SSGCID: Target Page

MyleA.18288.a

3-PHOSPHOSHIKIMATE 1-CARBOXYVINYLTRANSFERASE AROA (5-ENOLPYRUVYLSHIKIMATE-3-PHOSPHATE SYNTHASE) (EPSP SYNTHASE) (EPSPS)

CENTER ID:	MyleA.18288.a
ORGANISM:	Mycobacterium leprae Br4923
ASSOCIATED DISEASE:	Hansen's disease, leprosy
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MyleA.18288.a.A1.GE34179	full length		1	430

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|561304.4.peg.1429
RefSeq:	YP_002503238.1
UniProt:	A0A0H3MQ85

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MSIESWTAPV ASTPVQANVT IPGSKSQTNR ALMLAALAAA QGQGTSTIGG ALRSRDTELM ISALRTLGLH VDEAGSVLTV NGRITPGEDA QVDCGLAGTV LRFVPPLAAL SANPVTFDGD EQARARPITP LLDALRGLGV PVDGVGLPFQ VQGSGSVAGG TVAIDASASS QFVSGLLLCA ASFTQGVTVQ HTGSPVPSAP HIAMTVMMLR QAGVQVDDSV GNRWQVRPGT VAARHWVVEP DLTNAVAFLA AAAVSGGTVR ITGWPKTSVQ PADNILNILF RLNVVVNQTD SFLEVQGSTV YDGFDVDLRD VGELTPSVAA LAALATPGSV SQLHGIAHLR GHETDRLAAL STEINRLGGD CQETSDGLII TATPLRPGVW RAYADHRMAM AGAIVGLRVS GVEVDDIGAT HKTLPQFPQL WANMLKRSTG

NT Sequence

gtgagcatcg agtcctggac ggcccccgtc gcgtcgacac cggtgcaggc gaacgtgaca attccgggct ctaaatcgca aaccaaccga gcactgatgc tggcagcgct agcggcagca caaggccaag gcacctcgac catcggcggt gcactgcgca gcagggatac cgaactaatg atttcagcgt tacggacact gggcctacac gtcgacgagg ccggctctgt cttaacggtc aacggccgaa ttacccccgg cgaagacgct caagtggact gcggtctggc cggcacagta ttgcgttttg tcccaccgct ggcggcgctg agcgccaacc cggtcacctt cgacggcgac gaacaagccc gggcccggcc gatcacaccg ctgctggatg cgctgcgtgg tctgggcgtc cccgtcgatg gcgtcggttt gcctttccag gtgcaaggca gcgggtcagt cgccggtggc accgtggcca ttgacgcgtc ggcgtcctca cagttcgtct ccggcctgtt gttgtgcgca gcgtcgttca cccaaggagt gaccgtgcag cacaccggtt cgccggtacc gtcagctccg catatagcga tgacggttat gatgctgcgg caagccggtg tccaggtcga tgactcggtt ggtaaccgtt ggcaggtgcg acccggtaca gttgcggccc ggcactgggt cgtcgagccc gatctcacga acgctgttgc gttcctggcg gcagcagcgg tcagcggcgg taccgtgcgc atcacgggct ggccaaaaac cagtgtacag cccgcagaca atattctgaa catcttgttc cgactgaatg tcgttgttaa tcaaactgat tcattcctcg aagtacaagg atcgacggtg tacgatggct tcgacgtcga ccttcgcgac gtcggtgagc tcacgccgtc ggtagcagcg ctagcggcac tggcaacccc aggatcggtg tcgcaactac acggcatcgc ccatctgcgc ggccacgaaa ccgaccgact cgccgcactg agcaccgaaa tcaaccgtct gggcggcgac tgtcaggaaa cgtccgacgg tttgattatc accgcgacac cgctgcggcc cggcgtctgg cgagcgtatg ctgatcacag gatggcaatg gccggcgcga tcgtcgggtt gcgggtctcc ggagtcgaag tcgacgacat cggtgctacc cacaagacgc taccccagtt cccacaactg tgggcgaaca tgctcaagag gtcgaccggt tga

Details for MyleA.18288.a.A1.GE34179

HARVESTED ON:	12/6/2011
SEQUENCED ON:	12/8/2011
EXPECTED MW:	46kDa
OBSERVED MW:	47kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Many (50-100)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	98
PERCENT COVERAGE:	65

Validated AA Sequence

Validated NT Sequence

tttgnnnnnt ttaagaagga gatataccat ggctcatcac catcaccatc atatgggtac cctggaagct cagacccagg gtcctggttc gatggtgagc atcgagtcct ggacggcccc cgtcgcgtcg acaccggtgc aggcgaacgt gacaattccg ggctctaaat cgcaaaccaa ccgagcactg atgctggcag cgctagcggc agcacaaggc caaggcacct cgaccatcgg cggtgcactg cgcagcaggg ataccgaact aatgatttca gcgttacgga cactgggcct acacgtcgac gaggccggct ctgtcttaac ggtcaacggc cgaattaccc ccggcgaaga cgctcaagtg gactgcggtc tggccggcac agtattgcgt tttgtcccac cgctggcggc gctgagcgcc aacccggtca ccttcgacgg cgacgaacaa gcccgggccc ggccgatcac accgctgctg gatgcgctgc gtggtctggg cgtccccgtc gatggcgtcg gtttgccttt ccaggtgcaa ggcagcgggt cagtcgccgg tggcaccgtg gccattgacg cgtcggcgtc ctcacagttc gtctccggcc tgttgttgtg cgcagcgtcg ttcacccaag gagtgaccgt gcagcacacc ggttcgccgg taccgtcagc tccgcatata gcgatgacgg ttatgatgct gcggcaagcc ggtgtccagg tcgatgactc ggttggtaac cgttggcagg tgcgacccgg tacagttgcg gcccggcact gggtcgtcga gcccgatctc acgaacgctg ttgcgttcct ggcggcagca gcggtcagcg gcggtaccgt gcgcatcacg ggctggncaa aaaccagtgt acagcccnca ganntantct gaacatcttg ttccgactga atgtcgtgtt aatcaaactg ntnnttcntc nantacagna tcgangngta cnanggcttc nacgtcnacn ncnnnacnnc gnnnnnctca cnnnnnnnag cannnctnnn nnnctngnnn cccnnnatng gnnnnnnnnc nnnnnnnnnn nncnatnnnn nngnnnnnan nnnnnantnn ncnnnnnnan ncnnannnnn nnnnnnnngn nnnnnnnnnn nnnncnnnnn ntnnntnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn cnnnnnnnt

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMVSIESWTA PVASTPVQAN VTIPGSKSQT NRALMLAALA AAQGQGTSTI GGALRSRDTE LMISALRTLG LHVDEAGSVL TVNGRITPGE DAQVDCGLAG TVLRFVPPLA ALSANPVTFD GDEQARARPI TPLLDALRGL GVPVDGVGLP FQVQGSGSVA GGTVAIDASA SSQFVSGLLL CAASFTQGVT VQHTGSPVPS APHIAMTVMM LRQAGVQVDD SVGNRWQVRP GTVAARHWVV EPDLTNAVAF LAAAAVSGGT VRITGWPKTS VQPADNILNI LFRLNVVVNQ TDSFLEVQGS TVYDGFDVDL RDVGELTPSV AALAALATPG SVSQLHGIAH LRGHETDRLA ALSTEINRLG GDCQETSDGL IITATPLRPG VWRAYADHRM AMAGAIVGLR VSGVEVDDIG ATHKTLPQFP QLWANMLKRS TG

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtgagcatc gagtcctgga cggcccccgt cgcgtcgaca ccggtgcagg cgaacgtgac aattccgggc tctaaatcgc aaaccaaccg agcactgatg ctggcagcgc tagcggcagc acaaggccaa ggcacctcga ccatcggcgg tgcactgcgc agcagggata ccgaactaat gatttcagcg ttacggacac tgggcctaca cgtcgacgag gccggctctg tcttaacggt caacggccga attacccccg gcgaagacgc tcaagtggac tgcggtctgg ccggcacagt attgcgtttt gtcccaccgc tggcggcgct gagcgccaac ccggtcacct tcgacggcga cgaacaagcc cgggcccggc cgatcacacc gctgctggat gcgctgcgtg gtctgggcgt ccccgtcgat ggcgtcggtt tgcctttcca ggtgcaaggc agcgggtcag tcgccggtgg caccgtggcc attgacgcgt cggcgtcctc acagttcgtc tccggcctgt tgttgtgcgc agcgtcgttc acccaaggag tgaccgtgca gcacaccggt tcgccggtac cgtcagctcc gcatatagcg atgacggtta tgatgctgcg gcaagccggt gtccaggtcg atgactcggt tggtaaccgt tggcaggtgc gacccggtac agttgcggcc cggcactggg tcgtcgagcc cgatctcacg aacgctgttg cgttcctggc ggcagcagcg gtcagcggcg gtaccgtgcg catcacgggc tggccaaaaa ccagtgtaca gcccgcagac aatattctga acatcttgtt ccgactgaat gtcgttgtta atcaaactga ttcattcctc gaagtacaag gatcgacggt gtacgatggc ttcgacgtcg accttcgcga cgtcggtgag ctcacgccgt cggtagcagc gctagcggca ctggcaaccc caggatcggt gtcgcaacta cacggcatcg cccatctgcg cggccacgaa accgaccgac tcgccgcact gagcaccgaa atcaaccgtc tgggcggcga ctgtcaggaa acgtccgacg gtttgattat caccgcgaca ccgctgcggc ccggcgtctg gcgagcgtat gctgatcaca ggatggcaat ggccggcgcg atcgtcgggt tgcgggtctc cggagtcgaa gtcgacgaca tcggtgctac ccacaagacg ctaccccagt tcccacaact gtgggcgaac atgctcaaga ggtcgaccgg ttaaacagca cgaacaagtt ctgcagccaa gcttctcgag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaa