MymaA.00250.a

Oxidoreductase

CENTER ID: MymaA.00250.a
ORGANISM: Mycobacterium marinum ATCC BAA-535 / M
ASSOCIATED DISEASE: Cutaneous ulcerations, deep abscesses
CURRENT STATUS: crystallized
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

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Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MymaA.00250.a.A1.GE29976 Full length( MymaA.00250.a ) 1 199
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MymaA.00250.a.A1.PW29844 Full length( MymaA.00250.a ) 1 199

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|216594.6.peg.1825
RefSeq: YP_001850009.1
UniProt: B2HIG0

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSQPALCIHD VDVVGEPFED MMAMLDAPVF VVTTQADAVP SGCLVSFATQ TSVQPPSFMI GMPTTSRMVE PASRSDCVAV HVLARRHQAL AELFGSQAGE EADKFERCSW RAGPYGMPIL DDAAAWFVGK ILSRSDVGDH VAYLLEPIAV WAPDAPEELL YLSDLDIEDF EPGQEEPTRF HKPAEVTRRY GVRFTLDYP
NT Sequence
GTGTCACAAC CGGCGTTGTG CATCCATGAT GTTGATGTGG TCGGTGAACC GTTCGAAGAC ATGATGGCGA TGCTGGACGC GCCGGTGTTT GTGGTGACAA CCCAGGCCGA CGCGGTCCCG TCGGGTTGCC TGGTCAGTTT TGCCACCCAG ACCAGCGTGC AGCCACCAAG TTTCATGATC GGGATGCCCA CCACCAGCCG CATGGTGGAG CCGGCGAGCC GCTCCGACTG TGTCGCGGTG CACGTGCTGG CCCGTCGTCA CCAGGCCCTG GCCGAACTCT TCGGCAGCCA AGCGGGCGAG GAGGCTGACA AATTCGAACG TTGCTCGTGG CGCGCCGGTC CCTACGGCAT GCCGATTCTC GACGATGCGG CCGCGTGGTT CGTCGGCAAG ATCTTGAGCC GCAGCGACGT TGGCGACCAC GTGGCCTACC TGCTGGAGCC GATTGCTGTC TGGGCTCCCG ATGCCCCGGA AGAACTGCTC TACCTGTCCG ACCTCGATAT TGAGGACTTC GAACCCGGCC AGGAGGAGCC GACCCGGTTC CACAAACCCG CTGAGGTGAC GCGCCGATAC GGCGTGCGAT TCACCCTCGA CTATCCC
Details for MymaA.00250.a.A1.GE29976
HARVESTED ON: 7/13/2010
SEQUENCED ON: 8/20/2010
EXPECTED MW: 24kDa
OBSERVED MW: 26kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 99
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMVSQPALCI HDVDVVGEPF EDMMAMLDAP VFVVTTQADA VPSGCLVSFA TQTSVQPPSF MIGMPTTSRM VEPASRSDCV AVHVLARRHQ ALAELFGSQA GEEADKFERC SWRAGPYGMP ILDDAAAWFV GKILSRSDVG DHVAYLLEPI AVWAPDAPEE LLYLSDLDIE DFEPGQEEPT RFHKPAEVTR RYGVRFTL
Validated NT Sequence
cgagggtgaa tcgcacgccg tatcggcgcg tcacctcagc gggtttgtgg aaccgggtcg gctcctcctg gccgggttcg aagtcctcaa tatcgaggtc ggacaggtag agcagttctt ccggggcatc gggagcccag acagcaatcg gctccagcag gtaggccacg tggtcgccaa cgtcgctgcg gctcaagatc ttgccgacga accacgcggc cgcatcgtcg agaatcggca tgccgtaggg accggcgcgc cacgagcaac gttcgaattt gtcagcctcc tcgcccgctt ggctgccgaa gagttcggcc agggcctggt gacgacgggc cagcacgtgc accgcgacac agtcggagcg gctcgccggc tccaccatgc ggctggtggt gggcatcccg atcatgaaac ttggtggctg cacgctggtc tgggtggcaa aactgaccag gcaacccgac gggaccgcgt cggcctgggt tgtcaccaca aacaccggcg cgtccagcat cgccatcatg tcttcgaacg gttcaccgac cacatcaaca tcatggatgc acaacgccgg ttgtgacacc atcgaaccag gaccctgggt ctgagcttcc agggtaccca tatgatggtg atggtgatga gccat
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVSQPALCI HDVDVVGEPF EDMMAMLDAP VFVVTTQADA VPSGCLVSFA TQTSVQPPSF MIGMPTTSRM VEPASRSDCV AVHVLARRHQ ALAELFGSQA GEEADKFERC SWRAGPYGMP ILDDAAAWFV GKILSRSDVG DHVAYLLEPI AVWAPDAPEE LLYLSDLDIE DFEPGQEEPT RFHKPAEVTR RYGVRFTLDY
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtgtcacaa ccggcgttgt gcatccatga tgttgatgtg gtcggtgaac cgttcgaaga catgatggcg atgctggacg cgccggtgtt tgtggtgaca acccaggccg acgcggtccc gtcgggttgc ctggtcagtt ttgccaccca gaccagcgtg cagccaccaa gtttcatgat cgggatgccc accaccagcc gcatggtgga gccggcgagc cgctccgact gtgtcgcggt gcacgtgctg gcccgtcgtc accaggccct ggccgaactc ttcggcagcc aagcgggcga ggaggctgac aaattcgaac gttgctcgtg gcgcgccggt ccctacggca tgccgattct cgacgatgcg gccgcgtggt tcgtcggcaa gatcttgagc cgcagcgacg ttggcgacca cgtggcctac ctgctggagc cgattgctgt ctgggctccc gatgccccgg aagaactgct ctacctgtcc gacctcgata ttgaggactt cgaacccggc caggaggagc cgacccggtt ccacaaaccc gctgaggtga cgcgccgata cggcgtgcga ttcaccctcg actataaaca gcacgaacaa gttctgcagc caagcttctc gaggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaa
Details for MymaA.00250.a.A1.PW29844
PURIFICATION DATe: 9/15/2010
CONCENTRATION: 8.02mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Low Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 7
VIAL VOLUME: 200µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 99
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMVSQPALCI HDVDVVGEPF EDMMAMLDAP VFVVTTQADA VPSGCLVSFA TQTSVQPPSF MIGMPTTSRM VEPASRSDCV AVHVLARRHQ ALAELFGSQA GEEADKFERC SWRAGPYGMP ILDDAAAWFV GKILSRSDVG DHVAYLLEPI AVWAPDAPEE LLYLSDLDIE DFEPGQEEPT RFHKPAEVTR RYGVRFTL
Validated NT Sequence
cgagggtgaa tcgcacgccg tatcggcgcg tcacctcagc gggtttgtgg aaccgggtcg gctcctcctg gccgggttcg aagtcctcaa tatcgaggtc ggacaggtag agcagttctt ccggggcatc gggagcccag acagcaatcg gctccagcag gtaggccacg tggtcgccaa cgtcgctgcg gctcaagatc ttgccgacga accacgcggc cgcatcgtcg agaatcggca tgccgtaggg accggcgcgc cacgagcaac gttcgaattt gtcagcctcc tcgcccgctt ggctgccgaa gagttcggcc agggcctggt gacgacgggc cagcacgtgc accgcgacac agtcggagcg gctcgccggc tccaccatgc ggctggtggt gggcatcccg atcatgaaac ttggtggctg cacgctggtc tgggtggcaa aactgaccag gcaacccgac gggaccgcgt cggcctgggt tgtcaccaca aacaccggcg cgtccagcat cgccatcatg tcttcgaacg gttcaccgac cacatcaaca tcatggatgc acaacgccgg ttgtgacacc atcgaaccag gaccctgggt ctgagcttcc agggtaccca tatgatggtg atggtgatga gccat
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVSQPALCI HDVDVVGEPF EDMMAMLDAP VFVVTTQADA VPSGCLVSFA TQTSVQPPSF MIGMPTTSRM VEPASRSDCV AVHVLARRHQ ALAELFGSQA GEEADKFERC SWRAGPYGMP ILDDAAAWFV GKILSRSDVG DHVAYLLEPI AVWAPDAPEE LLYLSDLDIE DFEPGQEEPT RFHKPAEVTR RYGVRFTLDY
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGTGTCACAA CCGGCGTTGT GCATCCATGA TGTTGATGTG GTCGGTGAAC CGTTCGAAGA CATGATGGCG ATGCTGGACG CGCCGGTGTT TGTGGTGACA ACCCAGGCCG ACGCGGTCCC GTCGGGTTGC CTGGTCAGTT TTGCCACCCA GACCAGCGTG CAGCCACCAA GTTTCATGAT CGGGATGCCC ACCACCAGCC GCATGGTGGA GCCGGCGAGC CGCTCCGACT GTGTCGCGGT GCACGTGCTG GCCCGTCGTC ACCAGGCCCT GGCCGAACTC TTCGGCAGCC AAGCGGGCGA GGAGGCTGAC AAATTCGAAC GTTGCTCGTG GCGCGCCGGT CCCTACGGCA TGCCGATTCT CGACGATGCG GCCGCGTGGT TCGTCGGCAA GATCTTGAGC CGCAGCGACG TTGGCGACCA CGTGGCCTAC CTGCTGGAGC CGATTGCTGT CTGGGCTCCC GATGCCCCGG AAGAACTGCT CTACCTGTCC GACCTCGATA TTGAGGACTT CGAACCCGGC CAGGAGGAGC CGACCCGGTT CCACAAACCC GCTGAGGTGA CGCGCCGATA CGGCGTGCGA TTCACCCTCG ACTATAAACA GCACGAACAA GTTCTGCAGC CAAGCTTCTC GAGGATCCGG CTGCTAACAA AGCCCGAAAG GAAGCTGAGT TGGCTGCTGC CACCGCTGAG CAATAACTAG CATAACCCCT TGGGGCCTCT AAACGGGTCT TGAGGGGTTT TTTGCTGAAA GGAGGAACTA TATCCGGATA TCCACAGGAC GGGTGTGGTC GCCATGATCG CGTAGTCGAT AGTGGCTCCA AGTAGCGAAG CGAGCAGGAC TGGGCGGCGG CCAAAGCGGT CGGACAGTGC TCCGAGAACG GGTGCGCATA GAAATTGCAT CAACGCATAT AGCGCTAGCA GCACGCCATA GTGACTGGCG ATGCTGTCGG AATGGACGAT ATCCCGCAAG AGGCCCGGCA GTACCGGCAT AACCAAGCCT ATGCCTACAG CATCCAGGGT GACGGTGCCG AGGATGACGA TGAGCGCATT GTTAGATTTC ATACACGGTG CCTGACTGCG TTAGCAATTT AACTGTGATA AACTACCGCA TTAAAGCTTA TCGATGATAA GCTGTCAAAC ATGAGAA