MyleA.18379.a

PPE FAMILY PROTEIN

CENTER ID: MyleA.18379.a
ORGANISM: Mycobacterium leprae Br4923
ASSOCIATED DISEASE: Hansen's disease, leprosy
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MyleA.18379.a.A1.GE34196 full length 1 302
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|561304.4.peg.76
RefSeq: YP_002502795.1
UniProt: A0A0H3MXS4

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTWPMLWPAS VPSECPPNYW HTPAPSAKCE PEQAAVAPIA AAKPMITWLQ SAAEQTTTQA EAHRQAMAST PGMAVITENH ITQAILATIN FFGINMAPIA FTEAGDFICM RTQTALAMNS YQAETLLNTA FQKLEPMAAI LNPSSYSPPS ALTSQVNQFT QMISGFSAAL PSTQVLQQTV GQVAELARPM QQVKSLFTSI DSTGVYTSAQ RGDTESAHRI GLFGASTLSS HPLVGITGTT TDTRLLCAES LPSASGSLAW TPLMTQFQLI DKSIAPEPRQ RVMLPPWAAG SPGHNAQDGG TT
NT Sequence
atgacttggc cgatgttgtg gcccgcatca gtcccgagcg agtgtccgcc gaattattgg cacaccccag ctccctcagc gaagtgtgaa ccagagcaag cagcggtggc gccgattgcg gctgccaagc ccatgattac ctggctgcaa tccgctgcag agcagacaac cacgcaggcc gaagcgcaca ggcaagcaat ggcaagcacg ccgggaatgg ccgtgattac cgaaaaccac atcacccagg caatccttgc caccatcaac ttctttggca tcaatatggc gccaatcgcc tttacggaag cgggcgattt catttgcatg cgaacccaga ccgccttagc aatgaactcc taccaggcgg aaaccctgct gaacacggct ttccaaaagc ttgagccaat ggccgcgatc cttaatccgt cgagctactc cccgccatca gccctaacgt cccaggtgaa ccaattcacg cagatgatat ccggattttc cgctgccctg ccttcaaccc aggtgctgca gcaaacggtt gggcaggtcg cggagcttgc tcgcccgatg cagcaggtga agtcactctt cacctctata gatagcactg gcgtttacac gagtgcccaa cgcggcgaca cggaaagcgc ccatcgcatt ggtctttttg gtgccagtac actatccagc catcctttgg tcggtataac gggcacaacc accgacacaa gactgctgtg cgcggagtcg ctgcccagtg cgagcgggtc gttggcgtgg acaccgctga tgacccagtt ccagttgatc gataagtcga ttgcgccgga gcctcggcaa cgggtgatgc tgcccccgtg ggcggcaggc tctccggggc acaacgccca agacggtgga acgacctga
Details for MyleA.18379.a.A1.GE34196
HARVESTED ON: 12/6/2011
SEQUENCED ON: 12/13/2011
EXPECTED MW: 34kDa
OBSERVED MW: 34kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 98
PERCENT COVERAGE: 89
Validated AA Sequence
MGTLEAQTQG PGSMTWPMLW PASVPSECPP NYWHTPAPSA KCEPEQAAVA PIAAAKPMIT WLQSAAEQTT TQAEAHRQAM ASTPGMAVIT ENHITQAILA TINFFGINMA PIAFTEAGDF ICMRTQTALA MNSYQAETLL NTAFQKLEPM AAILNPSSYS PPSALTSQVN QFTQMISGFS AALPSTQVLQ QTVGQVAELA RPMQQVKSLF TSIDSTGVYT SAQRGDTESA HRIGLFGAST LSSHPLVGIT GTTTDTRLLC AXSLPSASGS LAWXPLXTQF QLIDKSIXP
Validated NT Sequence
tggctcatca ccatcaccat catatgggta ccctggaagc tcagacccag ggtcctggtt cgatgacttg gccgatgttg tggcccgcat cagtcccgag cgagtgtccg ccgaattatt ggcacacccc agctccctca gcgaagtgtg aaccagagca agcagcggtg gcgccgattg cggctgccaa gcccatgatt acctggctgc aatccgctgc agagcagaca accacgcagg ccgaagcgca caggcaagca atggcaagca cgccgggaat ggccgtgatt accgaaaacc acatcaccca ggcaatcctt gccaccatca acttctttgg catcaatatg gcgccaatcg cctttacgga agcgggcgat ttcatttgca tgcgaaccca gaccgcctta gcaatgaact cctaccaggc ggaaaccctg ctgaacacgg ctttccaaaa gcttgagcca atggccgcga tccttaatcc gtcgagctac tccccgccat cagccctaac gtcccaggtg aaccaattca cgcagatgat atccggattt tccgctgccc tgccttcaac ccaggtgctg cagcaaacgg ttgggcaggt cgcggagctt gctcgcccga tgcagcaggt gaagtcactc ttcacctcta tagatagcac tggcgtttac acgagtgccc aacgcggcga cacggaaagc gcccatcgca ttggtctttt tggtgccagt acactatcca gccatccttt ggtcggtata acgggcacaa ccaccgacac aagactgctg tgcgcggant cgctgcccag tgcgagcggg tcgttggcgt ggnnaccgct gangacccag ttccagttga tcgataagtc gattgngccg ga
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMTWPMLWPA SVPSECPPNY WHTPAPSAKC EPEQAAVAPI AAAKPMITWL QSAAEQTTTQ AEAHRQAMAS TPGMAVITEN HITQAILATI NFFGINMAPI AFTEAGDFIC MRTQTALAMN SYQAETLLNT AFQKLEPMAA ILNPSSYSPP SALTSQVNQF TQMISGFSAA LPSTQVLQQT VGQVAELARP MQQVKSLFTS IDSTGVYTSA QRGDTESAHR IGLFGASTLS SHPLVGITGT TTDTRLLCAE SLPSASGSLA WTPLMTQFQL IDKSIAPEPR QRVMLPPWAA GSPGHNAQDG GTT
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gacttggccg atgttgtggc ccgcatcagt cccgagcgag tgtccgccga attattggca caccccagct ccctcagcga agtgtgaacc agagcaagca gcggtggcgc cgattgcggc tgccaagccc atgattacct ggctgcaatc cgctgcagag cagacaacca cgcaggccga agcgcacagg caagcaatgg caagcacgcc gggaatggcc gtgattaccg aaaaccacat cacccaggca atccttgcca ccatcaactt ctttggcatc aatatggcgc caatcgcctt tacggaagcg ggcgatttca tttgcatgcg aacccagacc gccttagcaa tgaactccta ccaggcggaa accctgctga acacggcttt ccaaaagctt gagccaatgg ccgcgatcct taatccgtcg agctactccc cgccatcagc cctaacgtcc caggtgaacc aattcacgca gatgatatcc ggattttccg ctgccctgcc ttcaacccag gtgctgcagc aaacggttgg gcaggtcgcg gagcttgctc gcccgatgca gcaggtgaag tcactcttca cctctataga tagcactggc gtttacacga gtgcccaacg cggcgacacg gaaagcgccc atcgcattgg tctttttggt gccagtacac tatccagcca tcctttggtc ggtataacgg gcacaaccac cgacacaaga ctgctgtgcg cggagtcgct gcccagtgcg agcgggtcgt tggcgtggac accgctgatg acccagttcc agttgatcga taagtcgatt gcgccggagc ctcggcaacg ggtgatgctg cccccgtggg cggcaggctc tccggggcac aacgcccaag acggtggaac gacctaaaca gcacgaacaa gttctgcagc caagcttctc gaggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaa