SSGCID: Target Page

MyleA.18333.a

BETA-CARBONIC ANHYDRASE CANB

CENTER ID:	MyleA.18333.a
ORGANISM:	Mycobacterium leprae Br4923
ASSOCIATED DISEASE:	Hansen's disease, leprosy
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MyleA.18333.a.A1.GE34172	full length		1	213

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|561304.4.peg.3649
RefSeq:	YP_002503923.1
UniProt:	A0A0H3MRK1

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MFNTNPIAAW KALKEGNERF VAGQPAHPSQ SVEHRASLAD TQKPLAVIFG CADSRVAAEL VFDQGLGDMF VVRTVGHVID SAVLGSIEYA VTVLDVPLVV VLGHDSCGAV TAALAAINEG NLPSGYVRDV VERVAPSVLL GRRDGLTRVD EFEERHVHET VAQAMARSTA IAQRVAAGTL AIVGVTYHLA DGRAVLRTHI GNLGEEVQLS HCS

NT Sequence

atgtttaaca ccaaccccat agcagcttgg aaagcactca aagagggtaa cgagcgattt gtcgccggcc aaccggcaca tcccagccag agcgtcgagc atcgggccag cctggccgat actcagaaac ccctcgcggt gatattcggc tgcgcggaca gtcgagttgc cgccgagctc gtctttgacc agggcctggg cgacatgttc gtggtgcgca ccgtcggtca tgtcatcgac tcggcggtgc tcggctccat cgaatacgcg gtaacagtgc tcgacgtgcc acttgtcgtg gtccttgggc atgacagctg cggcgccgta acggctgctt tggcagccat caacgagggc aatttgccaa gtggttatgt ccgggatgtg gtggaacggg tagcgccgtc ggtcctgctg ggccgccgcg acggtttgac tcgtgtcgac gagtttgaag aacggcacgt tcatgagacg gtggcgcagg ccatggcccg ttccacggcc atcgcgcagc gggtagctgc gggcaccttg gcgatcgtcg gcgtgaccta tcacctcgct gacggacggg cggtgttgcg cactcacatc ggcaacctcg gtgaggaagt tcaactttca cactgctcct ag

Details for MyleA.18333.a.A1.GE34172

HARVESTED ON:	12/6/2011
SEQUENCED ON:	12/8/2011
EXPECTED MW:	24kDa
OBSERVED MW:	26kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Many (50-100)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SMFNTNPIAA WKALKEGNER FVAGQPAHPS QSVEHRASLA DTQKPLAVIF GCADSRVAAE LVFDQGLGDM FVVRTVGHVI DSAVLGSIEY AVTVLDVPLV VVLGHDSCGA VTAALAAINE GNLPSGYVRD VVERVAPSVL LGRRDGLTRV DEFEERHVHE TVAQAMARST AIAQRVAAGT LAIVGVTYHL ADGRAVLRTH IGNLGEEVQL SHCS

Validated NT Sequence

ttattaggag cagtgtgaaa gttgaacttc ctcaccgagg ttgccgatgt gagtgcgcaa caccgcccgt ccgtcagcga ggtgataggt cacgccgacg atcgccaagg tgcccgcagc tacccgctgc gcgatggccg tggaacgggc catggcctgc gccaccgtct catgaacgtg ccgttcttca aactcgtcga cacgagtcaa accgtcgcgg cggcccagca ggaccgacgg cgctacccgt tccaccacat cccggacata accacttggc aaattgccct cgttgatggc tgccaaagca gccgttacgg cgccgcagct gtcatgccca aggaccacga caagtggcac gtcgagcact gttaccgcgt attcgatgga gccgagcacc gccgagtcga tgacatgacc gacggtgcgc accacgaaca tgtcgcccag gccctggtca aagacgagct cggcggcaac tcgactgtcc gcgcagccga atatcaccgc gaggggtttc tgagtatcgg ccaggctggc ccgatgctcg acgctctggc tgggatgtgc cggttggccg gcgacaaatc gctcgttacc ctctttgagt gctttccaag ctgctatggg gttggtgtta aacatcgaac caggaccctg ggtctgagct tccagggtac ccatatgatg gtgatggtga tgagccat

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gtttaacacc aaccccatag cagcttggaa agcactcaaa gagggtaacg agcgatttgt cgccggccaa ccggcacatc ccagccagag cgtcgagcat cgggccagcc tggccgatac tcagaaaccc ctcgcggtga tattcggctg cgcggacagt cgagttgccg ccgagctcgt ctttgaccag ggcctgggcg acatgttcgt ggtgcgcacc gtcggtcatg tcatcgactc ggcggtgctc ggctccatcg aatacgcggt aacagtgctc gacgtgccac ttgtcgtggt ccttgggcat gacagctgcg gcgccgtaac ggctgctttg gcagccatca acgagggcaa tttgccaagt ggttatgtcc gggatgtggt ggaacgggta gcgccgtcgg tcctgctggg ccgccgcgac ggtttgactc gtgtcgacga gtttgaagaa cggcacgttc atgagacggt ggcgcaggcc atggcccgtt ccacggccat cgcgcagcgg gtagctgcgg gcaccttggc gatcgtcggc gtgacctatc acctcgctga cggacgggcg gtgttgcgca ctcacatcgg caacctcggt gaggaagttc aactttcaca ctgctcctaa acagcacgaa caagttctgc agccaagctt ctcgaggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa