SSGCID: Target Page

MyleA.18373.a

POSSIBLE RNA METHYLTRANSFERASE

CENTER ID:	MyleA.18373.a
ORGANISM:	Mycobacterium leprae Br4923
ASSOCIATED DISEASE:	Hansen's disease, leprosy
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MyleA.18373.a.A1.GE34194	full length		1	281

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|561304.4.peg.2432
RefSeq:	YP_002503574.1
UniProt:	A0A0H3MV80

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MSATGPFTVG ERVQLTDTKG RHYTMSLTPG GEFHTHRGSI AHDGIIGLEQ GSVVKSSTGA LFLVLRPLLI DYVMSMPRGP QVIYPKDAAQ IVHEGDIFPG ARVLEAGAGS GALTCSLLRA VGPQGQVISY ELRTDHAEHA RRNVSNFFCV SGAEGRLSDN WQLIVSDVAN SELPDGSVDR VVLDMLAPWE VLDTVSRLVI AGGVLVIYVA TVTQLSRAVE ALRVQQCWVE PRAWETLQRG WNVVGLAVRP EHLMRGHTAF LILARRLAPG AVAPAPWGRK R

NT Sequence

gtgtccgcaa ctggcccgtt caccgttggc gagcgcgtcc agctcaccga cactaaaggc cgccactaca cgatgtcgct cacccctggt ggagaattcc acactcatcg cggctcgatt gcccacgatg ggatcatcgg cttggaacag ggcagcgtgg tgaaatccag caccggtgcc ctgttcttgg tcttgcgccc gttgctgatc gattacgtga tgtcgatgcc gcgcgggcca caggtgatct accccaagga tgccgctcag atcgtgcacg aaggtgatat ctttcccggt gcccgggtac tggaggccgg ggccggatcc ggtgcgctga cgtgctcgct gctccgagcg gtcgggccgc aggggcaggt gatttcctac gaactgcgca ccgatcacgc tgaacacgct cgtcgcaatg tgtctaactt cttttgtgtc tccggcgccg aaggccgact atcggacaat tggcagctca tcgttagtga tgttgcgaat tcagagttgc ccgacggttc cgtcgaccgg gtggtgttag acatgctggc gccgtgggag gtgctggaca cagtctcgcg gctggtgatc gccggcggcg tattggtgat ctacgtggcc accgtcaccc agctgtcgcg ggctgtggag gctctgcggg ttcagcaatg ttgggttgaa ccgcgggcgt gggaaacgct tcagaggggt tggaacgtgg ttggtctggc ggttcggcca gagcatttga tgcgtgggca caccgcgttc ctgattttgg cccgccggct ggcgcccggc gctgtcgcgc cggcgccgtg gggtcgcaaa cggtga

Details for MyleA.18373.a.A1.GE34194

HARVESTED ON:	12/6/2011
SEQUENCED ON:	12/8/2011
EXPECTED MW:	32kDa
OBSERVED MW:	33kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Many (50-100)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	99
PERCENT COVERAGE:	82

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SMVSATGPFT VGERVQLTDT KGRHYTMSLT PGGEFHTHRG SIAHDGIIGL EQGSVVKSST GALFLVLRPL LIDYVMSMPR GPQVIYPKDA AQIVHEGDIF PGARVLEAGA GSGALTCSLL RAVGPQGQVI SYELRTDHAE HARRNVSNFF CVSGAEGRLS DNWQLIVSDV ANSELPDGSV DRVVLDMLAP WEVLDTVSRL VIAGGVLXIY VATVTQLSRA VEALRVQQCW G

Validated NT Sequence

tanttttgtt tactttanga nggagatata ccatggctca tcaccatcac catcatatgg gtaccctgga agctcagacc cagggtcctg gttcgatggt gtccgcaact ggcccgttca ccgttggcga gcgcgtccag ctcaccgaca ctaaaggccg ccactacacg atgtcgctca cccctggtgg agaattccac actcatcgcg gctcgattgc ccacgatggg atcatcggct tggaacaggg cagcgtggtg aaatccagca ccggtgccct gttcttggtc ttgcgcccgt tgctgatcga ttacgtgatg tcgatgccgc gcgggccaca ggtgatctac cccaaggatg ccgctcagat cgtgcacgaa ggtgatatct ttcccggtgc ccgggtactg gaggccgggg ccggatccgg tgcgctgacg tgctcgctgc tccgagcggt cgggccgcag gggcaggtga tttcctacga actgcgcacc gatcacgctg aacacgctcg tcgcaatgtg tctaacttct tttgtgtctc cggcgccgaa ggccgactat cggacaattg gcagctcatc gttagtgatg ttgcgaattc agagttgccc gacggttccg tcgaccgggt ggtgttagac atgctggcgc cgtgggaggt gctggacaca gtctcgcggc tggtgatcgc cggcggcgta ttgntgatct acgtggccac cgtcacccag ctgtcgcggg ctgtggaggc tctgcgggtt cagcaatgtt ggggttgaac cgcgggcgtg ggaaacgctt canaggggtt ggaacgtggt tggtctggcg gttcggccag agcatttgat gcgtgggcac accgcgttcc tgatttggcc cgccggctgg cgcccggngc tgtcgcgccn nnnccgtggg gtcgcaaacg gtannaaaca gcacgannaa gttctgcagc caagcttnnn nagganccgg ctgctaacaa agcccgaann ancnna

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtgtccgca actggcccgt tcaccgttgg cgagcgcgtc cagctcaccg acactaaagg ccgccactac acgatgtcgc tcacccctgg tggagaattc cacactcatc gcggctcgat tgcccacgat gggatcatcg gcttggaaca gggcagcgtg gtgaaatcca gcaccggtgc cctgttcttg gtcttgcgcc cgttgctgat cgattacgtg atgtcgatgc cgcgcgggcc acaggtgatc taccccaagg atgccgctca gatcgtgcac gaaggtgata tctttcccgg tgcccgggta ctggaggccg gggccggatc cggtgcgctg acgtgctcgc tgctccgagc ggtcgggccg caggggcagg tgatttccta cgaactgcgc accgatcacg ctgaacacgc tcgtcgcaat gtgtctaact tcttttgtgt ctccggcgcc gaaggccgac tatcggacaa ttggcagctc atcgttagtg atgttgcgaa ttcagagttg cccgacggtt ccgtcgaccg ggtggtgtta gacatgctgg cgccgtggga ggtgctggac acagtctcgc ggctggtgat cgccggcggc gtattggtga tctacgtggc caccgtcacc cagctgtcgc gggctgtgga ggctctgcgg gttcagcaat gttgggttga accgcgggcg tgggaaacgc ttcagagggg ttggaacgtg gttggtctgg cggttcggcc agagcatttg atgcgtgggc acaccgcgtt cctgattttg gcccgccggc tggcgcccgg cgctgtcgcg ccggcgccgt ggggtcgcaa acggtaaaca gcacgaacaa gttctgcagc caagcttctc gaggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaa