MymaA.00416.c

ATPase component of an ABC-type transport system

CENTER ID: MymaA.00416.c
ORGANISM: Mycobacterium marinum ATCC BAA-535 / M
ASSOCIATED DISEASE: Cutaneous ulcerations, deep abscesses
CURRENT STATUS: expressed
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

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Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MymaA.00416.c.A1.GE30023 Full length( MymaA.00416.c ) 1 272
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|216594.6.peg.5435
RefSeq: YP_001853331.1
UniProt: B2HJ13

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MNQSNHPAIA LRGARLAFGD RVLWEDLDLS VSRGEFIAVL GPNGTGKTSL LKVLLGELAL SAGVALVDGA RLDTGRTRRG HIGYVPQHHP IDREVMLRGR DLVGLGVDGC RWGAVALRSG ERARRREAVQ RALQQVDGER LADVRVGLMS GGELQRIRVA QALASDPSLL LCDEPLLALD PANANLVAAL LDRRRREAAT AVMVVTHEVN PILPYVDRVL YLVDGRFLIG TVEQVMTSQT LSTLYRADIE VIKVVKGPHT RYLVVDAQDG LR
NT Sequence
TTGAACCAGT CGAACCACCC GGCAATTGCG CTGCGCGGTG CGCGGCTGGC ATTCGGCGAT CGGGTCCTGT GGGAGGACCT CGATCTGTCG GTGTCACGCG GCGAATTCAT CGCCGTGCTC GGGCCCAACG GCACCGGCAA GACCTCGTTG CTCAAGGTGT TACTCGGCGA ACTCGCGTTG AGCGCAGGTG TGGCGCTGGT GGACGGCGCC AGGCTCGACA CCGGTCGAAC GCGCCGCGGC CATATCGGCT ATGTCCCACA GCACCATCCG ATCGACCGGG AGGTGATGCT GCGCGGGCGC GACCTGGTCG GGCTGGGTGT CGACGGATGC CGGTGGGGTG CGGTTGCATT GCGCTCGGGC GAGCGGGCCC GTCGCCGCGA AGCCGTGCAG CGGGCGCTGC AGCAGGTCGA CGGTGAGCGG CTTGCCGACG TCCGGGTGGG GCTGATGTCG GGTGGCGAGC TGCAGCGGAT ACGCGTCGCC CAGGCACTGG CAAGCGACCC GTCGTTGCTG CTGTGCGATG AACCGCTACT GGCCCTCGAC CCGGCCAACG CCAACCTGGT CGCCGCCCTA CTCGATCGCC GTCGCCGGGA GGCCGCAACC GCGGTCATGG TGGTCACCCA CGAGGTCAAC CCGATCTTGC CGTACGTGGA CCGGGTGCTC TACCTGGTCG ACGGGCGATT CCTGATCGGC ACCGTCGAGC AGGTGATGAC CAGCCAGACC CTCTCGACGC TGTACCGCGC CGATATCGAG GTGATCAAAG TCGTCAAGGG CCCCCACACC CGCTATCTGG TAGTCGACGC CCAGGACGGG CTCCGA
Details for MymaA.00416.c.A1.GE30023
HARVESTED ON: 8/10/2010
SEQUENCED ON: 8/16/2010
EXPECTED MW: 31kDa
OBSERVED MW: 32kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 99
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMLNQSNHPA IALRGARLAF GDRVLWEDLD LSVSRGEFIA VLGPNGTGKT SLLKVLLGEL ALSAGVALVD GARLDTGRTR RGHIGYVPQH HPIDREVMLR GRDLVGLGVD GCRWGAVALR SGERARRREA VQRALQQVDG ERLADVRVGL MSGGELQRIR VAQALASDPS LLLCDEPLLA LDPANANLVA ALLDRRRREA ATAVMVVTHE VNPILPYVDR VLYLVDGRFL IGTVEQVMTS QTLSTLYRAD IEVIKVVKGP HTRYLVVDAX DGL
Validated NT Sequence
ttttgtttnn ctttaagaag gagatatacc atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgttga accagtcgaa ccacccggca attgcgctgc gcggtgcgcg gctggcattc ggcgatcggg tcctgtggga ggacctcgat ctgtcggtgt cacgcggcga attcatcgcc gtgctcgggc ccaacggcac cggcaagacc tcgttgctca aggtgttact cggcgaactc gcgttgagcg caggtgtggc gctggtggac ggcgccaggc tcgacaccgg tcgaacgcgc cgcggccata tcggctatgt cccacagcac catccgatcg accgggaggt gatgctgcgc gggcgcgacc tggtcgggct gggtgtcgac ggatgccggt ggggtgcggt tgcattgcgc tcgggcgagc gggcccgtcg ccgcgaagcc gtgcagcggg cgctgcagca ggtcgacggt gagcggcttg ccgacgtccg ggtggggctg atgtcgggtg gcgagctgca gcggatacgc gtcgcccagg cactggcaag cgacccgtcg ttgctgctgt gcgatgaacc gctactggcc ctcgacccgg ccaacgccaa cctggtcgcc gccctactcg atcgccgtcg ccgggaggcc gcaaccgcgg tcatggtggt cacccacgag gtcaacccga tcttgccgta cgtggaccgg gtgctctacc tggtcgacgg gcgattcctg atcggcaccg tcgagcaggt gatgaccagc cagaccctct cgacgctgta ccgcgccgat atcgaggtga tcaaagtcgt caagggcccc cacacccgct atctggtagt cgacgcccan gacgggctct aataanagca cgaacaagtt ctgcagccnn gcttctcgag gntccggctg ctaacangcc cgaaaggaag ctgantnngc tgctgc
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMLNQSNHPA IALRGARLAF GDRVLWEDLD LSVSRGEFIA VLGPNGTGKT SLLKVLLGEL ALSAGVALVD GARLDTGRTR RGHIGYVPQH HPIDREVMLR GRDLVGLGVD GCRWGAVALR SGERARRREA VQRALQQVDG ERLADVRVGL MSGGELQRIR VAQALASDPS LLLCDEPLLA LDPANANLVA ALLDRRRREA ATAVMVVTHE VNPILPYVDR VLYLVDGRFL IGTVEQVMTS QTLSTLYRAD IEVIKVVKGP HTRYLVVDAQ DGL
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gttgaaccag tcgaaccacc cggcaattgc gctgcgcggt gcgcggctgg cattcggcga tcgggtcctg tgggaggacc tcgatctgtc ggtgtcacgc ggcgaattca tcgccgtgct cgggcccaac ggcaccggca agacctcgtt gctcaaggtg ttactcggcg aactcgcgtt gagcgcaggt gtggcgctgg tggacggcgc caggctcgac accggtcgaa cgcgccgcgg ccatatcggc tatgtcccac agcaccatcc gatcgaccgg gaggtgatgc tgcgcgggcg cgacctggtc gggctgggtg tcgacggatg ccggtggggt gcggttgcat tgcgctcggg cgagcgggcc cgtcgccgcg aagccgtgca gcgggcgctg cagcaggtcg acggtgagcg gcttgccgac gtccgggtgg ggctgatgtc gggtggcgag ctgcagcgga tacgcgtcgc ccaggcactg gcaagcgacc cgtcgttgct gctgtgcgat gaaccgctac tggccctcga cccggccaac gccaacctgg tcgccgccct actcgatcgc cgtcgccggg aggccgcaac cgcggtcatg gtggtcaccc acgaggtcaa cccgatcttg ccgtacgtgg accgggtgct ctacctggtc gacgggcgat tcctgatcgg caccgtcgag caggtgatga ccagccagac cctctcgacg ctgtaccgcg ccgatatcga ggtgatcaaa gtcgtcaagg gcccccacac ccgctatctg gtagtcgacg cccaggacgg gctcaaacag cacgaacaag ttctgcagcc aagcttctcg aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaa