MymaA.00471.a
2-hydroxyhepta-2,4-diene-1,7-dioate isomerase
| CENTER ID: | MymaA.00471.a |
| ORGANISM: | Mycobacterium marinum ATCC BAA-535 / M |
| ASSOCIATED DISEASE: | Cutaneous ulcerations, deep abscesses |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | False |
| NIH RISK GROUP: | 2 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
IIIC |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
Materials can be ordered from SSGCID using the button in the "order material" column.
Clicking the button will add the material to a virtual cart.
You may order multiple materials at a time at no cost to you, as this contract is funded
by NIAID. When you are ready to place your order, click the "Place Order" link which will
appear in the top right corner of the page after you place your first item in your cart.
Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| MymaA.00471.a.A1.GE30029 | Full length( MymaA.00471.a ) | 1 | 265 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| MymaA.00471.a.A1.PS00830 | Full length( MymaA.00471.a ) | 1 | 265 |
Structures
3QDF
DEPOSITED: 1/18/2011
DETERMINATION: XRay
CLONE: MymaA.00471.a.A1.GE30029
PROTEIN: MymaA.00471.a.A1.PS00830
Publications by SSGCID
Increasing the structural coverage of tuberculosis drug targets.
Abendroth J, Abramov A, Armour B, Barrett LK, Baugh L, Begley DW, Buchko GW, Choi R, Clifton MC, Dieterich SH, Dranow DM, Edwards TE, Fairman JW, Ferrell M, Fox D, Gardberg AS, Hewitt SN, Lorimer D, Lyons-Abbott S, Mundt E, Muruthi MM, Myers J, Myler PJ, Napuli AJ, Phan I, Sekar A, Serbzhinskiy D, Stacy R, Staker BL, Stewart LJ, Taylor BM, Thompkins K, Tran N, Van Voorhis WC, Zhang Y
Tuberculosis (Edinb) - 2014
volume 95, issue 2, pages 142-8
PMID: 25613812; PMCID: PMC4361283
PDB:
3GVC,
3GVG,
3GWC,
3H7F,
3H81,
3HE2,
3HWI,
3HWK,
3HZG,
3ICO,
3KHP,
3LLS,
3MOY,
3MPZ,
3MYB,
3NDN,
3NDO,
3NF4,
3NG3,
3NJD,
3NWO,
3O0M,
3O38,
3OC6,
3OC7,
3OEC,
3OI9,
3OKS,
3OL3,
3OL4,
3OME,
3P0T,
3P2Y,
3P4I,
3P4T,
3P5M,
3P85,
3PE8,
3PGX,
3PK0,
3PPI,
3PXX,
3PZY,
3Q1T,
3Q8N,
3QBP,
3QDF,
3QH4,
3QHA,
3QIV,
3QK8,
3QKA,
3QLJ,
3QMJ,
3QRE,
3QUA,
3QUV,
3QXI,
3QXZ,
3QYR,
3R0O,
3R1I,
3R1J,
3R20,
3R2N,
3R4T,
3R6H,
3R6O,
3R7K,
3R8C,
3R9P,
3R9Q,
3R9R,
3R9S,
3R9T,
3RD5,
3RD7,
3RD8,
3RFQ,
3RIH,
3RR2,
3RR6,
3RRP,
3RRV,
3RSI,
3RV2,
3S55,
3S82,
3SBX,
3SF6,
3SLL,
3SVK,
3SVT,
3SWO,
3SWT,
3SWX,
3SX2,
3T3W,
3T7C,
3TAV,
3TCR,
3TDE,
3TJR,
3TL3,
3TLF,
3TRR,
3TSC,
3TX2,
3TZQ,
3TZU,
3U0A,
3U0B,
3UCX,
3UVE,
4DI1,
4DIE,
4DQ8,
4DXL,
4ED4,
4EGE,
4EGF,
4EMD,
4EO9,
4EYE,
4F3W,
4F47,
4FFC,
4GK6,
4HDT,
4HR3,
4I1Y,
4IJN,
4IV6,
4IZ9,
4J5I,
4KAM,
4LGV,
4O2D,
5EJ2
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| OrthoMCL: | OG5_126763 |
| PATRIC ID: | fig|216594.6.peg.1838 |
| RefSeq: | YP_001850022.1 |
| UniProt: | B2HIH3 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MRLGRIASPD GVAFASIEGE LDNPGAMTAR EIAEHPFGTP NFTGRSWPLA DVRLLAPILA SKVVCVGKNY TDHIAEMGGQ TGPTPADPVI FLKPNTAIVG PNVPIRLPAN ASPVHFEGEL AIVIGRPCKD VSAAQAVDNI LGYTIGNDVS ARDQQKSDGQ WTRAKGHDTF CPVGPWIVTD VDPADLELRT EVNGAVKQHA RTSLMIHDVG AIVEWISAVM TLLPGDLILT GTPAGVGPIE DGDTVSITIE GIGTLTNPVV RKGKS
NT Sequence
ATGCGCCTTG GACGTATTGC CAGCCCTGAC GGGGTCGCCT TCGCCAGCAT CGAAGGCGAG TTAGACAATC CGGGTGCGAT GACCGCCCGG GAGATCGCGG AGCACCCTTT CGGGACGCCG AACTTCACCG GCCGCTCGTG GCCGTTGGCC GATGTGCGGC TGCTGGCCCC GATACTTGCC AGCAAGGTGG TCTGTGTCGG CAAGAACTAC ACCGATCACA TCGCGGAGAT GGGCGGCCAA ACCGGTCCGA CGCCGGCGGA TCCGGTGATA TTCCTCAAGC CCAACACGGC GATCGTCGGT CCGAACGTGC CGATTCGGTT GCCCGCCAAC GCATCACCAG TCCACTTCGA AGGCGAACTG GCGATTGTGA TCGGCCGGCC GTGTAAGGAC GTCTCGGCCG CGCAGGCGGT CGACAACATC CTCGGCTACA CCATCGGCAA TGACGTGTCC GCCCGCGATC AGCAGAAATC CGACGGGCAA TGGACCCGGG CCAAAGGCCA TGACACATTC TGTCCGGTCG GGCCATGGAT CGTTACCGAT GTCGACCCTG CCGACCTCGA ACTGCGCACC GAAGTCAATG GTGCGGTCAA GCAGCACGCG CGTACCTCGC TGATGATCCA CGATGTGGGT GCCATCGTGG AGTGGATCTC GGCGGTAATG ACATTGCTGC CCGGTGATCT CATCCTCACC GGAACACCCG CGGGGGTCGG TCCCATCGAA GACGGTGACA CCGTCTCCAT CACCATCGAG GGGATCGGCA CCCTGACCAA CCCCGTAGTC CGTAAAGGAA AGTCG
Details for MymaA.00471.a.A1.GE30029
| HARVESTED ON: | 8/10/2010 |
| SEQUENCED ON: | 8/16/2010 |
| EXPECTED MW: | 30kDa |
| OBSERVED MW: | 31kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Many (50-100) |
| TOTAL EXPRESSION LEVEL: | Low Expression |
| SOLUBLE EXPRESSION LEVEL | Moderate Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMRLGRIASP DGVAFASIEG ELDNPGAMTA REIAEHPFGT PNFTGRSWPL ADVRLLAPIL ASKVVCVGKN YTDHIAEMGG QTGPTPADPV IFLKPNTAIV GPNVPIRLPA NASPVHFEGE LAIVIGRPCK DVSAAQAVDN ILGYTIGNDV SARDQQKSDG QWTRAKGHDT FCPVGPWIVT DVDPADLELR TEVNGAVKQH ARTSLMIHDV GAIVEWISAV MTLLPGDLIL TGTPAGVGPI EDGDTVSITI EGIGTLTNPV VRKGK
Validated NT Sequence
ttattacttt cctttacgga ctacggggtt ggtcagggtg ccgatcccct cgatggtgat ggagacggtg tcaccgtctt cgatgggacc gacccccgcg ggtgttccgg tgaggatgag atcaccgggc agcaatgtca ttaccgccga gatccactcc acgatggcac ccacatcgtg gatcatcagc gaggtacgcg cgtgctgctt gaccgcacca ttgacttcgg tgcgcagttc gaggtcggca gggtcgacat cggtaacgat ccatggcccg accggacaga atgtgtcatg gcctttggcc cgggtccatt gcccgtcgga tttctgctga tcgcgggcgg acacgtcatt gccgatggtg tagccgagga tgttgtcgac cgcctgcgcg gccgagacgt ccttacacgg ccggccgatc acaatcgcca gttcgccttc gaagtggact ggtgatgcgt tggcgggcaa ccgaatcggc acgttcggac cgacgatcgc cgtgttgggc ttgaggaata tcaccggatc cgccggcgtc ggaccggttt ggccgcccat ctccgcgatg tgatcggtgt agttcttgcc gacacagacc accttgctgg caagtatcgg ggccagcagc cgcacatcgg ccaacggcca cgagcggccg gtgaagttcg gcgtcccgaa agggtgctcc gcgatctccc gggcggtcat cgcacccgga ttgtctaact cgccttcgat gctggcgaag gcgaccccgt cagggctggc aatacgtcca aggcgcatcg aaccaggacc ctgggtctga gcttccaggg tacccatatg atggtgatgg tgatgagcca t
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMRLGRIASP DGVAFASIEG ELDNPGAMTA REIAEHPFGT PNFTGRSWPL ADVRLLAPIL ASKVVCVGKN YTDHIAEMGG QTGPTPADPV IFLKPNTAIV GPNVPIRLPA NASPVHFEGE LAIVIGRPCK DVSAAQAVDN ILGYTIGNDV SARDQQKSDG QWTRAKGHDT FCPVGPWIVT DVDPADLELR TEVNGAVKQH ARTSLMIHDV GAIVEWISAV MTLLPGDLIL TGTPAGVGPI EDGDTVSITI EGIGTLTNPV VRKGK
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gcgccttgga cgtattgcca gccctgacgg ggtcgccttc gccagcatcg aaggcgagtt agacaatccg ggtgcgatga ccgcccggga gatcgcggag caccctttcg ggacgccgaa cttcaccggc cgctcgtggc cgttggccga tgtgcggctg ctggccccga tacttgccag caaggtggtc tgtgtcggca agaactacac cgatcacatc gcggagatgg gcggccaaac cggtccgacg ccggcggatc cggtgatatt cctcaagccc aacacggcga tcgtcggtcc gaacgtgccg attcggttgc ccgccaacgc atcaccagtc cacttcgaag gcgaactggc gattgtgatc ggccggccgt gtaaggacgt ctcggccgcg caggcggtcg acaacatcct cggctacacc atcggcaatg acgtgtccgc ccgcgatcag cagaaatccg acgggcaatg gacccgggcc aaaggccatg acacattctg tccggtcggg ccatggatcg ttaccgatgt cgaccctgcc gacctcgaac tgcgcaccga agtcaatggt gcggtcaagc agcacgcgcg tacctcgctg atgatccacg atgtgggtgc catcgtggag tggatctcgg cggtaatgac attgctgccc ggtgatctca tcctcaccgg aacacccgcg ggggtcggtc ccatcgaaga cggtgacacc gtctccatca ccatcgaggg gatcggcacc ctgaccaacc ccgtagtccg taaaggaaag aaacagcacg aacaagttct gcagccaagc ttctcgagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aa
Details for MymaA.00471.a.A1.PS00830
| PURIFICATION DATe: | 10/25/2010 |
| CONCENTRATION: | 26.1mg/ml |
| OBSERVED MW: | data unavailable |
| EXPRESSION LEVEL: | Low Expression |
| PROTEIN PURIFICATION BUFFER: | 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 3 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMRLGRIASP DGVAFASIEG ELDNPGAMTA REIAEHPFGT PNFTGRSWPL ADVRLLAPIL ASKVVCVGKN YTDHIAEMGG QTGPTPADPV IFLKPNTAIV GPNVPIRLPA NASPVHFEGE LAIVIGRPCK DVSAAQAVDN ILGYTIGNDV SARDQQKSDG QWTRAKGHDT FCPVGPWIVT DVDPADLELR TEVNGAVKQH ARTSLMIHDV GAIVEWISAV MTLLPGDLIL TGTPAGVGPI EDGDTVSITI EGIGTLTNPV VRKGK
Validated NT Sequence
ttattacttt cctttacgga ctacggggtt ggtcagggtg ccgatcccct cgatggtgat ggagacggtg tcaccgtctt cgatgggacc gacccccgcg ggtgttccgg tgaggatgag atcaccgggc agcaatgtca ttaccgccga gatccactcc acgatggcac ccacatcgtg gatcatcagc gaggtacgcg cgtgctgctt gaccgcacca ttgacttcgg tgcgcagttc gaggtcggca gggtcgacat cggtaacgat ccatggcccg accggacaga atgtgtcatg gcctttggcc cgggtccatt gcccgtcgga tttctgctga tcgcgggcgg acacgtcatt gccgatggtg tagccgagga tgttgtcgac cgcctgcgcg gccgagacgt ccttacacgg ccggccgatc acaatcgcca gttcgccttc gaagtggact ggtgatgcgt tggcgggcaa ccgaatcggc acgttcggac cgacgatcgc cgtgttgggc ttgaggaata tcaccggatc cgccggcgtc ggaccggttt ggccgcccat ctccgcgatg tgatcggtgt agttcttgcc gacacagacc accttgctgg caagtatcgg ggccagcagc cgcacatcgg ccaacggcca cgagcggccg gtgaagttcg gcgtcccgaa agggtgctcc gcgatctccc gggcggtcat cgcacccgga ttgtctaact cgccttcgat gctggcgaag gcgaccccgt cagggctggc aatacgtcca aggcgcatcg aaccaggacc ctgggtctga gcttccaggg tacccatatg atggtgatgg tgatgagcca t
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMRLGRIASP DGVAFASIEG ELDNPGAMTA REIAEHPFGT PNFTGRSWPL ADVRLLAPIL ASKVVCVGKN YTDHIAEMGG QTGPTPADPV IFLKPNTAIV GPNVPIRLPA NASPVHFEGE LAIVIGRPCK DVSAAQAVDN ILGYTIGNDV SARDQQKSDG QWTRAKGHDT FCPVGPWIVT DVDPADLELR TEVNGAVKQH ARTSLMIHDV GAIVEWISAV MTLLPGDLIL TGTPAGVGPI EDGDTVSITI EGIGTLTNPV VRKGK
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GCGCCTTGGA CGTATTGCCA GCCCTGACGG GGTCGCCTTC GCCAGCATCG AAGGCGAGTT AGACAATCCG GGTGCGATGA CCGCCCGGGA GATCGCGGAG CACCCTTTCG GGACGCCGAA CTTCACCGGC CGCTCGTGGC CGTTGGCCGA TGTGCGGCTG CTGGCCCCGA TACTTGCCAG CAAGGTGGTC TGTGTCGGCA AGAACTACAC CGATCACATC GCGGAGATGG GCGGCCAAAC CGGTCCGACG CCGGCGGATC CGGTGATATT CCTCAAGCCC AACACGGCGA TCGTCGGTCC GAACGTGCCG ATTCGGTTGC CCGCCAACGC ATCACCAGTC CACTTCGAAG GCGAACTGGC GATTGTGATC GGCCGGCCGT GTAAGGACGT CTCGGCCGCG CAGGCGGTCG ACAACATCCT CGGCTACACC ATCGGCAATG ACGTGTCCGC CCGCGATCAG CAGAAATCCG ACGGGCAATG GACCCGGGCC AAAGGCCATG ACACATTCTG TCCGGTCGGG CCATGGATCG TTACCGATGT CGACCCTGCC GACCTCGAAC TGCGCACCGA AGTCAATGGT GCGGTCAAGC AGCACGCGCG TACCTCGCTG ATGATCCACG ATGTGGGTGC CATCGTGGAG TGGATCTCGG CGGTAATGAC ATTGCTGCCC GGTGATCTCA TCCTCACCGG AACACCCGCG GGGGTCGGTC CCATCGAAGA CGGTGACACC GTCTCCATCA CCATCGAGGG GATCGGCACC CTGACCAACC CCGTAGTCCG TAAAGGAAAG AAACAGCACG AACAAGTTCT GCAGCCAAGC TTCTCGAGGA TCCGGCTGCT AACAAAGCCC GAAAGGAAGC TGAGTTGGCT GCTGCCACCG CTGAGCAATA ACTAGCATAA CCCCTTGGGG CCTCTAAACG GGTCTTGAGG GGTTTTTTGC TGAAAGGAGG AACTATATCC GGATATCCAC AGGACGGGTG TGGTCGCCAT GATCGCGTAG TCGATAGTGG CTCCAAGTAG CGAAGCGAGC AGGACTGGGC GGCGGCCAAA GCGGTCGGAC AGTGCTCCGA GAACGGGTGC GCATAGAAAT TGCATCAACG CATATAGCGC TAGCAGCACG CCATAGTGAC TGGCGATGCT GTCGGAATGG ACGATATCCC GCAAGAGGCC CGGCAGTACC GGCATAACCA AGCCTATGCC TACAGCATCC AGGGTGACGG TGCCGAGGAT GACGATGAGC GCATTGTTAG ATTTCATACA CGGTGCCTGA CTGCGTTAGC AATTTAACTG TGATAAACTA CCGCATTAAA GCTTATCGAT GATAAGCTGT CAAACATGAG AA