MyleA.18341.a

PROBABLE PURINE NUCLEOSIDE PHOSPHORYLASE DEOD (INOSINE PHOSPHORYLASE) (PNP)

CENTER ID: MyleA.18341.a
ORGANISM: Mycobacterium leprae Br4923
ASSOCIATED DISEASE: Hansen's disease, leprosy
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MyleA.18341.a.A1.GE34189 full length 1 268
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|561304.4.peg.1274
RefSeq: YP_002503193.1
UniProt: A0A0H3MUE7

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTYTLLDPDE LARRAAQVIG ERTGILKHDV AVVLGSGWSS AVAALGSSRA VFPQAELPGF ITPNAAGHTG ELLSVRIGAH RVLVLAGRIH PYEGHDLRHV VHPVRTACAA GARIIVLTNA AGGLRADMAV GQLVLISDHL NLTTRSPLVG THFVDLTNAY TTRLRKLASD TDPTLTEGVY AAQPGPHYET PAEIRMLRML GADLVGMSTV HETIAARAAG AEVLGVSLVT NLAAGITGKP LNHAEVLAAG TASANRIGSL LADIIARF
NT Sequence
gtgacttaca ccctgctcga tcccgacgaa ctcgctcggc gggccgccca ggttattggt gagcgcaccg gtatccttaa gcacgacgtc gcagtcgtcc tcggatcggg atggtcctcg gcggttgcag cgctcggctc atcgagagcc gtgttccccc aggccgagct gcccgggttc ataacgccca acgcagccgg gcataccggc gagttgttgt cggtgcgtat tggcgcgcat cgggtgttgg tgctggccgg tcgcatccat ccctacgagg ggcatgacct taggcacgtc gtccatccag tacgcacggc gtgcgcggcc ggtgcacgca tcatcgttct cactaatgcg gccggcggac tgcgtgcaga catggcggtc ggccaactgg tgctgattag tgaccacctg aacctgacga cacgttcgcc gctagtcggc acgcacttcg tcgacttaac caacgcgtac acaacgcggc tccgaaaact cgccagcgac accgacccga cactgaccga aggcgtgtac gcggcccagc ccggcccaca ctatgagact cccgcggaaa tccggatgct gcggatgctg ggtgctgacc tagtgggcat gtcaacggtg cacgagacca tcgcagcacg ggctgcgggc gctgaggtgt tgggcgtgtc actggtgaca aacctggcgg ccgggatcac cggcaagcca cttaaccatg ctgaggtgct tgccgcgggg actgcgtcag cgaaccggat cgggtccctg ctggccgaca tcatagcccg gttttga
Details for MyleA.18341.a.A1.GE34189
HARVESTED ON: 12/6/2011
SEQUENCED ON: 12/8/2011
EXPECTED MW: 30kDa
OBSERVED MW: 30kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 98
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMVTYTLLDP DELARRAAQV IGERTGILKH DVAVVLGSGW SSAVAALGSS RAVFPQAELP GFITPNAAGH TGELLSVRIG AHRVLVLAGR IHPYEGHDLR HVVHPVRTAC AAGARIIVLT NAAGGLRADM AVGQLVLISD HLNLTTRSPL VGTHFVDLTN AYTTRLRKLA SDTDPTLTEG VYAAQPGPHY ETPAEIRMLR MLGADLVGMS TVHETIAARA AGAXVLGVSL VTNLAAGITG KPLNHAEVLX AGTASANRIG SLLAXXIARF
Validated NT Sequence
annnnnnttt tntttanctt taagaaggag atataccatg gctcatcacc atcaccatca tatgggtacc ctggaagctc agacccaggg tcctggttcg atggtgactt acaccctgct cgatcccgac gaactcgctc ggcgggccgc ccaggttatt ggtgagcgca ccggtatcct taagcacgac gtcgcagtcg tcctcggatc gggatggtcc tcggcggttg cagcgctcgg ctcatcgaga gccgtgttcc cccaggccga gctgcccggg ttcataacgc ccaacgcagc cgggcatacc ggcgagttgt tgtcggtgcg tattggcgcg catcgggtgt tggtgctggc cggtcgcatc catccctacg aggggcatga ccttaggcac gtcgtccatc cagtacgcac ggcgtgcgcg gccggtgcac gcatcatcgt tctcactaat gcggccggcg gactgcgtgc agacatggcg gtcggccaac tggtgctgat tagtgaccac ctgaacctga cgacacgttc gccgctagtc ggcacgcact tcgtcgactt aaccaacgcg tacacaacgc ggctccgaaa actcgccagc gacaccgacc cgacactgac cgaaggcgtg tacgcggccc agcccggccc acactatgag actcccgcgg aaatccggat gctgcggatg ctgggtgctg acctagtggg catgtcaacg gtgcacgaga ccatcgcagc acgggctgcg ggcgctgang tgttgggcgt gtcactggtg acaaacctgg cggccgggat caccggcaag ccacttaacc atgctgaggt gcttgncgcg gggactgcgt cagcgaaccg gatcgggtcc ctgctggccg anntcatagc ccggttttaa taaacagcac gaacaagtnc tgcagccaag cttctcgagg atccngctgc taacaaagcc cgaaaggaag ctnanttggn tgctgcnnnn nnnaannnnn nnnnnnnnnn nnnnnnnnnn cnttnggnnn nnnnnnnnnn nnnnnnnnnn ncnnnnccnn nnnnnnnnnn nncnanngnn ncnnnnnngn nnnnnngnnn nnnncnnnnn nnnnnnnnnn ntnnnnnnnc nnnnnnnnnn ncnnnnnnnn gnngng
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVTYTLLDP DELARRAAQV IGERTGILKH DVAVVLGSGW SSAVAALGSS RAVFPQAELP GFITPNAAGH TGELLSVRIG AHRVLVLAGR IHPYEGHDLR HVVHPVRTAC AAGARIIVLT NAAGGLRADM AVGQLVLISD HLNLTTRSPL VGTHFVDLTN AYTTRLRKLA SDTDPTLTEG VYAAQPGPHY ETPAEIRMLR MLGADLVGMS TVHETIAARA AGAEVLGVSL VTNLAAGITG KPLNHAEVLA AGTASANRIG SLLADIIARF
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtgacttac accctgctcg atcccgacga actcgctcgg cgggccgccc aggttattgg tgagcgcacc ggtatcctta agcacgacgt cgcagtcgtc ctcggatcgg gatggtcctc ggcggttgca gcgctcggct catcgagagc cgtgttcccc caggccgagc tgcccgggtt cataacgccc aacgcagccg ggcataccgg cgagttgttg tcggtgcgta ttggcgcgca tcgggtgttg gtgctggccg gtcgcatcca tccctacgag gggcatgacc ttaggcacgt cgtccatcca gtacgcacgg cgtgcgcggc cggtgcacgc atcatcgttc tcactaatgc ggccggcgga ctgcgtgcag acatggcggt cggccaactg gtgctgatta gtgaccacct gaacctgacg acacgttcgc cgctagtcgg cacgcacttc gtcgacttaa ccaacgcgta cacaacgcgg ctccgaaaac tcgccagcga caccgacccg acactgaccg aaggcgtgta cgcggcccag cccggcccac actatgagac tcccgcggaa atccggatgc tgcggatgct gggtgctgac ctagtgggca tgtcaacggt gcacgagacc atcgcagcac gggctgcggg cgctgaggtg ttgggcgtgt cactggtgac aaacctggcg gccgggatca ccggcaagcc acttaaccat gctgaggtgc ttgccgcggg gactgcgtca gcgaaccgga tcgggtccct gctggccgac atcatagccc ggttttaaac agcacgaaca agttctgcag ccaagcttct cgaggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaa