SSGCID: Target Page

MyleA.19462.a

Dephospho-CoA kinase CoaE; uncharacterized protein

CENTER ID:	MyleA.19462.a
ORGANISM:	Mycobacterium leprae Br4923
ASSOCIATED DISEASE:	Hansen's disease, leprosy
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MyleA.19462.a.B1.GE41030	full length		1	410
MyleA.19462.a.B2.GE41031	nterm domain		1	204

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
UniProt:	A0A0H3MQU4

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MLCIGITGGI GAGKSLLSST FSQCGGIVVD GDVLAREVVQ PGTKGLSSLV DAFGQDILLP GGALDRRALA VKAFRDDAAR NVLNGIVHPL VANRRAEIIA AISEDAVVVE DIPLLVESGM AHLFPLVVVV HADVELRVRR LVEQRGVAET DARARIAAQA SDEERRAVAD VWLDNSSTPE VLVQRARDLW YHRILPFAYN LSQRQAVYAP AGLVTSDPIW LGQAKRIVAR LKTTCGHKAL RVDHIGSTAV PHYPGFPDFQ AKDIIDIQIT VESLAMADEL ADPLLSAGYP RLEHVTGDAA KTNARSTVDR YEHSSDPNLW HKRFHASADP GRPTYVHIRV AGWPNQQFGL LFVDWLKANP GVRADYLDVK RTADRLAAGD MGRYADAKEP WLLDAYRRAW EWADSTGWRL

NT Sequence

atgctgtgca tcgggatcac cggtggcatc ggcgccggga agtctttgct gtctagtacg ttctcacagt gtggtgggat cgtcgtcgac ggcgatgtgc tggcacgcga agtggtgcag cccggcacca aaggattgtc atcgctggtc gatgcatttg gtcaggacat cctgcttcct ggcggtgcgt tggatcggcg ggctttggct gtcaaggctt ttcgagacga cgcggcgcgc aatgtgctta acgggattgt gcacccactg gtggccaacc gccgcgcgga gatcattgcg gcgatatccg aggacgcggt tgtggtcgaa gacattcctc tgctggtgga atccgggatg gcgcacctgt tcccgctggt cgttgtggtg catgccgacg tcgaactgcg agtacgcagg ctggttgagc aacgtggtgt ggccgaaacg gatgcgcgtg ccaggatcgc tgcacaggct agcgatgagg agcgtcgtgc tgttgctgat gtctggttag acaattcaag tacaccagag gttttggtcc aacgggctcg tgacctatgg taccatcgca tattgccgtt cgcctacaac ctgagtcagc gtcaggcggt ctatgcaccg gcaggtttgg tcacgtctga tccgatctgg ctggggcagg cgaagcgcat tgttgctcgg ctgaagacca cgtgtggcca taaggcgtta cgcgttgacc acatcggctc gaccgcggtg ccgcattatc caggttttcc tgattttcag gccaaagaca tcatcgacat tcagatcacc gtcgagtcac tagcgatggc tgacgaactc gccgaccctc tgttgtctgc cgggtacccg cgcctggagc acgtcaccgg tgatgccgcc aagaccaatg cccgcagcac cgtagatcgc tacgaacaca gtagcgaccc aaatttatgg cacaagaggt ttcatgcctc tgcagacccc ggtcgaccga catacgtgca tatccgggtg gctggctggc cgaatcagca attcggtttg ctgttcgtcg actggttgaa agctaacccg ggtgtgcgtg ccgactactt ggacgtcaaa cgcacggcgg accggctggc ggccggagac atgggccgtt acgccgacgc gaaggaacca tggctcctcg acgcctaccg gcgagcgtgg gagtgggccg actccacggg ttggcggctc tag

Details for MyleA.19462.a.B1.GE41030

HARVESTED ON:	1/4/2017
SEQUENCED ON:	1/5/2017
EXPECTED MW:	46kDa
OBSERVED MW:	46kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Many (50-100)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	100
PERCENT COVERAGE:	98

Validated AA Sequence

MAHHHHHHML CIGITGGIGA GKSLLSSTFS QCGGIVVDGD VLAREVVQPG TKGLSSLVDA FGQDILLPGG ALDRRALAVK AFRDDAARNV LNGIVHPLVA NRRAEIIAAI SEDAVVVEDI PLLVESGMAH LFPLVVVVHA DVELRVRRLV EQRGVAETDA RARIAAQASD EERRAVADVW LDNSSTPEVL VQRARDLWYH RILPFAYNLS QRQAVYAPAG LVTSDPIWLG QAKRIVARLK TTCGHKALRV DHIGSTAVPH YPGFPDFQAK DIIDIQITVE SLAMADELAD PLLSAGYPRL EHVTGDAAKT NARSTVDRYE HSSDPNLWHK RFHASADPGR PTYVHIRVAG WPNQQFGLLF VDWLKANPGV RADYLDVKRT ADRLAAGDMG RYADAKEPWL LDAYRRAWEW

Validated NT Sequence

gcccactccc acgctcgccg gtaggcgtcg aggagccatg gttccttcgc gtcggcgtaa cggcccatgt ctccggccgc cagccggtcc gccgtgcgtt tgacgtccaa gtagtcggca cgcacacccg ggttagcttt caaccagtcg acgaacagca aaccgaattg ctgattcggc cagccagcca cccggatatg cacgtatgtc ggtcgaccgg ggtctgcaga ggcatgaaac ctcttgtgcc ataaatttgg gtcgctactg tgttcgtagc gatctacggt gctgcgggca ttggtcttgg cggcatcacc ggtgacgtgc tccaggcgcg ggtacccggc agacaacaga gggtcggcga gttcgtcagc catcgctagt gactcgacgg tgatctgaat gtcgatgatg tctttggcct gaaaatcagg aaaacctgga taatgcggca ccgcggtcga gccgatgtgg tcaacgcgta acgccttatg gccacacgtg gtcttcagcc gagcaacaat gcgcttcgcc tgccccagcc agatcggatc agacgtgacc aaacctgccg gtgcatagac cgcctgacgc tgactcaggt tgtaggcgaa cggcaatatg cgatggtacc ataggtcacg agcccgttgg accaaaacct ctggtgtact tgaattgtct aaccagacat cagcaacagc acgacgctcc tcatcgctag cctgtgcagc gatcctggca cgcgcatccg tttcggccac accacgttgc tcaaccagcc tgcgtactcg cagttcgacg tcggcatgca ccacaacgac cagcgggaac aggtgcgcca tcccggattc caccagcaga ggaatgtctt cgaccacaac cgcgtcctcg gatatcgccg caatgatctc cgcgcggcgg ttggccacca gtgggtgcac aatcccgtta agcacattgc gcgccgcgtc gtctcgaaaa gccttgacag ccaaagcccg ccgatccaac gcaccgccag gaagcaggat gtcctgacca aatgcatcga ccagcgatga caatcctttg gtgccgggct gcaccacttc gcgtgccagc acatcgccgt cgacgacgat cccaccacac tgtgagaacg tactagacag caaagacttc ccggcgccga tgccaccggt gatcccgatg cacagcatat ggtggtggtg gtggtgagcc at

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgctgt gcatcgggat caccggtggc atcggcgccg ggaagtcttt gctgtctagt acgttctcac agtgtggtgg gatcgtcgtc gacggcgatg tgctggcacg cgaagtggtg cagcccggca ccaaaggatt gtcatcgctg gtcgatgcat ttggtcagga catcctgctt cctggcggtg cgttggatcg gcgggctttg gctgtcaagg cttttcgaga cgacgcggcg cgcaatgtgc ttaacgggat tgtgcaccca ctggtggcca accgccgcgc ggagatcatt gcggcgatat ccgaggacgc ggttgtggtc gaagacattc ctctgctggt ggaatccggg atggcgcacc tgttcccgct ggtcgttgtg gtgcatgccg acgtcgaact gcgagtacgc aggctggttg agcaacgtgg tgtggccgaa acggatgcgc gtgccaggat cgctgcacag gctagcgatg aggagcgtcg tgctgttgct gatgtctggt tagacaattc aagtacacca gaggttttgg tccaacgggc tcgtgaccta tggtaccatc gcatattgcc gttcgcctac aacctgagtc agcgtcaggc ggtctatgca ccggcaggtt tggtcacgtc tgatccgatc tggctggggc aggcgaagcg cattgttgct cggctgaaga ccacgtgtgg ccataaggcg ttacgcgttg accacatcgg ctcgaccgcg gtgccgcatt atccaggttt tcctgatttt caggccaaag acatcatcga cattcagatc accgtcgagt cactagcgat ggctgacgaa ctcgccgacc ctctgttgtc tgccgggtac ccgcgcctgg agcacgtcac cggtgatgcc gccaagacca atgcccgcag caccgtagat cgctacgaac acagtagcga cccaaattta tggcacaaga ggtttcatgc ctctgcagac cccggtcgac cgacatacgt gcatatccgg gtggctggct ggccgaatca gcaattcggt ttgctgttcg tcgactggtt gaaagctaac ccgggtgtgc gtgccgacta cttggacgtc aaacgcacgg cggaccggct ggcggccgga gacatgggcc gttacgccga cgcgaaggaa ccatggctcc tcgacgccta ccggcgagcg tgggagtggg ccgactccac gggttggcgg ctctgagtaa gataggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaat tcttgaagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt ccagctcgtt gagtttctcc agaagcgtta atgtctggct tctgataaag cgggccatgt taagggcggt tttttcctgt ttggtcactg atgcctccgt gtaaggggga tttctgttca tgggggtaat gataccgatg aaacgagaga ggatgctcac gatacgggtt actgatgatg aacatgcccg gttactggaa cgttgtgagg gtaaacaact ggcggtatgg atgcggcggg accagagaaa aatcactcag ggtcaatgcc agcgcttcgt taatacagat gtaggtgttc cacagggtag ccagcagcat cctgcgatgc agatccggaa cataatggtg cagggcgctg acttccgcgt ttccagactt tacgaaacac ggaaaccgaa gaccattcat gttgttgctc aggtcgcaga cgttttgcag cagcagtcgc ttcacgttcg ctcgcgtatc ggtgattcat tctgctaacc agtaaggcaa ccccgccagc ctagccgggt cctcaacgac aggagcacga tcatgcgcac ccgtggccag gacccaacgc tgcccgagat gcgccgcgtg cggctgctgg agatggcgga cgcgatggat atgttctgcc aagggttggt ttgcgcattc acagttctcc gcaagaattg attggctcca attcttggag tggtgaatcc gttagcgagg tgccgccggc ttccattcag gtcgaggtgg cccggctcca tgcaccgcga cgcaacgcgg ggaggcagac aaggtatagg gcggcgccta caatccatgc caacccgttc catgtgctcg ccgaggcggc ataaatcgcc gtgacgatca gcggtccagt gatcgaagtt aggctggtaa gagccgcgag cgatccttga agctgtccct gatggtcgtc atctacctgc ctggacagca tggcctgcaa cgcgggcatc ccgatgccgc cggaagcgag aagaatcata atggggaagg ccatccagcc tcgcgtcgcg aacgccagca agacgtagcc cagcgcgtcg gccgccatgc cggcgataat ggcctgcttc tcgccgaaac gtttggtggc gggaccagtg acgaaggctt gagcgagggc gtgcaagatt ccgaataccg caagcgacag gccgatcatc gtcgcgctcc agcgaaagcg gtcctcgccg aaaatgaccc agagcgctgc cggcacctgt cctacgagtt gcatgataaa gaagacagtc ataagtgcgg cgacgatagt catgccccgc gcccaccgga aggagctgac tgggttgaag gctctcaagg gcatcggtcg acgctctccc ttatgcgact cctgcattag gaagcagccc agtagtaggt tgaggccgtt gagcaccgcc gccgcaagga atggtgcatg caaggagatg gcgcccaaca gtcccccggc cacggggcct gccaccatac ccacgccgaa acaagcgctc atgagcccga agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta gaggatcgag atctcgatcc cgcgaaat

Details for MyleA.19462.a.B2.GE41031

HARVESTED ON:	1/4/2017
SEQUENCED ON:	1/5/2017
EXPECTED MW:	23kDa
OBSERVED MW:	23kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	None
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

Validated NT Sequence

atcttactca cttaacgctg actcaggttg taggcgaacg gcaatatgcg atggtaccat aggtcacgag cccgttggac caaaacctct ggtgtacttg aattgtctaa ccagacatca gcaacagcac gacgctcctc atcgctagcc tgtgcagcga tcctggcacg cgcatccgtt tcggccacac cacgttgctc aaccagcctg cgtactcgca gttcgacgtc ggcatgcacc acaacgacca gcgggaacag gtgcgccatc ccggattcca ccagcagagg aatgtcttcg accacaaccg cgtcctcgga tatcgccgca atgatctccg cgcggcggtt ggccaccagt gggtgcacaa tcccgttaag cacattgcgc gccgcgtcgt ctcgaaaagc cttgacagcc aaagcccgcc gatccaacgc accgccagga agcaggatgt cctgaccaaa tgcatcgacc agcgatgaca atcctttggt gccgggctgc accacttcgc gtgccagcac atcgccgtcg acgacgatcc caccacactg tgagaacgta ctagacagca aagacttccc ggcgccgatg ccaccggtga tcccgatgca cagcatatgg tggtggtggt ggtgagccat

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgctgt gcatcgggat caccggtggc atcggcgccg ggaagtcttt gctgtctagt acgttctcac agtgtggtgg gatcgtcgtc gacggcgatg tgctggcacg cgaagtggtg cagcccggca ccaaaggatt gtcatcgctg gtcgatgcat ttggtcagga catcctgctt cctggcggtg cgttggatcg gcgggctttg gctgtcaagg cttttcgaga cgacgcggcg cgcaatgtgc ttaacgggat tgtgcaccca ctggtggcca accgccgcgc ggagatcatt gcggcgatat ccgaggacgc ggttgtggtc gaagacattc ctctgctggt ggaatccggg atggcgcacc tgttcccgct ggtcgttgtg gtgcatgccg acgtcgaact gcgagtacgc aggctggttg agcaacgtgg tgtggccgaa acggatgcgc gtgccaggat cgctgcacag gctagcgatg aggagcgtcg tgctgttgct gatgtctggt tagacaattc aagtacacca gaggttttgg tccaacgggc tcgtgaccta tggtaccatc gcatattgcc gttcgcctac aacctgagtc agcgttgagt aagataggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagcgcttc gttaatacag atgtaggtgt tccacagggt agccagcagc atcctgcgat gcagatccgg aacataatgg tgcagggcgc tgacttccgc gtttccagac tttacgaaac acggaaaccg aagaccattc atgttgttgc tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc attctgctaa ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg acaggagcac gatcatgcgc acccgtggcc aggacccaac gctgcccgag atgcgccgcg tgcggctgct ggagatggcg gacgcgatgg atatgttctg ccaagggttg gtttgcgcat tcacagttct ccgcaagaat tgattggctc caattcttgg agtggtgaat ccgttagcga ggtgccgccg gcttccattc aggtcgaggt ggcccggctc catgcaccgc gacgcaacgc ggggaggcag acaaggtata gggcggcgcc tacaatccat gccaacccgt tccatgtgct cgccgaggcg gcataaatcg ccgtgacgat cagcggtcca gtgatcgaag ttaggctggt aagagccgcg agcgatcctt gaagctgtcc ctgatggtcg tcatctacct gcctggacag catggcctgc aacgcgggca tcccgatgcc gccggaagcg agaagaatca taatggggaa ggccatccag cctcgcgtcg cgaacgccag caagacgtag cccagcgcgt cggccgccat gccggcgata atggcctgct tctcgccgaa acgtttggtg gcgggaccag tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac cgcaagcgac aggccgatca tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac ccagagcgct gccggcacct gtcctacgag ttgcatgata aagaagacag tcataagtgc ggcgacgata gtcatgcccc gcgcccaccg gaaggagctg actgggttga aggctctcaa gggcatcggt cgacgctctc ccttatgcga ctcctgcatt aggaagcagc ccagtagtag gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca tgcaaggaga tggcgcccaa cagtcccccg gccacggggc ctgccaccat acccacgccg aaacaagcgc tcatgagccc gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc cagcaaccgc acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagaggatcg agatctcgat cccgcgaaat