MymaA.00337.a

Oxidoreductase

CENTER ID: MymaA.00337.a
ORGANISM: Mycobacterium marinum ATCC BAA-535 / M
ASSOCIATED DISEASE: Cutaneous ulcerations, deep abscesses
CURRENT STATUS: purified
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MymaA.00337.a.A1.GE29994 Full length( MymaA.00337.a ) 1 317
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MymaA.00337.a.A1.PW29862 Full length( MymaA.00337.a ) 1 317

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|216594.6.peg.3592
RefSeq: YP_001851636.1
UniProt: B2HHT3

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MASDLIATVP DLSGKLAIVT GANSGLGFGL ARRLSAAGAD VVMAIRNRAK GEAAIEEIRS AVPDAKLSIK ALDLSSLASV AALGDQLNSE GRPIDILINN AGVMTPPERD TTADGFELQF GSNHLGHFAL TAHVLPLLRA AQGARVVSLS SLAARRGRIH FDDLQFEKSY AAMTAYGQSK LAVLMFAREL DRRSRAAGWG VMSNAAHPGL TKTNLQISGP SHGREKPALM QRFYTTSWRF APFLWQEIED GILPALYAAV TPQAEGGAFY GPRGFYEAAG GGVRAAKVPA RAGNDADCQR LWEVSERLTG VGYPTSS
NT Sequence
ATGGCCAGCG ATCTCATCGC CACCGTGCCC GATCTGTCCG GAAAACTCGC GATAGTCACC GGAGCCAATA GCGGGTTGGG TTTTGGGCTG GCCCGGCGGC TGTCGGCGGC GGGCGCCGAT GTGGTGATGG CAATCCGTAA CCGCGCCAAG GGCGAGGCGG CGATCGAGGA GATCCGCAGT GCCGTGCCCG ATGCCAAGTT GAGTATCAAG GCGCTCGATC TGTCCTCGCT GGCGTCGGTA GCCGCATTGG GTGACCAGCT CAACTCCGAG GGCCGCCCGA TCGACATCCT GATCAACAAC GCCGGCGTCA TGACTCCGCC CGAGCGTGAT ACCACCGCCG ACGGCTTCGA ATTGCAGTTC GGCAGTAACC ATCTCGGACA CTTCGCGCTG ACCGCGCACG TGCTGCCGCT GCTGCGCGCG GCGCAAGGCG CGCGGGTGGT ATCGCTGAGC AGCCTGGCGG CCCGGCGTGG CCGTATCCAC TTTGACGACC TGCAGTTCGA GAAGTCATAC GCTGCGATGA CGGCATACGG TCAATCCAAG CTGGCGGTGC TGATGTTCGC GCGCGAGCTG GACCGTCGCA GCCGGGCGGC GGGTTGGGGC GTGATGTCCA ACGCGGCGCA CCCAGGACTG ACCAAGACCA ACCTGCAGAT CAGCGGGCCT TCGCATGGGC GGGAGAAGCC GGCACTGATG CAGCGGTTCT ACACCACCTC ATGGCGTTTC GCTCCGTTCC TTTGGCAAGA AATCGAAGAC GGGATCCTGC CGGCGCTCTA CGCCGCCGTT ACACCGCAGG CCGAGGGCGG TGCTTTCTAC GGCCCGCGGG GCTTCTATGA GGCGGCCGGT GGCGGTGTGA GGGCGGCCAA GGTGCCCGCT CGTGCTGGCA ATGACGCTGA CTGCCAACGC CTTTGGGAGG TTTCCGAACG TCTCACGGGT GTCGGTTACC CCACGTCGAG C
Details for MymaA.00337.a.A1.GE29994
HARVESTED ON: 7/13/2010
SEQUENCED ON: 8/18/2010
EXPECTED MW: 35kDa
OBSERVED MW: 36kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMASDLIATV PDLSGKLAIV TGANSGLGFG LARRLSAAGA DVVMAIRNRA KGEAAIEEIR SAVPDAKLSI KALDLSSLAS VAALGDQLNS EGRPIDILIN NAGVMTPPER DTTADGFELQ FGSNHLGHFA LTAHVLPLLR AAQGARVVSL SSLAARRGRI HFDDLQFEKS YAAMTAYGQS KLAVLMFARE LDRRSRAAGW GVMSNAAHPG LTKTNLQISG PSHGREKPAL MQRFYTTSWR FAPFLWQEIE DGILPALYAA VTPQAEGGAF YGPRGFYEAA GGGVRAAKVP ARAGNDADCQ RLWEVSERLT GVGYPTS
Validated NT Sequence
ttattacgac gtggggtaac cgacacccgt gagacgttcg gaaacctccc aaaggcgttg gcagtcagcg tcattgccag cacgagcggg caccttggcc gccctcacac cgccaccggc cgcctcatag aagccccgcg ggccgtagaa agcaccgccc tcggcctgcg gtgtaacggc ggcgtagagc gccggcagga tcccgtcttc gatttcttgc caaaggaacg gagcgaaacg ccatgaggtg gtgtagaacc gctgcatcag tgccggcttc tcccgcccat gcgaaggccc gctgatctgc aggttggtct tggtcagtcc tgggtgcgcc gcgttggaca tcacgcccca acccgccgcc cggctgcgac ggtccagctc gcgcgcgaac atcagcaccg ccagcttgga ttgaccgtat gccgtcatcg cagcgtatga cttctcgaac tgcaggtcgt caaagtggat acggccacgc cgggccgcca ggctgctcag cgataccacc cgcgcgcctt gcgccgcgcg cagcagcggc agcacgtgcg cggtcagcgc gaagtgtccg agatggttac tgccgaactg caattcgaag ccgtcggcgg tggtatcacg ctcgggcgga gtcatgacgc cggcgttgtt gatcaggatg tcgatcgggc ggccctcgga gttgagctgg tcacccaatg cggctaccga cgccagcgag gacagatcga gcgccttgat actcaacttg gcatcgggca cggcactgcg gatctcctcg atcgccgcct cgcccttggc gcggttacgg attgccatca ccacatcggc gcccgccgcc gacagccgcc gggccagccc aaaacccaac ccgctattgg ctccggtgac tatcgcgagt tttccggaca gatcgggcac ggtggcgatg agatcgctgg ccatcgaacc aggaccctgg gtctgagctt ccagggtacc catatgatgg tgatggtgat gagccatggt at
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMASDLIATV PDLSGKLAIV TGANSGLGFG LARRLSAAGA DVVMAIRNRA KGEAAIEEIR SAVPDAKLSI KALDLSSLAS VAALGDQLNS EGRPIDILIN NAGVMTPPER DTTADGFELQ FGSNHLGHFA LTAHVLPLLR AAQGARVVSL SSLAARRGRI HFDDLQFEKS YAAMTAYGQS KLAVLMFARE LDRRSRAAGW GVMSNAAHPG LTKTNLQISG PSHGREKPAL MQRFYTTSWR FAPFLWQEIE DGILPALYAA VTPQAEGGAF YGPRGFYEAA GGGVRAAKVP ARAGNDADCQ RLWEVSERLT GVGYPTS
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggccagcgat ctcatcgcca ccgtgcccga tctgtccgga aaactcgcga tagtcaccgg agccaatagc gggttgggtt ttgggctggc ccggcggctg tcggcggcgg gcgccgatgt ggtgatggca atccgtaacc gcgccaaggg cgaggcggcg atcgaggaga tccgcagtgc cgtgcccgat gccaagttga gtatcaaggc gctcgatctg tcctcgctgg cgtcggtagc cgcattgggt gaccagctca actccgaggg ccgcccgatc gacatcctga tcaacaacgc cggcgtcatg actccgcccg agcgtgatac caccgccgac ggcttcgaat tgcagttcgg cagtaaccat ctcggacact tcgcgctgac cgcgcacgtg ctgccgctgc tgcgcgcggc gcaaggcgcg cgggtggtat cgctgagcag cctggcggcc cggcgtggcc gtatccactt tgacgacctg cagttcgaga agtcatacgc tgcgatgacg gcatacggtc aatccaagct ggcggtgctg atgttcgcgc gcgagctgga ccgtcgcagc cgggcggcgg gttggggcgt gatgtccaac gcggcgcacc caggactgac caagaccaac ctgcagatca gcgggccttc gcatgggcgg gagaagccgg cactgatgca gcggttctac accacctcat ggcgtttcgc tccgttcctt tggcaagaaa tcgaagacgg gatcctgccg gcgctctacg ccgccgttac accgcaggcc gagggcggtg ctttctacgg cccgcggggc ttctatgagg cggccggtgg cggtgtgagg gcggccaagg tgcccgctcg tgctggcaat gacgctgact gccaacgcct ttgggaggtt tccgaacgtc tcacgggtgt cggttacccc acgtcgaaac agcacgaaca agttctgcag ccaagcttct cgaggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaa
Details for MymaA.00337.a.A1.PW29862
PURIFICATION DATe: 11/15/2010
CONCENTRATION: 23.73mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 1
VIAL VOLUME: 100µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMASDLIATV PDLSGKLAIV TGANSGLGFG LARRLSAAGA DVVMAIRNRA KGEAAIEEIR SAVPDAKLSI KALDLSSLAS VAALGDQLNS EGRPIDILIN NAGVMTPPER DTTADGFELQ FGSNHLGHFA LTAHVLPLLR AAQGARVVSL SSLAARRGRI HFDDLQFEKS YAAMTAYGQS KLAVLMFARE LDRRSRAAGW GVMSNAAHPG LTKTNLQISG PSHGREKPAL MQRFYTTSWR FAPFLWQEIE DGILPALYAA VTPQAEGGAF YGPRGFYEAA GGGVRAAKVP ARAGNDADCQ RLWEVSERLT GVGYPTS
Validated NT Sequence
ttattacgac gtggggtaac cgacacccgt gagacgttcg gaaacctccc aaaggcgttg gcagtcagcg tcattgccag cacgagcggg caccttggcc gccctcacac cgccaccggc cgcctcatag aagccccgcg ggccgtagaa agcaccgccc tcggcctgcg gtgtaacggc ggcgtagagc gccggcagga tcccgtcttc gatttcttgc caaaggaacg gagcgaaacg ccatgaggtg gtgtagaacc gctgcatcag tgccggcttc tcccgcccat gcgaaggccc gctgatctgc aggttggtct tggtcagtcc tgggtgcgcc gcgttggaca tcacgcccca acccgccgcc cggctgcgac ggtccagctc gcgcgcgaac atcagcaccg ccagcttgga ttgaccgtat gccgtcatcg cagcgtatga cttctcgaac tgcaggtcgt caaagtggat acggccacgc cgggccgcca ggctgctcag cgataccacc cgcgcgcctt gcgccgcgcg cagcagcggc agcacgtgcg cggtcagcgc gaagtgtccg agatggttac tgccgaactg caattcgaag ccgtcggcgg tggtatcacg ctcgggcgga gtcatgacgc cggcgttgtt gatcaggatg tcgatcgggc ggccctcgga gttgagctgg tcacccaatg cggctaccga cgccagcgag gacagatcga gcgccttgat actcaacttg gcatcgggca cggcactgcg gatctcctcg atcgccgcct cgcccttggc gcggttacgg attgccatca ccacatcggc gcccgccgcc gacagccgcc gggccagccc aaaacccaac ccgctattgg ctccggtgac tatcgcgagt tttccggaca gatcgggcac ggtggcgatg agatcgctgg ccatcgaacc aggaccctgg gtctgagctt ccagggtacc catatgatgg tgatggtgat gagccatggt at
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMASDLIATV PDLSGKLAIV TGANSGLGFG LARRLSAAGA DVVMAIRNRA KGEAAIEEIR SAVPDAKLSI KALDLSSLAS VAALGDQLNS EGRPIDILIN NAGVMTPPER DTTADGFELQ FGSNHLGHFA LTAHVLPLLR AAQGARVVSL SSLAARRGRI HFDDLQFEKS YAAMTAYGQS KLAVLMFARE LDRRSRAAGW GVMSNAAHPG LTKTNLQISG PSHGREKPAL MQRFYTTSWR FAPFLWQEIE DGILPALYAA VTPQAEGGAF YGPRGFYEAA GGGVRAAKVP ARAGNDADCQ RLWEVSERLT GVGYPTS
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGCCAGCGAT CTCATCGCCA CCGTGCCCGA TCTGTCCGGA AAACTCGCGA TAGTCACCGG AGCCAATAGC GGGTTGGGTT TTGGGCTGGC CCGGCGGCTG TCGGCGGCGG GCGCCGATGT GGTGATGGCA ATCCGTAACC GCGCCAAGGG CGAGGCGGCG ATCGAGGAGA TCCGCAGTGC CGTGCCCGAT GCCAAGTTGA GTATCAAGGC GCTCGATCTG TCCTCGCTGG CGTCGGTAGC CGCATTGGGT GACCAGCTCA ACTCCGAGGG CCGCCCGATC GACATCCTGA TCAACAACGC CGGCGTCATG ACTCCGCCCG AGCGTGATAC CACCGCCGAC GGCTTCGAAT TGCAGTTCGG CAGTAACCAT CTCGGACACT TCGCGCTGAC CGCGCACGTG CTGCCGCTGC TGCGCGCGGC GCAAGGCGCG CGGGTGGTAT CGCTGAGCAG CCTGGCGGCC CGGCGTGGCC GTATCCACTT TGACGACCTG CAGTTCGAGA AGTCATACGC TGCGATGACG GCATACGGTC AATCCAAGCT GGCGGTGCTG ATGTTCGCGC GCGAGCTGGA CCGTCGCAGC CGGGCGGCGG GTTGGGGCGT GATGTCCAAC GCGGCGCACC CAGGACTGAC CAAGACCAAC CTGCAGATCA GCGGGCCTTC GCATGGGCGG GAGAAGCCGG CACTGATGCA GCGGTTCTAC ACCACCTCAT GGCGTTTCGC TCCGTTCCTT TGGCAAGAAA TCGAAGACGG GATCCTGCCG GCGCTCTACG CCGCCGTTAC ACCGCAGGCC GAGGGCGGTG CTTTCTACGG CCCGCGGGGC TTCTATGAGG CGGCCGGTGG CGGTGTGAGG GCGGCCAAGG TGCCCGCTCG TGCTGGCAAT GACGCTGACT GCCAACGCCT TTGGGAGGTT TCCGAACGTC TCACGGGTGT CGGTTACCCC ACGTCGAAAC AGCACGAACA AGTTCTGCAG CCAAGCTTCT CGAGGATCCG GCTGCTAACA AAGCCCGAAA GGAAGCTGAG TTGGCTGCTG CCACCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC TAAACGGGTC TTGAGGGGTT TTTTGCTGAA AGGAGGAACT ATATCCGGAT ATCCACAGGA CGGGTGTGGT CGCCATGATC GCGTAGTCGA TAGTGGCTCC AAGTAGCGAA GCGAGCAGGA CTGGGCGGCG GCCAAAGCGG TCGGACAGTG CTCCGAGAAC GGGTGCGCAT AGAAATTGCA TCAACGCATA TAGCGCTAGC AGCACGCCAT AGTGACTGGC GATGCTGTCG GAATGGACGA TATCCCGCAA GAGGCCCGGC AGTACCGGCA TAACCAAGCC TATGCCTACA GCATCCAGGG TGACGGTGCC GAGGATGACG ATGAGCGCAT TGTTAGATTT CATACACGGT GCCTGACTGC GTTAGCAATT TAACTGTGAT AAACTACCGC ATTAAAGCTT ATCGATGATA AGCTGTCAAA CATGAGAA