SSGCID: Target Page

MymaA.00309.b

Acyl-CoA dehydrogenase FadE1_1

CENTER ID:	MymaA.00309.b
ORGANISM:	Mycobacterium marinum ATCC BAA-535 / M
ASSOCIATED DISEASE:	Cutaneous ulcerations, deep abscesses
CURRENT STATUS:	diffraction
COMMUNITY REQUEST:	False
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MymaA.00309.b.A1.GE29991	Full length( MymaA.00309.b )		1	435

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MymaA.00309.b.A1.PS00904	Full length( MymaA.00309.b )		1	435

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|216594.6.peg.5696
RefSeq:	YP_001853570.1
UniProt:	B2HLI3

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MPWDFSTEPE FEKKLDWIRE FVREEVEPLE VVFPGCEFLP LNDERRRIVD PLKQRVRDEG LWAPHLGPEL GGQGFGAVKL TLINEILGRS PWAPIVFGTQ APDTGNAEII ARFGTQDQKE RYLQGLLSGE IFSCFSMTEP QGGADPRVFT TRAVRDGDDW VISGRKYFSS NASVASFFIV VAITNPDVAV HHGASTFLVP AGIKGLTIEA NHHLVGALPH EPGHSLVHYD DVRVGADALL GEPGQGFLIL QTRLAGGRLH HAMRSIGVAQ RAVEMMSRRA KSRFTQGSSL ADKQLVQAFI ADSYTELMPF RLAVLHAAWM IDTAGEHAAR AEIGACKILA SQVLKSIGLR AIQVHGALGT TDQLPLVSVL LGGVALGLAD GPTEAHKVNL ARMLLKNYEP EDPQWPSESL TVRQAAARAK YGQLVDRVPA LPDSP

NT Sequence

ATGCCTTGGG ACTTCTCCAC CGAACCGGAG TTCGAGAAGA AGCTCGACTG GATCCGCGAG TTCGTACGCG AGGAGGTGGA GCCGCTCGAG GTGGTGTTCC CCGGCTGCGA GTTCCTGCCG CTCAACGATG AACGCCGCCG CATCGTGGAT CCACTCAAAC AACGAGTTCG CGACGAAGGC TTGTGGGCCC CACATTTGGG TCCGGAACTC GGCGGGCAGG GCTTTGGCGC GGTGAAACTG ACCCTGATCA ACGAGATCCT GGGACGCAGC CCCTGGGCTC CGATCGTGTT CGGCACCCAG GCACCCGACA CCGGCAACGC GGAGATCATT GCGCGCTTTG GCACCCAGGA TCAAAAGGAG CGCTACCTGC AGGGCTTGCT GTCCGGCGAG ATCTTCTCCT GCTTCTCCAT GACCGAGCCG CAGGGCGGTG CCGATCCGCG CGTCTTCACC ACCCGCGCCG TCCGCGACGG TGATGATTGG GTGATCAGCG GCCGCAAGTA TTTCTCGTCC AACGCCTCGG TCGCATCGTT TTTCATCGTC GTCGCGATCA CCAATCCGGA TGTGGCCGTT CACCACGGAG CGTCGACGTT CCTGGTCCCG GCTGGAATCA AGGGCTTGAC CATCGAAGCC AACCACCACC TGGTCGGCGC CCTGCCACAC GAACCGGGTC ACTCCCTGGT GCACTACGAC GACGTTCGAG TCGGTGCGGA CGCGCTGCTT GGGGAACCGG GTCAGGGATT TCTGATCCTG CAAACCCGCC TGGCCGGTGG CCGGCTGCAT CACGCGATGC GCTCCATCGG GGTGGCGCAG CGTGCGGTCG AGATGATGTC ACGCCGAGCG AAAAGTCGAT TCACCCAGGG CAGTTCGCTG GCCGATAAGC AGTTGGTGCA AGCGTTCATC GCCGACTCCT ATACCGAGCT GATGCCGTTC CGGCTGGCGG TGCTGCACGC GGCCTGGATG ATCGACACCG CCGGCGAGCA CGCGGCGCGC GCCGAGATCG GCGCCTGCAA GATCCTGGCA TCCCAAGTGC TCAAATCGAT TGGTCTGCGC GCTATCCAGG TGCACGGCGC GCTGGGCACC ACCGATCAGC TACCGCTGGT CAGCGTGCTA CTCGGCGGTG TCGCGCTGGG GCTGGCAGAC GGCCCCACCG AAGCTCACAA GGTGAACCTG GCGCGGATGC TGCTGAAGAA CTACGAGCCC GAAGACCCGC AGTGGCCCAG CGAATCACTG ACCGTCCGGC AAGCGGCCGC CCGCGCCAAG TACGGCCAAC TCGTCGATCG TGTTCCGGCC CTTCCCGATT CACCG

Details for MymaA.00309.b.A1.GE29991

HARVESTED ON:	7/13/2010
SEQUENCED ON:	8/18/2010
EXPECTED MW:	49kDa
OBSERVED MW:	48kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	High Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	99
PERCENT COVERAGE:	97

Validated AA Sequence

MGTLEAQTQG PGSMPWDFST EPEFEKKLDW IREFVREEVE PLEVVFPGCE FLPLNDERRR IVDPLXQRVR DEGLWAPHLG PELGGQGFGA VKLTLINEIL GRSPWAPIVF GTQAPDTGNA EIIARFGTQD QKERYLQGLL SGEIFSCFSM TEPQGGADPR VFTTRAVRDG DDWVISGRKY FSSNASVASF FIVVAITNPD VAVHHGASTF LVPAGIKGLT IEANHHLVGA LPHEPGHSLV HYDDVRVGAD ALLGEPGQGF LILQTRLAGG RLHHAMRSIG VAQRAVEMMS RRAKSRFTQG SSLADKQLVQ AFIADSYTEL MPFRLAVLHA AWMIDTAGEH AARAEIGACK ILASQVLKSI GLRAIQVHGA LGTTDQLPLV SVLLGGVALG LADGPTEAHK VNLARMLLKN YEPEDPQWPS ESLTVRQAAA RAKYGQLVDR VPALP

Validated NT Sequence

cgggaagggc cggaacacga tcgacgagtt ggccgtactt ggcgcgggcg gccgcttgcc ggacggtcag tgattcgctg ggccactgcg ggtcttcggg ctcgtagttc ttcagcagca tccgcgccag gttcaccttg tgagcttcgg tggggccgtc tgccagcccc agcgcgacac cgccgagtag cacgctgacc agcggtagct gatcggtggt gcccagcgcg ccgtgcacct ggatagcgcg cagaccaatc gatttgagca cttgggatgc caggatcttg caggcgccga tctcggcgcg cgccgcgtgc tcgccggcgg tgtcgatcat ccaggccgcg tgcagcaccg ccagccggaa cggcatcagc tcggtatagg agtcggcgat gaacgcttgc accaactgct tatcggccag cgaactgccc tgggtgaatc gacttttcgc tcggcgtgac atcatctcga ccgcacgctg cgccaccccg atggagcgca tcgcgtgatg cagccggcca ccggccaggc gggtttgcag gatcagaaat ccctgacccg gttccccaag cagcgcgtcc gcaccgactc gaacgtcgtc gtagtgcacc agggagtgac ccggttcgtg tggcagggcg ccgaccaggt ggtggttggc ttcgatggtc aagcccttga ttccagccgg gaccaggaac gtcgacgctc cgtggtgaac ggccacatcc ggattggtga tcgcgacgac gatgaaaaac gatgcgaccg aggcgttgga cgagaaatac ttgcggccgc tgatcaccca atcatcaccg tcgcggacgg cgcgggtggt gaagacgcgc ggatcggcac cgccctgcgg ctcggtcatg gagaagcagg agaagatctc gccggacagc aagccctgca ggtagcgctc cttttgatcc tgggtgccaa agcgcgcaat gatctccgcg ttgccggtgt cgggtgcctg ggtgccgaac acgatcggag cccaggggct gcgtcccagg atctcgttga tcagggtcag tttcaccgcg ccaaagccct gcccgccgag ttccggaccc aaatgtgggg cccacaagcc ttcgtcgcga actcgttgtt ngagnggatc cacgatgcgg cggcgttcat cgttgagcgg caggaactcg cagccgggga acaccacctc gagcggctcc acctcctcgc gtacgaactc gcggatccag tcgagcttct tctcgaactc cggttcggtg gagaagtccc aaggcatcga accaggaccc tgggtctgag cttccagggt acccat

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMPWDFSTEP EFEKKLDWIR EFVREEVEPL EVVFPGCEFL PLNDERRRIV DPLKQRVRDE GLWAPHLGPE LGGQGFGAVK LTLINEILGR SPWAPIVFGT QAPDTGNAEI IARFGTQDQK ERYLQGLLSG EIFSCFSMTE PQGGADPRVF TTRAVRDGDD WVISGRKYFS SNASVASFFI VVAITNPDVA VHHGASTFLV PAGIKGLTIE ANHHLVGALP HEPGHSLVHY DDVRVGADAL LGEPGQGFLI LQTRLAGGRL HHAMRSIGVA QRAVEMMSRR AKSRFTQGSS LADKQLVQAF IADSYTELMP FRLAVLHAAW MIDTAGEHAA RAEIGACKIL ASQVLKSIGL RAIQVHGALG TTDQLPLVSV LLGGVALGLA DGPTEAHKVN LARMLLKNYE PEDPQWPSES LTVRQAAARA KYGQLVDRVP ALPDS

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gccttgggac ttctccaccg aaccggagtt cgagaagaag ctcgactgga tccgcgagtt cgtacgcgag gaggtggagc cgctcgaggt ggtgttcccc ggctgcgagt tcctgccgct caacgatgaa cgccgccgca tcgtggatcc actcaaacaa cgagttcgcg acgaaggctt gtgggcccca catttgggtc cggaactcgg cgggcagggc tttggcgcgg tgaaactgac cctgatcaac gagatcctgg gacgcagccc ctgggctccg atcgtgttcg gcacccaggc acccgacacc ggcaacgcgg agatcattgc gcgctttggc acccaggatc aaaaggagcg ctacctgcag ggcttgctgt ccggcgagat cttctcctgc ttctccatga ccgagccgca gggcggtgcc gatccgcgcg tcttcaccac ccgcgccgtc cgcgacggtg atgattgggt gatcagcggc cgcaagtatt tctcgtccaa cgcctcggtc gcatcgtttt tcatcgtcgt cgcgatcacc aatccggatg tggccgttca ccacggagcg tcgacgttcc tggtcccggc tggaatcaag ggcttgacca tcgaagccaa ccaccacctg gtcggcgccc tgccacacga accgggtcac tccctggtgc actacgacga cgttcgagtc ggtgcggacg cgctgcttgg ggaaccgggt cagggatttc tgatcctgca aacccgcctg gccggtggcc ggctgcatca cgcgatgcgc tccatcgggg tggcgcagcg tgcggtcgag atgatgtcac gccgagcgaa aagtcgattc acccagggca gttcgctggc cgataagcag ttggtgcaag cgttcatcgc cgactcctat accgagctga tgccgttccg gctggcggtg ctgcacgcgg cctggatgat cgacaccgcc ggcgagcacg cggcgcgcgc cgagatcggc gcctgcaaga tcctggcatc ccaagtgctc aaatcgattg gtctgcgcgc tatccaggtg cacggcgcgc tgggcaccac cgatcagcta ccgctggtca gcgtgctact cggcggtgtc gcgctggggc tggcagacgg ccccaccgaa gctcacaagg tgaacctggc gcggatgctg ctgaagaact acgagcccga agacccgcag tggcccagcg aatcactgac cgtccggcaa gcggccgccc gcgccaagta cggccaactc gtcgatcgtg ttccggccct tcccgattca aaacagcacg aacaagttct gcagccaagc ttctcgagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aa

Details for MymaA.00309.b.A1.PS00904

PURIFICATION DATe:	12/9/2010
CONCENTRATION:	63.47mg/ml
OBSERVED MW:	data unavailable
EXPRESSION LEVEL:	Moderate Expression
PROTEIN PURIFICATION BUFFER:	20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	3
VIAL VOLUME:	200µl
PERCENT IDENTITY:	99
PERCENT COVERAGE:	97

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GCCTTGGGAC TTCTCCACCG AACCGGAGTT CGAGAAGAAG CTCGACTGGA TCCGCGAGTT CGTACGCGAG GAGGTGGAGC CGCTCGAGGT GGTGTTCCCC GGCTGCGAGT TCCTGCCGCT CAACGATGAA CGCCGCCGCA TCGTGGATCC ACTCAAACAA CGAGTTCGCG ACGAAGGCTT GTGGGCCCCA CATTTGGGTC CGGAACTCGG CGGGCAGGGC TTTGGCGCGG TGAAACTGAC CCTGATCAAC GAGATCCTGG GACGCAGCCC CTGGGCTCCG ATCGTGTTCG GCACCCAGGC ACCCGACACC GGCAACGCGG AGATCATTGC GCGCTTTGGC ACCCAGGATC AAAAGGAGCG CTACCTGCAG GGCTTGCTGT CCGGCGAGAT CTTCTCCTGC TTCTCCATGA CCGAGCCGCA GGGCGGTGCC GATCCGCGCG TCTTCACCAC CCGCGCCGTC CGCGACGGTG ATGATTGGGT GATCAGCGGC CGCAAGTATT TCTCGTCCAA CGCCTCGGTC GCATCGTTTT TCATCGTCGT CGCGATCACC AATCCGGATG TGGCCGTTCA CCACGGAGCG TCGACGTTCC TGGTCCCGGC TGGAATCAAG GGCTTGACCA TCGAAGCCAA CCACCACCTG GTCGGCGCCC TGCCACACGA ACCGGGTCAC TCCCTGGTGC ACTACGACGA CGTTCGAGTC GGTGCGGACG CGCTGCTTGG GGAACCGGGT CAGGGATTTC TGATCCTGCA AACCCGCCTG GCCGGTGGCC GGCTGCATCA CGCGATGCGC TCCATCGGGG TGGCGCAGCG TGCGGTCGAG ATGATGTCAC GCCGAGCGAA AAGTCGATTC ACCCAGGGCA GTTCGCTGGC CGATAAGCAG TTGGTGCAAG CGTTCATCGC CGACTCCTAT ACCGAGCTGA TGCCGTTCCG GCTGGCGGTG CTGCACGCGG CCTGGATGAT CGACACCGCC GGCGAGCACG CGGCGCGCGC CGAGATCGGC GCCTGCAAGA TCCTGGCATC CCAAGTGCTC AAATCGATTG GTCTGCGCGC TATCCAGGTG CACGGCGCGC TGGGCACCAC CGATCAGCTA CCGCTGGTCA GCGTGCTACT CGGCGGTGTC GCGCTGGGGC TGGCAGACGG CCCCACCGAA GCTCACAAGG TGAACCTGGC GCGGATGCTG CTGAAGAACT ACGAGCCCGA AGACCCGCAG TGGCCCAGCG AATCACTGAC CGTCCGGCAA GCGGCCGCCC GCGCCAAGTA CGGCCAACTC GTCGATCGTG TTCCGGCCCT TCCCGATTCA AAACAGCACG AACAAGTTCT GCAGCCAAGC TTCTCGAGGA TCCGGCTGCT AACAAAGCCC GAAAGGAAGC TGAGTTGGCT GCTGCCACCG CTGAGCAATA ACTAGCATAA CCCCTTGGGG CCTCTAAACG GGTCTTGAGG GGTTTTTTGC TGAAAGGAGG AACTATATCC GGATATCCAC AGGACGGGTG TGGTCGCCAT GATCGCGTAG TCGATAGTGG CTCCAAGTAG CGAAGCGAGC AGGACTGGGC GGCGGCCAAA GCGGTCGGAC AGTGCTCCGA GAACGGGTGC GCATAGAAAT TGCATCAACG CATATAGCGC TAGCAGCACG CCATAGTGAC TGGCGATGCT GTCGGAATGG ACGATATCCC GCAAGAGGCC CGGCAGTACC GGCATAACCA AGCCTATGCC TACAGCATCC AGGGTGACGG TGCCGAGGAT GACGATGAGC GCATTGTTAG ATTTCATACA CGGTGCCTGA CTGCGTTAGC AATTTAACTG TGATAAACTA CCGCATTAAA GCTTATCGAT GATAAGCTGT CAAACATGAG AA