MyleA.19276.a

Putative cytochrome C reductase cytochrome C component

CENTER ID: MyleA.19276.a
ORGANISM: Mycobacterium leprae Br4923
ASSOCIATED DISEASE: Hansen's disease, leprosy
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

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Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MyleA.19276.a.B2.GE40595 cytochrome c-like domain 64 254
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
UniProt: A0A0H3MQ23

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MKKLGFTRSS RRCSQPQERE QESERSRRRL RRRLSEGLLL LVALTVSGGL AAVLTPTPQV AVADEDSSAL LRTGKQLFDT SCVSCHGANL QGVPDHGPSL IGVGEAAVYF QVSTGRMPAM RGEAQVARKD PIFNESQIDA IGAYIQANGG GPTVARNPDG SVAMQSLRGT DLGRGGDLFR LNCASCHNFT GKGGALSSGK YAPDLGPANE QQILTAMLTG PQNMPKFADR QLSFEAKKDI IGYVRTVIEE RQPGGYSLGG FGPAPEGMAI WIIGMVTAIG LALWIGARA
NT Sequence
ttgaagaaac tgggtttcac tcgatccagt aggcggtgca gtcagccaca agaacgagaa caagagagtg agcgttcgcg gcggcgttta cgccgccgct tgtcggaggg ccttttgcta ctggtagcgc tgaccgtttc tgggggacta gcggcggtcc tgactccgac cccgcaagtg gccgtcgccg atgaggactc atcagctctg ctgcgtaccg gcaagcagct gttcgacacc tcctgcgtgt cgtgccatgg cgccaacctt caaggagttc ccgaccacgg gccgagcctg attggcgtcg gcgaagctgc cgtctatttc caggtatcga ctggtcggat gcctgctatg cgcggtgagg cccaggtggc gcgtaaagac cccattttca acgagtcaca aatcgacgcg atcggtgcct acatccaggc caacggcggt gggccgaccg tagcgcgtaa ccccgacggt agcgttgcga tgcaatcact gcgcggtacc gacctgggtc gcggcggcga cttgttccga ctaaactgcg catcgtgcca caatttcacc ggaaaaggtg gtgcgctgtc gtccggtaag tacgcccccg acctcgggcc cgccaacgaa cagcaaatcc tcaccgcgat gctgaccggc ccgcagaaca tgcccaagtt cgccgaccgc caactttcct tcgaagcgaa gaaggacatc atcggctacg tgcgaaccgt catcgaagaa cgccagccag gtggctacag tctcggtggc ttcggccctg caccggaggg gatggcgatc tggatcatcg ggatggtcac cgccatcggg ctggcactgt ggattggggc acgagcatga
Details for MyleA.19276.a.B2.GE40595
HARVESTED ON: 10/18/2016
SEQUENCED ON: 10/19/2016
EXPECTED MW: 21kDa
OBSERVED MW: 21kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMD EDSSALLRTG KQLFDTSCVS CHGANLQGVP DHGPSLIGVG EAAVYFQVST GRMPAMRGEA QVARKDPIFN ESQIDAIGAY IQANGGGPTV ARNPDGSVAM QSLRGTDLGR GGDLFRLNCA SCHNFTGKGG ALSSGKYAPD LGPANEQQIL TAMLTGPQNM PKFADRQLSF EAKKDIIGYV RTVIEERQPG
Validated NT Sequence
atcttactca cttaacctgg ctggcgttct tcgatgacgg ttcgcacgta gccgatgatg tccttcttcg cttcgaagga aagttggcgg tcggcgaact tgggcatgtt ctgcgggccg gtcagcatcg cggtgaggat ttgctgttcg ttggcgggcc cgaggtcggg ggcgtactta ccggacgaca gcgcaccacc ttttccggtg aaattgtggc acgatgcgca gtttagtcgg aacaagtcgc cgccgcgacc caggtcggta ccgcgcagtg attgcatcgc aacgctaccg tcggggttac gcgctacggt cggcccaccg ccgttggcct ggatgtaggc accgatcgcg tcgatttgtg actcgttgaa aatggggtct ttacgcgcca cctgggcctc accgcgcata gcaggcatcc gaccagtcga tacctggaaa tagacggcag cttcgccgac gccaatcagg ctcggcccgt ggtcgggaac tccttgaagg ttggcgccat ggcacgacac gcaggaggtg tcgaacagct gcttgccggt acgcagcaga gctgatgagt cctcatccat atggtggtgg tggtggtgag ccat
Expected Protein Sequence
MAHHHHHHDE DSSALLRTGK QLFDTSCVSC HGANLQGVPD HGPSLIGVGE AAVYFQVSTG RMPAMRGEAQ VARKDPIFNE SQIDAIGAYI QANGGGPTVA RNPDGSVAMQ SLRGTDLGRG GDLFRLNCAS CHNFTGKGGA LSSGKYAPDL GPANEQQILT AMLTGPQNMP KFADRQLSFE AKKDIIGYVR TVIEERQPG
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgatgagg actcatcagc tctgctgcgt accggcaagc agctgttcga cacctcctgc gtgtcgtgcc atggcgccaa ccttcaagga gttcccgacc acgggccgag cctgattggc gtcggcgaag ctgccgtcta tttccaggta tcgactggtc ggatgcctgc tatgcgcggt gaggcccagg tggcgcgtaa agaccccatt ttcaacgagt cacaaatcga cgcgatcggt gcctacatcc aggccaacgg cggtgggccg accgtagcgc gtaaccccga cggtagcgtt gcgatgcaat cactgcgcgg taccgacctg ggtcgcggcg gcgacttgtt ccgactaaac tgcgcatcgt gccacaattt caccggaaaa ggtggtgcgc tgtcgtccgg taagtacgcc cccgacctcg ggcccgccaa cgaacagcaa atcctcaccg cgatgctgac cggcccgcag aacatgccca agttcgccga ccgccaactt tccttcgaag cgaagaagga catcatcggc tacgtgcgaa ccgtcatcga agaacgccag ccaggttgag taagatagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aattcttgaa gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat acactccgct atcgctacgt gactgggtca tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgaggcagc tgcggtaaag ctcatcagcg tggtcgtgaa gcgattcaca gatgtctgcc tgttcatccg cgtccagctc gttgagtttc tccagaagcg ttaatgtctg gcttctgata aagcgggcca tgttaagggc ggttttttcc tgtttggtca ctgatgcctc cgtgtaaggg ggatttctgt tcatgggggt aatgataccg atgaaacgag agaggatgct cacgatacgg gttactgatg atgaacatgc ccggttactg gaacgttgtg agggtaaaca actggcggta tggatgcggc gggaccagag aaaaatcact cagggtcaat gccagcgctt cgttaataca gatgtaggtg ttccacaggg tagccagcag catcctgcga tgcagatccg gaacataatg gtgcagggcg ctgacttccg cgtttccaga ctttacgaaa cacggaaacc gaagaccatt catgttgttg ctcaggtcgc agacgttttg cagcagcagt cgcttcacgt tcgctcgcgt atcggtgatt cattctgcta accagtaagg caaccccgcc agcctagccg ggtcctcaac gacaggagca cgatcatgcg cacccgtggc caggacccaa cgctgcccga gatgcgccgc gtgcggctgc tggagatggc ggacgcgatg gatatgttct gccaagggtt ggtttgcgca ttcacagttc tccgcaagaa ttgattggct ccaattcttg gagtggtgaa tccgttagcg aggtgccgcc ggcttccatt caggtcgagg tggcccggct ccatgcaccg cgacgcaacg cggggaggca gacaaggtat agggcggcgc ctacaatcca tgccaacccg ttccatgtgc tcgccgaggc ggcataaatc gccgtgacga tcagcggtcc agtgatcgaa gttaggctgg taagagccgc gagcgatcct tgaagctgtc cctgatggtc gtcatctacc tgcctggaca gcatggcctg caacgcgggc atcccgatgc cgccggaagc gagaagaatc ataatgggga aggccatcca gcctcgcgtc gcgaacgcca gcaagacgta gcccagcgcg tcggccgcca tgccggcgat aatggcctgc ttctcgccga aacgtttggt ggcgggacca gtgacgaagg cttgagcgag ggcgtgcaag attccgaata ccgcaagcga caggccgatc atcgtcgcgc tccagcgaaa gcggtcctcg ccgaaaatga cccagagcgc tgccggcacc tgtcctacga gttgcatgat aaagaagaca gtcataagtg cggcgacgat agtcatgccc cgcgcccacc ggaaggagct gactgggttg aaggctctca agggcatcgg tcgacgctct cccttatgcg actcctgcat taggaagcag cccagtagta ggttgaggcc gttgagcacc gccgccgcaa ggaatggtgc atgcaaggag atggcgccca acagtccccc ggccacgggg cctgccacca tacccacgcc gaaacaagcg ctcatgagcc cgaagtggcg agcccgatct tccccatcgg tgatgtcggc gatataggcg ccagcaaccg cacctgtggc gccggtgatg ccggccacga tgcgtccggc gtagaggatc gagatctcga tcccgcgaaa t