SSGCID: Target Page

MymaA.00358.d

Enoyl-CoA hydratase EchA13

CENTER ID:	MymaA.00358.d
ORGANISM:	Mycobacterium marinum ATCC BAA-535 / M
ASSOCIATED DISEASE:	Cutaneous ulcerations, deep abscesses
CURRENT STATUS:	purified
COMMUNITY REQUEST:	False
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIC

Ordering Clones & Proteins

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Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MymaA.00358.d.A1.GE29998	Full length( MymaA.00358.d )		1	316

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MymaA.00358.d.A1.PS00812	Full length( MymaA.00358.d )		1	316

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|216594.6.peg.3058
RefSeq:	YP_001851158.1
UniProt:	B2HE23

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MNVAGVSPVD RRSNGERAAP DFTYVRYETI DDGRIATITL NRPKQRNAQT RGMLVELGAA FELAEADDTV RVVILRGEGP AFSAGHDLGS ADDVRERAPG PDQHPTYQCN GGTYGGVESR NRQEWHYYFE NTKRWRNLRK ITVAQVHGTV LSAGLMLAWC CDLIVAADDT VFADVVGTRL GMCGVEYFGH PWEFGPRKAK ELLLTGDSIG ADEAHALGMV SKVFPGEDLA DSAIEFARRI AKLPTMAALL IKESVNQTVD AMGFTTALDG CFKIHQLNHA HWAEVTGGKL CYGTVEYGLD DWRAAPEILP ATKRRP

NT Sequence

ATGAACGTCG CCGGTGTCAG TCCAGTCGAT CGCCGGTCCA ACGGGGAGCG TGCGGCGCCG GACTTCACAT ACGTCCGGTA CGAAACGATC GACGACGGCC GTATCGCGAC GATCACCCTG AATCGTCCGA AACAGCGCAA CGCGCAAACG CGCGGAATGC TGGTGGAGTT GGGTGCCGCC TTCGAACTTG CCGAAGCTGA CGACACCGTC CGGGTGGTCA TCCTGCGCGG CGAGGGGCCC GCCTTTTCCG CCGGGCACGA CCTGGGCTCG GCCGATGATG TCCGGGAACG CGCCCCGGGG CCCGACCAGC ACCCCACCTA TCAGTGCAAT GGCGGCACCT ATGGCGGGGT GGAGTCACGC AACCGCCAGG AGTGGCACTA CTACTTCGAG AACACGAAGA GGTGGCGCAA TCTGCGCAAG ATCACCGTCG CCCAGGTGCA CGGGACGGTG CTGTCGGCGG GTTTGATGTT GGCCTGGTGC TGTGACTTGA TCGTCGCTGC CGATGACACC GTATTCGCCG ATGTCGTGGG CACCCGTCTC GGCATGTGTG GAGTCGAGTA CTTCGGCCAC CCGTGGGAGT TCGGCCCGCG CAAGGCCAAG GAGCTCTTGC TTACCGGCGA CTCGATCGGC GCGGACGAAG CCCACGCGTT GGGCATGGTG AGCAAGGTAT TTCCGGGCGA GGATCTAGCC GATAGCGCGA TCGAATTTGC GCGCCGCATC GCCAAGCTAC CGACGATGGC TGCGCTGCTC ATCAAGGAAT CGGTGAACCA GACCGTGGAC GCCATGGGTT TCACCACGGC GCTGGACGGG TGCTTCAAGA TCCATCAGCT CAATCATGCG CACTGGGCCG AGGTCACCGG CGGAAAGCTG TGCTACGGCA CCGTCGAGTA TGGACTCGAC GACTGGCGGG CCGCTCCGGA GATTCTGCCC GCGACCAAGC GGCGGCCC

Details for MymaA.00358.d.A1.GE29998

HARVESTED ON:	7/13/2010
SEQUENCED ON:	8/20/2010
EXPECTED MW:	36kDa
OBSERVED MW:	37kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Many (50-100)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	High Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	100
PERCENT COVERAGE:	97

Validated AA Sequence

MGTLEAQTQG PGSMNVAGVS PVDRRSNGER AAPDFTYVRY ETIDDGRIAT ITLNRPKQRN AQTRGMLVEL GAAFELAEAD DTVRVVILRG EGPAFSAGHD LGSADDVRER APGPDQHPTY QCNGGTYGGV ESRNRQEWHY YFENTKRWRN LRKITVAQVH GTVLSAGLML AWCCDLIVAA DDTVFADVVG TRLGMCGVEY FGHPWEFGPR KAKELLLTGD SIGADEAHAL GMVSKVFPGE DLADSAIEFA RRIAKLPTMA ALLIKESVNQ TVDAMGFTTA LDGCFKIHQL NHAHWAEVTG GKLCYGTVEY GLDDWRAAPE ILPATKRR

Validated NT Sequence

ttattaccgc cgcttggtcg cgggcagaat ctccggagcg gcccgccagt cgtcgagtcc atactcgacg gtgccgtagc acagctttcc gccggtgacc tcggcccagt gcgcatgatt gagctgatgg atcttgaagc acccgtccag cgccgtggtg aaacccatgg cgtccacggt ctggttcacc gattccttga tgagcagcgc agccatcgtc ggtagcttgg cgatgcggcg cgcaaattcg atcgcgctat cggctagatc ctcgcccgga aataccttgc tcaccatgcc caacgcgtgg gcttcgtccg cgccgatcga gtcgccggta agcaagagct ccttggcctt gcgcgggccg aactcccacg ggtggccgaa gtactcgact ccacacatgc cgagacgggt gcccacgaca tcggcgaata cggtgtcatc ggcagcgacg atcaagtcac agcaccaggc caacatcaaa cccgccgaca gcaccgtccc gtgcacctgg gcgacggtga tcttgcgcag attgcgccac ctcttcgtgt tctcgaagta gtagtgccac tcctggcggt tgcgtgactc caccccgcca taggtgccgc cattgcactg ataggtgggg tgctggtcgg gccccggggc gcgttcccgg acatcatcgg ccgagcccag gtcgtgcccg gcggaaaagg cgggcccctc gccgcgcagg atgaccaccc ggacggtgtc gtcagcttcg gcaagttcga aggcggcacc caactccacc agcattccgc gcgtttgcgc gttgcgctgt ttcggacgat tcagggtgat cgtcgcgata cggccgtcgt cgatcgtttc gtaccggacg tatgtgaagt ccggcgccgc acgctccccg ttggaccggc gatcgactgg actgacaccg gcgacgttca tcgaaccagg accctgggtc tgagcttcca gggtacccat atgatggtga tggtgatga

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMNVAGVSPV DRRSNGERAA PDFTYVRYET IDDGRIATIT LNRPKQRNAQ TRGMLVELGA AFELAEADDT VRVVILRGEG PAFSAGHDLG SADDVRERAP GPDQHPTYQC NGGTYGGVES RNRQEWHYYF ENTKRWRNLR KITVAQVHGT VLSAGLMLAW CCDLIVAADD TVFADVVGTR LGMCGVEYFG HPWEFGPRKA KELLLTGDSI GADEAHALGM VSKVFPGEDL ADSAIEFARR IAKLPTMAAL LIKESVNQTV DAMGFTTALD GCFKIHQLNH AHWAEVTGGK LCYGTVEYGL DDWRAAPEIL PATKRR

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gaacgtcgcc ggtgtcagtc cagtcgatcg ccggtccaac ggggagcgtg cggcgccgga cttcacatac gtccggtacg aaacgatcga cgacggccgt atcgcgacga tcaccctgaa tcgtccgaaa cagcgcaacg cgcaaacgcg cggaatgctg gtggagttgg gtgccgcctt cgaacttgcc gaagctgacg acaccgtccg ggtggtcatc ctgcgcggcg aggggcccgc cttttccgcc gggcacgacc tgggctcggc cgatgatgtc cgggaacgcg ccccggggcc cgaccagcac cccacctatc agtgcaatgg cggcacctat ggcggggtgg agtcacgcaa ccgccaggag tggcactact acttcgagaa cacgaagagg tggcgcaatc tgcgcaagat caccgtcgcc caggtgcacg ggacggtgct gtcggcgggt ttgatgttgg cctggtgctg tgacttgatc gtcgctgccg atgacaccgt attcgccgat gtcgtgggca cccgtctcgg catgtgtgga gtcgagtact tcggccaccc gtgggagttc ggcccgcgca aggccaagga gctcttgctt accggcgact cgatcggcgc ggacgaagcc cacgcgttgg gcatggtgag caaggtattt ccgggcgagg atctagccga tagcgcgatc gaatttgcgc gccgcatcgc caagctaccg acgatggctg cgctgctcat caaggaatcg gtgaaccaga ccgtggacgc catgggtttc accacggcgc tggacgggtg cttcaagatc catcagctca atcatgcgca ctgggccgag gtcaccggcg gaaagctgtg ctacggcacc gtcgagtatg gactcgacga ctggcgggcc gctccggaga ttctgcccgc gaccaagcgg cggaaacagc acgaacaagt tctgcagcca agcttctcga ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaa

Details for MymaA.00358.d.A1.PS00812

PURIFICATION DATe:	10/14/2010
CONCENTRATION:	20.23mg/ml
OBSERVED MW:	data unavailable
EXPRESSION LEVEL:	Moderate Expression
PROTEIN PURIFICATION BUFFER:	20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	3
VIAL VOLUME:	100µl
PERCENT IDENTITY:	100
PERCENT COVERAGE:	97

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GAACGTCGCC GGTGTCAGTC CAGTCGATCG CCGGTCCAAC GGGGAGCGTG CGGCGCCGGA CTTCACATAC GTCCGGTACG AAACGATCGA CGACGGCCGT ATCGCGACGA TCACCCTGAA TCGTCCGAAA CAGCGCAACG CGCAAACGCG CGGAATGCTG GTGGAGTTGG GTGCCGCCTT CGAACTTGCC GAAGCTGACG ACACCGTCCG GGTGGTCATC CTGCGCGGCG AGGGGCCCGC CTTTTCCGCC GGGCACGACC TGGGCTCGGC CGATGATGTC CGGGAACGCG CCCCGGGGCC CGACCAGCAC CCCACCTATC AGTGCAATGG CGGCACCTAT GGCGGGGTGG AGTCACGCAA CCGCCAGGAG TGGCACTACT ACTTCGAGAA CACGAAGAGG TGGCGCAATC TGCGCAAGAT CACCGTCGCC CAGGTGCACG GGACGGTGCT GTCGGCGGGT TTGATGTTGG CCTGGTGCTG TGACTTGATC GTCGCTGCCG ATGACACCGT ATTCGCCGAT GTCGTGGGCA CCCGTCTCGG CATGTGTGGA GTCGAGTACT TCGGCCACCC GTGGGAGTTC GGCCCGCGCA AGGCCAAGGA GCTCTTGCTT ACCGGCGACT CGATCGGCGC GGACGAAGCC CACGCGTTGG GCATGGTGAG CAAGGTATTT CCGGGCGAGG ATCTAGCCGA TAGCGCGATC GAATTTGCGC GCCGCATCGC CAAGCTACCG ACGATGGCTG CGCTGCTCAT CAAGGAATCG GTGAACCAGA CCGTGGACGC CATGGGTTTC ACCACGGCGC TGGACGGGTG CTTCAAGATC CATCAGCTCA ATCATGCGCA CTGGGCCGAG GTCACCGGCG GAAAGCTGTG CTACGGCACC GTCGAGTATG GACTCGACGA CTGGCGGGCC GCTCCGGAGA TTCTGCCCGC GACCAAGCGG CGGAAACAGC ACGAACAAGT TCTGCAGCCA AGCTTCTCGA GGATCCGGCT GCTAACAAAG CCCGAAAGGA AGCTGAGTTG GCTGCTGCCA CCGCTGAGCA ATAACTAGCA TAACCCCTTG GGGCCTCTAA ACGGGTCTTG AGGGGTTTTT TGCTGAAAGG AGGAACTATA TCCGGATATC CACAGGACGG GTGTGGTCGC CATGATCGCG TAGTCGATAG TGGCTCCAAG TAGCGAAGCG AGCAGGACTG GGCGGCGGCC AAAGCGGTCG GACAGTGCTC CGAGAACGGG TGCGCATAGA AATTGCATCA ACGCATATAG CGCTAGCAGC ACGCCATAGT GACTGGCGAT GCTGTCGGAA TGGACGATAT CCCGCAAGAG GCCCGGCAGT ACCGGCATAA CCAAGCCTAT GCCTACAGCA TCCAGGGTGA CGGTGCCGAG GATGACGATG AGCGCATTGT TAGATTTCAT ACACGGTGCC TGACTGCGTT AGCAATTTAA CTGTGATAAA CTACCGCATT AAAGCTTATC GATGATAAGC TGTCAAACAT GAGAA