SSGCID: Target Page

MymaA.00429.b

6-phosphofructokinase PfkA_1

CENTER ID:	MymaA.00429.b
ORGANISM:	Mycobacterium marinum ATCC BAA-535 / M
ASSOCIATED DISEASE:	Cutaneous ulcerations, deep abscesses
CURRENT STATUS:	diffraction
COMMUNITY REQUEST:	False
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIC

Ordering Clones & Proteins

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Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MymaA.00429.b.A1.GE30025	Full length( MymaA.00429.b )		1	377

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MymaA.00429.b.A1.PS00878	Full length( MymaA.00429.b )		1	377

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|216594.6.peg.3652
RefSeq:	YP_001851691.1
UniProt:	B2HIS9

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MNSPIKRVAI STGGGDAPGL NAVIHAATLA ARNRGWDVVG IRDGLNGLLL PEDYPEGGLI ELTRDRVRGI IHQGGTIIGT TNRGNPTAYP VRQSDGSWAE VDRTEELLGR FAQHQIDALI LVGGDGSMSI GQHLHQSGLP LVGVPKTIDN DLDKTTSTFG FDSAVDFASE CIDRLFTTAT SHGRIIVVEV MGRYAGWIAL NAGMASGVHA ILIPEIPFSL EPVAAVITDR EQRGAKFSIV CVAEGAKPIG GEVTILGKSV GQAERLGGIG AKVAAELERM TGREARTVVL GHLLRGGSPT SFDRLLGLRF GAAAVRALDE GHYGVMVALN PPTVDYVPLA DATHRQKVVP LDCDSILTAR DMGINFGDEM PTHSTID

NT Sequence

GTGAATTCCC CGATAAAGCG AGTCGCGATC AGCACCGGTG GCGGGGACGC GCCGGGACTC AATGCGGTGA TCCACGCGGC CACTCTTGCC GCGCGAAACC GCGGCTGGGA CGTGGTCGGG ATCCGAGACG GCCTCAATGG GCTGTTGCTG CCCGAGGACT ATCCCGAGGG CGGCCTGATC GAGCTCACCC GCGACCGGGT TCGCGGGATC ATCCACCAGG GCGGCACGAT CATCGGCACG ACCAATCGGG GCAACCCGAC CGCCTACCCG GTGCGGCAGA GCGACGGTAG CTGGGCCGAA GTGGACCGCA CCGAAGAGCT ACTGGGCCGA TTCGCGCAGC ACCAGATCGA TGCGCTGATC CTGGTGGGCG GCGACGGGTC GATGTCGATC GGCCAGCATC TGCACCAGTC GGGGTTACCG CTGGTCGGCG TACCCAAGAC CATCGACAAT GACCTGGACA AGACGACGTC CACATTCGGC TTCGACAGCG CGGTCGACTT CGCCTCCGAG TGCATCGACC GGCTCTTCAC CACCGCGACC TCCCACGGCC GGATCATCGT GGTGGAGGTG ATGGGCCGGT ACGCGGGATG GATCGCCCTG AACGCCGGCA TGGCCTCGGG GGTGCACGCG ATCCTCATCC CCGAGATCCC GTTCAGTCTG GAGCCGGTGG CAGCTGTGAT CACCGATCGC GAACAGCGCG GCGCGAAATT CTCGATAGTC TGCGTCGCCG AGGGGGCCAA GCCGATCGGC GGCGAGGTCA CCATCCTGGG CAAGTCGGTG GGGCAGGCCG AACGCCTCGG TGGCATCGGC GCCAAGGTGG CCGCCGAGCT GGAACGCATG ACCGGCCGCG AAGCACGCAC GGTGGTATTG GGTCACCTGC TGCGGGGCGG CTCGCCCACC TCGTTCGACC GGCTACTGGG ACTGCGCTTC GGCGCCGCCG CCGTGCGGGC TCTCGATGAG GGGCACTACG GCGTCATGGT GGCGCTGAAC CCGCCGACGG TGGACTACGT ACCGCTTGCC GACGCCACCC ATCGGCAGAA GGTCGTTCCG CTCGATTGCG ACTCGATCCT GACCGCGCGC GACATGGGGA TCAACTTCGG CGACGAGATG CCCACCCACA GCACCATCGA T

Details for MymaA.00429.b.A1.GE30025

HARVESTED ON:	8/10/2010
SEQUENCED ON:	8/16/2010
EXPECTED MW:	42kDa
OBSERVED MW:	40kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Many (50-100)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	100
PERCENT COVERAGE:	79

Validated AA Sequence

MVNSPIKRVA ISTGGGDAPG LNAVIHAATL AARNRGWDVV GIRDGLNGLL LPEDYPEGGL IELTRDRVRG IIHQGGTIIG TTNRGNPTAY PVRQSDGSWA EVDRTEELLG RFAQHQIDAL ILVGGDGSMS IGQHLHQSGL PLVGVPKTID NDLDKTTSTF GFDSAVDFAS ECIDRLFTTA TSHGRIIVVE VMGRYAGWIA LNAGMASGVH AILIPEIPFS LEPVAAVITD REQRGAKFSI VCVAEGAKPI GGEVTILGKS VGQAERLGGI GAKVAAELER MTGREARTVV LGHLLRGGSP TSFDRLLGLR FGAAAVR

Validated NT Sequence

agcccgcacg gcggcggcgc cgaagcgcag tcccagtagc cggtcgaacg aggtgggcga gccgccccgc agcaggtgac ccaataccac cgtgcgtgct tcgcggccgg tcatgcgttc cagctcggcg gccaccttgg cgccgatgcc accgaggcgt tcggcctgcc ccaccgactt gcccaggatg gtgacctcgc cgccgatcgg cttggccccc tcggcgacgc agactatcga gaatttcgcg ccgcgctgtt cgcgatcggt gatcacagct gccaccggct ccagactgaa cgggatctcg gggatgagga tcgcgtgcac ccccgaggcc atgccggcgt tcagggcgat ccatcccgcg taccggccca tcacctccac cacgatgatc cggccgtggg aggtcgcggt ggtgaagagc cggtcgatgc actcggaggc gaagtcgacc gcgctgtcga agccgaatgt ggacgtcgtc ttgtccaggt cattgtcgat ggtcttgggt acgccgacca gcggtaaccc cgactggtgc agatgctggc cgatcgacat cgacccgtcg ccgcccacca ggatcagcgc atcgatctgg tgctgcgcga atcggcccag tagctcttcg gtgcggtcca cttcggccca gctaccgtcg ctctgccgca ccgggtaggc ggtcgggttg ccccgattgg tcgtgccgat gatcgtgccg ccctggtgga tgatcccgcg aacccggtcg cgggtgagct cgatcaggcc gccctcggga tagtcctcgg gcagcaacag cccattgagg ccgtctcgga tcccgaccac gtcccagccg cggtttcgcg cggcaagagt ggccgcgtgg atcaccgcat tgagtcccgg cgcgtccccg ccaccggtgc tgatcgcgac tcgctttatc ggggaattca ccatcgaacc aggaccctgg gtctgagctt ccagg

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMVNSPIKRV AISTGGGDAP GLNAVIHAAT LAARNRGWDV VGIRDGLNGL LLPEDYPEGG LIELTRDRVR GIIHQGGTII GTTNRGNPTA YPVRQSDGSW AEVDRTEELL GRFAQHQIDA LILVGGDGSM SIGQHLHQSG LPLVGVPKTI DNDLDKTTST FGFDSAVDFA SECIDRLFTT ATSHGRIIVV EVMGRYAGWI ALNAGMASGV HAILIPEIPF SLEPVAAVIT DREQRGAKFS IVCVAEGAKP IGGEVTILGK SVGQAERLGG IGAKVAAELE RMTGREARTV VLGHLLRGGS PTSFDRLLGL RFGAAAVRAL DEGHYGVMVA LNPPTVDYVP LADATHRQKV VPLDCDSILT ARDMGINFGD EMPTHSTI

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtgaattcc ccgataaagc gagtcgcgat cagcaccggt ggcggggacg cgccgggact caatgcggtg atccacgcgg ccactcttgc cgcgcgaaac cgcggctggg acgtggtcgg gatccgagac ggcctcaatg ggctgttgct gcccgaggac tatcccgagg gcggcctgat cgagctcacc cgcgaccggg ttcgcgggat catccaccag ggcggcacga tcatcggcac gaccaatcgg ggcaacccga ccgcctaccc ggtgcggcag agcgacggta gctgggccga agtggaccgc accgaagagc tactgggccg attcgcgcag caccagatcg atgcgctgat cctggtgggc ggcgacgggt cgatgtcgat cggccagcat ctgcaccagt cggggttacc gctggtcggc gtacccaaga ccatcgacaa tgacctggac aagacgacgt ccacattcgg cttcgacagc gcggtcgact tcgcctccga gtgcatcgac cggctcttca ccaccgcgac ctcccacggc cggatcatcg tggtggaggt gatgggccgg tacgcgggat ggatcgccct gaacgccggc atggcctcgg gggtgcacgc gatcctcatc cccgagatcc cgttcagtct ggagccggtg gcagctgtga tcaccgatcg cgaacagcgc ggcgcgaaat tctcgatagt ctgcgtcgcc gagggggcca agccgatcgg cggcgaggtc accatcctgg gcaagtcggt ggggcaggcc gaacgcctcg gtggcatcgg cgccaaggtg gccgccgagc tggaacgcat gaccggccgc gaagcacgca cggtggtatt gggtcacctg ctgcggggcg gctcgcccac ctcgttcgac cggctactgg gactgcgctt cggcgccgcc gccgtgcggg ctctcgatga ggggcactac ggcgtcatgg tggcgctgaa cccgccgacg gtggactacg taccgcttgc cgacgccacc catcggcaga aggtcgttcc gctcgattgc gactcgatcc tgaccgcgcg cgacatgggg atcaacttcg gcgacgagat gcccacccac agcaccatca aacagcacga acaagttctg cagccaagct tctcgaggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga a

Details for MymaA.00429.b.A1.PS00878

PURIFICATION DATe:	11/18/2010
CONCENTRATION:	23.11mg/ml
OBSERVED MW:	data unavailable
EXPRESSION LEVEL:	Moderate Expression
PROTEIN PURIFICATION BUFFER:	20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	2
VIAL VOLUME:	200µl
PERCENT IDENTITY:	100
PERCENT COVERAGE:	79

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGTGAATTCC CCGATAAAGC GAGTCGCGAT CAGCACCGGT GGCGGGGACG CGCCGGGACT CAATGCGGTG ATCCACGCGG CCACTCTTGC CGCGCGAAAC CGCGGCTGGG ACGTGGTCGG GATCCGAGAC GGCCTCAATG GGCTGTTGCT GCCCGAGGAC TATCCCGAGG GCGGCCTGAT CGAGCTCACC CGCGACCGGG TTCGCGGGAT CATCCACCAG GGCGGCACGA TCATCGGCAC GACCAATCGG GGCAACCCGA CCGCCTACCC GGTGCGGCAG AGCGACGGTA GCTGGGCCGA AGTGGACCGC ACCGAAGAGC TACTGGGCCG ATTCGCGCAG CACCAGATCG ATGCGCTGAT CCTGGTGGGC GGCGACGGGT CGATGTCGAT CGGCCAGCAT CTGCACCAGT CGGGGTTACC GCTGGTCGGC GTACCCAAGA CCATCGACAA TGACCTGGAC AAGACGACGT CCACATTCGG CTTCGACAGC GCGGTCGACT TCGCCTCCGA GTGCATCGAC CGGCTCTTCA CCACCGCGAC CTCCCACGGC CGGATCATCG TGGTGGAGGT GATGGGCCGG TACGCGGGAT GGATCGCCCT GAACGCCGGC ATGGCCTCGG GGGTGCACGC GATCCTCATC CCCGAGATCC CGTTCAGTCT GGAGCCGGTG GCAGCTGTGA TCACCGATCG CGAACAGCGC GGCGCGAAAT TCTCGATAGT CTGCGTCGCC GAGGGGGCCA AGCCGATCGG CGGCGAGGTC ACCATCCTGG GCAAGTCGGT GGGGCAGGCC GAACGCCTCG GTGGCATCGG CGCCAAGGTG GCCGCCGAGC TGGAACGCAT GACCGGCCGC GAAGCACGCA CGGTGGTATT GGGTCACCTG CTGCGGGGCG GCTCGCCCAC CTCGTTCGAC CGGCTACTGG GACTGCGCTT CGGCGCCGCC GCCGTGCGGG CTCTCGATGA GGGGCACTAC GGCGTCATGG TGGCGCTGAA CCCGCCGACG GTGGACTACG TACCGCTTGC CGACGCCACC CATCGGCAGA AGGTCGTTCC GCTCGATTGC GACTCGATCC TGACCGCGCG CGACATGGGG ATCAACTTCG GCGACGAGAT GCCCACCCAC AGCACCATCA AACAGCACGA ACAAGTTCTG CAGCCAAGCT TCTCGAGGAT CCGGCTGCTA ACAAAGCCCG AAAGGAAGCT GAGTTGGCTG CTGCCACCGC TGAGCAATAA CTAGCATAAC CCCTTGGGGC CTCTAAACGG GTCTTGAGGG GTTTTTTGCT GAAAGGAGGA ACTATATCCG GATATCCACA GGACGGGTGT GGTCGCCATG ATCGCGTAGT CGATAGTGGC TCCAAGTAGC GAAGCGAGCA GGACTGGGCG GCGGCCAAAG CGGTCGGACA GTGCTCCGAG AACGGGTGCG CATAGAAATT GCATCAACGC ATATAGCGCT AGCAGCACGC CATAGTGACT GGCGATGCTG TCGGAATGGA CGATATCCCG CAAGAGGCCC GGCAGTACCG GCATAACCAA GCCTATGCCT ACAGCATCCA GGGTGACGGT GCCGAGGATG ACGATGAGCG CATTGTTAGA TTTCATACAC GGTGCCTGAC TGCGTTAGCA ATTTAACTGT GATAAACTAC CGCATTAAAG CTTATCGATG ATAAGCTGTC AAACATGAGA A