SSGCID: Target Page

MyleA.18335.a

PROBABLE THYMIDYLATE KINASE TMK (dTMP KINASE) (THYMIDYLIC ACID KINASE) (TMPK)

CENTER ID:	MyleA.18335.a
ORGANISM:	Mycobacterium leprae Br4923
ASSOCIATED DISEASE:	Hansen's disease, leprosy
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MyleA.18335.a.A1.GE34187	full length		1	210

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|561304.4.peg.1393
RefSeq:	YP_002503227.1
UniProt:	A0A0H3MYS5

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MLIAIEGVDG AGKRTLSEEL RQAFEATGKS VATLAFPRYR QSVAADIAAE ALHGQHGDLA SSVYAMALLF AFDRAGAVED IEALDRNHDI VILDRYVASN AAYSAARLHE DCSGRAVAWV QRIEYQRLRL PSPDWQVLLA VSVELAGKRS RYRARTDPDR LRDSYERDDG LQQRTGAVYA GLAAADWGGR WLVVDADIDS GWLVATLMGC

NT Sequence

gtgttgatcg cgattgaagg tgttgacggc gctggcaagc gcactttgtc cgaggagtta cgccaggcgt ttgaggccac tgggaagtcg gtagcaacgt tggctttccc gcgttaccgg caatcagtag ccgccgacat agctgcagag gcgctgcacg gccagcacgg tgatctcgcg tcgtcagtgt atgcgatggc cctgctgttc gcgttcgacc gcgccggggc ggtcgaggac atcgaagcgt tggatcgcaa ccatgacata gtgattcttg atcgctacgt cgcgtccaat gccgcctaca gcgccgcgcg tttgcatgag gactgcagcg ggagagcggt ggcctgggta cagcgaatcg aataccagag attgcggctg ccctctcccg attggcaagt gttgctcgcg gtctctgtcg agcttgctgg gaagcgttcc cgctaccggg cccgcactga tcccgaccgg ctgcgtgaca gctacgaacg cgacgacggc cttcagcagc gcactggcgc ggtatacgct ggcctggctg ccgcagattg gggcgggcgg tggctagtcg tcgatgcgga tatcgattcg ggctggttgg tagcaacttt gatgggctgt tag

Details for MyleA.18335.a.A1.GE34187

HARVESTED ON:	12/6/2011
SEQUENCED ON:	12/13/2011
EXPECTED MW:	25kDa
OBSERVED MW:	25kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Many (50-100)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SMVLIAIEGV DGAGKRTLSE ELRQAFEATG KSVATLAFPR YRQSVAADIA AEALHGQHGD LASSVYAMAL LFAFDRAGAV EDIEALDRNH DIVILDRYVA SNAAYSAARL HEDCSGRAVA WVQRIEYQRL RLPSPDWQVL LAVSVELAGK RSRYRARTDP DRLRDSYERD DGLQQRTGAV YAGLAAADWG GRWLVVDADI DSGWLVATLM GC

Validated NT Sequence

attttgttta ctttaagaag gagatatacc atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatggtgt tgatcgcgat tgaaggtgtt gacggcgctg gcaagcgcac tttgtccgag gagttacgcc aggcgtttga ggccactggg aagtcggtag caacgttggc tttcccgcgt taccggcaat cagtagccgc cgacatagct gcagaggcgc tgcacggcca gcacggtgat ctcgcgtcgt cagtgtatgc gatggccctg ctgttcgcgt tcgaccgcgc cggggcggtc gaggacatcg aagcgttgga tcgcaaccat gacatagtga ttcttgatcg ctacgtcgcg tccaatgccg cctacagcgc cgcgcgtttg catgaggact gcagcgggag agcggtggcc tgggtacagc gaatcgaata ccagagattg cggctgccct ctcccgattg gcaagtgttg ctcgcggtct ctgtcgagct tgctgggaag cgttcccgct accgggcccg cactgatccc gaccggctgc gtgacagcta cgaacgcgac gacggccttc agcagcgcac tggcgcggta tacgctggcc tggctgccgc agattggggc gggcggtggc tagtcgtcga tgcggatatc gattcgggct ggttggtagc aactttgatg ggctgttaat aaacagcacg aacaagttct gcagccaagc ttctcgagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgcca

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtgttgatc gcgattgaag gtgttgacgg cgctggcaag cgcactttgt ccgaggagtt acgccaggcg tttgaggcca ctgggaagtc ggtagcaacg ttggctttcc cgcgttaccg gcaatcagta gccgccgaca tagctgcaga ggcgctgcac ggccagcacg gtgatctcgc gtcgtcagtg tatgcgatgg ccctgctgtt cgcgttcgac cgcgccgggg cggtcgagga catcgaagcg ttggatcgca accatgacat agtgattctt gatcgctacg tcgcgtccaa tgccgcctac agcgccgcgc gtttgcatga ggactgcagc gggagagcgg tggcctgggt acagcgaatc gaataccaga gattgcggct gccctctccc gattggcaag tgttgctcgc ggtctctgtc gagcttgctg ggaagcgttc ccgctaccgg gcccgcactg atcccgaccg gctgcgtgac agctacgaac gcgacgacgg ccttcagcag cgcactggcg cggtatacgc tggcctggct gccgcagatt ggggcgggcg gtggctagtc gtcgatgcgg atatcgattc gggctggttg gtagcaactt tgatgggctg ttaaacagca cgaacaagtt ctgcagccaa gcttctcgag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaa