MymaA.00272.a

Putative 8-amino-7-oxononanoate synthase/2-amino-3-ketobutyrate coenzyme A ligase

CENTER ID: MymaA.00272.a
ORGANISM: Mycobacterium marinum ATCC BAA-535 / M
ASSOCIATED DISEASE: Cutaneous ulcerations, deep abscesses
CURRENT STATUS: purified
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MymaA.00272.a.A1.GE29980 Full length( MymaA.00272.a ) 1 389
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MymaA.00272.a.A1.PS01012 Full length( MymaA.00272.a ) 1 389

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|216594.6.peg.2540
RefSeq: YP_001850688.1
UniProt: B2HQ90

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MGAPTQIPIE TSPLAWLDAV ERQRRDAGLR RSLRPRPPVG TELDLASNDY LGLSQHPDVI EGGVQALRIW GAGATGSRLV TGDTELHQQL EAELAEYVGA AAGLLFSSGY TANLGAVVGL SGPGSLLVSD AYSHASLVDA CRLSRARVVV TPHRDVAAVD AALASRDEQR AMVITDSVFS ADGTLAPLRE LLAACRRHRA LLVIDEAHGL GVRGGGRGLL HELGLAGAPD VVLTTTLSKA LGSQGGAVLG PAAVRAHLID AARPFIFDTG LAPAAVGAAR AALGVLKAEQ WRPDAVLQNA RELADICDVP ETPQSAVVSV LLGDPEVALA AAAACLDAGV KVGCFRPPTV PAGTSRLRLT ARASLSPDEM ELARRVLTDV LLGPAAARR
NT Sequence
ATGGGCGCCC CCACACAGAT TCCGATTGAG ACGTCGCCAC TGGCCTGGCT GGACGCTGTC GAACGGCAGC GCCGCGATGC TGGGCTGCGT CGCTCGCTGC GCCCGCGTCC TCCCGTCGGT ACCGAGTTGG ACCTTGCGTC CAACGATTAC CTGGGGCTGT CCCAGCACCC CGACGTGATT GAAGGCGGTG TCCAGGCACT GCGCATCTGG GGCGCAGGCG CCACCGGTTC GCGCCTGGTC ACCGGCGACA CCGAGCTGCA CCAGCAGTTG GAAGCCGAAC TCGCCGAATA CGTCGGCGCT GCCGCGGGCC TGTTGTTTTC GTCGGGCTAC ACGGCGAACT TGGGAGCCGT CGTCGGTCTG TCCGGGCCGG GTTCGCTTTT GGTCTCCGAT GCGTACTCGC ACGCGTCCCT GGTGGACGCC TGCCGCCTTT CGCGCGCTCG GGTGGTCGTG ACGCCGCACC GCGACGTTGC TGCGGTGGAC GCGGCGCTGG CCTCGCGCGA CGAGCAACGG GCCATGGTCA TCACCGACTC GGTGTTCAGC GCCGACGGCA CCTTGGCCCC GCTTCGTGAG TTGCTCGCCG CCTGCCGCCG GCACCGGGCC CTGCTCGTCA TCGACGAGGC GCACGGACTG GGCGTTCGCG GTGGCGGGCG CGGGCTGTTG CACGAGCTGG GGCTGGCGGG TGCGCCCGAT GTGGTGCTGA CCACGACGCT GTCGAAAGCT CTCGGCAGCC AGGGCGGTGC CGTTCTGGGG CCGGCCGCGG TGCGTGCGCA CCTCATCGAT GCGGCCCGGC CGTTCATCTT CGACACCGGT TTGGCGCCGG CCGCAGTGGG CGCGGCGCGG GCCGCGCTGG GTGTGCTCAA GGCCGAGCAG TGGCGGCCCG ACGCGGTGTT GCAGAACGCC CGTGAACTCG CCGACATCTG CGACGTGCCC GAGACGCCGC AGTCCGCGGT GGTGTCCGTG TTGCTGGGCG ACCCGGAGGT GGCGCTGGCC GCCGCGGCGG CCTGTCTGGA CGCCGGTGTC AAGGTGGGGT GCTTCCGGCC CCCGACGGTG CCCGCCGGGA CATCACGGCT GAGACTGACG GCGCGCGCGT CGTTGAGTCC TGACGAGATG GAGCTGGCCC GGCGGGTGCT GACCGACGTT CTTCTCGGCC CTGCCGCGGC ACGCCGT
Details for MymaA.00272.a.A1.GE29980
HARVESTED ON: 7/13/2010
SEQUENCED ON: 8/18/2010
EXPECTED MW: 42kDa
OBSERVED MW: 42kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 98
PERCENT COVERAGE: 98
Validated AA Sequence
MGTLEAQTQG PGSMGAPTQI PIETSPLAWL DAVERQRRDA GLRRSLRPRP PVGTELDLAS NDYLGLSXHP DVIEGGVQAL RIWGAGATGS RLVTGDTELH QQLEAELAXY VGAAAGLLFS SGYTANLGAV VGLSGPGSLL XSDAYSHASX VDACXLSRAR VVVTPHRDVA AVDAALASRD EQRAMVITDS VFSADGTLAP LRELLAACRR HRALLVIDEA HGLGVRGGGR GLLHELGLAG APDVVLTTTL SKALGSQGGA VLGPAAVRAH LIDAARPFIF DTGLAPAAVG AARAALGVLK AEQWRPDAVL QNARELADIC DVPETPQSAV VSVLLGDPEV ALAAAAACLD AGVKVGCFRP PTVPAGTSRL RLTARASLSP DEMELARRVL TDVLLGPAAA R
Validated NT Sequence
ttattagcgt gccgcggcag ggccgagaag aacgtcggtc agcacccgcc gggccagctc catctcgtca ggactcaacg acgcgcgcgc cgtcagtctc agccgtgatg tcccggcggg caccgtcggg ggccggaagc accccacctt gacaccggcg tccagacagg ccgccgcggc ggccagcgcc acctccgggt cgcccagcaa cacggacacc accgcggact gcggcgtctc gggcacgtcg cagatgtcgg cgagttcacg ggcgttctgc aacaccgcgt cgggccgcca ctgctcggcc ttgagcacac ccagcgcggc ccgcgccgcg cccactgcgg ccggcgccaa accggtgtcg aagatgaacg gccgggccgc atcgatgagg tgcgcacgca ccgcggccgg ccccagaacg gcaccgccct ggctgccgag agctttcgac agcgtcgtgg tcagcaccac atcgggcgca cccgccagcc ccagctcgtg caacagcccg cgcccgccac cgcgaacgcc cagtccgtgc gcctcgtcga tgacgagcag ggcccggtgc cggcggcagg cggcgagcaa ctcacgaagc ggggccaagg tgccgtcggc gctgaacacc gagtcggtga tgaccatggc ccgttgctcg tcgcgcgagg ccagcgccgc gtccaccgca gcaacgtcgc ggtgcggcgt cacgaccacc cgagcgcgcg aaaggnggca ngcgtccacc anggacgcgt gcgagtacgc atcggananc aaaagcgaac ccggcccgga cagaccgacg acggctccca agttcgccgt gtagcccgac gaaaacaaca ggcccgcggc agcgccgacg tattnggcga gttcggcttc caactgctgg tgcagctcgg tgtcgccggt gaccaggcgc gaaccggtgg cgcctgcgcc ccagatgcgc agtgcctgga caccgccttc aatcacgtcg gggtgntggg acagccccag gtaatcgttg gacgcaaggt ccaactcggt accgacggga ggacgcgggc gcagcgagcg acgcagccca gcatcgcggc gctgccgttc gacagcgtcc agccaggcca gtggcgacgt ctcaatcgga atctgtgtgg gggcgcccat cgaaccagga ccctgggtct gagcttccag ggtacccata
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMGAPTQIPI ETSPLAWLDA VERQRRDAGL RRSLRPRPPV GTELDLASND YLGLSQHPDV IEGGVQALRI WGAGATGSRL VTGDTELHQQ LEAELAEYVG AAAGLLFSSG YTANLGAVVG LSGPGSLLVS DAYSHASLVD ACRLSRARVV VTPHRDVAAV DAALASRDEQ RAMVITDSVF SADGTLAPLR ELLAACRRHR ALLVIDEAHG LGVRGGGRGL LHELGLAGAP DVVLTTTLSK ALGSQGGAVL GPAAVRAHLI DAARPFIFDT GLAPAAVGAA RAALGVLKAE QWRPDAVLQN ARELADICDV PETPQSAVVS VLLGDPEVAL AAAAACLDAG VKVGCFRPPT VPAGTSRLRL TARASLSPDE MELARRVLTD VLLGPAAAR
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gggcgccccc acacagattc cgattgagac gtcgccactg gcctggctgg acgctgtcga acggcagcgc cgcgatgctg ggctgcgtcg ctcgctgcgc ccgcgtcctc ccgtcggtac cgagttggac cttgcgtcca acgattacct ggggctgtcc cagcaccccg acgtgattga aggcggtgtc caggcactgc gcatctgggg cgcaggcgcc accggttcgc gcctggtcac cggcgacacc gagctgcacc agcagttgga agccgaactc gccgaatacg tcggcgctgc cgcgggcctg ttgttttcgt cgggctacac ggcgaacttg ggagccgtcg tcggtctgtc cgggccgggt tcgcttttgg tctccgatgc gtactcgcac gcgtccctgg tggacgcctg ccgcctttcg cgcgctcggg tggtcgtgac gccgcaccgc gacgttgctg cggtggacgc ggcgctggcc tcgcgcgacg agcaacgggc catggtcatc accgactcgg tgttcagcgc cgacggcacc ttggccccgc ttcgtgagtt gctcgccgcc tgccgccggc accgggccct gctcgtcatc gacgaggcgc acggactggg cgttcgcggt ggcgggcgcg ggctgttgca cgagctgggg ctggcgggtg cgcccgatgt ggtgctgacc acgacgctgt cgaaagctct cggcagccag ggcggtgccg ttctggggcc ggccgcggtg cgtgcgcacc tcatcgatgc ggcccggccg ttcatcttcg acaccggttt ggcgccggcc gcagtgggcg cggcgcgggc cgcgctgggt gtgctcaagg ccgagcagtg gcggcccgac gcggtgttgc agaacgcccg tgaactcgcc gacatctgcg acgtgcccga gacgccgcag tccgcggtgg tgtccgtgtt gctgggcgac ccggaggtgg cgctggccgc cgcggcggcc tgtctggacg ccggtgtcaa ggtggggtgc ttccggcccc cgacggtgcc cgccgggaca tcacggctga gactgacggc gcgcgcgtcg ttgagtcctg acgagatgga gctggcccgg cgggtgctga ccgacgttct tctcggccct gccgcggcac gcaaacagca cgaacaagtt ctgcagccaa gcttctcgag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaa
Details for MymaA.00272.a.A1.PS01012
PURIFICATION DATe: 3/10/2011
CONCENTRATION: 16.03mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Low Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 2
VIAL VOLUME: 100µl
PERCENT IDENTITY: 98
PERCENT COVERAGE: 98
Protocol Notes
notes unavailable
Validated AA Sequence
MGTLEAQTQG PGSMGAPTQI PIETSPLAWL DAVERQRRDA GLRRSLRPRP PVGTELDLAS NDYLGLSXHP DVIEGGVQAL RIWGAGATGS RLVTGDTELH QQLEAELAXY VGAAAGLLFS SGYTANLGAV VGLSGPGSLL XSDAYSHASX VDACXLSRAR VVVTPHRDVA AVDAALASRD EQRAMVITDS VFSADGTLAP LRELLAACRR HRALLVIDEA HGLGVRGGGR GLLHELGLAG APDVVLTTTL SKALGSQGGA VLGPAAVRAH LIDAARPFIF DTGLAPAAVG AARAALGVLK AEQWRPDAVL QNARELADIC DVPETPQSAV VSVLLGDPEV ALAAAAACLD AGVKVGCFRP PTVPAGTSRL RLTARASLSP DEMELARRVL TDVLLGPAAA R
Validated NT Sequence
ttattagcgt gccgcggcag ggccgagaag aacgtcggtc agcacccgcc gggccagctc catctcgtca ggactcaacg acgcgcgcgc cgtcagtctc agccgtgatg tcccggcggg caccgtcggg ggccggaagc accccacctt gacaccggcg tccagacagg ccgccgcggc ggccagcgcc acctccgggt cgcccagcaa cacggacacc accgcggact gcggcgtctc gggcacgtcg cagatgtcgg cgagttcacg ggcgttctgc aacaccgcgt cgggccgcca ctgctcggcc ttgagcacac ccagcgcggc ccgcgccgcg cccactgcgg ccggcgccaa accggtgtcg aagatgaacg gccgggccgc atcgatgagg tgcgcacgca ccgcggccgg ccccagaacg gcaccgccct ggctgccgag agctttcgac agcgtcgtgg tcagcaccac atcgggcgca cccgccagcc ccagctcgtg caacagcccg cgcccgccac cgcgaacgcc cagtccgtgc gcctcgtcga tgacgagcag ggcccggtgc cggcggcagg cggcgagcaa ctcacgaagc ggggccaagg tgccgtcggc gctgaacacc gagtcggtga tgaccatggc ccgttgctcg tcgcgcgagg ccagcgccgc gtccaccgca gcaacgtcgc ggtgcggcgt cacgaccacc cgagcgcgcg aaaggnggca ngcgtccacc anggacgcgt gcgagtacgc atcggananc aaaagcgaac ccggcccgga cagaccgacg acggctccca agttcgccgt gtagcccgac gaaaacaaca ggcccgcggc agcgccgacg tattnggcga gttcggcttc caactgctgg tgcagctcgg tgtcgccggt gaccaggcgc gaaccggtgg cgcctgcgcc ccagatgcgc agtgcctgga caccgccttc aatcacgtcg gggtgntggg acagccccag gtaatcgttg gacgcaaggt ccaactcggt accgacggga ggacgcgggc gcagcgagcg acgcagccca gcatcgcggc gctgccgttc gacagcgtcc agccaggcca gtggcgacgt ctcaatcgga atctgtgtgg gggcgcccat cgaaccagga ccctgggtct gagcttccag ggtacccata
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMGAPTQIPI ETSPLAWLDA VERQRRDAGL RRSLRPRPPV GTELDLASND YLGLSQHPDV IEGGVQALRI WGAGATGSRL VTGDTELHQQ LEAELAEYVG AAAGLLFSSG YTANLGAVVG LSGPGSLLVS DAYSHASLVD ACRLSRARVV VTPHRDVAAV DAALASRDEQ RAMVITDSVF SADGTLAPLR ELLAACRRHR ALLVIDEAHG LGVRGGGRGL LHELGLAGAP DVVLTTTLSK ALGSQGGAVL GPAAVRAHLI DAARPFIFDT GLAPAAVGAA RAALGVLKAE QWRPDAVLQN ARELADICDV PETPQSAVVS VLLGDPEVAL AAAAACLDAG VKVGCFRPPT VPAGTSRLRL TARASLSPDE MELARRVLTD VLLGPAAAR
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGGCGCCCCC ACACAGATTC CGATTGAGAC GTCGCCACTG GCCTGGCTGG ACGCTGTCGA ACGGCAGCGC CGCGATGCTG GGCTGCGTCG CTCGCTGCGC CCGCGTCCTC CCGTCGGTAC CGAGTTGGAC CTTGCGTCCA ACGATTACCT GGGGCTGTCC CAGCACCCCG ACGTGATTGA AGGCGGTGTC CAGGCACTGC GCATCTGGGG CGCAGGCGCC ACCGGTTCGC GCCTGGTCAC CGGCGACACC GAGCTGCACC AGCAGTTGGA AGCCGAACTC GCCGAATACG TCGGCGCTGC CGCGGGCCTG TTGTTTTCGT CGGGCTACAC GGCGAACTTG GGAGCCGTCG TCGGTCTGTC CGGGCCGGGT TCGCTTTTGG TCTCCGATGC GTACTCGCAC GCGTCCCTGG TGGACGCCTG CCGCCTTTCG CGCGCTCGGG TGGTCGTGAC GCCGCACCGC GACGTTGCTG CGGTGGACGC GGCGCTGGCC TCGCGCGACG AGCAACGGGC CATGGTCATC ACCGACTCGG TGTTCAGCGC CGACGGCACC TTGGCCCCGC TTCGTGAGTT GCTCGCCGCC TGCCGCCGGC ACCGGGCCCT GCTCGTCATC GACGAGGCGC ACGGACTGGG CGTTCGCGGT GGCGGGCGCG GGCTGTTGCA CGAGCTGGGG CTGGCGGGTG CGCCCGATGT GGTGCTGACC ACGACGCTGT CGAAAGCTCT CGGCAGCCAG GGCGGTGCCG TTCTGGGGCC GGCCGCGGTG CGTGCGCACC TCATCGATGC GGCCCGGCCG TTCATCTTCG ACACCGGTTT GGCGCCGGCC GCAGTGGGCG CGGCGCGGGC CGCGCTGGGT GTGCTCAAGG CCGAGCAGTG GCGGCCCGAC GCGGTGTTGC AGAACGCCCG TGAACTCGCC GACATCTGCG ACGTGCCCGA GACGCCGCAG TCCGCGGTGG TGTCCGTGTT GCTGGGCGAC CCGGAGGTGG CGCTGGCCGC CGCGGCGGCC TGTCTGGACG CCGGTGTCAA GGTGGGGTGC TTCCGGCCCC CGACGGTGCC CGCCGGGACA TCACGGCTGA GACTGACGGC GCGCGCGTCG TTGAGTCCTG ACGAGATGGA GCTGGCCCGG CGGGTGCTGA CCGACGTTCT TCTCGGCCCT GCCGCGGCAC GCAAACAGCA CGAACAAGTT CTGCAGCCAA GCTTCTCGAG GATCCGGCTG CTAACAAAGC CCGAAAGGAA GCTGAGTTGG CTGCTGCCAC CGCTGAGCAA TAACTAGCAT AACCCCTTGG GGCCTCTAAA CGGGTCTTGA GGGGTTTTTT GCTGAAAGGA GGAACTATAT CCGGATATCC ACAGGACGGG TGTGGTCGCC ATGATCGCGT AGTCGATAGT GGCTCCAAGT AGCGAAGCGA GCAGGACTGG GCGGCGGCCA AAGCGGTCGG ACAGTGCTCC GAGAACGGGT GCGCATAGAA ATTGCATCAA CGCATATAGC GCTAGCAGCA CGCCATAGTG ACTGGCGATG CTGTCGGAAT GGACGATATC CCGCAAGAGG CCCGGCAGTA CCGGCATAAC CAAGCCTATG CCTACAGCAT CCAGGGTGAC GGTGCCGAGG ATGACGATGA GCGCATTGTT AGATTTCATA CACGGTGCCT GACTGCGTTA GCAATTTAAC TGTGATAAAC TACCGCATTA AAGCTTATCG ATGATAAGCT GTCAAACATG AGAA