SSGCID: Target Page

MyleA.19281.a

Putative Rieske iron-sulphur reductase component

CENTER ID:	MyleA.19281.a
ORGANISM:	Mycobacterium leprae Br4923
ASSOCIATED DISEASE:	Hansen's disease, leprosy
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MyleA.19281.a.B2.GE40596	Rieske iron-sulfur domain		207	394

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
UniProt:	A0A0H3MUM7

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MSLNPKDGAG ASRELDETTQ GTMSREELVA LGGRLDGVNT VYKEQRWPIE GTKAEKRTER MVTFWLLLGG VLGLTLLLIF LFWPWEYKPK EASGSFLYTI ATPLYGLTFG LSILAIAIGA VQFQKRFIPE EISIQERHDG ASPEIDRKTV VANLTDALES STIRRRKLIG LSLGVGLGAF GLSTLVTFIG GFIKNPWKPV VPTANGMKAM LWTSGWTPRY HGETIYLARA TGSHSGAPFI RMRPEDVDAG GMETVFPWRE SDGDGTTMES HEKLITIKMG IRNPVMLIRI RPSDMPRVVK RRGQENFNFG EFFAFTKVCS HLGCPSSLYE QQSYRILCPC HQSQFDALQY AKPIFGPAAR ALAQLPITID TDGYLVANGD FVEPVGPAFW ERTT

NT Sequence

atgagcctta acccgaaaga cggcgccggc gcttcccggg agctcgacga gacaacgcag gggacgatgt cacgcgagga actggtagcc ctgggcggtc ggctcgatgg tgttaacacc gtctacaagg agcagcgttg gccgatcgag ggcaccaagg cggaaaagcg caccgaacga atggtaacct tctggctatt gctgggtggc gtattgggtc tgacgttact actgatcttc ctgttttggc cgtgggagta caaacccaaa gaggcctcag gcagtttcct ctacaccatt gccaccccgc tgtatggtct gaccttcggg ctttccatcc tggcgatcgc tattggcgcg gtgcagtttc agaagagatt catcccggaa gagatttcga tccaggaacg ccatgacggt gcatcgcccg agatcgaccg caagacagtc gtagcgaacc taaccgacgc tcttgagagc tcaacgatca ggcgccgcaa gctaatcggg ctgtcgttgg gcgtgggctt gggcgctttt ggcctcagca cgttggtgac gtttattggc ggtttcatca aaaacccatg gaagccagtc gtacccaccg cgaatgggat gaaggccatg ctttggactt ccggctggac cccacgatac catggggaga caatctatct agcgagagct accggctcgc actcgggagc accgtttatc cggatgcgtc cggaagacgt cgacgcaggc gggatggaga ccgtctttcc gtggcgcgag tccgacggcg acggcaccac catggaatca cacgaaaaac tgataacgat caagatgggt atccgcaacc cggtaatgct catccgtatc cggcccagtg atatgcctcg tgtggtcaaa cgccgaggcc aggagaactt caacttcggc gagttcttcg ccttcaccaa ggtctgctcg catttgggtt gcccatcctc actatacgaa cagcagtcct accgaatcct gtgcccttgt caccaatctc agttcgacgc gctgcagtat gccaagccga tattcggtcc ggccgcgcgc gcgctggcac aactacccat caccatcgac accgacgggt atctggttgc caacggtgac tttgtcgagc cggtcgggcc agcattctgg gagcgcacaa catga

Details for MyleA.19281.a.B2.GE40596

HARVESTED ON:	10/18/2016
SEQUENCED ON:	10/19/2016
EXPECTED MW:	22kDa
OBSERVED MW:	22kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMK AMLWTSGWTP RYHGETIYLA RATGSHSGAP FIRMRPEDVD AGGMETVFPW RESDGDGTTM ESHEKLITIK MGIRNPVMLI RIRPSDMPRV VKRRGQENFN FGEFFAFTKV CSHLGCPSSL YEQQSYRILC PCHQSQFDAL QYAKPIFGPA ARALAQLPIT IDTDGYLVAN GDFVEPVGPA FWERTT

Validated NT Sequence

atcttactca cttatgttgt gcgctcccag aatgctggcc cgaccggctc gacaaagtca ccgttggcaa ccagataccc gtcggtgtcg atggtgatgg gtagttgtgc cagcgcgcgc gcggccggac cgaatatcgg cttggcatac tgcagcgcgt cgaactgaga ttggtgacaa gggcacagga ttcggtagga ctgctgttcg tatagtgagg atgggcaacc caaatgcgag cagaccttgg tgaaggcgaa gaactcgccg aagttgaagt tctcctggcc tcggcgtttg accacacgag gcatatcact gggccggata cggatgagca ttaccgggtt gcggataccc atcttgatcg ttatcagttt ttcgtgtgat tccatggtgg tgccgtcgcc gtcggactcg cgccacggaa agacggtctc catcccgcct gcgtcgacgt cttccggacg catccggata aacggtgctc ccgagtgcga gccggtagct ctcgctagat agattgtctc cccatggtat cgtggggtcc agccggaagt ccaaagcatg gccttcatat ggtggtggtg gtggtgagcc at

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaagg ccatgctttg gacttccggc tggaccccac gataccatgg ggagacaatc tatctagcga gagctaccgg ctcgcactcg ggagcaccgt ttatccggat gcgtccggaa gacgtcgacg caggcgggat ggagaccgtc tttccgtggc gcgagtccga cggcgacggc accaccatgg aatcacacga aaaactgata acgatcaaga tgggtatccg caacccggta atgctcatcc gtatccggcc cagtgatatg cctcgtgtgg tcaaacgccg aggccaggag aacttcaact tcggcgagtt cttcgccttc accaaggtct gctcgcattt gggttgccca tcctcactat acgaacagca gtcctaccga atcctgtgcc cttgtcacca atctcagttc gacgcgctgc agtatgccaa gccgatattc ggtccggccg cgcgcgcgct ggcacaacta cccatcacca tcgacaccga cgggtatctg gttgccaacg gtgactttgt cgagccggtc gggccagcat tctgggagcg cacaacatga gtaagatagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaattcttga agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgcagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatata tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag ctgcggtaaa gctcatcagc gtggtcgtga agcgattcac agatgtctgc ctgttcatcc gcgtccagct cgttgagttt ctccagaagc gttaatgtct ggcttctgat aaagcgggcc atgttaaggg cggttttttc ctgtttggtc actgatgcct ccgtgtaagg gggatttctg ttcatggggg taatgatacc gatgaaacga gagaggatgc tcacgatacg ggttactgat gatgaacatg cccggttact ggaacgttgt gagggtaaac aactggcggt atggatgcgg cgggaccaga gaaaaatcac tcagggtcaa tgccagcgct tcgttaatac agatgtaggt gttccacagg gtagccagca gcatcctgcg atgcagatcc ggaacataat ggtgcagggc gctgacttcc gcgtttccag actttacgaa acacggaaac cgaagaccat tcatgttgtt gctcaggtcg cagacgtttt gcagcagcag tcgcttcacg ttcgctcgcg tatcggtgat tcattctgct aaccagtaag gcaaccccgc cagcctagcc gggtcctcaa cgacaggagc acgatcatgc gcacccgtgg ccaggaccca acgctgcccg agatgcgccg cgtgcggctg ctggagatgg cggacgcgat ggatatgttc tgccaagggt tggtttgcgc attcacagtt ctccgcaaga attgattggc tccaattctt ggagtggtga atccgttagc gaggtgccgc cggcttccat tcaggtcgag gtggcccggc tccatgcacc gcgacgcaac gcggggaggc agacaaggta tagggcggcg cctacaatcc atgccaaccc gttccatgtg ctcgccgagg cggcataaat cgccgtgacg atcagcggtc cagtgatcga agttaggctg gtaagagccg cgagcgatcc ttgaagctgt ccctgatggt cgtcatctac ctgcctggac agcatggcct gcaacgcggg catcccgatg ccgccggaag cgagaagaat cataatgggg aaggccatcc agcctcgcgt cgcgaacgcc agcaagacgt agcccagcgc gtcggccgcc atgccggcga taatggcctg cttctcgccg aaacgtttgg tggcgggacc agtgacgaag gcttgagcga gggcgtgcaa gattccgaat accgcaagcg acaggccgat catcgtcgcg ctccagcgaa agcggtcctc gccgaaaatg acccagagcg ctgccggcac ctgtcctacg agttgcatga taaagaagac agtcataagt gcggcgacga tagtcatgcc ccgcgcccac cggaaggagc tgactgggtt gaaggctctc aagggcatcg gtcgacgctc tcccttatgc gactcctgca ttaggaagca gcccagtagt aggttgaggc cgttgagcac cgccgccgca aggaatggtg catgcaagga gatggcgccc aacagtcccc cggccacggg gcctgccacc atacccacgc cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg cgccggtgat gccggccacg atgcgtccgg cgtagaggat cgagatctcg atcccgcgaa at