SSGCID: Target Page

MymaA.00013.a

Holliday junction ATP-dependent DNA helicase RuvB

CENTER ID:	MymaA.00013.a
ORGANISM:	Mycobacterium marinum ATCC BAA-535 / M
ASSOCIATED DISEASE:	Cutaneous ulcerations, deep abscesses
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	False
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MymaA.00013.a.B1.GE40290	Full length(MymaA.00013.a)		1	345

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|216594.6.peg.2255
UniProt:	B2HN63

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MSTDPDEREV SPALTVGDGD VDVSLRPRSL REFIGQPRVR EQLQLVIQGA KNRGGTPDHI LLSGPPGLGK TSLAMIIAAE LGSSLRMTSG PALERAGDLA AMLSNLVEHD VLFIDEIHRI ARPAEEMLYL AMEDFRVDVV VGKGPGATSI PLDVAPFTLV GATTRSGALT GPLRDRFGFT AHMDFYEPAE LERVLVRSAG ILGIQLGADA GAEIARRSRG TPRIANRLLR RVRDFAEVRA DGVITRDVAK AALAVYDVDE LGLDRLDRAV LSALTRSFSG GPVGVSTLAV AVGEEASTVE EVCEPFLVRA GMVARTPRGR VATALAWTHL GMTPPAGANQ PGLFE

NT Sequence

ATGAGCACCG ATCCCGATGA GCGCGAGGTC TCACCGGCGC TGACCGTTGG CGATGGCGAT GTCGACGTCA GCCTGCGGCC GCGCTCATTG CGTGAGTTCA TCGGCCAGCC ACGGGTGCGC GAACAACTTC AGCTGGTGAT TCAGGGCGCC AAGAATCGCG GCGGCACACC GGATCACATT CTGTTGTCCG GTCCGCCCGG GCTCGGGAAG ACCTCGCTGG CGATGATCAT CGCCGCCGAG CTTGGTTCCT CCCTGCGGAT GACATCGGGT CCCGCGCTCG AGCGGGCCGG CGATCTGGCC GCGATGTTGT CCAACCTGGT CGAGCATGAC GTGCTGTTCA TCGACGAGAT TCACCGCATC GCCCGGCCCG CCGAGGAGAT GCTCTATCTG GCCATGGAGG ATTTCCGGGT CGACGTGGTG GTCGGCAAAG GGCCGGGGGC GACATCGATT CCGCTGGACG TCGCGCCGTT CACCCTGGTC GGGGCCACCA CCCGATCCGG CGCGTTGACG GGGCCGCTGC GTGACCGGTT CGGTTTCACC GCACACATGG ATTTTTATGA GCCCGCCGAG CTGGAGCGGG TGCTGGTCCG TTCCGCCGGA ATTCTGGGCA TCCAACTCGG TGCGGACGCG GGGGCCGAGA TCGCCCGCCG GTCACGCGGG ACTCCGCGGA TCGCCAACCG GCTGTTGCGC CGCGTCCGTG ACTTTGCCGA GGTTCGTGCC GACGGGGTGA TCACCCGTGA TGTCGCCAAG GCGGCGCTGG CGGTTTACGA CGTCGACGAG CTGGGTCTGG ATCGGCTGGA CCGTGCCGTG CTTTCCGCCC TGACCCGCAG TTTCAGTGGC GGTCCGGTAG GTGTGTCGAC GCTGGCGGTG GCGGTGGGGG AGGAGGCCAG CACTGTGGAG GAGGTGTGTG AGCCGTTCCT GGTCCGTGCC GGCATGGTCG CGCGCACTCC ACGCGGCCGG GTGGCCACCG CACTGGCCTG GACTCACCTG GGCATGACGC CACCGGCGGG AGCGAATCAG CCGGGGCTGT TCGAG

Details for MymaA.00013.a.B1.GE40290

HARVESTED ON:	6/13/2016
SEQUENCED ON:	6/16/2016
EXPECTED MW:	38kDa
OBSERVED MW:	38kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY:	100
PERCENT COVERAGE:	97

Validated AA Sequence

MAHHHHHHMS TDPDEREVSP ALTVGDGDVD VSLRPRSLRE FIGQPRVREQ LQLVIQGAKN RGGTPDHILL SGPPGLGKTS LAMIIAAELG SSLRMTSGPA LERAGDLAAM LSNLVEHDVL FIDEIHRIAR PAEEMLYLAM EDFRVDVVVG KGPGATSIPL DVAPFTLVGA TTRSGALTGP LRDRFGFTAH MDFYEPAELE RVLVRSAGIL GIQLGADAGA EIARRSRGTP RIANRLLRRV RDFAEVRADG VITRDVAKAA LAVYDVDELG LDRLDRAVLS ALTRSFSGGP VGVSTLAVAV GEEASTVEEV CEPFLVRAGM VARTPRGRVA TALAWTHLGM TPPA

Validated NT Sequence

tcccgccggt ggcgtcatgc ccaggtgagt ccaggccagt gcggtggcca cccggccgcg tggagtgcgc gcgaccatgc cggcacggac caggaacggc tcacacacct cctccacagt gctggcctcc tcccccaccg ccaccgccag cgtcgacaca cctaccggac cgccactgaa actgcgggtc agggcggaaa gcacggcacg gtccagccga tccagaccca gctcgtcgac gtcgtaaacc gccagcgccg ccttggcgac atcacgggtg atcaccccgt cggcacgaac ctcggcaaag tcacggacgc ggcgcaacag ccggttggcg atccgcggag tcccgcgtga ccggcgggcg atctcggccc ccgcgtccgc accgagttgg atgcccagaa ttccggcgga acggaccagc acccgctcca gctcggcggg ctcataaaaa tccatgtgtg cggtgaaacc gaaccggtca cgcagcggcc ccgtcaacgc gccggatcgg gtggtggccc cgaccagggt gaacggcgcg acgtccagcg gaatcgatgt cgcccccggc cctttgccga ccaccacgtc gacccggaaa tcctccatgg ccagatagag catctcctcg gcgggccggg cgatgcggtg aatctcgtcg atgaacagca cgtcatgctc gaccaggttg gacaacatcg cggccagatc gccggcccgc tcgagcgcgg gacccgatgt catccgcagg gaggaaccaa gctcggcggc gatgatcatc gccagcgagg tcttcccgag cccgggcgga ccggacaaca gaatgtgatc cggtgtgccg ccgcgattct tggcgccctg aatcaccagc tgaagttgtt cgcgcacccg tggctggccg atgaactcac gcaatgagcg cggccgcagg ctgacgtcga catcgccatc gccaacggtc agcgccggtg agacctcgcg ctcatcggga tcggtgctca tatggtggtg gtggtggtga gccat

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgagca ccgatcccga tgagcgcgag gtctcaccgg cgctgaccgt tggcgatggc gatgtcgacg tcagcctgcg gccgcgctca ttgcgtgagt tcatcggcca gccacgggtg cgcgaacaac ttcagctggt gattcagggc gccaagaatc gcggcggcac accggatcac attctgttgt ccggtccgcc cgggctcggg aagacctcgc tggcgatgat catcgccgcc gagcttggtt cctccctgcg gatgacatcg ggtcccgcgc tcgagcgggc cggcgatctg gccgcgatgt tgtccaacct ggtcgagcat gacgtgctgt tcatcgacga gattcaccgc atcgcccggc ccgccgagga gatgctctat ctggccatgg aggatttccg ggtcgacgtg gtggtcggca aagggccggg ggcgacatcg attccgctgg acgtcgcgcc gttcaccctg gtcggggcca ccacccgatc cggcgcgttg acggggccgc tgcgtgaccg gttcggtttc accgcacaca tggattttta tgagcccgcc gagctggagc gggtgctggt ccgttccgcc ggaattctgg gcatccaact cggtgcggac gcgggggccg agatcgcccg ccggtcacgc gggactccgc ggatcgccaa ccggctgttg cgccgcgtcc gtgactttgc cgaggttcgt gccgacgggg tgatcacccg tgatgtcgcc aaggcggcgc tggcggttta cgacgtcgac gagctgggtc tggatcggct ggaccgtgcc gtgctttccg ccctgacccg cagtttcagt ggcggtccgg taggtgtgtc gacgctggcg gtggcggtgg gggaggaggc cagcactgtg gaggaggtgt gtgagccgtt cctggtccgt gccggcatgg tcgcgcgcac tccacgcggc cgggtggcca ccgcactggc ctggactcac ctgggcatga cgccaccggc gggagcgaat cagccggggc tgttcgagtg agtaagatag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa agctcatcag cgtggtcgtg aagcgattca cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg gcgggaccag agaaaaatca ctcagggtca atgccagcgc ttcgttaata cagatgtagg tgttccacag ggtagccagc agcatcctgc gatgcagatc cggaacataa tggtgcaggg cgctgacttc cgcgtttcca gactttacga aacacggaaa ccgaagacca ttcatgttgt tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga ttcattctgc taaccagtaa ggcaaccccg ccagcctagc cgggtcctca acgacaggag cacgatcatg cgcacccgtg gccaggaccc aacgctgccc gagatgcgcc gcgtgcggct gctggagatg gcggacgcga tggatatgtt ctgccaaggg ttggtttgcg cattcacagt tctccgcaag aattgattgg ctccaattct tggagtggtg aatccgttag cgaggtgccg ccggcttcca ttcaggtcga ggtggcccgg ctccatgcac cgcgacgcaa cgcggggagg cagacaaggt atagggcggc gcctacaatc catgccaacc cgttccatgt gctcgccgag gcggcataaa tcgccgtgac gatcagcggt ccagtgatcg aagttaggct ggtaagagcc gcgagcgatc cttgaagctg tccctgatgg tcgtcatcta cctgcctgga cagcatggcc tgcaacgcgg gcatcccgat gccgccggaa gcgagaagaa tcataatggg gaaggccatc cagcctcgcg tcgcgaacgc cagcaagacg tagcccagcg cgtcggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aat