MymaA.00250.c

Conserved hypothetical oxidase

CENTER ID: MymaA.00250.c
ORGANISM: Mycobacterium marinum ATCC BAA-535 / M
ASSOCIATED DISEASE: Cutaneous ulcerations, deep abscesses
CURRENT STATUS: soluble
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MymaA.00250.c.A1.GE29978 Full length( MymaA.00250.c ) 1 185
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|216594.6.peg.3887
RefSeq: YP_001851905.1
UniProt: B2HL74

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSVDAPNTAA EQGPEILTPD VFREFMSSYP SGVTIVTTYD QHGAPRGFTC SSMCSVTMEP PTLLLCVNTR STTLDAVQDC TRFAVNLLHS GGESAARVFS SVQEDRFSKV RWRASTIGGL PILYEDAHAI AECRLTATRN VGTHTVLFGE IDAIILDHQP LHYRADMNPL LYCRRQFTAF PTQQP
NT Sequence
ATGTCTGTTG ACGCACCAAA CACCGCGGCC GAACAGGGCC CGGAGATCCT TACACCGGAC GTGTTCCGCG AGTTCATGAG CAGCTATCCC AGCGGCGTAA CCATTGTCAC CACCTACGAC CAGCATGGCG CTCCTCGCGG ATTCACCTGC TCCTCGATGT GCAGCGTGAC CATGGAGCCT CCGACCTTGC TGCTGTGCGT GAACACCCGC AGTACCACCC TGGATGCGGT TCAAGACTGC ACGCGCTTCG CCGTGAATCT GTTGCACAGC GGCGGCGAGT CCGCCGCCCG CGTCTTTTCC TCCGTTCAAG AGGACCGCTT CAGCAAGGTT CGCTGGCGAG CGAGCACCAT AGGCGGACTG CCCATTCTGT ACGAAGACGC CCACGCCATC GCTGAATGTC GCCTCACGGC CACCCGCAAC GTGGGAACCC ACACAGTTCT ATTCGGCGAG ATCGACGCCA TCATTCTGGA CCACCAACCG CTTCACTACC GCGCAGACAT GAACCCGCTG CTGTATTGCC GGCGGCAGTT CACCGCGTTC CCGACCCAGC AGCCG
Details for MymaA.00250.c.A1.GE29978
HARVESTED ON: 7/13/2010
SEQUENCED ON: 8/18/2010
EXPECTED MW: 22kDa
OBSERVED MW: 22kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 98
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMSVDAPNTA AEQGPEILTP DVFREFMSSY PSGVTIVTTY DQHGAPRGFT CSSMCSVTME PPTLLLCVNT RSTTLDAVQD CTRFAVNLLH SGGESAARVF SSVQEDRFSK VRWRASTIGG LPILYEDAHA IAECRLTATR NVGTHTVLFG EIDAIILDHQ PLHYRADMNP LLYCRRQFTA FP
Validated NT Sequence
gtcgggaacg cggtgaactg ccgccggcaa tacagcagcg ggttcatgtc tgcgcggtag tgaagcggtt ggtggtccag aatgatggcg tcgatctcgc cgaatagaac tgtgtgggtt cccacgttgc gggtggccgt gaggcgacat tcagcgatgg cgtgggcgtc ttcgtacaga atgggcagtc cgcctatggt gctcgctcgc cagcgaacct tgctgaagcg gtcctcttga acggaggaaa agacgcgggc ggcggactcg ccgccgctgt gcaacagatt cacggcgaag cgcgtgcagt cttgaaccgc atccagggtg gtactgcggg tgttcacgca cagcagcaag gtcggaggct ccatggtcac gctgcacatc gaggagcagg tgaatccgcg aggagcgcca tgctggtcgt aggtggtgac aatggttacg ccgctgggat agctgctcat gaactcgcgg aacacgtccg gtgtaaggat ctccgggccc tgttcggccg cggtgtttgg tgcgtcaaca gacatcgaac caggaccctg ggtctgagct tccagggtac ccatatgatg gtgatggtga tgagccat
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMSVDAPNTA AEQGPEILTP DVFREFMSSY PSGVTIVTTY DQHGAPRGFT CSSMCSVTME PPTLLLCVNT RSTTLDAVQD CTRFAVNLLH SGGESAARVF SSVQEDRFSK VRWRASTIGG LPILYEDAHA IAECRLTATR NVGTHTVLFG EIDAIILDHQ PLHYRADMNP LLYCRRQFTA FPTQQ
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gtctgttgac gcaccaaaca ccgcggccga acagggcccg gagatcctta caccggacgt gttccgcgag ttcatgagca gctatcccag cggcgtaacc attgtcacca cctacgacca gcatggcgct cctcgcggat tcacctgctc ctcgatgtgc agcgtgacca tggagcctcc gaccttgctg ctgtgcgtga acacccgcag taccaccctg gatgcggttc aagactgcac gcgcttcgcc gtgaatctgt tgcacagcgg cggcgagtcc gccgcccgcg tcttttcctc cgttcaagag gaccgcttca gcaaggttcg ctggcgagcg agcaccatag gcggactgcc cattctgtac gaagacgccc acgccatcgc tgaatgtcgc ctcacggcca cccgcaacgt gggaacccac acagttctat tcggcgagat cgacgccatc attctggacc accaaccgct tcactaccgc gcagacatga acccgctgct gtattgccgg cggcagttca ccgcgttccc gacccagcag aaacagcacg aacaagttct gcagccaagc ttctcgagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aa