MymaA.00429.a

6-phosphofructokinase PfkA

CENTER ID: MymaA.00429.a
ORGANISM: Mycobacterium marinum ATCC BAA-535 / M
ASSOCIATED DISEASE: Cutaneous ulcerations, deep abscesses
CURRENT STATUS: soluble
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MymaA.00429.a.A1.GE30024 Full length( MymaA.00429.a ) 1 343
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|216594.6.peg.1822
RefSeq: YP_001850007.1
UniProt: B2HIF8

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MRIGVLTGGG DCPGLNAVIR AVVRTCDARY GSSVVGFQDG WRGLLENRRL QLRNDDRNDR LLAKGGTMLG TARVHPDKLR AGLPQIKQTL DDNGIDVLIP IGGEGTLTAA HWLSEENVPV VGVPKTIDND IDCTDVTFGH DTALMVATEA IDRLHSTAES HQRVMLVEVM GRHAGWIALN SGLASGAHMT LIPEQPFDIE EVCRLVKQRF QRGDSHFICV VAEGAKPVSG SMQLREGGLD EFGHERFTGV AAQLGWEVEK RINKDVRVTV LGHVQRGGQP TAYDRVLATR FGVNAADAAH AGEYGQMVSL RGQDIGRVPL ADAVRQLKLV PQSRYDDAAE FFG
NT Sequence
ATGCGGATTG GAGTTCTCAC GGGAGGCGGC GACTGTCCAG GGCTGAATGC GGTGATCCGG GCGGTGGTGC GTACTTGCGA CGCCCGGTAC GGCTCATCGG TGGTCGGATT TCAGGACGGC TGGCGCGGGT TGCTGGAGAA CCGGCGGCTG CAGTTGCGCA ACGACGACCG CAACGACCGG CTGCTGGCCA AGGGCGGAAC GATGCTCGGC ACCGCCCGCG TACACCCCGA CAAACTGCGG GCCGGGTTGC CCCAGATCAA ACAGACGTTA GACGACAACG GCATTGACGT GCTCATCCCG ATCGGCGGCG AAGGCACGCT GACCGCCGCG CACTGGCTGT CCGAAGAGAA CGTTCCGGTG GTCGGCGTGC CCAAGACGAT CGACAACGAC ATCGACTGCA CCGATGTGAC TTTCGGCCAC GACACGGCCT TGATGGTGGC AACCGAGGCC ATCGACAGGC TGCACAGCAC CGCGGAATCC CACCAGCGGG TGATGCTGGT CGAGGTGATG GGTAGGCATG CGGGCTGGAT CGCGCTGAAC TCCGGGCTGG CATCTGGCGC ACACATGACG CTCATCCCTG AGCAGCCCTT CGACATCGAA GAGGTGTGCC GTCTGGTCAA ACAGCGCTTC CAGCGAGGCG ATTCGCACTT CATCTGTGTG GTCGCCGAGG GCGCCAAGCC CGTTTCGGGC TCGATGCAGT TGCGCGAGGG CGGACTCGAC GAATTCGGTC ACGAACGATT CACCGGCGTC GCCGCTCAGC TCGGCTGGGA AGTGGAAAAG CGCATCAACA AGGATGTCCG GGTGACCGTG CTGGGCCACG TGCAACGGGG CGGACAGCCG ACGGCCTACG ACCGGGTGCT GGCCACCCGG TTCGGCGTCA ATGCCGCTGA CGCCGCACAT GCGGGCGAAT ACGGCCAGAT GGTGTCGCTG CGCGGTCAGG ACATCGGCCG TGTACCCCTG GCCGACGCCG TTCGACAGCT CAAGCTGGTA CCGCAGAGCC GCTACGACGA CGCCGCTGAG TTCTTCGGC
Details for MymaA.00429.a.A1.GE30024
HARVESTED ON: 8/10/2010
SEQUENCED ON: 8/16/2010
EXPECTED MW: 39kDa
OBSERVED MW: 39kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 99
PERCENT COVERAGE: 81
Validated AA Sequence
MGTLEAQTQG PGSMRIGVLT GGGDCPGLNA VIRAVVRTCD ARYGSSVVGF QDGWRGLLEN RRLQLRNDDR NDRLLAKGGT MLGTARVHPD KLRAGLPQIK QTLDDNGIDV LIPIGGEGTL TAAHWLSEEN VPVVGVPKTI DNDIDCTDVT FGHDTALMVA TEAIDRLHST AESHQRVMLV EVMGRHAGWI ALNSGLASGA HMTLIPEQPF DIEEVCRLVK QRFQRGDSHF ICVVAEGAKP VSGSMQLREG GLDEFGHERF TGVAAQLGWE VEKRINKDVR VTVLGHVQRG GQPTAYX
Validated NT Sequence
cnggtngtag gccgtcggct gtccgccccg ttgcacgtgg cccagcacgg tcacccggac atccttgttg atgcgctttt ccacttccca gccgagctga gcggcgacgc cggtgaatcg ttcgtgaccg aattcgtcga gtccgccctc gcgcaactgc atcgagcccg aaacgggctt ggcgccctcg gcgaccacac agatgaagtg cgaatcgcct cgctggaagc gctgtttgac cagacggcac acctcttcga tgtcgaaggg ctgctcaggg atgagcgtca tgtgtgcgcc agatgccagc ccggagttca gcgcgatcca gcccgcatgc ctacccatca cctcgaccag catcacccgc tggtgggatt ccgcggtgct gtgcagcctg tcgatggcct cggttgccac catcaaggcc gtgtcgtggc cgaaagtcac atcggtgcag tcgatgtcgt tgtcgatcgt cttgggcacg ccgaccaccg gaacgttctc ttcggacagc cagtgcgcgg cggtcagcgt gccttcgccg ccgatcggga tgagcacgtc aatgccgttg tcgtctaacg tctgtttgat ctggggcaac ccggcccgca gtttgtcggg gtgtacgcgg gcggtgccga gcatcgttcc gcccttggcc agcagccggt cgttgcggtc gtcgttgcgc aactgcagcc gccggttctc cagcaacccg cgccagccgt cctgaaatcc gaccaccgat gagccgtacc gggcgtcgca agtacgcacc accgcccgga tcaccgcatt cagccctgga cagtcgccgc ctcccgtgag aactccaatc cgcatcgaac caggaccctg ggtctgagct tccagggtac ccatatgatg g
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMRIGVLTGG GDCPGLNAVI RAVVRTCDAR YGSSVVGFQD GWRGLLENRR LQLRNDDRND RLLAKGGTML GTARVHPDKL RAGLPQIKQT LDDNGIDVLI PIGGEGTLTA AHWLSEENVP VVGVPKTIDN DIDCTDVTFG HDTALMVATE AIDRLHSTAE SHQRVMLVEV MGRHAGWIAL NSGLASGAHM TLIPEQPFDI EEVCRLVKQR FQRGDSHFIC VVAEGAKPVS GSMQLREGGL DEFGHERFTG VAAQLGWEVE KRINKDVRVT VLGHVQRGGQ PTAYDRVLAT RFGVNAADAA HAGEYGQMVS LRGQDIGRVP LADAVRQLKL VPQSRYDDAA EFF
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gcggattgga gttctcacgg gaggcggcga ctgtccaggg ctgaatgcgg tgatccgggc ggtggtgcgt acttgcgacg cccggtacgg ctcatcggtg gtcggatttc aggacggctg gcgcgggttg ctggagaacc ggcggctgca gttgcgcaac gacgaccgca acgaccggct gctggccaag ggcggaacga tgctcggcac cgcccgcgta caccccgaca aactgcgggc cgggttgccc cagatcaaac agacgttaga cgacaacggc attgacgtgc tcatcccgat cggcggcgaa ggcacgctga ccgccgcgca ctggctgtcc gaagagaacg ttccggtggt cggcgtgccc aagacgatcg acaacgacat cgactgcacc gatgtgactt tcggccacga cacggccttg atggtggcaa ccgaggccat cgacaggctg cacagcaccg cggaatccca ccagcgggtg atgctggtcg aggtgatggg taggcatgcg ggctggatcg cgctgaactc cgggctggca tctggcgcac acatgacgct catccctgag cagcccttcg acatcgaaga ggtgtgccgt ctggtcaaac agcgcttcca gcgaggcgat tcgcacttca tctgtgtggt cgccgagggc gccaagcccg tttcgggctc gatgcagttg cgcgagggcg gactcgacga attcggtcac gaacgattca ccggcgtcgc cgctcagctc ggctgggaag tggaaaagcg catcaacaag gatgtccggg tgaccgtgct gggccacgtg caacggggcg gacagccgac ggcctacgac cgggtgctgg ccacccggtt cggcgtcaat gccgctgacg ccgcacatgc gggcgaatac ggccagatgg tgtcgctgcg cggtcaggac atcggccgtg tacccctggc cgacgccgtt cgacagctca agctggtacc gcagagccgc tacgacgacg ccgctgagtt cttcaaacag cacgaacaag ttctgcagcc aagcttctcg aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaa