SSGCID: Target Page

MyleA.19288.a

Polyphosphate kinase (EC 2.7.4.1) (ATP-polyphosphate phosphotransferase) (Polyphosphoric acid kinase)

CENTER ID:	MyleA.19288.a
ORGANISM:	Mycobacterium leprae Br4923
ASSOCIATED DISEASE:	Hansen's disease, leprosy
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MyleA.19288.a.B2.GE40597	ordered domain		47	739

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|561304.4.peg.3184
UniProt:	B8ZS08

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MMSNDLLVTD IEAEARTEEN IWHSDNSALA APPAATTSAS QDQLPDDRYL NRESSWLDFN ARVLALAADN SLPLLERAKF LAIFASNLDE FYMVRVAGLK RRDEMDLSVR SADGLTPREQ LSRIGEQTQW IASRHARVFL DSVLPALGEE GIYIVTWTDL DQAERDQLST YFNEQVFPVL TPLAVDPAHP FPFVSGLSLN LAVTVKQPED GTQHFARVKV PNNVDRFVEL ANRGAVRDTS AGDEGQLIHR FLPMEELIAA FLPVLFPGTE IVEHHAFRIT RNADFEVEED RDEDLLQALE RELARRRFGS PVRLEIADDM TESMLELLLR ELDVHPSDVI EVPGLLDLSS LWQIYGLDRP ALKDRAFIPD THPAFAERET PKSIFATLRE GDVLVHHPYH SFATSVQRFI EQATADPDVL AIKQTLYRTS GDSPIVLALI EAAEAGKQVV ALVEIKARFD EQANIRWARA LEHAGVHVVY GIVGLKTHCK TCLVVRREGP TIRRYCHIGT GNYNSKTARL YEDVGLLTAA PDIGADLTDL FNSLTGYSRK LAYRNLLVAP YGIRRGIIER VEREVAAHRE SGPQTGKGLI RLKMNALVDE QVIDALYRAS QAGVRVEVVV RGICALRPGT EGFSENIFVR SILGRFLEHS RIIHFRAIDE FWIGSADMMH RNLDRRVEVL AQVKDSRLTA QLDELLKSAL DPFTRCWELR PDGQWTASPQ KGQQVRDHQE SLMERHRSR

NT Sequence

atgatgagca atgatctttt ggtgaccgac atcgaagctg aggcccgtac cgaagagaat atatggcatt cagacaactc cgccctcgca gcaccgcccg cggcgaccac ctctgcgagt caggaccagc tgcccgacga ccgctacctc aaccgggaat caagttggct ggatttcaat gcgcgtgtgc tcgcactagc cgccgataac tcgttgcctt tgttggagcg agccaagttt ctggctatct tcgcatccaa tctcgacgag ttctacatgg ttcgagtcgc cggcctcaag cgccgcgacg agatggactt gtcggtgcgc tctgctgacg gtctcacccc acgtgagcaa ctgagccgca taggcgagca gacccaatgg atcgccagcc ggcacgcgcg ggtgttcctc gattcggtgt tacccgcact cggtgaggag ggtatctaca ttgtcacgtg gacggattta gatcaggccg aacgtgacca gttatcgacc tatttcaacg agcaggtctt ccccgtcctg acaccactgg ctgtcgatcc cgcccacccg tttccgtttg tcagcgggtt aagtctgaac ctggctgtca ccgtcaagca acccgaagac ggtacccagc atttcgcccg ggtcaaagtg cctaacaacg tcgatcgatt cgtagaactc gctaaccgcg gggcggtcag agatacctct gccggagatg aaggtcagct cattcaccgg ttcctgccca tggaggagct gatcgcggca ttccttccgg tgctcttccc tggcacggaa atcgtcgagc atcatgcatt ccgcattacc cgcaacgctg acttcgaggt tgaagaagac cgtgacgaag acctactaca ggcgctggaa cgagaattgg cgcgtcgacg gttcggttcg ccggtgcgac tcgagatcgc cgacgacatg accgagagca tgctggaact gctgttgcgg gaactcgacg tgcatcccag cgacgtcatc gaagtgcccg ggctgcttga cctgtcgtcg ttgtggcaaa tttacgggct ggaccgaccc gcactcaaag accgcgcatt cattcccgac actcatcccg ctttcgccga acgggaaact cccaagagca tcttcgcgac gctgcgcgaa ggcgatgttc tggtgcacca tccctatcac tcgtttgcca ccagcgtgca gagattcatt gaacaggcca ccgccgaccc cgatgtgctg gcgatcaaac aaacgctgta ccgcacctcc ggtgattcac cgatcgtgct ggcgctcatc gaagctgccg aggcaggaaa gcaagtggtg gcactggtcg aaatcaaggc tcgctttgac gagcaggcca acatccggtg ggcgcgcgcg ctggaacacg caggcgtgca tgtggtgtac gggatcgttg ggctcaagac acactgcaag acctgcctcg tggtgcgccg tgagggcccg acgatccggc ggtactgcca tatcggaacc ggcaactaca acagcaagac cgctcgactg tacgaagatg tcggtctgtt gaccgcagca cccgatatcg gcgccgactt gactgacttg ttcaactcgc ttaccggcta ctcgcgtaag ctggcttacc gcaaccttct ggtggcaccg tatggaattc gccgaggcat tatcgagcgg gtcgagcgcg aggtggccgc ccatcgcgaa tccggccccc aaaccggtaa gggcctgatc cgactcaaga tgaacgctct ggtcgacgaa caagttatcg atgcgctgta tcgcgcgtca caggcaggcg tgcgggtcga ggtggtggtg cgcggcatct gcgcactacg cccaggcacc gaaggttttt cggaaaacat tttcgtccgc tcgattctcg gccgtttcct cgagcattca cggatcatcc atttccgagc catcgatgag ttctggatcg gaagcgccga catgatgcac cgcaacctag atcgacgagt cgaggtgctg gcgcaagtta aagactcgag gctgaccgcg cagctagatg agttgttgaa gtccgcgctg gacccgttca cccggtgttg ggaactgcga cccgacgggc agtggaccgc ttcgccgcag aaaggccagc aggtgcgcga ccaccaagaa tcgttgatgg aacggcaccg cagccgctga

Details for MyleA.19288.a.B2.GE40597

HARVESTED ON:	10/18/2016
SEQUENCED ON:	10/19/2016
EXPECTED MW:	79kDa
OBSERVED MW:	79kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	data unavailable
PERCENT COVERAGE:	data unavailable

Validated AA Sequence

sequence unavailable

Validated NT Sequence

sequence unavailable

Expected Protein Sequence

MAHHHHHHDR YLNRESSWLD FNARVLALAA DNSLPLLERA KFLAIFASNL DEFYMVRVAG LKRRDEMDLS VRSADGLTPR EQLSRIGEQT QWIASRHARV FLDSVLPALG EEGIYIVTWT DLDQAERDQL STYFNEQVFP VLTPLAVDPA HPFPFVSGLS LNLAVTVKQP EDGTQHFARV KVPNNVDRFV ELANRGAVRD TSAGDEGQLI HRFLPMEELI AAFLPVLFPG TEIVEHHAFR ITRNADFEVE EDRDEDLLQA LERELARRRF GSPVRLEIAD DMTESMLELL LRELDVHPSD VIEVPGLLDL SSLWQIYGLD RPALKDRAFI PDTHPAFAER ETPKSIFATL REGDVLVHHP YHSFATSVQR FIEQATADPD VLAIKQTLYR TSGDSPIVLA LIEAAEAGKQ VVALVEIKAR FDEQANIRWA RALEHAGVHV VYGIVGLKTH CKTCLVVRRE GPTIRRYCHI GTGNYNSKTA RLYEDVGLLT AAPDIGADLT DLFNSLTGYS RKLAYRNLLV APYGIRRGII ERVEREVAAH RESGPQTGKG LIRLKMNALV DEQVIDALYR ASQAGVRVEV VVRGICALRP GTEGFSENIF VRSILGRFLE HSRIIHFRAI DEFWIGSADM MHRNLDRRVE VLAQVKDSRL TAQLDELLKS ALDPFTRCWE LRPDGQWTAS PQKGQQVRDH QESLMERHRS R

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgaccgct acctcaaccg ggaatcaagt tggctggatt tcaatgcgcg tgtgctcgca ctagccgccg ataactcgtt gcctttgttg gagcgagcca agtttctggc tatcttcgca tccaatctcg acgagttcta catggttcga gtcgccggcc tcaagcgccg cgacgagatg gacttgtcgg tgcgctctgc tgacggtctc accccacgtg agcaactgag ccgcataggc gagcagaccc aatggatcgc cagccggcac gcgcgggtgt tcctcgattc ggtgttaccc gcactcggtg aggagggtat ctacattgtc acgtggacgg atttagatca ggccgaacgt gaccagttat cgacctattt caacgagcag gtcttccccg tcctgacacc actggctgtc gatcccgccc acccgtttcc gtttgtcagc gggttaagtc tgaacctggc tgtcaccgtc aagcaacccg aagacggtac ccagcatttc gcccgggtca aagtgcctaa caacgtcgat cgattcgtag aactcgctaa ccgcggggcg gtcagagata cctctgccgg agatgaaggt cagctcattc accggttcct gcccatggag gagctgatcg cggcattcct tccggtgctc ttccctggca cggaaatcgt cgagcatcat gcattccgca ttacccgcaa cgctgacttc gaggttgaag aagaccgtga cgaagaccta ctacaggcgc tggaacgaga attggcgcgt cgacggttcg gttcgccggt gcgactcgag atcgccgacg acatgaccga gagcatgctg gaactgctgt tgcgggaact cgacgtgcat cccagcgacg tcatcgaagt gcccgggctg cttgacctgt cgtcgttgtg gcaaatttac gggctggacc gacccgcact caaagaccgc gcattcattc ccgacactca tcccgctttc gccgaacggg aaactcccaa gagcatcttc gcgacgctgc gcgaaggcga tgttctggtg caccatccct atcactcgtt tgccaccagc gtgcagagat tcattgaaca ggccaccgcc gaccccgatg tgctggcgat caaacaaacg ctgtaccgca cctccggtga ttcaccgatc gtgctggcgc tcatcgaagc tgccgaggca ggaaagcaag tggtggcact ggtcgaaatc aaggctcgct ttgacgagca ggccaacatc cggtgggcgc gcgcgctgga acacgcaggc gtgcatgtgg tgtacgggat cgttgggctc aagacacact gcaagacctg cctcgtggtg cgccgtgagg gcccgacgat ccggcggtac tgccatatcg gaaccggcaa ctacaacagc aagaccgctc gactgtacga agatgtcggt ctgttgaccg cagcacccga tatcggcgcc gacttgactg acttgttcaa ctcgcttacc ggctactcgc gtaagctggc ttaccgcaac cttctggtgg caccgtatgg aattcgccga ggcattatcg agcgggtcga gcgcgaggtg gccgcccatc gcgaatccgg cccccaaacc ggtaagggcc tgatccgact caagatgaac gctctggtcg acgaacaagt tatcgatgcg ctgtatcgcg cgtcacaggc aggcgtgcgg gtcgaggtgg tggtgcgcgg catctgcgca ctacgcccag gcaccgaagg tttttcggaa aacattttcg tccgctcgat tctcggccgt ttcctcgagc attcacggat catccatttc cgagccatcg atgagttctg gatcggaagc gccgacatga tgcaccgcaa cctagatcga cgagtcgagg tgctggcgca agttaaagac tcgaggctga ccgcgcagct agatgagttg ttgaagtccg cgctggaccc gttcacccgg tgttgggaac tgcgacccga cgggcagtgg accgcttcgc cgcagaaagg ccagcaggtg cgcgaccacc aagaatcgtt gatggaacgg caccgcagcc gctgagtaag ataggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaatt cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg cagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca tcagcgtggt cgtgaagcga ttcacagatg tctgcctgtt catccgcgtc cagctcgttg agtttctcca gaagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt tggtcactga tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa atcactcagg gtcaatgcca gcgcttcgtt aatacagatg taggtgttcc acagggtagc cagcagcatc ctgcgatgca gatccggaac ataatggtgc agggcgctga cttccgcgtt tccagacttt acgaaacacg gaaaccgaag accattcatg ttgttgctca ggtcgcagac gttttgcagc agcagtcgct tcacgttcgc tcgcgtatcg gtgattcatt ctgctaacca gtaaggcaac cccgccagcc tagccgggtc ctcaacgaca ggagcacgat catgcgcacc cgtggccagg acccaacgct gcccgagatg cgccgcgtgc ggctgctgga gatggcggac gcgatggata tgttctgcca agggttggtt tgcgcattca cagttctccg caagaattga ttggctccaa ttcttggagt ggtgaatccg ttagcgaggt gccgccggct tccattcagg tcgaggtggc ccggctccat gcaccgcgac gcaacgcggg gaggcagaca aggtataggg cggcgcctac aatccatgcc aacccgttcc atgtgctcgc cgaggcggca taaatcgccg tgacgatcag cggtccagtg atcgaagtta ggctggtaag agccgcgagc gatccttgaa gctgtccctg atggtcgtca tctacctgcc tggacagcat ggcctgcaac gcgggcatcc cgatgccgcc ggaagcgaga agaatcataa tggggaaggc catccagcct cgcgtcgcga acgccagcaa gacgtagccc agcgcgtcgg ccgccatgcc ggcgataatg gcctgcttct cgccgaaacg tttggtggcg ggaccagtga cgaaggcttg agcgagggcg tgcaagattc cgaataccgc aagcgacagg ccgatcatcg tcgcgctcca gcgaaagcgg tcctcgccga aaatgaccca gagcgctgcc ggcacctgtc ctacgagttg catgataaag aagacagtca taagtgcggc gacgatagtc atgccccgcg cccaccggaa ggagctgact gggttgaagg ctctcaaggg catcggtcga cgctctccct tatgcgactc ctgcattagg aagcagccca gtagtaggtt gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgc aaggagatgg cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa caagcgctca tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata taggcgccag caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag aggatcgaga tctcgatccc gcgaaat