SSGCID: Target Page

MymaA.00305.b

Enoyl-CoA hydratase, EchA12_2

CENTER ID:	MymaA.00305.b
ORGANISM:	Mycobacterium marinum ATCC BAA-535 / M
ASSOCIATED DISEASE:	Cutaneous ulcerations, deep abscesses
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	False
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MymaA.00305.b.A1.GE29988	Full length( MymaA.00305.b )		1	268

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|216594.6.peg.4908
RefSeq:	YP_001852829.1
UniProt:	B2HEE9

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MAAVELQTLQ HNIACVTLNR PTRLNAIDGS LLDAMDDALN ALSGGEFRAA ILTGAGRGFC AGADLSGTGQ PWTKATPTTP PFKATYDAQV RLADQLTRLY ELPIPVIAAV NGVAVGGGLA FALHCDIRIA AEDARFGSVF IKAGFSSMDM GTSYLLPRIV GAGVARELML TGRIIDAVEA YRIRLVHEVV AADELLTAAQ KLAGSIAENN AYGVWQTKIG LNAALDAPSL RHAKEIENRT QIISGFTNNP AEAARAHQEK RPPAWDSL

NT Sequence

ATGGCTGCAG TGGAATTGCA AACTCTTCAG CACAACATTG CTTGCGTCAC CTTGAACCGG CCGACCCGGT TGAACGCCAT CGACGGATCG CTGCTCGACG CGATGGACGA CGCCCTCAAC GCGCTGAGCG GGGGCGAGTT TCGGGCCGCG ATCCTGACCG GCGCCGGGCG CGGGTTTTGT GCCGGAGCGG ATTTGAGCGG CACCGGACAG CCTTGGACGA AGGCCACACC CACCACCCCG CCGTTCAAGG CCACCTACGA TGCCCAGGTC AGACTGGCCG ACCAATTGAC CCGGCTCTAC GAGCTGCCCA TTCCCGTGAT CGCGGCGGTC AACGGCGTCG CGGTCGGTGG TGGGCTGGCC TTTGCGCTGC ATTGCGATAT CCGGATCGCT GCCGAGGACG CCAGGTTCGG ATCGGTGTTC ATCAAGGCCG GGTTCTCGTC GATGGACATG GGCACCAGTT ATCTGCTGCC CAGAATCGTC GGTGCCGGGG TGGCGCGCGA ACTCATGCTG ACCGGCCGCA TCATCGACGC TGTGGAGGCC TATCGGATCA GGCTGGTGCA CGAGGTGGTG GCAGCGGACG AATTGTTGAC TGCGGCGCAG AAGCTGGCCG GGTCGATCGC CGAGAACAAT GCGTATGGCG TGTGGCAGAC CAAGATTGGG CTCAACGCGG CGTTGGATGC CCCCAGCCTG CGGCATGCCA AGGAAATCGA GAACCGTACC CAGATCATCA GCGGCTTCAC CAACAACCCG GCCGAGGCCG CCCGCGCGCA TCAGGAGAAG CGTCCGCCGG CATGGGATTC GCTG

Details for MymaA.00305.b.A1.GE29988

HARVESTED ON:	7/13/2010
SEQUENCED ON:	8/20/2010
EXPECTED MW:	30kDa
OBSERVED MW:	30kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	100
PERCENT COVERAGE:	99

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SMAAVELQTL QHNIACVTLN RPTRLNAIDG SLLDAMDDAL NALSGGEFRA AILTGAGRGF CAGADLSGTG QPWTKATPTT PPFKATYDAQ VRLADQLTRL YELPIPVIAA VNGVAVGGGL AFALHCDIRI AAEDARFGSV FIKAGFSSMD MGTSYLLPRI VGAGVARELM LTGRIIDAVE AYRIRLVHEV VAADELLTAA QKLAGSIAEN NAYGVWQTKI GLNAALDAPS LRHAKEIENR TQIISGFTNN PAEAARAHQE KRPPAW

Validated NT Sequence

cccatgccgg cggacgcttc tcctgatgcg cgcgggcggc ctcggccggg ttgttggtga agccgctgat gatctgggta cggttctcga tttccttggc atgccgcagg ctgggggcat ccaacgccgc gttgagccca atcttggtct gccacacgcc atacgcattg ttctcggcga tcgacccggc cagcttctgc gccgcagtca acaattcgtc cgctgccacc acctcgtgca ccagcctgat ccgataggcc tccacagcgt cgatgatgcg gccggtcagc atgagttcgc gcgccacccc ggcaccgacg attctgggca gcagataact ggtgcccatg tccatcgacg agaacccggc cttgatgaac accgatccga acctggcgtc ctcggcagcg atccggatat cgcaatgcag cgcaaaggcc agcccaccac cgaccgcgac gccgttgacc gccgcgatca cgggaatggg cagctcgtag agccgggtca attggtcggc cagtctgacc tgggcatcgt aggtggcctt gaacggcggg gtggtgggtg tggccttcgt ccaaggctgt ccggtgccgc tcaaatccgc tccggcacaa aacccgcgcc cggcgccggt caggatcgcg gcccgaaact cgcccccgct cagcgcgttg agggcgtcgt ccatcgcgtc gagcagcgat ccgtcgatgg cgttcaaccg ggtcggccgg ttcaaggtga cgcaagcaat gttgtgctga agagtttgca attccactgc agccatcgaa ccaggaccct gggtctgagc ttccagggta cccatatgat ggtgatggtg atgagccat

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggctgcagtg gaattgcaaa ctcttcagca caacattgct tgcgtcacct tgaaccggcc gacccggttg aacgccatcg acggatcgct gctcgacgcg atggacgacg ccctcaacgc gctgagcggg ggcgagtttc gggccgcgat cctgaccggc gccgggcgcg ggttttgtgc cggagcggat ttgagcggca ccggacagcc ttggacgaag gccacaccca ccaccccgcc gttcaaggcc acctacgatg cccaggtcag actggccgac caattgaccc ggctctacga gctgcccatt cccgtgatcg cggcggtcaa cggcgtcgcg gtcggtggtg ggctggcctt tgcgctgcat tgcgatatcc ggatcgctgc cgaggacgcc aggttcggat cggtgttcat caaggccggg ttctcgtcga tggacatggg caccagttat ctgctgccca gaatcgtcgg tgccggggtg gcgcgcgaac tcatgctgac cggccgcatc atcgacgctg tggaggccta tcggatcagg ctggtgcacg aggtggtggc agcggacgaa ttgttgactg cggcgcagaa gctggccggg tcgatcgccg agaacaatgc gtatggcgtg tggcagacca agattgggct caacgcggcg ttggatgccc ccagcctgcg gcatgccaag gaaatcgaga accgtaccca gatcatcagc ggcttcacca acaacccggc cgaggccgcc cgcgcgcatc aggagaagcg tccgccggca tgggattcga aacagcacga acaagttctg cagccaagct tctcgaggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga a