MymaA.00384.a

Cytoplasmic peptidase PepQ

CENTER ID: MymaA.00384.a
ORGANISM: Mycobacterium marinum ATCC BAA-535 / M
ASSOCIATED DISEASE: Cutaneous ulcerations, deep abscesses
CURRENT STATUS: expressed
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

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Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MymaA.00384.a.A1.GE30015 Full length( MymaA.00384.a ) 1 372
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|216594.6.peg.2331
RefSeq: YP_001850484.1
UniProt: B2HND1

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTHSQRRHNL KSKISAAGLD AMLVTDLINV RYLSGFSGSN GALLVFADER EAVLATDGRY RTQAAHQAPD LEVAIERAIG RHLSCRAADD GVGRLGFESH VVTVDGFDAL TGALADTNTE LVRASGTVEA LREIKDAGEL ALLRLACEAA DAALSDLIKR GGLRPGRTER EVARDLEGLM LDHGADAVSF ETIVAAGPNS AIPHHRPTDA VLAKGDFVKI DFGALVAGYH SDMTRTFVLD KAADWQLEIY ELVAAAQKAG REALSAGAEL RDVDGAARQM IVAAGHGDNF GHGLGHGVGL QIHEAPGIGA TSVGTLLAGS VVTVEPGVYL PGRGGVRIED TLVVPNGGPK TPRSAAQTPE LLTRFPKELT IL
NT Sequence
GTGACACATT CCCAGCGTCG ACACAACCTC AAATCCAAAA TCAGTGCCGC CGGACTGGAC GCGATGCTGG TCACCGATTT GATCAATGTG CGATATCTCT CCGGGTTCAG CGGATCCAAC GGTGCGTTGC TGGTATTCGC CGACGAGCGT GAAGCCGTGC TGGCCACCGA CGGTCGATAC CGCACGCAGG CCGCCCATCA GGCGCCGGAC CTCGAGGTGG CCATCGAACG CGCCATCGGG CGACACCTGA GCTGCCGGGC TGCCGATGAC GGTGTTGGCC GGCTGGGCTT CGAAAGCCAT GTGGTCACGG TGGACGGCTT TGATGCGCTC ACGGGTGCGC TGGCGGACAC CAACACCGAG TTGGTCCGCG CCTCCGGCAC CGTGGAGGCA CTGCGCGAGA TCAAAGATGC CGGTGAGCTT GCGTTATTGC GGCTGGCGTG CGAGGCGGCC GACGCCGCGC TGTCGGACTT GATTAAGCGC GGCGGGCTGC GGCCAGGCCG CACCGAGCGC GAGGTGGCCC GCGATCTCGA GGGCCTGATG CTCGACCATG GTGCCGACGC GGTGTCGTTC GAGACGATTG TGGCTGCCGG GCCGAACTCG GCCATCCCAC ACCACCGGCC CACCGACGCG GTGCTGGCCA AGGGTGATTT CGTCAAGATC GACTTCGGTG CGCTGGTTGC CGGCTATCAC TCGGACATGA CCCGCACCTT CGTGCTGGAC AAGGCGGCCG ACTGGCAGTT GGAGATCTAT GAGCTGGTCG CCGCGGCGCA AAAGGCCGGT CGAGAGGCGT TGTCTGCGGG CGCCGAGTTG CGCGACGTGG ATGGCGCCGC GCGACAGATG ATCGTCGCCG CGGGGCATGG AGATAACTTC GGTCACGGTC TGGGACACGG TGTTGGCCTG CAGATACACG AAGCACCGGG CATCGGGGCC ACATCCGTCG GTACACTACT GGCGGGCTCC GTGGTTACCG TGGAGCCCGG CGTTTATTTG CCCGGCCGCG GCGGTGTCCG CATCGAGGAC ACGTTGGTAG TGCCCAATGG GGGCCCGAAG ACACCGAGAA GCGCCGCGCA GACCCCGGAA TTGTTGACCC GGTTCCCGAA GGAACTGACC ATCTTG
Details for MymaA.00384.a.A1.GE30015
HARVESTED ON: 8/10/2010
SEQUENCED ON: 8/16/2010
EXPECTED MW: 41kDa
OBSERVED MW: 41kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 96
PERCENT COVERAGE: 76
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMVTHSQRRH NLKSKISAAG LDAMLVTDLI NVRYLSGFSG SNGALLVFAD EREAVLATDG RYRTQAAHQA PDLEVAIERA IGRHLSCRAA DDGVGRLGFE SHVVTVDGFD ALTGALADTN TELVRASGTV EALREIKDAG ELALLRLACE AADAALSDLI KRGGLRPGRT EREVARDLEG LMLDHGADAV SFETIVAAGP NSAIPHHRPT DAVLAKGDFV KIDFGALVAG YHSDMTRTFV LDKAADWQLE IYELVAAAQX AGREXCLRAP SCATXXAPRD R
Validated NT Sequence
ttgtnnnntt taagaaggag atataccatg gctcatcacc atcaccatca tatgggtacc ctggaagctc agacccaggg tcctggttcg atggtgacac attcccagcg tcgacacaac ctcaaatcca aaatcagtgc cgccggactg gacgcgatgc tggtcaccga tttgatcaat gtgcgatatc tctccgggtt cagcggatcc aacggtgcgt tgctggtatt cgccgacgag cgtgaagccg tgctggccac cgacggtcga taccgcacgc aggccgccca tcaggcgccg gacctcgagg tggccatcga acgcgccatc gggcgacacc tgagctgccg ggctgccgat gacggtgttg gccggctggg cttcgaaagc catgtggtca cggtggacgg ctttgatgcg ctcacgggtg cgctggcgga caccaacacc gagttggtcc gcgcctccgg caccgtggag gcactgcgcg agatcaaaga tgccggtgag cttgcgttat tgcggctggc gtgcgaggcg gccgacgccg cgctgtcgga cttgattaag cgcggcgggc tgcggccagg ccgcaccgag cgcgaggtgg cccgcgatct cgagggcctg atgctcgacc atggtgccga cgcggtgtcg ttcgagacga ttgtggctgc cgggccgaac tcggccatcc cacaccaccg gcccaccgac gcggtgctgg ccaagggtga tttcgtcaag atcgacttcg gtgcgctggt tgccggctat cactcggaca tgacccgcac cttcgtgctg gacaaggcgg ccgactggca gttggagatc tatgagctgg tcgccgcggc gcaaaangcc ggtcgagagn gttgtctgcg ggcgccnagt tgcgcgacgt gnntggcgcc gcgcgacaga tgatcgtcgc cgcggggnat gnanatannt tnnntcacng tctggncggt gttngncnnn anatncacga agcancgnna tngnnnnccn cntncnnnnn nnnnctactn nnnggntnnn nnnnnccgnn nnnccttnnn nngncnnnnc gnntcnnatc nnnnnncnnn nngnnnnnnn nngncnnnnn nnnnnnnnnn nnnnnnnnnn nnnntnnnnn nnnnnnnnnn nnnacnnnnn nnannnnnnn nna
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVTHSQRRH NLKSKISAAG LDAMLVTDLI NVRYLSGFSG SNGALLVFAD EREAVLATDG RYRTQAAHQA PDLEVAIERA IGRHLSCRAA DDGVGRLGFE SHVVTVDGFD ALTGALADTN TELVRASGTV EALREIKDAG ELALLRLACE AADAALSDLI KRGGLRPGRT EREVARDLEG LMLDHGADAV SFETIVAAGP NSAIPHHRPT DAVLAKGDFV KIDFGALVAG YHSDMTRTFV LDKAADWQLE IYELVAAAQK AGREALSAGA ELRDVDGAAR QMIVAAGHGD NFGHGLGHGV GLQIHEAPGI GATSVGTLLA GSVVTVEPGV YLPGRGGVRI EDTLVVPNGG PKTPRSAAQT PELLTRFPKE LTI
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtgacacat tcccagcgtc gacacaacct caaatccaaa atcagtgccg ccggactgga cgcgatgctg gtcaccgatt tgatcaatgt gcgatatctc tccgggttca gcggatccaa cggtgcgttg ctggtattcg ccgacgagcg tgaagccgtg ctggccaccg acggtcgata ccgcacgcag gccgcccatc aggcgccgga cctcgaggtg gccatcgaac gcgccatcgg gcgacacctg agctgccggg ctgccgatga cggtgttggc cggctgggct tcgaaagcca tgtggtcacg gtggacggct ttgatgcgct cacgggtgcg ctggcggaca ccaacaccga gttggtccgc gcctccggca ccgtggaggc actgcgcgag atcaaagatg ccggtgagct tgcgttattg cggctggcgt gcgaggcggc cgacgccgcg ctgtcggact tgattaagcg cggcgggctg cggccaggcc gcaccgagcg cgaggtggcc cgcgatctcg agggcctgat gctcgaccat ggtgccgacg cggtgtcgtt cgagacgatt gtggctgccg ggccgaactc ggccatccca caccaccggc ccaccgacgc ggtgctggcc aagggtgatt tcgtcaagat cgacttcggt gcgctggttg ccggctatca ctcggacatg acccgcacct tcgtgctgga caaggcggcc gactggcagt tggagatcta tgagctggtc gccgcggcgc aaaaggccgg tcgagaggcg ttgtctgcgg gcgccgagtt gcgcgacgtg gatggcgccg cgcgacagat gatcgtcgcc gcggggcatg gagataactt cggtcacggt ctgggacacg gtgttggcct gcagatacac gaagcaccgg gcatcggggc cacatccgtc ggtacactac tggcgggctc cgtggttacc gtggagcccg gcgtttattt gcccggccgc ggcggtgtcc gcatcgagga cacgttggta gtgcccaatg ggggcccgaa gacaccgaga agcgccgcgc agaccccgga attgttgacc cggttcccga aggaactgac catcaaacag cacgaacaag ttctgcagcc aagcttctcg aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaa