MymaA.00560.a

Proline iminopeptidase Pip

CENTER ID: MymaA.00560.a
ORGANISM: Mycobacterium marinum ATCC BAA-535 / M
ASSOCIATED DISEASE: Cutaneous ulcerations, deep abscesses
CURRENT STATUS: purified
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MymaA.00560.a.A1.GE30040 Full length( MymaA.00560.a ) 1 288
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MymaA.00560.a.A1.PS00875 Full length( MymaA.00560.a ) 1 288

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|216594.6.peg.5142
RefSeq: YP_001853048.1
UniProt: B2HG81

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTKPEATIAV PGGKVWSKRV GGGSGLPLLV VHGGPGLPHY YLSALQRLAG EREVIFWDQL GCGNSERPSD VDLWTMGRSV AEMDAVVRAL GLEAFHIFGN SWGGMLAQQY VLDVPSGAVS LTISNSTASI PRFADNVIRL KAELDPGTQA TIDRHEAAGT THSAEYQSAI TTWNETHLCR TLPWPAYLTE AFQNLGPEIF ETMFGPSDFR IVGTIRDWDV VDRLAEIALP TLLLAGKYDE CSPEDMREMH QRIAGSRFEF FECSAHMPFI EEPDRFDRVM RDFLRSND
NT Sequence
GTGACGAAAC CGGAGGCGAC AATCGCGGTC CCGGGCGGCA AGGTGTGGAG CAAGCGCGTC GGCGGCGGCT CCGGCCTTCC CCTGCTCGTG GTGCATGGCG GCCCGGGCCT GCCGCACTAC TACCTGTCAG CGCTGCAACG ACTGGCCGGC GAACGGGAAG TCATCTTCTG GGATCAACTT GGCTGCGGAA ACTCCGAACG CCCGTCCGAC GTGGATCTGT GGACAATGGG GCGCTCGGTG GCCGAGATGG ACGCCGTGGT GCGCGCACTC GGGCTGGAAG CCTTTCACAT CTTCGGCAAT TCCTGGGGCG GAATGCTCGC CCAGCAGTAT GTCCTGGACG TGCCATCGGG AGCCGTCAGC CTGACCATCT CCAACAGCAC CGCGTCCATT CCCCGGTTCG CCGACAACGT GATTCGGCTC AAGGCGGAGC TGGATCCTGG CACCCAAGCC ACCATTGACC GCCACGAAGC CGCCGGCACC ACACATTCGG CCGAATATCA GTCCGCAATC ACGACCTGGA ACGAAACGCA TCTGTGCCGC ACTCTCCCCT GGCCTGCCTA CCTCACTGAA GCATTCCAGA ATCTGGGTCC CGAGATCTTC GAAACGATGT TCGGGCCCAG CGACTTTCGG ATCGTCGGGA CCATTCGCGA TTGGGATGTG GTCGATCGGT TGGCCGAAAT CGCCCTGCCG ACACTGCTGC TCGCGGGCAA GTACGACGAA TGCTCGCCGG AAGACATGCG CGAGATGCAT CAACGGATTG CCGGCTCACG ATTCGAGTTC TTCGAGTGCA GCGCGCACAT GCCCTTCATC GAAGAACCAG ACCGGTTTGA CCGGGTGATG CGCGACTTCC TGCGCAGCAA CGAC
Details for MymaA.00560.a.A1.GE30040
HARVESTED ON: 8/10/2010
SEQUENCED ON: 8/16/2010
EXPECTED MW: 34kDa
OBSERVED MW: 34kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMVTKPEATI AVPGGKVWSK RVGGGSGLPL LVVHGGPGLP HYYLSALQRL AGEREVIFWD QLGCGNSERP SDVDLWTMGR SVAEMDAVVR ALGLEAFHIF GNSWGGMLAQ QYVLDVPSGA VSLTISNSTA SIPRFADNVI RLKAELDPGT QATIDRHEAA GTTHSAEYQS AITTWNETHL CRTLPWPAYL TEAFQNLGPE IFETMFGPSD FRIVGTIRDW DVVDRLAEIA LPTLLLAGKY DECSPEDMRE MHQRIAGSRF EFFECSAHMP FIEEPDRFDR VMRDFLRSN
Validated NT Sequence
ttattagttg ctgcgcagga agtcgcgcat cacccggtca aaccggtctg gttcttcgat gaagggcatg tgcgcgctgc actcgaagaa ctcgaatcgt gagccggcaa tccgttgatg catctcgcgc atgtcttccg gcgagcattc gtcgtacttg cccgcgagca gcagtgtcgg cagggcgatt tcggccaacc gatcgaccac atcccaatcg cgaatggtcc cgacgatccg aaagtcgctg ggcccgaaca tcgtttcgaa gatctcggga cccagattct ggaatgcttc agtgaggtag gcaggccagg ggagagtgcg gcacagatgc gtttcgttcc aggtcgtgat tgcggactga tattcggccg aatgtgtggt gccggcggct tcgtggcggt caatggtggc ttgggtgcca ggatccagct ccgccttgag ccgaatcacg ttgtcggcga accggggaat ggacgcggtg ctgttggaga tggtcaggct gacggctccc gatggcacgt ccaggacata ctgctgggcg agcattccgc cccaggaatt gccgaagatg tgaaaggctt ccagcccgag tgcgcgcacc acggcgtcca tctcggccac cgagcgcccc attgtccaca gatccacgtc ggacgggcgt tcggagtttc cgcagccaag ttgatcccag aagatgactt cccgttcgcc ggccagtcgt tgcagcgctg acaggtagta gtgcggcagg cccgggccgc catgcaccac gagcagggga aggccggagc cgccgccgac gcgcttgctc cacaccttgc cgcccgggac cgcgattgtc gcctccggtt tcgtcaccat cgaaccagga ccctgggtct gagcttccag ggtacccata tgatggtgat ggtgatgagc cat
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVTKPEATI AVPGGKVWSK RVGGGSGLPL LVVHGGPGLP HYYLSALQRL AGEREVIFWD QLGCGNSERP SDVDLWTMGR SVAEMDAVVR ALGLEAFHIF GNSWGGMLAQ QYVLDVPSGA VSLTISNSTA SIPRFADNVI RLKAELDPGT QATIDRHEAA GTTHSAEYQS AITTWNETHL CRTLPWPAYL TEAFQNLGPE IFETMFGPSD FRIVGTIRDW DVVDRLAEIA LPTLLLAGKY DECSPEDMRE MHQRIAGSRF EFFECSAHMP FIEEPDRFDR VMRDFLRSN
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtgacgaaa ccggaggcga caatcgcggt cccgggcggc aaggtgtgga gcaagcgcgt cggcggcggc tccggccttc ccctgctcgt ggtgcatggc ggcccgggcc tgccgcacta ctacctgtca gcgctgcaac gactggccgg cgaacgggaa gtcatcttct gggatcaact tggctgcgga aactccgaac gcccgtccga cgtggatctg tggacaatgg ggcgctcggt ggccgagatg gacgccgtgg tgcgcgcact cgggctggaa gcctttcaca tcttcggcaa ttcctggggc ggaatgctcg cccagcagta tgtcctggac gtgccatcgg gagccgtcag cctgaccatc tccaacagca ccgcgtccat tccccggttc gccgacaacg tgattcggct caaggcggag ctggatcctg gcacccaagc caccattgac cgccacgaag ccgccggcac cacacattcg gccgaatatc agtccgcaat cacgacctgg aacgaaacgc atctgtgccg cactctcccc tggcctgcct acctcactga agcattccag aatctgggtc ccgagatctt cgaaacgatg ttcgggccca gcgactttcg gatcgtcggg accattcgcg attgggatgt ggtcgatcgg ttggccgaaa tcgccctgcc gacactgctg ctcgcgggca agtacgacga atgctcgccg gaagacatgc gcgagatgca tcaacggatt gccggctcac gattcgagtt cttcgagtgc agcgcgcaca tgcccttcat cgaagaacca gaccggtttg accgggtgat gcgcgacttc ctgcgcagca acaaacagca cgaacaagtt ctgcagccaa gcttctcgag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaa
Details for MymaA.00560.a.A1.PS00875
PURIFICATION DATe: 11/23/2010
CONCENTRATION: 61.74mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Low Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 5
VIAL VOLUME: 100µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMVTKPEATI AVPGGKVWSK RVGGGSGLPL LVVHGGPGLP HYYLSALQRL AGEREVIFWD QLGCGNSERP SDVDLWTMGR SVAEMDAVVR ALGLEAFHIF GNSWGGMLAQ QYVLDVPSGA VSLTISNSTA SIPRFADNVI RLKAELDPGT QATIDRHEAA GTTHSAEYQS AITTWNETHL CRTLPWPAYL TEAFQNLGPE IFETMFGPSD FRIVGTIRDW DVVDRLAEIA LPTLLLAGKY DECSPEDMRE MHQRIAGSRF EFFECSAHMP FIEEPDRFDR VMRDFLRSN
Validated NT Sequence
ttattagttg ctgcgcagga agtcgcgcat cacccggtca aaccggtctg gttcttcgat gaagggcatg tgcgcgctgc actcgaagaa ctcgaatcgt gagccggcaa tccgttgatg catctcgcgc atgtcttccg gcgagcattc gtcgtacttg cccgcgagca gcagtgtcgg cagggcgatt tcggccaacc gatcgaccac atcccaatcg cgaatggtcc cgacgatccg aaagtcgctg ggcccgaaca tcgtttcgaa gatctcggga cccagattct ggaatgcttc agtgaggtag gcaggccagg ggagagtgcg gcacagatgc gtttcgttcc aggtcgtgat tgcggactga tattcggccg aatgtgtggt gccggcggct tcgtggcggt caatggtggc ttgggtgcca ggatccagct ccgccttgag ccgaatcacg ttgtcggcga accggggaat ggacgcggtg ctgttggaga tggtcaggct gacggctccc gatggcacgt ccaggacata ctgctgggcg agcattccgc cccaggaatt gccgaagatg tgaaaggctt ccagcccgag tgcgcgcacc acggcgtcca tctcggccac cgagcgcccc attgtccaca gatccacgtc ggacgggcgt tcggagtttc cgcagccaag ttgatcccag aagatgactt cccgttcgcc ggccagtcgt tgcagcgctg acaggtagta gtgcggcagg cccgggccgc catgcaccac gagcagggga aggccggagc cgccgccgac gcgcttgctc cacaccttgc cgcccgggac cgcgattgtc gcctccggtt tcgtcaccat cgaaccagga ccctgggtct gagcttccag ggtacccata tgatggtgat ggtgatgagc cat
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVTKPEATI AVPGGKVWSK RVGGGSGLPL LVVHGGPGLP HYYLSALQRL AGEREVIFWD QLGCGNSERP SDVDLWTMGR SVAEMDAVVR ALGLEAFHIF GNSWGGMLAQ QYVLDVPSGA VSLTISNSTA SIPRFADNVI RLKAELDPGT QATIDRHEAA GTTHSAEYQS AITTWNETHL CRTLPWPAYL TEAFQNLGPE IFETMFGPSD FRIVGTIRDW DVVDRLAEIA LPTLLLAGKY DECSPEDMRE MHQRIAGSRF EFFECSAHMP FIEEPDRFDR VMRDFLRSN
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGTGACGAAA CCGGAGGCGA CAATCGCGGT CCCGGGCGGC AAGGTGTGGA GCAAGCGCGT CGGCGGCGGC TCCGGCCTTC CCCTGCTCGT GGTGCATGGC GGCCCGGGCC TGCCGCACTA CTACCTGTCA GCGCTGCAAC GACTGGCCGG CGAACGGGAA GTCATCTTCT GGGATCAACT TGGCTGCGGA AACTCCGAAC GCCCGTCCGA CGTGGATCTG TGGACAATGG GGCGCTCGGT GGCCGAGATG GACGCCGTGG TGCGCGCACT CGGGCTGGAA GCCTTTCACA TCTTCGGCAA TTCCTGGGGC GGAATGCTCG CCCAGCAGTA TGTCCTGGAC GTGCCATCGG GAGCCGTCAG CCTGACCATC TCCAACAGCA CCGCGTCCAT TCCCCGGTTC GCCGACAACG TGATTCGGCT CAAGGCGGAG CTGGATCCTG GCACCCAAGC CACCATTGAC CGCCACGAAG CCGCCGGCAC CACACATTCG GCCGAATATC AGTCCGCAAT CACGACCTGG AACGAAACGC ATCTGTGCCG CACTCTCCCC TGGCCTGCCT ACCTCACTGA AGCATTCCAG AATCTGGGTC CCGAGATCTT CGAAACGATG TTCGGGCCCA GCGACTTTCG GATCGTCGGG ACCATTCGCG ATTGGGATGT GGTCGATCGG TTGGCCGAAA TCGCCCTGCC GACACTGCTG CTCGCGGGCA AGTACGACGA ATGCTCGCCG GAAGACATGC GCGAGATGCA TCAACGGATT GCCGGCTCAC GATTCGAGTT CTTCGAGTGC AGCGCGCACA TGCCCTTCAT CGAAGAACCA GACCGGTTTG ACCGGGTGAT GCGCGACTTC CTGCGCAGCA ACAAACAGCA CGAACAAGTT CTGCAGCCAA GCTTCTCGAG GATCCGGCTG CTAACAAAGC CCGAAAGGAA GCTGAGTTGG CTGCTGCCAC CGCTGAGCAA TAACTAGCAT AACCCCTTGG GGCCTCTAAA CGGGTCTTGA GGGGTTTTTT GCTGAAAGGA GGAACTATAT CCGGATATCC ACAGGACGGG TGTGGTCGCC ATGATCGCGT AGTCGATAGT GGCTCCAAGT AGCGAAGCGA GCAGGACTGG GCGGCGGCCA AAGCGGTCGG ACAGTGCTCC GAGAACGGGT GCGCATAGAA ATTGCATCAA CGCATATAGC GCTAGCAGCA CGCCATAGTG ACTGGCGATG CTGTCGGAAT GGACGATATC CCGCAAGAGG CCCGGCAGTA CCGGCATAAC CAAGCCTATG CCTACAGCAT CCAGGGTGAC GGTGCCGAGG ATGACGATGA GCGCATTGTT AGATTTCATA CACGGTGCCT GACTGCGTTA GCAATTTAAC TGTGATAAAC TACCGCATTA AAGCTTATCG ATGATAAGCT GTCAAACATG AGAA