatgt cacactgtgg ccgtcgctga ccggcggcga gatcattgat ctgctcgccc ggatgcgcgg aggtatcgat caagatcgtc gtgcggagtt gatcgagcgc ttcggcctgg acccgcacaa gaagtcccga acgtactcga agggcaaccg gcagaaggtt tcgctgatct ccgccttttc ctcacgggcc cggctgctgc tgttggacga accctccagc ggcttggacc cattgatgga aaacgtgttt cagcagtgcg tcgacgaagc gcgcggcagg ggggtgacgg tgctgctgtc cagccacatc ctggccgaaa ccgaggcgct gtgtgagcgg ataaccatca tccgggccgg aagaaccgtg gagagcggtt cgctggcctc catgcgccac ctcagccgca cctcgatcaa ggctgaaatg atcggcgatc caggagatct caccaggatc caaggagtcg aagacgtcac cgtcgacggc aacacgctac atgtccaggt cgacagcgag agcctgggcg agctggtgcg ggtactgggc gaagccggcg tacgcagtct cgtcagccag ccaccgactc tggaagagct gtttctgcgt cactacagcg ttggccccga tggcgaggcc caacaccgca gcggcaccgg agtcgcagcg aaacagcacg aacaagttct gcagccaagc ttctcgagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aa