MymaA.00148.a

Integrase/recombinase, XerD

CENTER ID: MymaA.00148.a
ORGANISM: Mycobacterium marinum ATCC BAA-535 / M
ASSOCIATED DISEASE: Cutaneous ulcerations, deep abscesses
CURRENT STATUS: soluble
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MymaA.00148.a.A1.GE29953 Full length( MymaA.00148.a ) 1 313
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|216594.6.peg.2669
RefSeq: YP_001850812.1
UniProt: B2HR71

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSTLTLDTQL QGYLDHLAIE RGVAANTLSS YRRDLRRYSK HLEDRGITDL AKVGEDDVSE FLVALRRGDP ESGVLGLSAV SAARALIAVR GLHRFAAAEG LAALDVARAV RPPTPGRRLP KSLTIDEVLA LLEGAGGDNP ADGPLTLRNR ALLELLYSTG SRISEAVGLD VDDIDTQART VLLQGKGGKQ RLVPVGRPAV QALDAYLVRG RPELARRGRG TPAIFLNARG GRLSRQSAWQ VLQDAAERAG ITSGVSPHML RHSFATHLLE GGADVRVVQE LLGHASVTTT QIYTLVTVHA LREVWAGAHP RAT
NT Sequence
GTGAGCACCC TGACCCTGGA CACGCAGTTG CAGGGTTATC TCGACCATTT AGCGATCGAA CGTGGCGTTG CCGCCAACAC GTTGAGCTCC TACCGGCGGG ACCTGCGCCG CTACTCCAAG CACCTCGAAG ACCGGGGGAT CACCGATTTA GCCAAGGTGG GCGAGGACGA TGTGAGCGAG TTCCTGGTCG CCCTGCGACG GGGAGATCCC GAATCCGGAG TGCTCGGGCT GTCGGCGGTG TCGGCGGCCC GAGCGCTGAT TGCGGTGCGC GGGCTGCATC GGTTCGCGGC CGCGGAGGGA CTGGCCGCGC TGGATGTGGC GCGCGCCGTG CGTCCGCCCA CACCGGGCCG GAGGCTGCCC AAGAGTCTGA CCATCGATGA GGTGCTGGCC CTGCTCGAAG GTGCCGGTGG TGACAATCCG GCGGACGGCC CGCTGACGCT GCGCAACCGG GCATTGCTGG AGCTGTTGTA TTCCACCGGG TCTCGGATCT CCGAGGCGGT CGGACTCGAT GTCGACGATA TCGATACGCA GGCCAGGACG GTGTTGTTAC AGGGCAAGGG CGGTAAGCAG CGGCTGGTGC CGGTGGGGCG TCCCGCGGTA CAAGCCTTGG ACGCCTATCT GGTGCGCGGA CGTCCGGAGC TCGCCCGCCG CGGCCGCGGC ACCCCGGCGA TTTTCCTCAA CGCGCGCGGC GGTCGCCTGT CGCGCCAAAG CGCTTGGCAG GTGTTGCAGG ATGCGGCCGA ACGTGCCGGC ATCACCTCGG GGGTATCGCC GCACATGCTG CGCCATTCGT TTGCGACTCA TCTGCTGGAA GGCGGCGCGG ATGTCCGGGT GGTGCAGGAG CTTCTGGGTC ATGCCTCGGT GACAACGACG CAGATCTACA CGCTGGTCAC CGTGCACGCG CTGCGCGAAG TGTGGGCCGG CGCGCACCCC CGGGCCACC
Details for MymaA.00148.a.A1.GE29953
HARVESTED ON: 7/13/2010
SEQUENCED ON: 8/20/2010
EXPECTED MW: 35kDa
OBSERVED MW: 35kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Few (1-10)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 29
PERCENT COVERAGE: 35
Validated AA Sequence
MSTISIRRPG RCCYRARAVS SGWCRWGVPR YKPWTPIWCA DVRSSPAAAA APRRFSSTRA AVACRAKALG RCCRMRPNVP ASPRGYRRTC CAIRLRLICW KAARMSGWCR SFWVMPR
Validated NT Sequence
cgcgccggcc cacacttcgc gcagcgcgtg cacggtgacc agcgtgtaga tctgcgtcgt tgtcaccgag gcatgaccca gaagctcctg caccacccgg acatccgcgc cgccttccag cagatgagtc gcaaacgaat ggcgcagcat gtgcggcgat acccccgagg tgatgccggc acgttcggcc gcatcctgca acacctgcca agcgctttgg cgcgacaggc gaccgccgcg cgcgttgagg aaaatcgccg gggtgccgcg gccgcggcgg gcgagctccg gacgtccgcg caccagatag gcgtccaagg cttgtaccgc gggacgcccc accggcacca gccgctgctt accgcccttg ccctgtaaca acaccgtcct ggcctgcgta tcgatatcgt cgacatcgag tccgaccgcc tcggagatcc gagacccggt ggaatacaac agctccagca atgcccggtt gcgcagcgtc agcgggccgt ccgccggatt gtcaccaccg gcaccttcga gcagggccag cacctcatcg atggtcagac tcttgggcag cctccggccc ggtgtgggcg gacgcacggc gcgcgccaca tccagcgcgg ccagtccctc cgcggccgcg aaccgatgca gcccgcgcac cgcaatcagc gctcgggccg ccgacaccgc cgacagcccg agcactccgg attcgggatc tccccgtcgc agggcgacca ggaactcgct cacatcgtcc tcgcccacct tggctaaatc ggtgatcccc cggtcttcga ggtgcttgga gtagcggcgc aggtcc
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVSTLTLDT QLQGYLDHLA IERGVAANTL SSYRRDLRRY SKHLEDRGIT DLAKVGEDDV SEFLVALRRG DPESGVLGLS AVSAARALIA VRGLHRFAAA EGLAALDVAR AVRPPTPGRR LPKSLTIDEV LALLEGAGGD NPADGPLTLR NRALLELLYS TGSRISEAVG LDVDDIDTQA RTVLLQGKGG KQRLVPVGRP AVQALDAYLV RGRPELARRG RGTPAIFLNA RGGRLSRQSA WQVLQDAAER AGITSGVSPH MLRHSFATHL LEGGADVRVV QELLGHASVT TTQIYTLVTV HALREVWAGA HPRA
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtgagcacc ctgaccctgg acacgcagtt gcagggttat ctcgaccatt tagcgatcga acgtggcgtt gccgccaaca cgttgagctc ctaccggcgg gacctgcgcc gctactccaa gcacctcgaa gaccggggga tcaccgattt agccaaggtg ggcgaggacg atgtgagcga gttcctggtc gccctgcgac ggggagatcc cgaatccgga gtgctcgggc tgtcggcggt gtcggcggcc cgagcgctga ttgcggtgcg cgggctgcat cggttcgcgg ccgcggaggg actggccgcg ctggatgtgg cgcgcgccgt gcgtccgccc acaccgggcc ggaggctgcc caagagtctg accatcgatg aggtgctggc cctgctcgaa ggtgccggtg gtgacaatcc ggcggacggc ccgctgacgc tgcgcaaccg ggcattgctg gagctgttgt attccaccgg gtctcggatc tccgaggcgg tcggactcga tgtcgacgat atcgatacgc aggccaggac ggtgttgtta cagggcaagg gcggtaagca gcggctggtg ccggtggggc gtcccgcggt acaagccttg gacgcctatc tggtgcgcgg acgtccggag ctcgcccgcc gcggccgcgg caccccggcg attttcctca acgcgcgcgg cggtcgcctg tcgcgccaaa gcgcttggca ggtgttgcag gatgcggccg aacgtgccgg catcacctcg ggggtatcgc cgcacatgct gcgccattcg tttgcgactc atctgctgga aggcggcgcg gatgtccggg tggtgcagga gcttctgggt catgcctcgg tgacaacgac gcagatctac acgctggtca ccgtgcacgc gctgcgcgaa gtgtgggccg gcgcgcaccc ccgggccaaa cagcacgaac aagttctgca gccaagcttc tcgaggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaa