SSGCID: Target Page

MymaA.00553.a

Recombination protein RecR (Rv3715c ortholog)

CENTER ID:	MymaA.00553.a
ORGANISM:	Mycobacterium marinum ATCC BAA-535 / M
ASSOCIATED DISEASE:	Cutaneous ulcerations, deep abscesses
CURRENT STATUS:	cloned
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MymaA.00553.a.B1.GE37191	full length		1	203

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|216594.6.peg.5596
RefSeq:	YP_001853491.1
UniProt:	B2HKD9

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MFEGPVQDLI DELGKLPGIG PKSAQRIAFH LLSVEPPDID RLTAVLAKVR DGVRFCAVCG NVSDDERCRI CADPRRDGSL VCVVEEPKDI QAVERTREYR GRYHVLGGAL DPLSGIGPEQ LRIRELLTRI GDRVDGVDIT EVIIATDPNT EGEATATYLV RMLRDIPGLT VTRIASGLPM GGDLEFADEL TLGRALTGRR AMV

NT Sequence

atgttcgagg gacccgttca ggatctgatc gacgagctcg gcaagctgcc gggcatcggc cccaagagtg cccagcgcat cgcgtttcac ctcttgtcgg ttgagccgcc ggacatcgac cggctgaccg ccgtgctggc caaggtccgg gacggggtgc gcttctgcgc tgtgtgcggg aacgtctccg atgacgagcg ttgccgcatt tgcgcggacc cgcgccggga cgggtccctg gtctgcgtcg tcgaagagcc caaagacatc caggccgtcg agcgaacccg cgaataccgc ggtcgctatc acgtgctggg cggggcgctc gatccgcttt ccgggatcgg tcccgagcag ctgcgaatcc gcgagttgct gactcgcatc ggtgatcggg tcgacggcgt cgatatcacc gaggtgatca tcgccaccga ccccaatacc gagggcgagg cgacggccac ctacctggtg cggatgctgc gcgacatccc cggcctgacc gtcacccgga tcgcgtcggg gctgccgatg ggtggtgacc tggagttcgc cgacgaactc accctgggcc gcgcgttgac cggccgccgc gcgatggtct ga

Details for MymaA.00553.a.B1.GE37191

HARVESTED ON:	data unavailable
SEQUENCED ON:	8/15/2013
EXPECTED MW:	23kDa
OBSERVED MW:	0kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Data Unavailable
SOLUBLE EXPRESSION LEVEL	Data Unavailable
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMF EGPVQDLIDE LGKLPGIGPK SAQRIAFHLL SVEPPDIDRL TAVLAKVRDG VRFCAVCGNV SDDERCRICA DPRRDGSLVC VVEEPKDIQA VERTREYRGR YHVLGGALDP LSGIGPEQLR IRELLTRIGD RVDGVDITEV IIATDPNTEG EATATYLVRM LRDIPGLTVT RIASGLPMGG DLEFADELTL GRALTGRRAM V

Validated NT Sequence

tttgtnnnnt ttaagaagga ganataccat ggctcaccac caccaccacc atatgttcga gggacccgtt caggatctga tcgacgagct cggcaagctg ccgggcatcg gccccaagag tgcccagcgc atcgcgtttc acctcttgtc ggttgagccg ccggacatcg accggctgac cgccgtgctg gccaaggtcc gggacggggt gcgcttctgc gctgtgtgcg ggaacgtctc cgatgacgag cgttgccgca tttgcgcgga cccgcgccgg gacgggtccc tggtctgcgt cgtcgaagag cccaaagaca tccaggccgt cgagcgaacc cgcgaatacc gcggtcgcta tcacgtgctg ggcggggcgc tcgatccgct ttccgggatc ggtcccgagc agctgcgaat ccgcgagttg ctgactcgca tcggtgatcg ggtcgacggc gtcgatatca ccgaggtgat catcgccacc gaccccaata ccgagggcga ggcgacggcc acctacctgg tgcggatgct gcgcgacatc cccggcctga ccgtcacccg gatcgcgtcg gggctgccga tgggtggtga cctggagttc gccgacgaac tcaccctggg ccgcgcgttg accggccgcc gcgcgatggt ctaagtgagt aagataggat ccggctgcta acannccccc nnnnnnnnnn nnnannnnnn ccnncnnncn nnnnncnnng tcncgcnnnc cntncccnnc nnnngcnnnc cncnncntcn cnnnnnacca gcnnncccct gcccacccnc ncgnncnnnc ccnnnnntgt nnnnnnnnnc cnnnnc

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgttcg agggacccgt tcaggatctg atcgacgagc tcggcaagct gccgggcatc ggccccaaga gtgcccagcg catcgcgttt cacctcttgt cggttgagcc gccggacatc gaccggctga ccgccgtgct ggccaaggtc cgggacgggg tgcgcttctg cgctgtgtgc gggaacgtct ccgatgacga gcgttgccgc atttgcgcgg acccgcgccg ggacgggtcc ctggtctgcg tcgtcgaaga gcccaaagac atccaggccg tcgagcgaac ccgcgaatac cgcggtcgct atcacgtgct gggcggggcg ctcgatccgc tttccgggat cggtcccgag cagctgcgaa tccgcgagtt gctgactcgc atcggtgatc gggtcgacgg cgtcgatatc accgaggtga tcatcgccac cgaccccaat accgagggcg aggcgacggc cacctacctg gtgcggatgc tgcgcgacat ccccggcctg accgtcaccc ggatcgcgtc ggggctgccg atgggtggtg acctggagtt cgccgacgaa ctcaccctgg gccgcgcgtt gaccggccgc cgcgcgatgg tctgagtaag ataggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaatt cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg cagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca tcagcgtggt cgtgaagcga ttcacagatg tctgcctgtt catccgcgtc cagctcgttg agtttctcca gaagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt tggtcactga tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa atcactcagg gtcaatgcca gcgcttcgtt aatacagatg taggtgttcc acagggtagc cagcagcatc ctgcgatgca gatccggaac ataatggtgc agggcgctga cttccgcgtt tccagacttt acgaaacacg gaaaccgaag accattcatg ttgttgctca ggtcgcagac gttttgcagc agcagtcgct tcacgttcgc tcgcgtatcg gtgattcatt ctgctaacca gtaaggcaac cccgccagcc tagccgggtc ctcaacgaca ggagcacgat catgcgcacc cgtggccagg acccaacgct gcccgagatg cgccgcgtgc ggctgctgga gatggcggac gcgatggata tgttctgcca agggttggtt tgcgcattca cagttctccg caagaattga ttggctccaa ttcttggagt ggtgaatccg ttagcgaggt gccgccggct tccattcagg tcgaggtggc ccggctccat gcaccgcgac gcaacgcggg gaggcagaca aggtataggg cggcgcctac aatccatgcc aacccgttcc atgtgctcgc cgaggcggca taaatcgccg tgacgatcag cggtccagtg atcgaagtta ggctggtaag agccgcgagc gatccttgaa gctgtccctg atggtcgtca tctacctgcc tggacagcat ggcctgcaac gcgggcatcc cgatgccgcc ggaagcgaga agaatcataa tggggaaggc catccagcct cgcgtcgcga acgccagcaa gacgtagccc agcgcgtcgg ccgccatgcc ggcgataatg gcctgcttct cgccgaaacg tttggtggcg ggaccagtga cgaaggcttg agcgagggcg tgcaagattc cgaataccgc aagcgacagg ccgatcatcg tcgcgctcca gcgaaagcgg tcctcgccga aaatgaccca gagcgctgcc ggcacctgtc ctacgagttg catgataaag aagacagtca taagtgcggc gacgatagtc atgccccgcg cccaccggaa ggagctgact gggttgaagg ctctcaaggg catcggtcga cgctctccct tatgcgactc ctgcattagg aagcagccca gtagtaggtt gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgc aaggagatgg cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa caagcgctca tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata taggcgccag caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag aggatcgaga tctcgatccc gcgaaat