SSGCID: Target Page

MyleA.18315.a

PROBABLE REPLICATIVE DNA HELICASE DNAB

CENTER ID:	MyleA.18315.a
ORGANISM:	Mycobacterium leprae Br4923
ASSOCIATED DISEASE:	Hansen's disease, leprosy
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MyleA.18315.a.A1.GE34183	full length		1	604

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|561304.4.peg.5193
RefSeq:	YP_002504339.1
UniProt:	A0A0H3MSN1

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MAVVDDLAHS GMDAVPPSED FGRQPPQDLA AEQSVLGGML LSKDAIADVL ERLRSGDFYR PAHQNVYDAI LDLYGRGEPA DAVTVAAELD RRGLLRRIGG APYLHTLIST VPTAANAGYY AGIVAEKALL RRLVEAGTRV VQYGYAGAEG ADVAEVVDRA QAEIYDVAEC RLSENYVPLE DLLQPTMDDL DAIASNGGIS RGAPTGFTEL DEVTNGLHPG QMIIVAARPG VGKALALDTP LPTPTGWTAM GDVAVGDELL AVDEAPTRVV AATEVMLGRP CYEIEFSDGT VIVADAQHQW PTSYGIRTSA QLRCGLDIIA AAGSTPRHAG RLTTAAFMAP VLCIDSVRRV RSVPVRCVEV DNAAHLYLAG RGMVPTHNST LGLDFMRSCS IKHRLASVIF SLEMSKSEIV MRLLSAEAKI KLADMRSGRM TDDDWTRLAR RMSEISEAPL YIDDSPNLTM MEIRAKARRL RQKTNLKLVV VDYLQLMTSG KRFESRQVEV SEFSRHLKLL AKELELPVVA ISQLNRSPEQ RTDKKPMLSD LRESGSLEQD ADVVILLHRP DAFDRDDPRG GEADLILAKH RNGPTKTVTV AHQLHLSRFT NMAR

NT Sequence

atggcggtcg tcgatgatct ggcgcactca ggtatggatg cagttccacc cagtgaagat tttggccgtc agccaccgca ggacctagcc gcggagcagt cggtgttggg cgggatgctg ctgagtaagg atgccattgc tgacgttctc gagcggctgc gttctggtga cttttatcgt ccagcacacc agaatgttta cgatgcgatc ctagatctgt acgggcgagg agagccggcc gatgcggtga cggtggccgc cgaattggac cgccgcggat tgttgcgcag gatcggtggt gcgccgtatc tgcacactct gatctcgacg gtgccgactg ccgccaatgc aggttactat gcgggcatcg tcgctgaaaa ggcgctgctg cgtcgactgg tggaggccgg cacacgggtg gtgcagtacg gttacgccgg cgctgagggt gccgacgtgg ccgaggtggt cgaccgcgcg caggcagaaa tctacgacgt agccgagtgc cggctttctg agaactacgt accgctcgaa gatctactgc agccgacgat ggatgacctc gatgccatcg cttccaatgg gggtatctcg cgtggcgcgc cgactggctt caccgaactc gatgaggtaa ccaacggttt gcatccggga cagatgatca tcgtggcggc tcgtccgggt gtgggcaagg cgctcgcgct ggacacgccg ctgcccacac cgactggttg gacggcgatg ggtgatgttg ccgtcggtga cgagttgctg gccgttgacg aagcaccgac gcgcgtggtg gccgcgaccg aggtcatgct gggccggccg tgctacgaga tcgagttctc cgacggtacc gtcattgtcg cagacgccca gcaccaatgg ccgacgagtt acggtatccg gacgtcggct cagttgcgtt gtgggctgga cattattgct gcggccgggt cgacaccgcg ccacgctggg cgactgacca cggctgcatt catggctccg gtgctgtgca tcgactcagt gcggcgggtg cgcagcgttc cggtgcggtg tgtcgaggtg gacaatgctg ctcatctgta tctggctggt cgagggatgg tgccaacgca caactcgacc cttgggctgg acttcatgcg gtcgtgttcg atcaagcatc gcctggcgag cgttatcttc tctctggaga tgagtaagtc cgagatcgtg atgcggttgc tttcggcaga ggcgaaaatc aagctcgccg atatgcgttc gggtcgaatg accgacgatg actggacacg gctggcgcgg cggatgagcg aaatcagtga ggcgccactg tatatcgacg attcaccgaa cctgaccatg atggagatcc gtgccaaggc gcgccggctg cggcaaaaga caaacctgaa gctagttgtg gtcgattacc tgcagttgat gacgtcaggc aagcggttcg agtcgcgaca ggtggaagtc tcggagttct cccgccatct taaattgtta gccaaagaac ttgagctccc ggtagtggcg atcagccagc tgaaccgtag tcccgagcag cgcaccgaca agaagccgat gttgtctgat ttgcgggagt cgggaagcct agagcaggat gccgacgtcg tgatcttgct gcaccgtccg gatgcctttg atcgcgacga tccgcgtggt ggcgaggcgg acttgattct cgccaagcat cgtaacggcc caaccaagac agtcactgtc gcgcatcaat tgcacttgtc gcgattcacc aacatggcaa ggtaa

Details for MyleA.18315.a.A1.GE34183

HARVESTED ON:	12/6/2011
SEQUENCED ON:	12/8/2011
EXPECTED MW:	68kDa
OBSERVED MW:	75kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Many (50-100)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	75
PERCENT COVERAGE:	64

Validated AA Sequence

Validated NT Sequence

ttgtttaact ttaagaagga ganataccat ggctcatcac catcaccatc atatgggtac cctggaagct cagacccagg gtcctggttc gatggcggtc gtcgatgatc tggcgcactc aggtatggat gcagttccac ccagtgaaga ttttggccgt cagccaccgc aggacctagc cgcggagcag tcggtgttgg gcgggatgct gctgagtaag gatgccattg ctgacgttct cgagcggctg cgttctggtg acttttatcg tccagcacac cagaatgttt acgatgcgat cctagatctg tacgggcgag gagagccggc cgatgcggtg acggtggccg ccgaattgga ccgccgcgga ttgttgcgca ggatcggtgg tgcgccgtat ctgcacactc tgatctcgac ggtgccgact gccgccaatg caggttacta tgcgggcatc gtcgctgaaa aggcgctgct gcgtcgactg gtggaggccg gcacacgggt ggtgcagtac ggttacgccg gcgctgaggg tgccgacgtg gccgaggtgg tcgaccgcgc gcaggcagaa atctacgacg tagccgagtg ccggctttct gagaactacg taccgctcga agatctactg cagccgacga tggatgacct cgatgccatc gcttccaatg ggggtatctc gcgtggcgcg ccgactggct tcaccgaact cgatgangta accaacggtt tgcatccggg acagatgatc atcgtggcgg ctcgtccggg tgtgggcaag gngctcgcgc tggacacgcc gctgcccaca ccgactggnt ggacggcnat gggtgatgtt gccgtcggtg acgagttgct ggccnttgac naancaccga cgcgcgtggn ggncgcnacc gangtcatgc tgggccggcc gtgctacnan nntcgagtnn tncganngta ccgtcattgt cncanangcc cnncancnnn gnnnannant tacggnatcc nnangtcgnn tctncntnnn gggnnggnnn nttnntgctg nnnnnggncg annccnnnnc ncgctggnnn actgacnnnn ctgcntncnt ggnnnngnnn nnnnnatcnn nnnnngnngg nnnnnnnnnn nncnnnnnnn nnnnnnnnnn ngnnnnancn nnncnnnnnn nnnnnnnnng nnnngnnnnn nannntnnnn nnnnnnnngn ntnnnnnnnn nnnnnnnnnn nnc

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMAVVDDLAH SGMDAVPPSE DFGRQPPQDL AAEQSVLGGM LLSKDAIADV LERLRSGDFY RPAHQNVYDA ILDLYGRGEP ADAVTVAAEL DRRGLLRRIG GAPYLHTLIS TVPTAANAGY YAGIVAEKAL LRRLVEAGTR VVQYGYAGAE GADVAEVVDR AQAEIYDVAE CRLSENYVPL EDLLQPTMDD LDAIASNGGI SRGAPTGFTE LDEVTNGLHP GQMIIVAARP GVGKALALDT PLPTPTGWTA MGDVAVGDEL LAVDEAPTRV VAATEVMLGR PCYEIEFSDG TVIVADAQHQ WPTSYGIRTS AQLRCGLDII AAAGSTPRHA GRLTTAAFMA PVLCIDSVRR VRSVPVRCVE VDNAAHLYLA GRGMVPTHNS TLGLDFMRSC SIKHRLASVI FSLEMSKSEI VMRLLSAEAK IKLADMRSGR MTDDDWTRLA RRMSEISEAP LYIDDSPNLT MMEIRAKARR LRQKTNLKLV VVDYLQLMTS GKRFESRQVE VSEFSRHLKL LAKELELPVV AISQLNRSPE QRTDKKPMLS DLRESGSLEQ DADVVILLHR PDAFDRDDPR GGEADLILAK HRNGPTKTVT VAHQLHLSRF TNMAR

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggcggtcgtc gatgatctgg cgcactcagg tatggatgca gttccaccca gtgaagattt tggccgtcag ccaccgcagg acctagccgc ggagcagtcg gtgttgggcg ggatgctgct gagtaaggat gccattgctg acgttctcga gcggctgcgt tctggtgact tttatcgtcc agcacaccag aatgtttacg atgcgatcct agatctgtac gggcgaggag agccggccga tgcggtgacg gtggccgccg aattggaccg ccgcggattg ttgcgcagga tcggtggtgc gccgtatctg cacactctga tctcgacggt gccgactgcc gccaatgcag gttactatgc gggcatcgtc gctgaaaagg cgctgctgcg tcgactggtg gaggccggca cacgggtggt gcagtacggt tacgccggcg ctgagggtgc cgacgtggcc gaggtggtcg accgcgcgca ggcagaaatc tacgacgtag ccgagtgccg gctttctgag aactacgtac cgctcgaaga tctactgcag ccgacgatgg atgacctcga tgccatcgct tccaatgggg gtatctcgcg tggcgcgccg actggcttca ccgaactcga tgaggtaacc aacggtttgc atccgggaca gatgatcatc gtggcggctc gtccgggtgt gggcaaggcg ctcgcgctgg acacgccgct gcccacaccg actggttgga cggcgatggg tgatgttgcc gtcggtgacg agttgctggc cgttgacgaa gcaccgacgc gcgtggtggc cgcgaccgag gtcatgctgg gccggccgtg ctacgagatc gagttctccg acggtaccgt cattgtcgca gacgcccagc accaatggcc gacgagttac ggtatccgga cgtcggctca gttgcgttgt gggctggaca ttattgctgc ggccgggtcg acaccgcgcc acgctgggcg actgaccacg gctgcattca tggctccggt gctgtgcatc gactcagtgc ggcgggtgcg cagcgttccg gtgcggtgtg tcgaggtgga caatgctgct catctgtatc tggctggtcg agggatggtg ccaacgcaca actcgaccct tgggctggac ttcatgcggt cgtgttcgat caagcatcgc ctggcgagcg ttatcttctc tctggagatg agtaagtccg agatcgtgat gcggttgctt tcggcagagg cgaaaatcaa gctcgccgat atgcgttcgg gtcgaatgac cgacgatgac tggacacggc tggcgcggcg gatgagcgaa atcagtgagg cgccactgta tatcgacgat tcaccgaacc tgaccatgat ggagatccgt gccaaggcgc gccggctgcg gcaaaagaca aacctgaagc tagttgtggt cgattacctg cagttgatga cgtcaggcaa gcggttcgag tcgcgacagg tggaagtctc ggagttctcc cgccatctta aattgttagc caaagaactt gagctcccgg tagtggcgat cagccagctg aaccgtagtc ccgagcagcg caccgacaag aagccgatgt tgtctgattt gcgggagtcg ggaagcctag agcaggatgc cgacgtcgtg atcttgctgc accgtccgga tgcctttgat cgcgacgatc cgcgtggtgg cgaggcggac ttgattctcg ccaagcatcg taacggccca accaagacag tcactgtcgc gcatcaattg cacttgtcgc gattcaccaa catggcaagg taaacagcac gaacaagttc tgcagccaag cttctcgagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaa