SSGCID: Target Page

MyleA.20994.a

Acyl-CoA synthetase (FadD32)

CENTER ID:	MyleA.20994.a
ORGANISM:	Mycobacterium leprae Br4923
ASSOCIATED DISEASE:	Hansen's disease, leprosy
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MyleA.20994.a.UX1.GE45895	full length		1	635

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

NOTE: Effective as of September 1, 2026, SSGCID will no longer ship physical clone glycerols. SSGCID will provide amino acid and nucleotide sequences for any clones ordered, which the ordering party can then use to order the clone from a third-party clone manufacturer.

External Resources

RESOURCE	REFERENCE ID
UniProt:	A0A0H3MP82

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MTGEGRMAYH NPFIVNGKIR FPPNTNLVRH VEKWAKVRGD KLAYRFLDFS TGRDGVARDI LWSEFSARNR AVGARLQQVT QPGDRIAILC PQNLDYLISF FGALYSGRIA VPLFDPAEPG HVGRLHAVLD DCTPSTILTT TDSAEGVRKF IRGRFAKERT RVIAVDAVPN EVAATWQQPE ANEETVAYLQ YTSGSTRIPS GVQITHLNLP TNVLQVLNSL QGQEGDRGVS WLPFFHDMGL ITVLLPSVLG HSFTFMTPAA FVRRPGRWIR ELARKPEDTG GTFSAAPNFA FEHAAMRGLP RDDELPLDLS NVKGILNGSE PVSPASMRKF FEAFAPYGLK QTAVKPSYGL AEATLFVSTT PMDESPTVIH VDRYELNNQR FVEVPADAPN AVAQVSAGKV GVSEWAIIVD ADTASELPDG QIGEIWLHGN NLGTGYWGKE EESAQTFNNI LKSRISESRA EGASDDASWV RTGDYGTYFK DHLYIAGRIK DLVIIDGRNH YPQDLEYTAQ ESTKALRVGY VAAFSVPANQ LAQVVLEDPH VGLKFDAEDT SEQLVIVGER AAGTHKLDYQ PIVDDIRAAI AVRHGVAVRD VLLVPAGTIP RTSSGKIGRR ACRAAYIDGS LRSGASSPTV FAAEF

NT Sequence

gtgacaggag agggtcgcat ggcgtaccac aacccgttca ttgtgaatgg aaagatcagg ttcccgccta ataccaacct ggtccgtcac gtcgaaaagt gggcaaaggt tcgcggcgac aagttggctt atcgattcct ggacttctcc accggccgcg atggtgtcgc gcgcgacatt ttgtggtccg aattcagcgc ccgcaaccga gcggtgggcg cccgcttaca acaagtcacc cagccgggtg accgcatcgc catcctatgt ccgcagaacc tggactacct gatctccttc ttcggtgccc tgtattccgg ccggatcgcg gtgccgttgt tcgacccggc cgagccgggg catgtcggcc ggttacacgc agtgctcgac gattgcactc cctcgactat cctaactacc accgactccg ccgaaggtgt ccgcaagttc atccggggtc ggtttgctaa ggagcgaact cgcgttatcg cggttgacgc agtgcccaac gaggttgccg ccacctggca gcagcctgag gcaaacgagg aaaccgtggc ctatctgcag tacacgtcgg gctccacccg cattccgagc ggcgtgcaga tcacccacct gaacctaccc accaatgtat tgcaggtgct gaattccctg caaggacagg aaggcgaccg tggggtcagc tggcttccgt tcttccacga catgggtctt attacggtgc tgctcccgtc ggttcttggt cacagcttta cctttatgac gcccgcggcg ttcgtgcgtc ggcccggtcg ctggattcgc gaacttgcgc gtaagcccga agacaccggc ggcaccttct cggcggcgcc gaacttcgcg tttgaacacg ccgcgatgcg tggactgcct cgagatgacg agttgccact ggatctgagt aacgtcaaag gcatcctcaa cggcagcgag ccggtgtcac cggcgtcgat gcgcaagttt ttcgaagcgt ttgcgccgta cggtttgaag cagaccgccg tcaagccgtc ctacgggcta gccgaagcta cactgtttgt ctcgaccacc ccgatggacg agtcgcccac ggtgatccac gtggaccgct acgagctgaa caatcagcga ttcgtggagg tgcccgctga tgcaccgaac gcggtagccc aggtttccgc gggcaaggtc ggggtgagcg aatgggcgat catcgtcgat gccgacactg ccagtgagtt accagacggg cagatcggtg agatctggct gcacggcaac aacctcggta ccggttattg gggcaaggaa gaagagtccg cccagacctt caacaacatc ctcaaatctc ggatcagtga gtcgcgtgcc gaaggagcct ccgacgacgc gtcgtgggtg cgtaccggag actacggcac ctacttcaag gaccatctct atatagccgg ccggatcaag gacctcgtca tcatcgatgg ccgcaaccat tacccgcagg acctggaata cacggcacag gaatcgacta aggcgttgcg cgttggctac gtagccgcct tctccgtccc cgccaaccaa ttggcccagg tggtgctcga ggacccgcac gtcggcctaa agttcgatgc cgaggacacc tccgagcagt tggtgatcgt cggggagcgc gctgctggca cgcacaagct tgactaccag cccatcgttg acgacatccg cgcggctatc gctgtgaggc atggggtggc ggtgcgtgat gtgctgctgg tgccggccgg aacgattccc cgcacttcca gcggcaagat cggccggcgc gcctgccgcg ccgcctacat tgacggtagt ctgcgcagcg gcgccagctc tccgacggtt ttcgctgctg aattctga

Details for MyleA.20994.a.UX1.GE45895

HARVESTED ON:	4/24/2025
SEQUENCED ON:	data unavailable
EXPECTED MW:	72kDa
OBSERVED MW:	72kDa
ANTIBIOTIC MARKER:	kanamycin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	High Expression
SOLUBLE EXPRESSION LEVEL	Insoluble
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	data unavailable
PERCENT COVERAGE:	data unavailable

Validated AA Sequence

sequence unavailable

Validated NT Sequence

sequence unavailable

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMTGEGRMAY HNPFIVNGKI RFPPNTNLVR HVEKWAKVRG DKLAYRFLDF STGRDGVARD ILWSEFSARN RAVGARLQQV TQPGDRIAIL CPQNLDYLIS FFGALYSGRI AVPLFDPAEP GHVGRLHAVL DDCTPSTILT TTDSAEGVRK FIRGRFAKER TRVIAVDAVP NEVAATWQQP EANEETVAYL QYTSGSTRIP SGVQITHLNL PTNVLQVLNS LQGQEGDRGV SWLPFFHDMG LITVLLPSVL GHSFTFMTPA AFVRRPGRWI RELARKPEDT GGTFSAAPNF AFEHAAMRGL PRDDELPLDL SNVKGILNGS EPVSPASMRK FFEAFAPYGL KQTAVKPSYG LAEATLFVST TPMDESPTVI HVDRYELNNQ RFVEVPADAP NAVAQVSAGK VGVSEWAIIV DADTASELPD GQIGEIWLHG NNLGTGYWGK EEESAQTFNN ILKSRISESR AEGASDDASW VRTGDYGTYF KDHLYIAGRI KDLVIIDGRN HYPQDLEYTA QESTKALRVG YVAAFSVPAN QLAQVVLEDP HVGLKFDAED TSEQLVIVGE RAAGTHKLDY QPIVDDIRAA IAVRHGVAVR DVLLVPAGTI PRTSSGKIGR RACRAAYIDG SLRSGASSPT VFAAEF

Full NT Sequence (Expression Vector + Insert)

tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat attaacgctt acaatttagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga attaattctt agaaaaactc atcgagcatc aaatgaaact gcaatttatt catatcagga ttatcaatac catatttttg aaaaagccgt ttctgtaatg aaggagaaaa ctcaccgagg cagttccata ggatggcaag atcctggtat cggtctgcga ttccgactcg tccaacatca atacaaccta ttaatttccc ctcgtcaaaa ataaggttat caagtgagaa atcaccatga gtgacgactg aatccggtga gaatggcaaa agtttatgca tttctttcca gacttgttca acaggccagc cattacgctc gtcatcaaaa tcactcgcat caaccaaacc gttattcatt cgtgattgcg cctgagcgag acgaaatacg cgatcgctgt taaaaggaca attacaaaca ggaatcgaat gcaaccggcg caggaacact gccagcgcat caacaatatt ttcacctgaa tcaggatatt cttctaatac ctggaatgct gttttcccgg ggatcgcagt ggtgagtaac catgcatcat caggagtacg gataaaatgc ttgatggtcg gaagaggcat aaattccgtc agccagttta gtctgaccat ctcatctgta acatcattgg caacgctacc tttgccatgt ttcagaaaca actctggcgc atcgggcttc ccatacaatc gatagattgt cgcacctgat tgcccgacat tatcgcgagc ccatttatac ccatataaat cagcatccat gttggaattt aatcgcggcc tagagcaaga cgtttcccgt tgaatatggc tcataacacc ccttgtatta ctgtttatgt aagcagacag ttttattgtt catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcaa tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag ctgcggtaaa gctcatcagc gtggtcgtga agcgattcac agatgtctgc ctgttcatcc gcgtccagct cgttgagttt ctccagaagc gttaatgtct ggcttctgat aaagcgggcc atgttaaggg cggttttttc ctgtttggtc actgatgcct ccgtgtaagg gggatttctg ttcatggggg taatgatacc gatgaaacga gagaggatgc tcacgatacg ggttactgat gatgaacatg cccggttact ggaacgttgt gagggtaaac aactggcggt atggatgcgg cgggaccaga gaaaaatcac tcagggtcaa tgccagcgct tcgttaatac agatgtaggt gttccacagg gtagccagca gcatcctgcg atgcagatcc ggaacataat ggtgcagggc gctgacttcc gcgtttccag actttacgaa acacggaaac cgaagaccat tcatgttgtt gctcaggtcg cagacgtttt gcagcagcag tcgcttcacg ttcgctcgcg tatcggtgat tcattctgct aaccagtaag gcaaccccgc cagcctagcc gggtcctcaa cgacaggagc acgatcatgc gcacccgtgg ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gagatcccgg tgcctaatga gtgagctaac ttacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgccagggtg gtttttcttt tcaccagtga gacgggcaac agctgattgc ccttcaccgc ctggccctga gagagttgca gcaagcggtc cacgctggtt tgccccagca ggcgaaaatc ctgtttgatg gtggttaacg gcgggatata acatgagctg tcttcggtat cgtcgtatcc cactaccgag atatccgcac caacgcgcag cccggactcg gtaatggcgc gcattgcgcc cagcgccatc tgatcgttgg caaccagcat cgcagtggga acgatgccct cattcagcat ttgcatggtt tgttgaaaac cggacatggc actccagtcg ccttcccgtt ccgctatcgg ctgaatttga ttgcgagtga gatatttatg ccagccagcc agacgcagac gcgccgagac agaacttaat gggcccgcta acagcgcgat ttgctggtga cccaatgcga ccagatgctc cacgcccagt cgcgtaccgt cttcatggga gaaaataata ctgttgatgg gtgtctggtc agagacatca agaaataacg ccggaacatt agtgcaggca gcttccacag caatggcatc ctggtcatcc agcggatagt taatgatcag cccactgacg cgttgcgcga gaagattgtg caccgccgct ttacaggctt cgacgccgct tcgttctacc atcgacacca ccacgctggc acccagttga tcggcgcgag atttaatcgc cgcgacaatt tgcgacggcg cgtgcagggc cagactggag gtggcaacgc caatcagcaa cgactgtttg cccgccagtt gttgtgccac gcggttggga atgtaattca gctccgccat cgccgcttcc actttttccc gcgttttcgc agaaacgtgg ctggcctggt tcaccacgcg ggaaacggtc tgataagaga caccggcata ctctgcgaca tcgtataacg ttactggttt cacattcacc accctgaatt gactctcttc cgggcgctat catgccatac cgcgaaaggt tttgcgccat tcgatggtgt ccgggatctc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaatt aatacgactc actatagggg aattgtgagc ggataacaat tcccctctag aaataatttt gtttaacttt aagaaggaga tataccatgg ctcatcacca tcaccatcat atgggtaccc tggaagctca gacccagggt cctggttcga tgaccggaga gggtcgcatg gcataccaca acccgttcat agtgaatggt aagatcagat tcccaccaaa taccaacttg gttcggcacg tggaaaagtg ggcgaaggtt cgtggcgaca agcttgcata tcgattcctg gacttttcta cgggacgtga tggtgtggcg cgcgacattt tgtggtccga attctccgcg cgtaacagag cggtcggtgc ccgcctgcaa caagttaccc agccgggtga ccggatcgct atactttgtc cgcagaacct ggactactta atctctttct ttggagcgct gtattcaggc cgcattgcgg tgccgctgtt cgacccagca gagccgggtc atgtcggtcg ccttcacgcc gtgttggacg attgcactcc ttccacaatt cttaccacga ccgacagtgc cgaaggtgta agaaagttca tccgagggcg gtttgctaag gaacgaactc gcgtaatagc cgttgacgcg gtgccaaacg aggttgcagc cacctggcag cagccggaag caaacgagga aacagtggcc tatctgcagt acacgtccgg ctcgacccgg attccatccg gcgtgcagat aacccacctg aacctgccca ctaatgtttt gcaggtctta aatagcctgc aaggccagga aggtgaccgg ggcgtctctt ggttaccatt cttccacgac atgggtctga tcacagtact gttaccgtct gttctgggtc acagttttac ctttatgaca ccagctgctt tcgttcgtcg tcctggccgg tggattcgcg aactggccag aaagccggaa gacacaggcg ggaccttcag tgctgctccg aacttcgcat ttgaacacgc cgcgatgcgc ggacttcccc gggatgacga gttacccttg gatctttcca acgttaaagg catcctgaac gggagtgagc cggtgtcccc ggcatcaatg cggaagttct tcgaagcttt tgcgccgtac ggtctgaagc agaccgcggt taagccgagc tacgggctcg cggaagcgac gttgtttgtc agcactactc ccatggacga gtccccaact gtgatccacg tggaccgata cgagctgaac aatcagcgat tcgtagaggt gcctgctgat gccccaaacg cggtagctca ggtgtcagct ggcaaggtcg gagtctctga atgggccatc atcgtagatg ccgacacagc gtccgagctg ccggacggac agataggtga gatctggctt cacggcaaca acctcggtac tgggtattgg ggcaaagagg aagagtccgc acagacattc aacaacatcc ttaaaagccg tatatctgag tctcgagcgg aaggtgcctc ggacgacgcc agttgggtcc gtacgggcga ctacgggact tacttcaagg accatttata tatcgctggc cgcatcaagg acctggtcat catagatggt cggaaccatt acccccagga cctggaatac acggcccagg aaagcacgaa ggccttacgg gtgggctacg tagccgcctt ctccgtcccc gccaaccaac tcgcgcaggt tgttctggaa gacccgcacg tcggtctgaa gttcgatgct gaggacacaa gcgagcagtt ggtcatcgtc ggcgagcgtg ctgcaggtac tcacaagctt gactaccagc ccatagtcga cgacatacgg gctgcaatag cggttcgaca tggagtagcc gttcgggatg tactgctggt gccggcaggg acgataccac gcacatctag cggaaagatc ggccgtagag cctgccgtgc ggcgtacatt gacggttcac ttcgctcagg cgcctctagt ccgacggttt tcgctgccga attctgactc gagcaccacc accaccacca ctgagatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat