MymaA.00168.a

2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase (IspD)

CENTER ID: MymaA.00168.a
ORGANISM: Mycobacterium marinum ATCC BAA-535 / M
ASSOCIATED DISEASE: Cutaneous ulcerations, deep abscesses
CURRENT STATUS: soluble
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

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Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MymaA.00168.a.A1.GE29957 Full length( MymaA.00168.a ) 1 227
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|216594.6.peg.5445
RefSeq: YP_001853341.1
UniProt: B2HJ23

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MAGGTQGVAA IVPAAGSGQR LAAGVPKAFY QLEGQTLVER AVRGLLESGV VDTVVVAVPP DRTDEAKLIL GREATIVAGG ADRTESVSRA LSALSAGVSP EFVLVHDAAR ALTPPALVVR LVDMLRAGHA AVVPVLPLTD TIKAVDANGV VLGTPERAGL RAVQTPQGFS TELLLRAYRH AGVAAFTDDA SLVEHVGGQV HVVAGDPLAF KITNRLDLLL AHAVVRG
NT Sequence
GTGGCCGGGG GAACCCAGGG CGTCGCCGCG ATTGTTCCGG CCGCAGGGTC GGGTCAGCGG CTCGCGGCTG GCGTGCCTAA GGCGTTCTAT CAACTCGAAG GTCAAACTCT GGTTGAGCGA GCCGTCAGGG GCTTGCTCGA ATCCGGGGTG GTGGACACCG TCGTGGTGGC GGTACCCCCA GATCGCACCG ACGAGGCAAA GCTGATCCTG GGCCGCGAGG CCACCATTGT GGCCGGCGGC GCCGATCGCA CCGAAAGTGT GAGCCGGGCC TTGTCCGCCT TGTCTGCCGG CGTCTCGCCG GAATTCGTAC TTGTCCATGA CGCTGCGCGG GCGCTGACGC CGCCGGCACT GGTCGTGCGG TTGGTCGACA TGTTGCGGGC TGGCCATGCC GCGGTCGTAC CGGTGCTGCC GCTCACTGAC ACAATCAAGG CTGTGGATGC CAATGGGGTG GTTCTGGGTA CGCCGGAACG CGCGGGGCTG AGGGCGGTGC AGACCCCGCA GGGGTTCTCC ACCGAACTGC TGCTGCGCGC CTACCGACAC GCGGGCGTCG CCGCCTTCAC CGATGACGCA TCGCTGGTTG AGCATGTCGG TGGGCAGGTG CACGTGGTTG CCGGCGACCC GCTGGCGTTC AAGATCACCA ACCGTCTCGA TCTGTTGCTG GCCCACGCGG TTGTGCGCGG G
Details for MymaA.00168.a.A1.GE29957
HARVESTED ON: 7/13/2010
SEQUENCED ON: 8/20/2010
EXPECTED MW: 25kDa
OBSERVED MW: 26kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 99
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMVAGGTQGV AAIVPAAGSG QRLAAGVPKA FYQLEGQTLV ERAVRGLLES GVVDTVVVAV PPDRTDEAKL ILGREATIVA GGADRTESVS RALSALSAGV SPEFVLVHDA ARALTPPALV VRLVDMLRAG HAAVXXVLPL TDTIKAVDAN GVVLGTPERA GLRAVQTPQG FSTELLLRAY RHAGVAAFTD DASLVEHVGG QVHVVAGDPL AFKITNRLDL LLAHAVVR
Validated NT Sequence
ttattagcgc acaaccgcgt gggccagcaa cagatcgaga cggttggtga tcttgaacgc cagcgggtcg ccggcaacca cgtgcacctg cccaccgaca tgctcaacca gcgatgcgtc atcggtgaag gcggcgacgc ccgcgtgtcg gtaggcgcgc agcagcagtt cggtggagaa cccctgcggg gtctgcaccg ccctcagccc cgcgcgttcc ggcgtaccca gaaccacccc attggcatcc acagccttga ttgtgtcagt gagcggcagc acnnnnncga ccgcggcatg gccagcccgc aacatgtcga ccaaccgcac gaccagtgcc ggcggcgtca gcgcccgcgc agcgtcatgg acaagtacga attccggcga gacgccggca gacaaggcgg acaaggcccg gctcacactt tcggtgcgat cggcgccgcc ggccacaatg gtggcctcgc ggcccaggat cagctttgcc tcgtcggtgc gatctggggg taccgccacc acgacggtgt ccaccacccc ggattcgagc aagcccctga cggctcgctc aaccagagtt tgaccttcga gttgatagaa cgccttaggc acgccagccg cgagccgctg acccgaccct gcggccggaa caatcgcggc gacgccctgg gttcccccgg ccaccatcga accaggaccc tgggtctgag cttccagggt acccatatga tggtgatggt gatgagccat
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVAGGTQGV AAIVPAAGSG QRLAAGVPKA FYQLEGQTLV ERAVRGLLES GVVDTVVVAV PPDRTDEAKL ILGREATIVA GGADRTESVS RALSALSAGV SPEFVLVHDA ARALTPPALV VRLVDMLRAG HAAVVPVLPL TDTIKAVDAN GVVLGTPERA GLRAVQTPQG FSTELLLRAY RHAGVAAFTD DASLVEHVGG QVHVVAGDPL AFKITNRLDL LLAHAVVR
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtggccggg ggaacccagg gcgtcgccgc gattgttccg gccgcagggt cgggtcagcg gctcgcggct ggcgtgccta aggcgttcta tcaactcgaa ggtcaaactc tggttgagcg agccgtcagg ggcttgctcg aatccggggt ggtggacacc gtcgtggtgg cggtaccccc agatcgcacc gacgaggcaa agctgatcct gggccgcgag gccaccattg tggccggcgg cgccgatcgc accgaaagtg tgagccgggc cttgtccgcc ttgtctgccg gcgtctcgcc ggaattcgta cttgtccatg acgctgcgcg ggcgctgacg ccgccggcac tggtcgtgcg gttggtcgac atgttgcggg ctggccatgc cgcggtcgta ccggtgctgc cgctcactga cacaatcaag gctgtggatg ccaatggggt ggttctgggt acgccggaac gcgcggggct gagggcggtg cagaccccgc aggggttctc caccgaactg ctgctgcgcg cctaccgaca cgcgggcgtc gccgccttca ccgatgacgc atcgctggtt gagcatgtcg gtgggcaggt gcacgtggtt gccggcgacc cgctggcgtt caagatcacc aaccgtctcg atctgttgct ggcccacgcg gttgtgcgca aacagcacga acaagttctg cagccaagct tctcgaggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga a