MyleA.18665.a

Uncharacterized protein

CENTER ID: MyleA.18665.a
ORGANISM: Mycobacterium leprae Br4923
ASSOCIATED DISEASE: Hansen's disease, leprosy
CURRENT STATUS: soluble
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MyleA.18665.a.B2.GE40128 MythA crystallizable fragment 50 420
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|561304.4.peg.3617
RefSeq: YP_002503907.1
UniProt: A0A0H3MVT2

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSSTNNHQVV KLDRVPLPVE AARIAATYWQ ITRAAARVAT KLAGKGPWQQ KVIREIPQTF ANLGPTYVKF GQIIASSPSA FGESLAREFR GLLDQVPPAD TNEVHKLFVE ELGDAPSKLF ASFSEEPFAS ASIAQVHSAI LHSGEHVVVK IQRPGIRRRV AADLQILKRF AQAVELAKLG RRLSAQDVVA DFSDNLAEEL NFRLEAQSME AWGSYLHTSP LGKNIRVPKV HWEFTGERVL TMEQVHGIRI DNADAIRKSG FDGVELVKAL LFSVFEGGLR HGLFHGDLHA GNLHVDDQGR IVFFDFGIMG RIDPRTRWLL RELVFALLVK KDHAAAGKIV VLMGAVGTVK PEARAAKDLE NFTTPLAMKT LGDMSYTDIG RQLSALSDAY NVKLPRELVL IGKQFLYVER YMKLLAPEWQ IMLDPELTGY FANFMVEVSR EHQSDVEI
NT Sequence
atgagttcca ccaataacca ccaggtggtc aagctagacc gagtgccctt acctgtcgaa gcggcccgga tagcagccac atattggcaa atcacccgcg ctgccgctcg cgtcgcaacc aagctggcgg gcaagggtcc gtggcagcaa aaggtgatca gggaaatccc gcagaccttt gctaacctag ggccgacgta cgttaagttc ggacagatca tagcgtccag ccccagcgca tttggtgagt cgctggcgcg cgaattccgt ggcctgctcg accaagtgcc accagccgac acgaacgagg tgcacaagct gttcgtcgag gagcttggcg acgcgccttc caaactgttc gccagcttct ccgaagagcc gttcgcgtcg gcgtccatcg ctcaggtaca ttccgcgatc ctgcacagcg gcgagcacgt cgtcgtaaag atccagcgac ccggcatccg acgccgagtc gccgccgacc tgcaaatcct gaagcgcttc gcgcaagcag tcgaactagc caagctgggc cgtcggctct cggcgcaaga cgtcgtagcg gatttttccg acaaccttgc cgaagagctg aatttccgtc tcgaggcaca gtcgatggaa gcgtggggct catacctgca cacctcgccg ctaggcaaga acatccgcgt accgaaggtg cactgggaat tcacgggcga acgggtgttg acgatggagc aggtacatgg catccgcata gacaatgcgg atgccatccg caagtctggc ttcgacggcg tcgagctggt caaggcgcta ttgttctcgg tgtttgaggg cggtctgcga cacggcctgt tccatggcga cctgcacgcg ggcaacctgc atgttgacga ccaaggtcgc atcgtgttct tcgacttcgg gatcatgggc cgcatcgatc cgcgcacccg gtggttacta cgcgaactgg tatttgccct gctggtgaaa aaggaccacg ctgctgctgg caaaatcgtc gtgttgatgg gcgccgtggg caccgtgaaa cccgaggctc gggctgccaa agacctggaa aacttcacca cgcctctggc catgaaaacc ctcggcgaca tgtcctacac cgatatcggc cggcagctgt cggcgctgtc cgacgcctac aacgttaagt tgccccgcga actggtgctg atcggcaagc aattcctcta cgtcgagcgg tacatgaagc tgctggcgcc ggaatggcag ataatgttgg atccggagct gacagggtac ttcgcaaact ttatggtcga agtcagccgt gagcatcaat ccgatgtgga gatctag
Details for MyleA.18665.a.B2.GE40128
HARVESTED ON: 4/6/2016
SEQUENCED ON: 4/13/2016
EXPECTED MW: 42kDa
OBSERVED MW: 45kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY: 100
PERCENT COVERAGE: 98
Validated AA Sequence
MAHHHHHHMQ KVIREIPQTF ANLGPTYVKF GQIIASSPSA FGESLAREFR GLLDQVPPAD TNEVHKLFVE ELGDAPSKLF ASFSEEPFAS ASIAQVHSAI LHSGEHVVVK IQRPGIRRRV AADLQILKRF AQAVELAKLG RRLSAQDVVA DFSDNLAEEL NFRLEAQSME AWGSYLHTSP LGKNIRVPKV HWEFTGERVL TMEQVHGIRI DNADAIRKSG FDGVELVKAL LFSVFEGGLR HGLFHGDLHA GNLHVDDQGR IVFFDFGIMG RIDPRTRWLL RELVFALLVK KDHAAAGKIV VLMGAVGTVK PEARAAKDLE NFTTPLAMKT LGDMSYTDIG RQLSALSDAY NVKLPRELVL IGKQFLYVER YM
Validated NT Sequence
tcatgtaccg ctcgacgtag aggaattgct tgccgatcag caccagttcg cggggcaact taacgttgta ggcgtcggac agcgccgaca gctgccggcc gatatcggtg taggacatgt cgccgagggt tttcatggcc agaggcgtgg tgaagttttc caggtctttg gcagcccgag cctcgggttt cacggtgccc acggcgccca tcaacacgac gattttgcca gcagcagcgt ggtccttttt caccagcagg gcaaatacca gttcgcgtag taaccaccgg gtgcgcggat cgatgcggcc catgatcccg aagtcgaaga acacgatgcg accttggtcg tcaacatgca ggttgcccgc gtgcaggtcg ccatggaaca ggccgtgtcg cagaccgccc tcaaacaccg agaacaatag cgccttgacc agctcgacgc cgtcgaagcc agacttgcgg atggcatccg cattgtctat gcggatgcca tgtacctgct ccatcgtcaa cacccgttcg cccgtgaatt cccagtgcac cttcggtacg cggatgttct tgcctagcgg cgaggtgtgc aggtatgagc cccacgcttc catcgactgt gcctcgagac ggaaattcag ctcttcggca aggttgtcgg aaaaatccgc tacgacgtct tgcgccgaga gccgacggcc cagcttggct agttcgactg cttgcgcgaa gcgcttcagg atttgcaggt cggcggcgac tcggcgtcgg atgccgggtc gctggatctt tacgacgacg tgctcgccgc tgtgcaggat cgcggaatgt acctgagcga tggacgccga cgcgaacggc tcttcggaga agctggcgaa cagtttggaa ggcgcgtcgc caagctcctc gacgaacagc ttgtgcacct cgttcgtgtc ggctggtggc acttggtcga gcaggccacg gaattcgcgc gccagcgact caccaaatgc gctggggctg gacgctatga tctgtccgaa cttaacgtac gtcggcccta ggttagcaaa ggtctgcggg atttccctga tcaccttttg catatggtgg tggtggtggt gagccat
Expected Protein Sequence
MAHHHHHHQK VIREIPQTFA NLGPTYVKFG QIIASSPSAF GESLAREFRG LLDQVPPADT NEVHKLFVEE LGDAPSKLFA SFSEEPFASA SIAQVHSAIL HSGEHVVVKI QRPGIRRRVA ADLQILKRFA QAVELAKLGR RLSAQDVVAD FSDNLAEELN FRLEAQSMEA WGSYLHTSPL GKNIRVPKVH WEFTGERVLT MEQVHGIRID NADAIRKSGF DGVELVKALL FSVFEGGLRH GLFHGDLHAG NLHVDDQGRI VFFDFGIMGR IDPRTRWLLR ELVFALLVKK DHAAAGKIVV LMGAVGTVKP EARAAKDLEN FTTPLAMKTL GDMSYTDIGR QLSALSDAYN VKLPRELVLI GKQFLYVERY MKLLAPEWQ
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catcaaaagg tgatcaggga aatcccgcag acctttgcta acctagggcc gacgtacgtt aagttcggac agatcatagc gtccagcccc agcgcatttg gtgagtcgct ggcgcgcgaa ttccgtggcc tgctcgacca agtgccacca gccgacacga acgaggtgca caagctgttc gtcgaggagc ttggcgacgc gccttccaaa ctgttcgcca gcttctccga agagccgttc gcgtcggcgt ccatcgctca ggtacattcc gcgatcctgc acagcggcga gcacgtcgtc gtaaagatcc agcgacccgg catccgacgc cgagtcgccg ccgacctgca aatcctgaag cgcttcgcgc aagcagtcga actagccaag ctgggccgtc ggctctcggc gcaagacgtc gtagcggatt tttccgacaa ccttgccgaa gagctgaatt tccgtctcga ggcacagtcg atggaagcgt ggggctcata cctgcacacc tcgccgctag gcaagaacat ccgcgtaccg aaggtgcact gggaattcac gggcgaacgg gtgttgacga tggagcaggt acatggcatc cgcatagaca atgcggatgc catccgcaag tctggcttcg acggcgtcga gctggtcaag gcgctattgt tctcggtgtt tgagggcggt ctgcgacacg gcctgttcca tggcgacctg cacgcgggca acctgcatgt tgacgaccaa ggtcgcatcg tgttcttcga cttcgggatc atgggccgca tcgatccgcg cacccggtgg ttactacgcg aactggtatt tgccctgctg gtgaaaaagg accacgctgc tgctggcaaa atcgtcgtgt tgatgggcgc cgtgggcacc gtgaaacccg aggctcgggc tgccaaagac ctggaaaact tcaccacgcc tctggccatg aaaaccctcg gcgacatgtc ctacaccgat atcggccggc agctgtcggc gctgtccgac gcctacaacg ttaagttgcc ccgcgaactg gtgctgatcg gcaagcaatt cctctacgtc gagcggtaca tgaagctgct ggcgccggaa tggcagtgag taagatagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aattcttgaa gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat acactccgct atcgctacgt gactgggtca tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgaggcagc tgcggtaaag ctcatcagcg tggtcgtgaa gcgattcaca gatgtctgcc tgttcatccg cgtccagctc gttgagtttc tccagaagcg ttaatgtctg gcttctgata aagcgggcca tgttaagggc ggttttttcc tgtttggtca ctgatgcctc cgtgtaaggg ggatttctgt tcatgggggt aatgataccg atgaaacgag agaggatgct cacgatacgg gttactgatg atgaacatgc ccggttactg gaacgttgtg agggtaaaca actggcggta tggatgcggc gggaccagag aaaaatcact cagggtcaat gccagcgctt cgttaataca gatgtaggtg ttccacaggg tagccagcag catcctgcga tgcagatccg gaacataatg gtgcagggcg ctgacttccg cgtttccaga ctttacgaaa cacggaaacc gaagaccatt catgttgttg ctcaggtcgc agacgttttg cagcagcagt cgcttcacgt tcgctcgcgt atcggtgatt cattctgcta accagtaagg caaccccgcc agcctagccg ggtcctcaac gacaggagca cgatcatgcg cacccgtggc caggacccaa cgctgcccga gatgcgccgc gtgcggctgc tggagatggc ggacgcgatg gatatgttct gccaagggtt ggtttgcgca ttcacagttc tccgcaagaa ttgattggct ccaattcttg gagtggtgaa tccgttagcg aggtgccgcc ggcttccatt caggtcgagg tggcccggct ccatgcaccg cgacgcaacg cggggaggca gacaaggtat agggcggcgc ctacaatcca tgccaacccg ttccatgtgc tcgccgaggc ggcataaatc gccgtgacga tcagcggtcc agtgatcgaa gttaggctgg taagagccgc gagcgatcct tgaagctgtc cctgatggtc gtcatctacc tgcctggaca gcatggcctg caacgcgggc atcccgatgc cgccggaagc gagaagaatc ataatgggga aggccatcca gcctcgcgtc gcgaacgcca gcaagacgta gcccagcgcg tcggccgcca tgccggcgat aatggcctgc ttctcgccga aacgtttggt ggcgggacca gtgacgaagg cttgagcgag ggcgtgcaag attccgaata ccgcaagcga caggccgatc atcgtcgcgc tccagcgaaa gcggtcctcg ccgaaaatga cccagagcgc tgccggcacc tgtcctacga gttgcatgat aaagaagaca gtcataagtg cggcgacgat agtcatgccc cgcgcccacc ggaaggagct gactgggttg aaggctctca agggcatcgg tcgacgctct cccttatgcg actcctgcat taggaagcag cccagtagta ggttgaggcc gttgagcacc gccgccgcaa ggaatggtgc atgcaaggag atggcgccca acagtccccc ggccacgggg cctgccacca tacccacgcc gaaacaagcg ctcatgagcc cgaagtggcg agcccgatct tccccatcgg tgatgtcggc gatataggcg ccagcaaccg cacctgtggc gccggtgatg ccggccacga tgcgtccggc gtagaggatc gagatctcga tcccgcgaaa t