MymaA.00414.a

Short-chain type dehydrogenase/reductase

CENTER ID: MymaA.00414.a
ORGANISM: Mycobacterium marinum ATCC BAA-535 / M
ASSOCIATED DISEASE: Cutaneous ulcerations, deep abscesses
CURRENT STATUS: diffraction
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MymaA.00414.a.A1.GE30019 Full length( MymaA.00414.a ) 1 277
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MymaA.00414.a.A1.PS00811 Full length( MymaA.00414.a ) 1 277

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|216594.6.peg.5322
RefSeq: YP_001853227.1
UniProt: B2HHX2

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MQLSFQDRTY LVTGGGSGIG KGVAAGLVAA GASVMIVGRN PDKLAGAVQE LEALGANGGA IRYEPTDITN EDETARAVDA VTAWHGRLHG VVHCAGGSET IGPITQVDSE AWRRTVDLNV NGTMYVLKHA AREMVRGGGG SFVGISSIAA SNTHRWFGAY GVTKSAVDHL MQLAADELGA SWVRVNSIRP GLIRTDLVAA ITESAELSSD YAMCTPLPRQ GEVEDVANMA MFLLSDAASF VTGQVINVDG GQMLRRGPDF SAMLEPVFGR DALRGLV
NT Sequence
ATGCAGCTTT CGTTCCAAGA CCGGACCTAC CTGGTCACCG GCGGCGGTAG CGGAATCGGA AAGGGAGTGG CCGCCGGGCT GGTCGCGGCC GGAGCCTCGG TCATGATCGT CGGCCGTAAC CCCGACAAGC TCGCCGGCGC GGTACAGGAA CTCGAAGCGT TAGGTGCCAA CGGCGGCGCC ATCCGGTACG AACCCACCGA CATCACCAAC GAGGACGAGA CCGCCCGCGC GGTGGATGCG GTTACCGCCT GGCACGGCCG GCTGCATGGA GTGGTGCACT GTGCGGGCGG ATCGGAGACC ATCGGACCGA TCACCCAGGT CGACTCCGAA GCGTGGCGCC GTACGGTCGA CCTCAACGTC AACGGCACCA TGTACGTCCT CAAACACGCC GCACGAGAGA TGGTGCGAGG CGGCGGCGGA TCGTTTGTCG GCATCTCCTC GATTGCGGCC AGCAATACCC ATCGCTGGTT CGGTGCCTAC GGTGTCACCA AGTCCGCGGT CGATCACCTG ATGCAGTTGG CCGCCGACGA GCTCGGCGCG TCGTGGGTGC GGGTAAACAG CATCCGCCCC GGCTTGATCC GCACCGACCT CGTCGCAGCG ATCACCGAGT CCGCGGAGTT GAGCTCCGAC TACGCGATGT GCACGCCGCT GCCCCGCCAG GGTGAGGTCG AGGACGTCGC CAACATGGCG ATGTTTCTGC TCAGCGATGC CGCCAGCTTC GTCACCGGAC AGGTCATCAA TGTCGACGGC GGCCAGATGC TGCGGCGCGG CCCGGACTTC TCGGCGATGC TGGAACCGGT GTTCGGCCGT GACGCACTGC GCGGATTGGT C
Details for MymaA.00414.a.A1.GE30019
HARVESTED ON: 8/10/2010
SEQUENCED ON: 8/16/2010
EXPECTED MW: 31kDa
OBSERVED MW: 32kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 92
Validated AA Sequence
MQLSFQDRTY LVTGGGSGIG KGVAAGLVAA GASVMIVGRN PDKLAGAVQE LEALGANGGA IRYEPTDITN EDETARAVDA VTAWHGRLHG VVHCAGGSET IGPITQVDSE AWRRTVDLNV NGTMYVLKHA AREMVRGGGG SFVGISSIAA SNTHRWFGAY GVTKSAVDHL MQLAADELGA SWVRVNSIRP GLIRTDLVAA ITESAELSSD YAMCTPLPRQ GEVEDVANMA MFLLSDAASF VTGQVINVDG GQMLRRGPDF SAMLEPVFGR DALRGL
Validated NT Sequence
ttattacaat ccgcgcagtg cgtcacggcc gaacaccggt tccagcatcg ccgagaagtc cgggccgcgc cgcagcatct ggccgccgtc gacattgatg acctgtccgg tgacgaagct ggcggcatcg ctgagcagaa acatcgccat gttggcgacg tcctcgacct caccctggcg gggcagcggc gtgcacatcg cgtagtcgga gctcaactcc gcggactcgg tgatcgctgc gacgaggtcg gtgcggatca agccggggcg gatgctgttt acccgcaccc acgacgcgcc gagctcgtcg gcggccaact gcatcaggtg atcgaccgcg gacttggtga caccgtaggc accgaaccag cgatgggtat tgctggccgc aatcgaggag atgccgacaa acgatccgcc gccgcctcgc accatctctc gtgcggcgtg tttgaggacg tacatggtgc cgttgacgtt gaggtcgacc gtacggcgcc acgcttcgga gtcgacctgg gtgatcggtc cgatggtctc cgatccgccc gcacagtgca ccactccatg cagccggccg tgccaggcgg taaccgcatc caccgcgcgg gcggtctcgt cctcgttggt gatgtcggtg ggttcgtacc ggatggcgcc gccgttggca cctaacgctt cgagttcctg taccgcgccg gcgagcttgt cggggttacg gccgacgatc atgaccgagg ctccggccgc gaccagcccg gcggccactc cctttccgat tccgctaccg ccgccggtga ccaggtaggt ccggtcttgg aacgaaagct gcatcgaacc aggaccctgg gtctgagctt ccagggtacc
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMQLSFQDRT YLVTGGGSGI GKGVAAGLVA AGASVMIVGR NPDKLAGAVQ ELEALGANGG AIRYEPTDIT NEDETARAVD AVTAWHGRLH GVVHCAGGSE TIGPITQVDS EAWRRTVDLN VNGTMYVLKH AAREMVRGGG GSFVGISSIA ASNTHRWFGA YGVTKSAVDH LMQLAADELG ASWVRVNSIR PGLIRTDLVA AITESAELSS DYAMCTPLPR QGEVEDVANM AMFLLSDAAS FVTGQVINVD GGQMLRRGPD FSAMLEPVFG RDALRGL
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gcagctttcg ttccaagacc ggacctacct ggtcaccggc ggcggtagcg gaatcggaaa gggagtggcc gccgggctgg tcgcggccgg agcctcggtc atgatcgtcg gccgtaaccc cgacaagctc gccggcgcgg tacaggaact cgaagcgtta ggtgccaacg gcggcgccat ccggtacgaa cccaccgaca tcaccaacga ggacgagacc gcccgcgcgg tggatgcggt taccgcctgg cacggccggc tgcatggagt ggtgcactgt gcgggcggat cggagaccat cggaccgatc acccaggtcg actccgaagc gtggcgccgt acggtcgacc tcaacgtcaa cggcaccatg tacgtcctca aacacgccgc acgagagatg gtgcgaggcg gcggcggatc gtttgtcggc atctcctcga ttgcggccag caatacccat cgctggttcg gtgcctacgg tgtcaccaag tccgcggtcg atcacctgat gcagttggcc gccgacgagc tcggcgcgtc gtgggtgcgg gtaaacagca tccgccccgg cttgatccgc accgacctcg tcgcagcgat caccgagtcc gcggagttga gctccgacta cgcgatgtgc acgccgctgc cccgccaggg tgaggtcgag gacgtcgcca acatggcgat gtttctgctc agcgatgccg ccagcttcgt caccggacag gtcatcaatg tcgacggcgg ccagatgctg cggcgcggcc cggacttctc ggcgatgctg gaaccggtgt tcggccgtga cgcactgcgc ggattgaaac agcacgaaca agttctgcag ccaagcttct cgaggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaa
Details for MymaA.00414.a.A1.PS00811
PURIFICATION DATe: 10/15/2010
CONCENTRATION: 75.39mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 3
VIAL VOLUME: 100µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 92
Protocol Notes
notes unavailable
Validated AA Sequence
MQLSFQDRTY LVTGGGSGIG KGVAAGLVAA GASVMIVGRN PDKLAGAVQE LEALGANGGA IRYEPTDITN EDETARAVDA VTAWHGRLHG VVHCAGGSET IGPITQVDSE AWRRTVDLNV NGTMYVLKHA AREMVRGGGG SFVGISSIAA SNTHRWFGAY GVTKSAVDHL MQLAADELGA SWVRVNSIRP GLIRTDLVAA ITESAELSSD YAMCTPLPRQ GEVEDVANMA MFLLSDAASF VTGQVINVDG GQMLRRGPDF SAMLEPVFGR DALRGL
Validated NT Sequence
ttattacaat ccgcgcagtg cgtcacggcc gaacaccggt tccagcatcg ccgagaagtc cgggccgcgc cgcagcatct ggccgccgtc gacattgatg acctgtccgg tgacgaagct ggcggcatcg ctgagcagaa acatcgccat gttggcgacg tcctcgacct caccctggcg gggcagcggc gtgcacatcg cgtagtcgga gctcaactcc gcggactcgg tgatcgctgc gacgaggtcg gtgcggatca agccggggcg gatgctgttt acccgcaccc acgacgcgcc gagctcgtcg gcggccaact gcatcaggtg atcgaccgcg gacttggtga caccgtaggc accgaaccag cgatgggtat tgctggccgc aatcgaggag atgccgacaa acgatccgcc gccgcctcgc accatctctc gtgcggcgtg tttgaggacg tacatggtgc cgttgacgtt gaggtcgacc gtacggcgcc acgcttcgga gtcgacctgg gtgatcggtc cgatggtctc cgatccgccc gcacagtgca ccactccatg cagccggccg tgccaggcgg taaccgcatc caccgcgcgg gcggtctcgt cctcgttggt gatgtcggtg ggttcgtacc ggatggcgcc gccgttggca cctaacgctt cgagttcctg taccgcgccg gcgagcttgt cggggttacg gccgacgatc atgaccgagg ctccggccgc gaccagcccg gcggccactc cctttccgat tccgctaccg ccgccggtga ccaggtaggt ccggtcttgg aacgaaagct gcatcgaacc aggaccctgg gtctgagctt ccagggtacc
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMQLSFQDRT YLVTGGGSGI GKGVAAGLVA AGASVMIVGR NPDKLAGAVQ ELEALGANGG AIRYEPTDIT NEDETARAVD AVTAWHGRLH GVVHCAGGSE TIGPITQVDS EAWRRTVDLN VNGTMYVLKH AAREMVRGGG GSFVGISSIA ASNTHRWFGA YGVTKSAVDH LMQLAADELG ASWVRVNSIR PGLIRTDLVA AITESAELSS DYAMCTPLPR QGEVEDVANM AMFLLSDAAS FVTGQVINVD GGQMLRRGPD FSAMLEPVFG RDALRGL
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GCAGCTTTCG TTCCAAGACC GGACCTACCT GGTCACCGGC GGCGGTAGCG GAATCGGAAA GGGAGTGGCC GCCGGGCTGG TCGCGGCCGG AGCCTCGGTC ATGATCGTCG GCCGTAACCC CGACAAGCTC GCCGGCGCGG TACAGGAACT CGAAGCGTTA GGTGCCAACG GCGGCGCCAT CCGGTACGAA CCCACCGACA TCACCAACGA GGACGAGACC GCCCGCGCGG TGGATGCGGT TACCGCCTGG CACGGCCGGC TGCATGGAGT GGTGCACTGT GCGGGCGGAT CGGAGACCAT CGGACCGATC ACCCAGGTCG ACTCCGAAGC GTGGCGCCGT ACGGTCGACC TCAACGTCAA CGGCACCATG TACGTCCTCA AACACGCCGC ACGAGAGATG GTGCGAGGCG GCGGCGGATC GTTTGTCGGC ATCTCCTCGA TTGCGGCCAG CAATACCCAT CGCTGGTTCG GTGCCTACGG TGTCACCAAG TCCGCGGTCG ATCACCTGAT GCAGTTGGCC GCCGACGAGC TCGGCGCGTC GTGGGTGCGG GTAAACAGCA TCCGCCCCGG CTTGATCCGC ACCGACCTCG TCGCAGCGAT CACCGAGTCC GCGGAGTTGA GCTCCGACTA CGCGATGTGC ACGCCGCTGC CCCGCCAGGG TGAGGTCGAG GACGTCGCCA ACATGGCGAT GTTTCTGCTC AGCGATGCCG CCAGCTTCGT CACCGGACAG GTCATCAATG TCGACGGCGG CCAGATGCTG CGGCGCGGCC CGGACTTCTC GGCGATGCTG GAACCGGTGT TCGGCCGTGA CGCACTGCGC GGATTGAAAC AGCACGAACA AGTTCTGCAG CCAAGCTTCT CGAGGATCCG GCTGCTAACA AAGCCCGAAA GGAAGCTGAG TTGGCTGCTG CCACCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC TAAACGGGTC TTGAGGGGTT TTTTGCTGAA AGGAGGAACT ATATCCGGAT ATCCACAGGA CGGGTGTGGT CGCCATGATC GCGTAGTCGA TAGTGGCTCC AAGTAGCGAA GCGAGCAGGA CTGGGCGGCG GCCAAAGCGG TCGGACAGTG CTCCGAGAAC GGGTGCGCAT AGAAATTGCA TCAACGCATA TAGCGCTAGC AGCACGCCAT AGTGACTGGC GATGCTGTCG GAATGGACGA TATCCCGCAA GAGGCCCGGC AGTACCGGCA TAACCAAGCC TATGCCTACA GCATCCAGGG TGACGGTGCC GAGGATGACG ATGAGCGCAT TGTTAGATTT CATACACGGT GCCTGACTGC GTTAGCAATT TAACTGTGAT AAACTACCGC ATTAAAGCTT ATCGATGATA AGCTGTCAAA CATGAGAA