SSGCID: Target Page

MyleA.18381.a

CONSERVED HYPOTHETICAL PROTEIN

CENTER ID:	MyleA.18381.a
ORGANISM:	Mycobacterium leprae Br4923
ASSOCIATED DISEASE:	Hansen's disease, leprosy
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MyleA.18381.a.A1.GE34197	full length		1	259

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|561304.4.peg.5230
RefSeq:	YP_002504356.1
UniProt:	A0A0H3N199

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MSAQITPLRL EAFEQLPKHA RRCVFWEVDP ATLGNQDHLV DLEFEKEAWL SMVMLEWGSC GQVATAIMDE CRQSDAFKHL EPPCLGYMLY APPRVVPRAY RFPTAPVSAD AVLLTSMGVE PGQVAAGLPQ SLISQVIDEL VRRGVRALEA FGRTEVATEL QDPRTVAPDV RPVLEALGDC SVDHCIIAAD FLKAVGFVVV APHQYFPRLR LELDKGFGWK AEVEAALERL LADAQLQQPV GAGAVVKQHS ESRNIWLIR

NT Sequence

gtgtctgctc aaatcacacc gctacgtctc gaagctttcg agcagctccc taagcatgca cgccggtgcg tcttttggga ggtcgacccg gcgacccttg gcaaccagga ccacctcgtt gatcttgaat tcgaaaagga agcttggttg tcgatggtaa tgttggaatg gggttcgtgt ggtcaggtcg caacggcgat tatggacgag tgccgccaat ctgatgcatt caaacactta gagccgccgt gcttggggta tatgctttat gccccaccac gggtagtgcc ccgagcatat cggtttccca ctgcgccagt gtccgctgat gccgtcctac tgacttcgat gggcgtcgag ccagggcaag tcgcggctgg tcttccacag agtctcatca gccaggtaat tgacgaactg gttcggcgtg gagtccgggc gttagaggct tttggccgca ccgaagtggc caccgagttg caggatcccc ggacagtggc ccccgatgtt cggccggtat tggaggctct cggtgactgt tcggtcgacc actgcatcat tgccgcggac ttcttgaagg ccgtcggttt cgtcgtcgtt gcaccgcacc aatatttccc tcggctacgc cttgagttgg acaagggctt tggctggaaa gctgaagtcg aggccgcctt agagcgtctg ctggcggacg ctcagttaca acagccagtc ggggccggag ctgtagttaa gcaacattct gaaagccgca atatatggct tatacgttaa

Details for MyleA.18381.a.A1.GE34197

HARVESTED ON:	12/6/2011
SEQUENCED ON:	12/8/2011
EXPECTED MW:	31kDa
OBSERVED MW:	31kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Many (50-100)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	99
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SMVSAQITPL RLEAFEQLPK HARRCVFWEV DPATLGNQDH LVDLEFEKEA WLSMVMLEWG SCGQVATAIM DECRQSDAFK HLEPPCLGYM LYAPPRVVPR AYRFPTAPVS ADAVLLTSMG VEPGQVAAGL PQSLISQVID ELVRRGVRAL EAFGRTEVAT ELQDPRTVAP DVRPVLEALG DCSVDHCIIA ADFLKAVGFV VVAPHQYFPR LRLELDKGFG WKAEVEAALE RLLADAQLQQ PVGAGAVVKQ HSESRNIWXI R

Validated NT Sequence

attttgttta ctttaagang gagatatacc atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatggtgt ctgctcaaat cacaccgcta cgtctcgaag ctttcgagca gctccctaag catgcacgcc ggtgcgtctt ttgggaggtc gacccggcga cccttggcaa ccaggaccac ctcgttgatc ttgaattcga aaaggaagct tggttgtcga tggtaatgtt ggaatggggt tcgtgtggtc aggtcgcaac ggcgattatg gacgagtgcc gccaatctga tgcattcaaa cacttagagc cgccgtgctt ggggtatatg ctttatgccc caccacgggt agtgccccga gcatatcggt ttcccactgc gccagtgtcc gctgatgccg tcctactgac ttcgatgggc gtcgagccag ggcaagtcgc ggctggtctt ccacagagtc tcatcagcca ggtaattgac gaactggttc ggcgtggagt ccgggcgtta gaggcttttg gccgcaccga agtggccacc gagttgcagg atccccggac agtggccccc gatgttcggc cggtattgga ggctctcggt gactgttcgg tcgaccactg catcattgcc gcggacttct tgaaggccgt cggtttcgtc gtcgttgcac cgcaccaata tttccctcgg ctacgccttg agttggacaa gggctttggc tggaaagctg aagtcgaggc cgccttagag cgtctgctgg cggacgctca gttacaacag ccagtcgggg ccggagctgt agttaagcaa cattctgaaa gccgcaatat atggnttata cgttaataaa cagcacgaac aagttctgca gccaagcttc tcgaggancc ngctgctaac aaagcccgaa aggaanctga nttngctncn gnnnnnnnnn nnnnnnnnaa aaaaaa

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtgtctgct caaatcacac cgctacgtct cgaagctttc gagcagctcc ctaagcatgc acgccggtgc gtcttttggg aggtcgaccc ggcgaccctt ggcaaccagg accacctcgt tgatcttgaa ttcgaaaagg aagcttggtt gtcgatggta atgttggaat ggggttcgtg tggtcaggtc gcaacggcga ttatggacga gtgccgccaa tctgatgcat tcaaacactt agagccgccg tgcttggggt atatgcttta tgccccacca cgggtagtgc cccgagcata tcggtttccc actgcgccag tgtccgctga tgccgtccta ctgacttcga tgggcgtcga gccagggcaa gtcgcggctg gtcttccaca gagtctcatc agccaggtaa ttgacgaact ggttcggcgt ggagtccggg cgttagaggc ttttggccgc accgaagtgg ccaccgagtt gcaggatccc cggacagtgg cccccgatgt tcggccggta ttggaggctc tcggtgactg ttcggtcgac cactgcatca ttgccgcgga cttcttgaag gccgtcggtt tcgtcgtcgt tgcaccgcac caatatttcc ctcggctacg ccttgagttg gacaagggct ttggctggaa agctgaagtc gaggccgcct tagagcgtct gctggcggac gctcagttac aacagccagt cggggccgga gctgtagtta agcaacattc tgaaagccgc aatatatggc ttatacgtta aacagcacga acaagttctg cagccaagct tctcgaggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga a