MymaA.00309.c

Acyl-CoA dehydrogenase FadE1

CENTER ID: MymaA.00309.c
ORGANISM: Mycobacterium marinum ATCC BAA-535 / M
ASSOCIATED DISEASE: Cutaneous ulcerations, deep abscesses
CURRENT STATUS: soluble
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MymaA.00309.c.A1.GE29992 Full length( MymaA.00309.c ) 1 431
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|216594.6.peg.369
RefSeq: YP_001848653.1
UniProt: B2HLP9

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MWDFETDPEY QAKLDWVQEF MVNELEPLDL VALDPYDKKN PEMMAVLRPL QQQVKDQGLW AAHLRPELGG QGFGQVKLAL LNEILGRSRW APSVFGCQAP DSGNAEILAL FGTEDQKARY LQPLLDGEIT SCYSMTEPQG GSDPGSFVTA ATRDGDSWII NGEKWFSTNA KHASFFIVMA VTNPQARTYE KMSLFIVPAE TPGIEIVRNV GVGTESSKHA SHGYVRYHDV RVPADHVLGG EGQAFMIAQT RLGGGRIHHA MRTIALARRA FDMMCERAVS RQTRHGRLSD LQMTQEKVAD SWIQIEQFRL LVLRTAWLID KHHDYQKVRR DIAAVKVAMP QVLHDVVQRA MHLHGALGVS DEMPFVKMMV AAESLGIADG ATELHKMTVA RRTLRQYEPV TTPFPSQHIP TRRAAAEARL AERLEHSIAE F
NT Sequence
GTGTGGGACT TCGAAACAGA CCCCGAATAC CAGGCGAAGC TGGACTGGGT CCAAGAGTTC ATGGTCAACG AACTCGAACC ACTGGATCTG GTAGCTCTCG ATCCCTACGA CAAGAAGAAC CCCGAGATGA TGGCCGTCCT TCGGCCGTTG CAGCAGCAGG TGAAGGACCA GGGGCTCTGG GCCGCTCACC TGCGACCGGA ACTCGGCGGG CAGGGGTTTG GCCAAGTCAA GCTTGCGCTG CTGAACGAAA TCCTCGGACG CTCGCGCTGG GCGCCCTCGG TGTTCGGTTG TCAGGCACCA GACTCGGGTA ACGCCGAGAT CCTGGCGCTG TTCGGCACCG AGGACCAGAA GGCCCGCTAC CTGCAACCGC TGCTGGACGG CGAAATCACC TCCTGCTACT CGATGACAGA ACCTCAGGGA GGTTCGGATC CAGGGTCGTT CGTCACGGCC GCCACCCGCG ACGGAGACAG CTGGATCATC AACGGGGAGA AGTGGTTTTC CACCAACGCC AAGCACGCCT CGTTCTTCAT CGTGATGGCC GTGACCAACC CGCAGGCCCG CACCTACGAG AAGATGTCGC TGTTCATCGT CCCTGCGGAG ACACCCGGTA TCGAGATCGT CCGCAACGTC GGCGTCGGGA CCGAGTCGAG CAAGCACGCC AGCCACGGCT ACGTCCGCTA CCACGATGTT CGGGTGCCGG CCGATCATGT GCTCGGCGGT GAAGGCCAGG CGTTCATGAT CGCGCAGACG CGACTCGGCG GCGGTCGCAT TCACCACGCG ATGCGCACAA TTGCATTGGC ACGACGAGCC TTTGACATGA TGTGCGAACG CGCCGTATCC AGGCAGACCA GACACGGCCG GCTTTCCGAT TTGCAGATGA CGCAGGAGAA GGTCGCCGAC AGCTGGATCC AGATCGAGCA GTTCCGGCTA CTGGTGCTGC GCACGGCGTG GCTGATCGAC AAGCACCACG ACTATCAGAA GGTGCGTCGC GACATCGCGG CGGTGAAGGT CGCAATGCCA CAGGTGCTGC ACGACGTGGT GCAGCGCGCC ATGCACCTGC ACGGCGCGCT CGGCGTTTCC GACGAGATGC CGTTCGTCAA GATGATGGTG GCCGCCGAAT CGCTGGGCAT CGCCGACGGC GCCACCGAAC TGCACAAGAT GACGGTGGCA CGCCGGACAC TGCGCCAATA CGAGCCTGTT ACAACGCCGT TCCCGTCACA GCACATACCC ACCCGGCGAG CGGCGGCCGA AGCGCGGCTG GCCGAGCGCC TGGAACACAG CATCGCGGAG TTC
Details for MymaA.00309.c.A1.GE29992
HARVESTED ON: 7/13/2010
SEQUENCED ON: 8/18/2010
EXPECTED MW: 50kDa
OBSERVED MW: 48kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 95
Validated AA Sequence
MVWDFETDPE YQAKLDWVQE FMVNELEPLD LVALDPYDKK NPEMMAVLRP LQQQVKDQGL WAAHLRPELG GQGFGQVKLA LLNEILGRSR WAPSVFGCQA PDSGNAEILA LFGTEDQKAR YLQPLLDGEI TSCYSMTEPQ GGSDPGSFVT AATRDGDSWI INGEKWFSTN AKHASFFIVM AVTNPQARTY EKMSLFIVPA ETPGIEIVRN VGVGTESSKH ASHGYVRYHD VRVPADHVLG GEGQAFMIAQ TRLGGGRIHH AMRTIALARR AFDMMCERAV SRQTRHGRLS DLQMTQEKVA DSWIQIEQFR LLVLRTAWLI DKHHDYQKVR RDIAAVKVAM PQVLHDVVQR AMHLHGALGV SDEMPFVKMM VAAESLGIAD GATELHKMTV ARRTLRQYEP VTTPFPSQHI PTRRAAAEAR LAERLEHSIA E
Validated NT Sequence
ttattactcc gcgatgctgt gttccaggcg ctcggccagc cgcgcttcgg ccgccgctcg ccgggtgggt atgtgctgtg acgggaacgg cgttgtaaca ggctcgtatt ggcgcagtgt ccggcgtgcc accgtcatct tgtgcagttc ggtggcgccg tcggcgatgc ccagcgattc ggcggccacc atcatcttga cgaacggcat ctcgtcggaa acgccgagcg cgccgtgcag gtgcatggcg cgctgcacca cgtcgtgcag cacctgtggc attgcgacct tcaccgccgc gatgtcgcga cgcaccttct gatagtcgtg gtgcttgtcg atcagccacg ccgtgcgcag caccagtagc cggaactgct cgatctggat ccagctgtcg gcgaccttct cctgcgtcat ctgcaaatcg gaaagccggc cgtgtctggt ctgcctggat acggcgcgtt cgcacatcat gtcaaaggct cgtcgtgcca atgcaattgt gcgcatcgcg tggtgaatgc gaccgccgcc gagtcgcgtc tgcgcgatca tgaacgcctg gccttcaccg ccgagcacat gatcggccgg cacccgaaca tcgtggtagc ggacgtagcc gtggctggcg tgcttgctcg actcggtccc gacgccgacg ttgcggacga tctcgatacc gggtgtctcc gcagggacga tgaacagcga catcttctcg taggtgcggg cctgcgggtt ggtcacggcc atcacgatga agaacgaggc gtgcttggcg ttggtggaaa accacttctc cccgttgatg atccagctgt ctccgtcgcg ggtggcggcc gtgacgaacg accctggatc cgaacctccc tgaggttctg tcatcgagta gcaggaggtg atttcgccgt ccagcagcgg ttgcaggtag cgggccttct ggtcctcggt gccgaacagc gccaggatct cggcgttacc cgagtctggt gcctgacaac cgaacaccga gggcgcccag cgcgagcgtc cgaggatttc gttcagcagc gcaagcttga cttggccaaa cccctgcccg ccgagttccg gtcgcaggtg agcggcccag agcccctggt ccttcacctg ctgctgcaac ggccgaagga cggccatcat ctcggggttc ttcttgtcgt agggatcgag agctaccaga tccagtggtt cgagttcgtt gaccatgaac tcttggaccc agtccagctt cgcctggtat tcggggtctg tttcgaagtc ccacaccatc gaaccaggac cctgggtctg agcttccagg gtac
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVWDFETDP EYQAKLDWVQ EFMVNELEPL DLVALDPYDK KNPEMMAVLR PLQQQVKDQG LWAAHLRPEL GGQGFGQVKL ALLNEILGRS RWAPSVFGCQ APDSGNAEIL ALFGTEDQKA RYLQPLLDGE ITSCYSMTEP QGGSDPGSFV TAATRDGDSW IINGEKWFST NAKHASFFIV MAVTNPQART YEKMSLFIVP AETPGIEIVR NVGVGTESSK HASHGYVRYH DVRVPADHVL GGEGQAFMIA QTRLGGGRIH HAMRTIALAR RAFDMMCERA VSRQTRHGRL SDLQMTQEKV ADSWIQIEQF RLLVLRTAWL IDKHHDYQKV RRDIAAVKVA MPQVLHDVVQ RAMHLHGALG VSDEMPFVKM MVAAESLGIA DGATELHKMT VARRTLRQYE PVTTPFPSQH IPTRRAAAEA RLAERLEHSI AE
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtgtgggac ttcgaaacag accccgaata ccaggcgaag ctggactggg tccaagagtt catggtcaac gaactcgaac cactggatct ggtagctctc gatccctacg acaagaagaa ccccgagatg atggccgtcc ttcggccgtt gcagcagcag gtgaaggacc aggggctctg ggccgctcac ctgcgaccgg aactcggcgg gcaggggttt ggccaagtca agcttgcgct gctgaacgaa atcctcggac gctcgcgctg ggcgccctcg gtgttcggtt gtcaggcacc agactcgggt aacgccgaga tcctggcgct gttcggcacc gaggaccaga aggcccgcta cctgcaaccg ctgctggacg gcgaaatcac ctcctgctac tcgatgacag aacctcaggg aggttcggat ccagggtcgt tcgtcacggc cgccacccgc gacggagaca gctggatcat caacggggag aagtggtttt ccaccaacgc caagcacgcc tcgttcttca tcgtgatggc cgtgaccaac ccgcaggccc gcacctacga gaagatgtcg ctgttcatcg tccctgcgga gacacccggt atcgagatcg tccgcaacgt cggcgtcggg accgagtcga gcaagcacgc cagccacggc tacgtccgct accacgatgt tcgggtgccg gccgatcatg tgctcggcgg tgaaggccag gcgttcatga tcgcgcagac gcgactcggc ggcggtcgca ttcaccacgc gatgcgcaca attgcattgg cacgacgagc ctttgacatg atgtgcgaac gcgccgtatc caggcagacc agacacggcc ggctttccga tttgcagatg acgcaggaga aggtcgccga cagctggatc cagatcgagc agttccggct actggtgctg cgcacggcgt ggctgatcga caagcaccac gactatcaga aggtgcgtcg cgacatcgcg gcggtgaagg tcgcaatgcc acaggtgctg cacgacgtgg tgcagcgcgc catgcacctg cacggcgcgc tcggcgtttc cgacgagatg ccgttcgtca agatgatggt ggccgccgaa tcgctgggca tcgccgacgg cgccaccgaa ctgcacaaga tgacggtggc acgccggaca ctgcgccaat acgagcctgt tacaacgccg ttcccgtcac agcacatacc cacccggcga gcggcggccg aagcgcggct ggccgagcgc ctggaacaca gcatcgcgga gaaacagcac gaacaagttc tgcagccaag cttctcgagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaa