MymaA.00277.b

Lipase/esterase LipN

CENTER ID: MymaA.00277.b
ORGANISM: Mycobacterium marinum ATCC BAA-535 / M
ASSOCIATED DISEASE: Cutaneous ulcerations, deep abscesses
CURRENT STATUS: expressed
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MymaA.00277.b.A1.GE29982 Full length( MymaA.00277.b ) 1 372
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|216594.6.peg.1866
RefSeq: YP_001850050.1
UniProt: B2HIK1

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTNSLPGETD LHPETVHATM SWLSRVQNTV TVVGAKVIPW VPDVAKRLIT RGRSVIIDGN TLDPALQLML SGMRVVGLDG LVIDDDLAAS RAHMREAMLG FPGPQIHVDV EELTLPGPAG DISARHYRPP GDAAAPLLVF YHGGGWALGD LDTHDAVCRL TCRDAGIHVL AIDYRLSPED RAPAAIDDAF AAFEWAHAHA AELGALPGRV AVGGDSAGGN LAAVVSQLAR DSGGPAPIFQ WLIYPRTDFA GRTRSASLFA RGFLLTKRDI DWFHSQYLKG SGIEPTDPRV SPLRAESLAG LAPALIAVAG FDPLRDEGEN YATALRAAGT PVDLRAMGSL THGFLNLFPL GGGCAAATSE LISALRAHLT RV
NT Sequence
ATGACAAATA GCCTGCCAGG TGAGACGGAC CTCCATCCCG AAACGGTGCA CGCGACGATG AGCTGGCTGA GTCGCGTGCA GAACACCGTC ACCGTCGTCG GCGCCAAGGT CATCCCATGG GTTCCCGATG TCGCCAAACG GCTGATCACG CGGGGTCGCT CGGTCATCAT CGACGGAAAC ACCCTCGATC CCGCATTGCA GCTGATGTTG TCGGGCATGC GGGTCGTGGG TCTGGACGGG CTGGTGATCG ATGACGACCT GGCCGCTTCC CGCGCCCACA TGCGCGAGGC GATGTTGGGA TTTCCCGGTC CGCAGATCCA TGTCGATGTC GAGGAATTAA CCCTGCCCGG ACCTGCCGGC GACATCAGCG CCAGGCATTA CCGTCCGCCC GGCGATGCGG CCGCGCCGTT GCTGGTCTTC TACCACGGGG GCGGGTGGGC GCTCGGCGAT CTCGACACCC ACGACGCGGT CTGCCGACTG ACTTGTCGCG ATGCGGGCAT CCATGTCCTG GCGATCGACT ACCGGCTGTC GCCCGAGGAT CGCGCCCCCG CCGCGATCGA CGATGCGTTC GCGGCCTTTG AGTGGGCGCA CGCGCACGCC GCCGAGCTTG GTGCTCTGCC GGGACGGGTT GCGGTCGGCG GCGACAGTGC GGGGGGCAAC CTGGCGGCCG TGGTGAGCCA GCTGGCCCGA GACAGCGGAG GCCCGGCCCC GATTTTCCAG TGGCTCATCT ACCCACGAAC CGACTTTGCC GGGCGGACCC GTTCCGCGAG CCTGTTCGCC CGAGGGTTCT TGCTGACCAA ACGCGACATC GACTGGTTTC ACTCCCAGTA CCTGAAGGGC TCCGGCATCG AGCCCACGGA CCCGCGGGTA TCGCCGTTGC GAGCCGAATC ACTGGCCGGG CTGGCGCCGG CGCTGATCGC GGTGGCGGGC TTCGACCCGT TGCGTGACGA AGGCGAGAAC TACGCGACCG CCCTGCGGGC CGCCGGCACC CCGGTTGACC TGCGCGCCAT GGGGTCGCTG ACGCACGGGT TCCTCAATCT GTTTCCGCTC GGTGGTGGTT GTGCCGCCGC AACCAGCGAG CTGATTTCTG CGCTGCGTGC ACACCTGACC CGGGTT
Details for MymaA.00277.b.A1.GE29982
HARVESTED ON: 7/13/2010
SEQUENCED ON: 12/5/2014
EXPECTED MW: 41kDa
OBSERVED MW: 41kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 96
PERCENT COVERAGE: 78
Validated AA Sequence
MGTLEAQTQG PGSMTNSLPG ETDLHPETVH ATMSWLSRVQ NTVTVVGAKV IPWVPDVAKR LITRGRSVII DGNTLDPALQ LMLSGMRVVG LDGLVIDDDL AASRAHMREA MLGFPGPQIH VDVEELTLPG PAGDISARHY RPPGDAAAPL LVFYHGGGWA LGDLDTHDAV CRLTCRDAGI HVLAIDYRLS PEDRAPAAID DAFAAFEWAH AHAAELGALP GRVAVGGDSA GGNLAAVVSQ LXRDSGXPAP IFQWLIYPRT DFAGRTRSAS LFAXXXLLTK RDIDWFHSXY LKGSXIXPXD PXVSXX
Validated NT Sequence
ggctcatcac catcaccatc atatgggtac cctggaagct cagacccagg gtcctggttc gatgacaaat agcctgccag gtgagacgga cctccatccc gaaacggtgc acgcgacgat gagctggctg agtcgcgtgc agaacaccgt caccgtcgtc ggcgccaagg tcatcccatg ggttcccgat gtcgccaaac ggctgatcac gcggggtcgc tcggtcatca tcgacggaaa caccctcgat cccgcattgc agctgatgtt gtcgggcatg cgggtcgtgg gtctggacgg gctggtgatc gatgacgacc tggccgcttc ccgcgcccac atgcgcgagg cgatgttggg atttcccggt ccgcagatcc atgtcgatgt cgaggaatta accctgcccg gacctgccgg cgacatcagc gccaggcatt accgtccgcc cggcgatgcg gccgcgccgt tgctggtctt ctaccacggg ggcgggtggg cgctcggcga tctcgacacc cacgacgcgg tctgccgact gacttgtcgc gatgcgggca tccatgtcct ggcgatcgac taccggctgt cgcccgagga tcgcgccccc gccgcgatcg acgatgcgtt cgcggccttt gagtgggcgc acgcgcacgc cgccgagctt ggtgctctgc cgggacgggt tgcggtcggc ggcgacagtg cggggggcaa cctggcggcc gtggtgagcc agctggnccg agacagcggn nncccggccc cgattttcca gtggctcatc tacccacgaa ccgactttgc cgggcggacc cgttccgcga gcctgttcgc ccnangnntc ttgctgacca aacgcgacat cgactggttt cactcccngt acctgaaggg ctccgncatc nancccanng acccgcnggt atcnnngntg c
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMTNSLPGET DLHPETVHAT MSWLSRVQNT VTVVGAKVIP WVPDVAKRLI TRGRSVIIDG NTLDPALQLM LSGMRVVGLD GLVIDDDLAA SRAHMREAML GFPGPQIHVD VEELTLPGPA GDISARHYRP PGDAAAPLLV FYHGGGWALG DLDTHDAVCR LTCRDAGIHV LAIDYRLSPE DRAPAAIDDA FAAFEWAHAH AAELGALPGR VAVGGDSAGG NLAAVVSQLA RDSGGPAPIF QWLIYPRTDF AGRTRSASLF ARGFLLTKRD IDWFHSQYLK GSGIEPTDPR VSPLRAESLA GLAPALIAVA GFDPLRDEGE NYATALRAAG TPVDLRAMGS LTHGFLNLFP LGGGCAAATS ELISALRAHL TR
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gacaaatagc ctgccaggtg agacggacct ccatcccgaa acggtgcacg cgacgatgag ctggctgagt cgcgtgcaga acaccgtcac cgtcgtcggc gccaaggtca tcccatgggt tcccgatgtc gccaaacggc tgatcacgcg gggtcgctcg gtcatcatcg acggaaacac cctcgatccc gcattgcagc tgatgttgtc gggcatgcgg gtcgtgggtc tggacgggct ggtgatcgat gacgacctgg ccgcttcccg cgcccacatg cgcgaggcga tgttgggatt tcccggtccg cagatccatg tcgatgtcga ggaattaacc ctgcccggac ctgccggcga catcagcgcc aggcattacc gtccgcccgg cgatgcggcc gcgccgttgc tggtcttcta ccacgggggc gggtgggcgc tcggcgatct cgacacccac gacgcggtct gccgactgac ttgtcgcgat gcgggcatcc atgtcctggc gatcgactac cggctgtcgc ccgaggatcg cgcccccgcc gcgatcgacg atgcgttcgc ggcctttgag tgggcgcacg cgcacgccgc cgagcttggt gctctgccgg gacgggttgc ggtcggcggc gacagtgcgg ggggcaacct ggcggccgtg gtgagccagc tggcccgaga cagcggaggc ccggccccga ttttccagtg gctcatctac ccacgaaccg actttgccgg gcggacccgt tccgcgagcc tgttcgcccg agggttcttg ctgaccaaac gcgacatcga ctggtttcac tcccagtacc tgaagggctc cggcatcgag cccacggacc cgcgggtatc gccgttgcga gccgaatcac tggccgggct ggcgccggcg ctgatcgcgg tggcgggctt cgacccgttg cgtgacgaag gcgagaacta cgcgaccgcc ctgcgggccg ccggcacccc ggttgacctg cgcgccatgg ggtcgctgac gcacgggttc ctcaatctgt ttccgctcgg tggtggttgt gccgccgcaa ccagcgagct gatttctgcg ctgcgtgcac acctgacccg gaaacagcac gaacaagttc tgcagccaag cttctcgagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaa