MyleA.18344.a
PROBABLE MALATE SYNTHASE G GLCB
| CENTER ID: | MyleA.18344.a |
| ORGANISM: | Mycobacterium leprae Br4923 |
| ASSOCIATED DISEASE: | Hansen's disease, leprosy |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | True |
| NIH RISK GROUP: | 2 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
IIIC |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| MyleA.18344.a.A1.GE34190 | full length | 1 | 731 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| MyleA.18344.a.A1.PW34800 | full length | 1 | 731 |
Structures
4EX4
DEPOSITED: 4/29/2012
DETERMINATION: XRay
CLONE: MyleA.18344.a.A1.GE34190
PROTEIN: MyleA.18344.a.A1.PW34800
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| OrthoMCL: | OG5_159543 |
| PATRIC ID: | fig|561304.4.peg.3918 |
| RefSeq: | YP_002504009.1 |
| UniProt: | B8ZSN3 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTDRVSAGNL RVARVLYDFV NNEALPGTDI NPNSFWSGVA KVVADLTPQN QSLLNSRDEL QAQIDKWHRH RVIEPFDVDA YRQFLIDIGY LLPEPDDFTI STSGVDDEIT MTAGPQLVVP VLNARFALNA ANARWGSLYD ALYGTDTIPE TEGAEKGSEY NKIRGDKVIA YARKFMDQAV PLASDSWTNA TGVSIFDGQL QIAIGTNSTG LASPEKFVGY NRQLRSSNWS VLLANHGLHI EVLIDPESPI GKTDPVGIKD VILESAITTI MDFEDSVTAV DADDKVRGYR NWLGLNKGDL TEEVNKDGKT FTRVLNADRS YTTPDGGELT LPGRSLLFVR NVGHLTTSDA ILVDGGDGQE KEVFEGIIDA VFTGLAAIHG LKTGEANGPL TNSRTGSIYI VKPKMHGPAE VAFTCELFSR VEDVLGLPQG TLKVGIMDEE RRTTLNLKAC IKAAADRVVF INTGFLDRTG DEIHTSMEAG PMIRKGAMKN STWIKAYEDA NVDIGLAAGF KGKAQIGKGM WAMTELMADM VEQKIGQPKA GATTAWVPSP TAATLHAMHY HQVDVAAVQQ ELTGQRRATV DQLLTIPLAK ELAWAPEEIR EEVDNDCQSI LGYVVRWVDQ GIGCSKVPDI HNVALMEDRA TLRISSQLLA NWLRHGVITS EDVRASLERM APLVDQQNAE DPAYRPMAPN FDDSIAFLAA QELILSGAQQ PNGYTEPILH RRRREFKAQN R
NT Sequence
atgactgatc gcgtgtcggc ggggaatttg cgcgtcgccc gtgtgcttta cgacttcgtc aacaacgaag ccctgcctgg caccgacatc aacccaaaca gcttctggtc gggcgtcgcc aaggtcgtcg ccgacctcac cccgcaaaac caaagtttgc tgaactcccg cgatgagctg caggcccaga ttgacaaatg gcaccgccat cgtgtgatcg agccattcga cgtggatgct tatcggcagt tcctcatcga tatcggctat ctgctgcctg aacccgatga cttcaccatc agcacctcag gagtagacga cgagatcacc atgaccgccg gcccgcagct ggtggtgccg gtacttaacg cacggttcgc actcaacgcc gccaacgccc gctggggctc cctttacgac gctctatatg gcaccgacac cataccggaa accgagggag ccgaaaaggg ctctgagtac aacaagattc gcggcgacaa ggtgatcgcc tacgcacgca agtttatgga tcaggccgtc ccgctggctt ctgactcatg gactaacgct acgggagtga gcatcttcga cggccagctt cagatcgcca tcggcacgaa ttccaccgga ttagccagtc ccgaaaagtt tgtcggctac aatcgccagt tgcgatcctc caactggtcg gtgctgttgg ccaatcacgg cctgcacatc gaggtcttga tcgacccgga atcaccaata ggcaagactg accccgtcgg catcaaggat gtgatcctgg aatcggccat caccacgatc atggacttcg aagactcagt cactgccgtc gacgccgacg acaaggtacg cggctaccgc aactggctcg gcctgaacaa aggtgactta accgaggaag tcaacaagga cggcaagacc ttcacccgcg tgctcaacgc cgaccgcagc tacaccacgc ccgacggcgg tgaattgacc ctgcccgggc gcagcctgtt gttcgtccgc aacgtgggcc atctgacgac cagcgacgcg attctcgtgg acggaggtga cggtcaggaa aaagaagtgt tcgagggcat catagatgca gtgttcaccg gtctggccgc aatccacgga ctcaaaaccg gcgaagctaa tggcccactg accaacagcc gcaccggttc catctacatc gtcaagccga aaatgcacgg tcccgcggag gttgcgttca cttgcgagct gttcagccgc gtcgaagacg tgctggggtt gccgcagggc accttgaagg tcggcatcat ggatgaggag cggcgtacga cactcaattt gaaggcgtgc atcaaggccg ccgccgatcg ggtcgtgttt atcaacactg gcttcttgga ccgcaccggc gacgagatcc acacctcaat ggaggccggc ccgatgattc gtaagggtgc gatgaaaaac agcacctgga tcaaagccta cgaggacgcc aatgttgaca tcggactagc cgcaggcttc aagggcaaag cacagatcgg caagggcatg tgggcaatga ccgaattaat ggctgatatg gtcgagcaga agatcggcca gcctaaggcg ggcgccacca ccgcgtgggt accctcgccc actgcggcca cgctgcatgc gatgcactac caccaggtcg acgtggctgc cgtgcaacaa gaactgaccg gccagagacg tgccaccgtt gaccagctgt tgaccattcc gttggccaag gaactggcct gggctcccga ggagatccgc gaggaggtcg ataatgactg tcagtccatc ctcggttatg tggtgcgctg ggtggaccag ggtatcggtt gctcgaaggt gcctgacatc cacaatgtgg cactaatgga ggaccgcgcc accctgcgga tctcgagcca gttgctagca aactggctgc gccacggcgt aatcaccagc gaggacgtgc gggccagcct ggaacggatg gcgccgttag tcgatcagca aaacgccgaa gacccagcat atcgcccgat ggcgcctaat ttcgatgaca gcatcgcgtt cctagcggcg caggaactga tactgtccgg cgcacaacag cccaacggct ataccgagcc gatcctgcat cgccgtcgtc gcgaattcaa ggcacagaac cggtga
Details for MyleA.18344.a.A1.GE34190
| HARVESTED ON: | 12/6/2011 |
| SEQUENCED ON: | 12/8/2011 |
| EXPECTED MW: | 82kDa |
| OBSERVED MW: | 82kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Many (50-100) |
| TOTAL EXPRESSION LEVEL: | High Expression |
| SOLUBLE EXPRESSION LEVEL | High Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass with incomplete coverage |
| PERCENT IDENTITY: | 78 |
| PERCENT COVERAGE: | 51 |
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMTDRVSAGN LRVARVLYDF VNNEALPGTD INPNSFWSGV AKVVADLTPQ NQSLLNSRDE LQAQIDKWHR HRVIEPFDVD AYRQFLIDIG YLLPEPDDFT ISTSGVDDEI TMTAGPQLVV PVLNARFALN AANARWGSLY DALYGTDTIP ETEGAEKGSE YNKIRGDKVI AYARKFMDQA VPLASDSWTN ATGVSIFDGQ LQIAIGTNST GXASPEKFVG YNRQLRSSNW SVLLANHGLH IEVLIDPESP IGKTDPVGIK DVILESAITT IMDFXTQSLP STXXQVRGYX XXRXXXXXLX XXXXXRXXPX XXXXSXXXPX AXXXXRXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXX
Validated NT Sequence
annnnnnttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gactgatcgc gtgtcggcgg ggaatttgcg cgtcgcccgt gtgctttacg acttcgtcaa caacgaagcc ctgcctggca ccgacatcaa cccaaacagc ttctggtcgg gcgtcgccaa ggtcgtcgcc gacctcaccc cgcaaaacca aagtttgctg aactcccgcg atgagctgca ggcccagatt gacaaatggc accgccatcg tgtgatcgag ccattcgacg tggatgctta tcggcagttc ctcatcgata tcggctatct gctgcctgaa cccgatgact tcaccatcag cacctcagga gtagacgacg agatcaccat gaccgccggc ccgcagctgg tggtgccggt acttaacgca cggttcgcac tcaacgccgc caacgcccgc tggggctccc tttacgacgc tctatatggc accgacacca taccggaaac cgagggagcc gaaaagggct ctgagtacaa caagattcgc ggcgacaagg tgatcgccta cgcacgcaag tttatggatc aggccgtccc gctggcttct gactcatgga ctaacgctac gggagtgagc atcttcgacg gccagcttca gatcgccatc ggcacgaatt ccaccggant agccagtccc gaaaagtttg tcggctacaa tcgccagttg cgatcctcca actggtcggt gctgttggcc aatcacggcc tgcacatcga ggtcttgatc gacccggaat caccaatagg caagactgac cccgtcggca tcaaggatgt gatcctggaa tcggccatca ccacgatcat ggacttcgan actcagtcac tgccgtcgac ncnacnacag gtacgcggct acnnantggn tcggnnganc naagnnnact tancnannan tnannnnngn agacnnnacc cgnnnntnnn ncnacncagc tnnnccncnc cnanggcgnn nannnnnnnc cggnnnnnnc nnntnnnnnn nnnnnnnnnn nnnnnnnnnn ncnnnnannn nnnnnnnnnn nngnngnnnn nnnnnnannn nnnnnnnnnn nnnannnnnn nnnnnnnnnn nnnnnnnnnn nnnna
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMTDRVSAGN LRVARVLYDF VNNEALPGTD INPNSFWSGV AKVVADLTPQ NQSLLNSRDE LQAQIDKWHR HRVIEPFDVD AYRQFLIDIG YLLPEPDDFT ISTSGVDDEI TMTAGPQLVV PVLNARFALN AANARWGSLY DALYGTDTIP ETEGAEKGSE YNKIRGDKVI AYARKFMDQA VPLASDSWTN ATGVSIFDGQ LQIAIGTNST GLASPEKFVG YNRQLRSSNW SVLLANHGLH IEVLIDPESP IGKTDPVGIK DVILESAITT IMDFEDSVTA VDADDKVRGY RNWLGLNKGD LTEEVNKDGK TFTRVLNADR SYTTPDGGEL TLPGRSLLFV RNVGHLTTSD AILVDGGDGQ EKEVFEGIID AVFTGLAAIH GLKTGEANGP LTNSRTGSIY IVKPKMHGPA EVAFTCELFS RVEDVLGLPQ GTLKVGIMDE ERRTTLNLKA CIKAAADRVV FINTGFLDRT GDEIHTSMEA GPMIRKGAMK NSTWIKAYED ANVDIGLAAG FKGKAQIGKG MWAMTELMAD MVEQKIGQPK AGATTAWVPS PTAATLHAMH YHQVDVAAVQ QELTGQRRAT VDQLLTIPLA KELAWAPEEI REEVDNDCQS ILGYVVRWVD QGIGCSKVPD IHNVALMEDR ATLRISSQLL ANWLRHGVIT SEDVRASLER MAPLVDQQNA EDPAYRPMAP NFDDSIAFLA AQELILSGAQ QPNGYTEPIL HRRRREFKAQ NR
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gactgatcgc gtgtcggcgg ggaatttgcg cgtcgcccgt gtgctttacg acttcgtcaa caacgaagcc ctgcctggca ccgacatcaa cccaaacagc ttctggtcgg gcgtcgccaa ggtcgtcgcc gacctcaccc cgcaaaacca aagtttgctg aactcccgcg atgagctgca ggcccagatt gacaaatggc accgccatcg tgtgatcgag ccattcgacg tggatgctta tcggcagttc ctcatcgata tcggctatct gctgcctgaa cccgatgact tcaccatcag cacctcagga gtagacgacg agatcaccat gaccgccggc ccgcagctgg tggtgccggt acttaacgca cggttcgcac tcaacgccgc caacgcccgc tggggctccc tttacgacgc tctatatggc accgacacca taccggaaac cgagggagcc gaaaagggct ctgagtacaa caagattcgc ggcgacaagg tgatcgccta cgcacgcaag tttatggatc aggccgtccc gctggcttct gactcatgga ctaacgctac gggagtgagc atcttcgacg gccagcttca gatcgccatc ggcacgaatt ccaccggatt agccagtccc gaaaagtttg tcggctacaa tcgccagttg cgatcctcca actggtcggt gctgttggcc aatcacggcc tgcacatcga ggtcttgatc gacccggaat caccaatagg caagactgac cccgtcggca tcaaggatgt gatcctggaa tcggccatca ccacgatcat ggacttcgaa gactcagtca ctgccgtcga cgccgacgac aaggtacgcg gctaccgcaa ctggctcggc ctgaacaaag gtgacttaac cgaggaagtc aacaaggacg gcaagacctt cacccgcgtg ctcaacgccg accgcagcta caccacgccc gacggcggtg aattgaccct gcccgggcgc agcctgttgt tcgtccgcaa cgtgggccat ctgacgacca gcgacgcgat tctcgtggac ggaggtgacg gtcaggaaaa agaagtgttc gagggcatca tagatgcagt gttcaccggt ctggccgcaa tccacggact caaaaccggc gaagctaatg gcccactgac caacagccgc accggttcca tctacatcgt caagccgaaa atgcacggtc ccgcggaggt tgcgttcact tgcgagctgt tcagccgcgt cgaagacgtg ctggggttgc cgcagggcac cttgaaggtc ggcatcatgg atgaggagcg gcgtacgaca ctcaatttga aggcgtgcat caaggccgcc gccgatcggg tcgtgtttat caacactggc ttcttggacc gcaccggcga cgagatccac acctcaatgg aggccggccc gatgattcgt aagggtgcga tgaaaaacag cacctggatc aaagcctacg aggacgccaa tgttgacatc ggactagccg caggcttcaa gggcaaagca cagatcggca agggcatgtg ggcaatgacc gaattaatgg ctgatatggt cgagcagaag atcggccagc ctaaggcggg cgccaccacc gcgtgggtac cctcgcccac tgcggccacg ctgcatgcga tgcactacca ccaggtcgac gtggctgccg tgcaacaaga actgaccggc cagagacgtg ccaccgttga ccagctgttg accattccgt tggccaagga actggcctgg gctcccgagg agatccgcga ggaggtcgat aatgactgtc agtccatcct cggttatgtg gtgcgctggg tggaccaggg tatcggttgc tcgaaggtgc ctgacatcca caatgtggca ctaatggagg accgcgccac cctgcggatc tcgagccagt tgctagcaaa ctggctgcgc cacggcgtaa tcaccagcga ggacgtgcgg gccagcctgg aacggatggc gccgttagtc gatcagcaaa acgccgaaga cccagcatat cgcccgatgg cgcctaattt cgatgacagc atcgcgttcc tagcggcgca ggaactgata ctgtccggcg cacaacagcc caacggctat accgagccga tcctgcatcg ccgtcgtcgc gaattcaagg cacagaaccg gtaaacagca cgaacaagtt ctgcagccaa gcttctcgag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaa
Details for MyleA.18344.a.A1.PW34800
| PURIFICATION DATe: | 1/24/2012 |
| CONCENTRATION: | 19.28mg/ml |
| OBSERVED MW: | 81kDa |
| EXPRESSION LEVEL: | High Expression |
| PROTEIN PURIFICATION BUFFER: | 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 3 |
| VIAL VOLUME: | 110µl |
| PERCENT IDENTITY: | 78 |
| PERCENT COVERAGE: | 51 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMTDRVSAGN LRVARVLYDF VNNEALPGTD INPNSFWSGV AKVVADLTPQ NQSLLNSRDE LQAQIDKWHR HRVIEPFDVD AYRQFLIDIG YLLPEPDDFT ISTSGVDDEI TMTAGPQLVV PVLNARFALN AANARWGSLY DALYGTDTIP ETEGAEKGSE YNKIRGDKVI AYARKFMDQA VPLASDSWTN ATGVSIFDGQ LQIAIGTNST GXASPEKFVG YNRQLRSSNW SVLLANHGLH IEVLIDPESP IGKTDPVGIK DVILESAITT IMDFXTQSLP STXXQVRGYX XXRXXXXXLX XXXXXRXXPX XXXXSXXXPX AXXXXRXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXX
Validated NT Sequence
annnnnnttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gactgatcgc gtgtcggcgg ggaatttgcg cgtcgcccgt gtgctttacg acttcgtcaa caacgaagcc ctgcctggca ccgacatcaa cccaaacagc ttctggtcgg gcgtcgccaa ggtcgtcgcc gacctcaccc cgcaaaacca aagtttgctg aactcccgcg atgagctgca ggcccagatt gacaaatggc accgccatcg tgtgatcgag ccattcgacg tggatgctta tcggcagttc ctcatcgata tcggctatct gctgcctgaa cccgatgact tcaccatcag cacctcagga gtagacgacg agatcaccat gaccgccggc ccgcagctgg tggtgccggt acttaacgca cggttcgcac tcaacgccgc caacgcccgc tggggctccc tttacgacgc tctatatggc accgacacca taccggaaac cgagggagcc gaaaagggct ctgagtacaa caagattcgc ggcgacaagg tgatcgccta cgcacgcaag tttatggatc aggccgtccc gctggcttct gactcatgga ctaacgctac gggagtgagc atcttcgacg gccagcttca gatcgccatc ggcacgaatt ccaccggant agccagtccc gaaaagtttg tcggctacaa tcgccagttg cgatcctcca actggtcggt gctgttggcc aatcacggcc tgcacatcga ggtcttgatc gacccggaat caccaatagg caagactgac cccgtcggca tcaaggatgt gatcctggaa tcggccatca ccacgatcat ggacttcgan actcagtcac tgccgtcgac ncnacnacag gtacgcggct acnnantggn tcggnnganc naagnnnact tancnannan tnannnnngn agacnnnacc cgnnnntnnn ncnacncagc tnnnccncnc cnanggcgnn nannnnnnnc cggnnnnnnc nnntnnnnnn nnnnnnnnnn nnnnnnnnnn ncnnnnannn nnnnnnnnnn nngnngnnnn nnnnnnannn nnnnnnnnnn nnnannnnnn nnnnnnnnnn nnnnnnnnnn nnnna
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMTDRVSAGN LRVARVLYDF VNNEALPGTD INPNSFWSGV AKVVADLTPQ NQSLLNSRDE LQAQIDKWHR HRVIEPFDVD AYRQFLIDIG YLLPEPDDFT ISTSGVDDEI TMTAGPQLVV PVLNARFALN AANARWGSLY DALYGTDTIP ETEGAEKGSE YNKIRGDKVI AYARKFMDQA VPLASDSWTN ATGVSIFDGQ LQIAIGTNST GLASPEKFVG YNRQLRSSNW SVLLANHGLH IEVLIDPESP IGKTDPVGIK DVILESAITT IMDFEDSVTA VDADDKVRGY RNWLGLNKGD LTEEVNKDGK TFTRVLNADR SYTTPDGGEL TLPGRSLLFV RNVGHLTTSD AILVDGGDGQ EKEVFEGIID AVFTGLAAIH GLKTGEANGP LTNSRTGSIY IVKPKMHGPA EVAFTCELFS RVEDVLGLPQ GTLKVGIMDE ERRTTLNLKA CIKAAADRVV FINTGFLDRT GDEIHTSMEA GPMIRKGAMK NSTWIKAYED ANVDIGLAAG FKGKAQIGKG MWAMTELMAD MVEQKIGQPK AGATTAWVPS PTAATLHAMH YHQVDVAAVQ QELTGQRRAT VDQLLTIPLA KELAWAPEEI REEVDNDCQS ILGYVVRWVD QGIGCSKVPD IHNVALMEDR ATLRISSQLL ANWLRHGVIT SEDVRASLER MAPLVDQQNA EDPAYRPMAP NFDDSIAFLA AQELILSGAQ QPNGYTEPIL HRRRREFKAQ NR
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GACTGATCGC GTGTCGGCGG GGAATTTGCG CGTCGCCCGT GTGCTTTACG ACTTCGTCAA CAACGAAGCC CTGCCTGGCA CCGACATCAA CCCAAACAGC TTCTGGTCGG GCGTCGCCAA GGTCGTCGCC GACCTCACCC CGCAAAACCA AAGTTTGCTG AACTCCCGCG ATGAGCTGCA GGCCCAGATT GACAAATGGC ACCGCCATCG TGTGATCGAG CCATTCGACG TGGATGCTTA TCGGCAGTTC CTCATCGATA TCGGCTATCT GCTGCCTGAA CCCGATGACT TCACCATCAG CACCTCAGGA GTAGACGACG AGATCACCAT GACCGCCGGC CCGCAGCTGG TGGTGCCGGT ACTTAACGCA CGGTTCGCAC TCAACGCCGC CAACGCCCGC TGGGGCTCCC TTTACGACGC TCTATATGGC ACCGACACCA TACCGGAAAC CGAGGGAGCC GAAAAGGGCT CTGAGTACAA CAAGATTCGC GGCGACAAGG TGATCGCCTA CGCACGCAAG TTTATGGATC AGGCCGTCCC GCTGGCTTCT GACTCATGGA CTAACGCTAC GGGAGTGAGC ATCTTCGACG GCCAGCTTCA GATCGCCATC GGCACGAATT CCACCGGATT AGCCAGTCCC GAAAAGTTTG TCGGCTACAA TCGCCAGTTG CGATCCTCCA ACTGGTCGGT GCTGTTGGCC AATCACGGCC TGCACATCGA GGTCTTGATC GACCCGGAAT CACCAATAGG CAAGACTGAC CCCGTCGGCA TCAAGGATGT GATCCTGGAA TCGGCCATCA CCACGATCAT GGACTTCGAA GACTCAGTCA CTGCCGTCGA CGCCGACGAC AAGGTACGCG GCTACCGCAA CTGGCTCGGC CTGAACAAAG GTGACTTAAC CGAGGAAGTC AACAAGGACG GCAAGACCTT CACCCGCGTG CTCAACGCCG ACCGCAGCTA CACCACGCCC GACGGCGGTG AATTGACCCT GCCCGGGCGC AGCCTGTTGT TCGTCCGCAA CGTGGGCCAT CTGACGACCA GCGACGCGAT TCTCGTGGAC GGAGGTGACG GTCAGGAAAA AGAAGTGTTC GAGGGCATCA TAGATGCAGT GTTCACCGGT CTGGCCGCAA TCCACGGACT CAAAACCGGC GAAGCTAATG GCCCACTGAC CAACAGCCGC ACCGGTTCCA TCTACATCGT CAAGCCGAAA ATGCACGGTC CCGCGGAGGT TGCGTTCACT TGCGAGCTGT TCAGCCGCGT CGAAGACGTG CTGGGGTTGC CGCAGGGCAC CTTGAAGGTC GGCATCATGG ATGAGGAGCG GCGTACGACA CTCAATTTGA AGGCGTGCAT CAAGGCCGCC GCCGATCGGG TCGTGTTTAT CAACACTGGC TTCTTGGACC GCACCGGCGA CGAGATCCAC ACCTCAATGG AGGCCGGCCC GATGATTCGT AAGGGTGCGA TGAAAAACAG CACCTGGATC AAAGCCTACG AGGACGCCAA TGTTGACATC GGACTAGCCG CAGGCTTCAA GGGCAAAGCA CAGATCGGCA AGGGCATGTG GGCAATGACC GAATTAATGG CTGATATGGT CGAGCAGAAG ATCGGCCAGC CTAAGGCGGG CGCCACCACC GCGTGGGTAC CCTCGCCCAC TGCGGCCACG CTGCATGCGA TGCACTACCA CCAGGTCGAC GTGGCTGCCG TGCAACAAGA ACTGACCGGC CAGAGACGTG CCACCGTTGA CCAGCTGTTG ACCATTCCGT TGGCCAAGGA ACTGGCCTGG GCTCCCGAGG AGATCCGCGA GGAGGTCGAT AATGACTGTC AGTCCATCCT CGGTTATGTG GTGCGCTGGG TGGACCAGGG TATCGGTTGC TCGAAGGTGC CTGACATCCA CAATGTGGCA CTAATGGAGG ACCGCGCCAC CCTGCGGATC TCGAGCCAGT TGCTAGCAAA CTGGCTGCGC CACGGCGTAA TCACCAGCGA GGACGTGCGG GCCAGCCTGG AACGGATGGC GCCGTTAGTC GATCAGCAAA ACGCCGAAGA CCCAGCATAT CGCCCGATGG CGCCTAATTT CGATGACAGC ATCGCGTTCC TAGCGGCGCA GGAACTGATA CTGTCCGGCG CACAACAGCC CAACGGCTAT ACCGAGCCGA TCCTGCATCG CCGTCGTCGC GAATTCAAGG CACAGAACCG GTAAACAGCA CGAACAAGTT CTGCAGCCAA GCTTCTCGAG GATCCGGCTG CTAACAAAGC CCGAAAGGAA GCTGAGTTGG CTGCTGCCAC CGCTGAGCAA TAACTAGCAT AACCCCTTGG GGCCTCTAAA CGGGTCTTGA GGGGTTTTTT GCTGAAAGGA GGAACTATAT CCGGATATCC ACAGGACGGG TGTGGTCGCC ATGATCGCGT AGTCGATAGT GGCTCCAAGT AGCGAAGCGA GCAGGACTGG GCGGCGGCCA AAGCGGTCGG ACAGTGCTCC GAGAACGGGT GCGCATAGAA ATTGCATCAA CGCATATAGC GCTAGCAGCA CGCCATAGTG ACTGGCGATG CTGTCGGAAT GGACGATATC CCGCAAGAGG CCCGGCAGTA CCGGCATAAC CAAGCCTATG CCTACAGCAT CCAGGGTGAC GGTGCCGAGG ATGACGATGA GCGCATTGTT AGATTTCATA CACGGTGCCT GACTGCGTTA GCAATTTAAC TGTGATAAAC TACCGCATTA AAGCTTATCG ATGATAAGCT GTCAAACATG AGAA