MymaA.00249.a

Thymidylate synthase

CENTER ID: MymaA.00249.a
ORGANISM: Mycobacterium marinum ATCC BAA-535 / M
ASSOCIATED DISEASE: Cutaneous ulcerations, deep abscesses
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MymaA.00249.a.A1.GE29975 Full length( MymaA.00249.a ) 1 266
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MymaA.00249.a.A1.PS00857 Full length( MymaA.00249.a ) 1 266

External Resources

RESOURCE REFERENCE ID
OrthoMCL: OG5_127385
PATRIC ID: fig|216594.6.peg.2090
RefSeq: YP_001850261.1
UniProt: B2HKY5

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MPIATPYEDL LRRVLETGTA KSDRTGTGTR SLFGQQIRYD LGCGFPLLTT KKVHFKSVVY ELLWFLRGDS NVGWLQQHGV TIWDEWASET GDLGPIYGVQ WRSWPTPSGE HIDQISAALE LLRTDPDSRR IIVSAWNVGQ IPQMALPPCH AFFQFYVADG RLSCQLYQRS ADLFLGVPFN IASYALLTHM MAAQAGLSVG EFIWTGGDCH IYGNHVEQVT EQLRREPRPY PKLSLSQRDS IFDYTYEDVV VQDYDPHPAI KAPVAV
NT Sequence
GTGCCGATCG CGACGCCTTA CGAGGATCTG TTGCGCAGGG TGCTCGAAAC GGGCACCGCC AAATCCGACC GCACCGGCAC CGGGACCCGC AGCCTGTTCG GCCAGCAGAT CCGGTACGAC CTCGGTTGCG GTTTCCCCTT GCTGACCACC AAGAAGGTGC ACTTCAAGTC GGTCGTTTAT GAGTTGCTGT GGTTTCTGCG CGGCGACTCC AACGTGGGCT GGTTGCAGCA GCACGGTGTG ACCATCTGGG ACGAATGGGC CAGCGAAACA GGCGATCTCG GCCCAATCTA CGGAGTTCAG TGGCGATCCT GGCCGACTCC GTCGGGTGAG CACATCGACC AGATCAGTGC CGCATTGGAA CTGCTGCGTA CCGACCCCGA TTCGCGGCGC ATCATCGTGT CGGCCTGGAA TGTCGGCCAG ATCCCGCAAA TGGCGCTGCC GCCGTGTCAC GCCTTCTTCC AGTTCTACGT CGCCGACGGC CGCCTGAGCT GCCAGCTGTA TCAGCGCAGT GCCGACCTGT TTCTGGGGGT GCCCTTCAAC ATCGCCAGCT ACGCCCTGCT CACCCACATG ATGGCCGCTC AGGCCGGTCT TTCGGTCGGC GAGTTCATCT GGACCGGCGG CGATTGCCAC ATCTATGGCA ACCACGTCGA ACAGGTCACC GAGCAACTGC GTCGCGAGCC TCGGCCCTAT CCGAAACTAA GCTTGTCGCA ACGGGATTCG ATCTTCGACT ACACCTACGA GGACGTCGTC GTGCAGGATT ATGATCCCCA TCCCGCGATC AAAGCTCCCG TCGCTGTA
Details for MymaA.00249.a.A1.GE29975
HARVESTED ON: 7/13/2010
SEQUENCED ON: 8/20/2010
EXPECTED MW: 32kDa
OBSERVED MW: 32kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMVPIATPYE DLLRRVLETG TAKSDRTGTG TRSLFGQQIR YDLGCGFPLL TTKKVHFKSV VYELLWFLRG DSNVGWLQQH GVTIWDEWAS ETGDLGPIYG VQWRSWPTPS GEHIDQISAA LELLRTDPDS RRIIVSAWNV GQIPQMALPP CHAFFQFYVA DGRLSCQLYQ RSADLFLGVP FNIASYALLT HMMAAQAGLS VGEFIWTGGD CHIYGNHVEQ VTEQLRREPR PYPKLSLSQR DSIFDYTYED VVVQDYDPHP AIKAPVA
Validated NT Sequence
ttattaagcg acgggagctt tgatcgcggg atggggatca taatcctgca cgacgacgtc ctcgtaggtg tagtcgaaga tcgaatcccg ttgcgacaag cttagtttcg gatagggccg aggctcgcga cgcagttgct cggtgacctg ttcgacgtgg ttgccataga tgtggcaatc gccgccggtc cagatgaact cgccgaccga aagaccggcc tgagcggcca tcatgtgggt gagcagggcg tagctggcga tgttgaaggg cacccccaga aacaggtcgg cactgcgctg atacagctgg cagctcaggc ggccgtcggc gacgtagaac tggaagaagg cgtgacacgg cggcagcgcc atttgcggga tctggccgac attccaggcc gacacgatga tgcgccgcga atcggggtcg gtacgcagca gttccaatgc ggcactgatc tggtcgatgt gctcacccga cggagtcggc caggatcgcc actgaactcc gtagattggg ccgagatcgc ctgtttcgct ggcccattcg tcccagatgg tcacaccgtg ctgctgcaac cagcccacgt tggagtcgcc gcgcagaaac cacagcaact cataaacgac cgacttgaag tgcaccttct tggtggtcag caaggggaaa ccgcaaccga ggtcgtaccg gatctgctgg ccgaacaggc tgcgggtccc ggtgccggtg cggtcggatt tggcggtgcc cgtttcgagc accctgcgca acagatcctc gtaaggcgtc gcgatcggca ccatcgaacc aggaccctgg gtctgagctt ccagggtacc catatgatgg tgatggtgat gagccat
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVPIATPYE DLLRRVLETG TAKSDRTGTG TRSLFGQQIR YDLGCGFPLL TTKKVHFKSV VYELLWFLRG DSNVGWLQQH GVTIWDEWAS ETGDLGPIYG VQWRSWPTPS GEHIDQISAA LELLRTDPDS RRIIVSAWNV GQIPQMALPP CHAFFQFYVA DGRLSCQLYQ RSADLFLGVP FNIASYALLT HMMAAQAGLS VGEFIWTGGD CHIYGNHVEQ VTEQLRREPR PYPKLSLSQR DSIFDYTYED VVVQDYDPHP AIKAPVA
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtgccgatc gcgacgcctt acgaggatct gttgcgcagg gtgctcgaaa cgggcaccgc caaatccgac cgcaccggca ccgggacccg cagcctgttc ggccagcaga tccggtacga cctcggttgc ggtttcccct tgctgaccac caagaaggtg cacttcaagt cggtcgttta tgagttgctg tggtttctgc gcggcgactc caacgtgggc tggttgcagc agcacggtgt gaccatctgg gacgaatggg ccagcgaaac aggcgatctc ggcccaatct acggagttca gtggcgatcc tggccgactc cgtcgggtga gcacatcgac cagatcagtg ccgcattgga actgctgcgt accgaccccg attcgcggcg catcatcgtg tcggcctgga atgtcggcca gatcccgcaa atggcgctgc cgccgtgtca cgccttcttc cagttctacg tcgccgacgg ccgcctgagc tgccagctgt atcagcgcag tgccgacctg tttctggggg tgcccttcaa catcgccagc tacgccctgc tcacccacat gatggccgct caggccggtc tttcggtcgg cgagttcatc tggaccggcg gcgattgcca catctatggc aaccacgtcg aacaggtcac cgagcaactg cgtcgcgagc ctcggcccta tccgaaacta agcttgtcgc aacgggattc gatcttcgac tacacctacg aggacgtcgt cgtgcaggat tatgatcccc atcccgcgat caaagctccc gtcgctaaac agcacgaaca agttctgcag ccaagcttct cgaggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaa
Details for MymaA.00249.a.A1.PS00857
PURIFICATION DATe: 11/5/2010
CONCENTRATION: 31.61mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 6
VIAL VOLUME: 200µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMVPIATPYE DLLRRVLETG TAKSDRTGTG TRSLFGQQIR YDLGCGFPLL TTKKVHFKSV VYELLWFLRG DSNVGWLQQH GVTIWDEWAS ETGDLGPIYG VQWRSWPTPS GEHIDQISAA LELLRTDPDS RRIIVSAWNV GQIPQMALPP CHAFFQFYVA DGRLSCQLYQ RSADLFLGVP FNIASYALLT HMMAAQAGLS VGEFIWTGGD CHIYGNHVEQ VTEQLRREPR PYPKLSLSQR DSIFDYTYED VVVQDYDPHP AIKAPVA
Validated NT Sequence
ttattaagcg acgggagctt tgatcgcggg atggggatca taatcctgca cgacgacgtc ctcgtaggtg tagtcgaaga tcgaatcccg ttgcgacaag cttagtttcg gatagggccg aggctcgcga cgcagttgct cggtgacctg ttcgacgtgg ttgccataga tgtggcaatc gccgccggtc cagatgaact cgccgaccga aagaccggcc tgagcggcca tcatgtgggt gagcagggcg tagctggcga tgttgaaggg cacccccaga aacaggtcgg cactgcgctg atacagctgg cagctcaggc ggccgtcggc gacgtagaac tggaagaagg cgtgacacgg cggcagcgcc atttgcggga tctggccgac attccaggcc gacacgatga tgcgccgcga atcggggtcg gtacgcagca gttccaatgc ggcactgatc tggtcgatgt gctcacccga cggagtcggc caggatcgcc actgaactcc gtagattggg ccgagatcgc ctgtttcgct ggcccattcg tcccagatgg tcacaccgtg ctgctgcaac cagcccacgt tggagtcgcc gcgcagaaac cacagcaact cataaacgac cgacttgaag tgcaccttct tggtggtcag caaggggaaa ccgcaaccga ggtcgtaccg gatctgctgg ccgaacaggc tgcgggtccc ggtgccggtg cggtcggatt tggcggtgcc cgtttcgagc accctgcgca acagatcctc gtaaggcgtc gcgatcggca ccatcgaacc aggaccctgg gtctgagctt ccagggtacc catatgatgg tgatggtgat gagccat
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVPIATPYE DLLRRVLETG TAKSDRTGTG TRSLFGQQIR YDLGCGFPLL TTKKVHFKSV VYELLWFLRG DSNVGWLQQH GVTIWDEWAS ETGDLGPIYG VQWRSWPTPS GEHIDQISAA LELLRTDPDS RRIIVSAWNV GQIPQMALPP CHAFFQFYVA DGRLSCQLYQ RSADLFLGVP FNIASYALLT HMMAAQAGLS VGEFIWTGGD CHIYGNHVEQ VTEQLRREPR PYPKLSLSQR DSIFDYTYED VVVQDYDPHP AIKAPVA
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGTGCCGATC GCGACGCCTT ACGAGGATCT GTTGCGCAGG GTGCTCGAAA CGGGCACCGC CAAATCCGAC CGCACCGGCA CCGGGACCCG CAGCCTGTTC GGCCAGCAGA TCCGGTACGA CCTCGGTTGC GGTTTCCCCT TGCTGACCAC CAAGAAGGTG CACTTCAAGT CGGTCGTTTA TGAGTTGCTG TGGTTTCTGC GCGGCGACTC CAACGTGGGC TGGTTGCAGC AGCACGGTGT GACCATCTGG GACGAATGGG CCAGCGAAAC AGGCGATCTC GGCCCAATCT ACGGAGTTCA GTGGCGATCC TGGCCGACTC CGTCGGGTGA GCACATCGAC CAGATCAGTG CCGCATTGGA ACTGCTGCGT ACCGACCCCG ATTCGCGGCG CATCATCGTG TCGGCCTGGA ATGTCGGCCA GATCCCGCAA ATGGCGCTGC CGCCGTGTCA CGCCTTCTTC CAGTTCTACG TCGCCGACGG CCGCCTGAGC TGCCAGCTGT ATCAGCGCAG TGCCGACCTG TTTCTGGGGG TGCCCTTCAA CATCGCCAGC TACGCCCTGC TCACCCACAT GATGGCCGCT CAGGCCGGTC TTTCGGTCGG CGAGTTCATC TGGACCGGCG GCGATTGCCA CATCTATGGC AACCACGTCG AACAGGTCAC CGAGCAACTG CGTCGCGAGC CTCGGCCCTA TCCGAAACTA AGCTTGTCGC AACGGGATTC GATCTTCGAC TACACCTACG AGGACGTCGT CGTGCAGGAT TATGATCCCC ATCCCGCGAT CAAAGCTCCC GTCGCTAAAC AGCACGAACA AGTTCTGCAG CCAAGCTTCT CGAGGATCCG GCTGCTAACA AAGCCCGAAA GGAAGCTGAG TTGGCTGCTG CCACCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC TAAACGGGTC TTGAGGGGTT TTTTGCTGAA AGGAGGAACT ATATCCGGAT ATCCACAGGA CGGGTGTGGT CGCCATGATC GCGTAGTCGA TAGTGGCTCC AAGTAGCGAA GCGAGCAGGA CTGGGCGGCG GCCAAAGCGG TCGGACAGTG CTCCGAGAAC GGGTGCGCAT AGAAATTGCA TCAACGCATA TAGCGCTAGC AGCACGCCAT AGTGACTGGC GATGCTGTCG GAATGGACGA TATCCCGCAA GAGGCCCGGC AGTACCGGCA TAACCAAGCC TATGCCTACA GCATCCAGGG TGACGGTGCC GAGGATGACG ATGAGCGCAT TGTTAGATTT CATACACGGT GCCTGACTGC GTTAGCAATT TAACTGTGAT AAACTACCGC ATTAAAGCTT ATCGATGATA AGCTGTCAAA CATGAGAA