MyleA.18279.a

ADENYLYL CYCLASE (ATP PYROPHOSPHATE-LYASE) (ADENYLATE CYCLASE)

CENTER ID: MyleA.18279.a
ORGANISM: Mycobacterium leprae Br4923
ASSOCIATED DISEASE: Hansen's disease, leprosy
CURRENT STATUS: soluble
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MyleA.18279.a.A1.GE34178 full length 1 324
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|561304.4.peg.2607
RefSeq: YP_002503626.1
UniProt: A0A0H3MVB1

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MPPSAKSTYP GQVEGAPHDG TPSAPQEPDE DTVTVPAPAL IRRSSVSMPN AAQWLHTTNR SPRLVAMVRR ARRLLPGDPD FGDPLSTAGE GGPRAAARAA DRLLGDRGAA SREVSLSVLQ VWQALTEAIA RRPVNPEVTL VFTDLVGFSG WSLQAGDEAT LALLRQVARA VESPLLDAGG HIVKRMGDGI MAVFRDPSVA VQAVLAATEA MKSVEVGGYT PRIRVGIHTG RPQRLAADWL GVDVNIAARV MERATKGGIM ISGPTLDLIP QSDLKELGII TRRVRKPMFT SKFTGIPPDM VIYRIKARRE LTASDETAQT NSLT
NT Sequence
ttgcccccgt ccgcaaaatc tacctaccct ggccaagtgg aaggcgctcc tcacgatggg accccgtccg cgcctcagga accggacgag gacacggtca ccgtgccggc gccggccttg atacgacgtt cttcggtgtc gatgcccaac gccgcacagt ggctacatac cacaaaccgc agcccccggc tagtggcgat ggtccggcgg gcacgacgac tgttgcctgg cgatcctgac tttggtgatc ctctgtccac cgcgggtgag ggtggcccac gtgccgcggc acgagctgcc gaccggttgc taggggatcg cggtgcggcg tcgcgtgagg tgagtctgag tgtgctacag gtgtggcagg cattgaccga ggccattgcc cgacggccgg tcaatccgga ggtgacgctg gtattcaccg acctggttgg cttctccggg tggtcattgc aagctggtga cgaagcgacc ctggcactgc tgcgacaggt agcgcgcgct gttgagtcac cgttgctcga tgccggtggg catatcgtca agcgaatggg ggacgggatc atggcagtgt ttcgcgatcc gtcagtcgcc gtacaggccg tactggctgc cacggaagcg atgaaatcag tcgaggtagg aggctacaca ccgcgaatcc gtgtcggaat acacactggc cggccacaac gactggccgc ggactggctt ggtgttgacg tcaatatcgc cgcgcgcgtt atggagcgcg cgacaaaagg tggcatcatg atctctggcc ctaccctgga cctgatacca caaagtgacc tgaaagagct aggcattatt actaggcgcg tccgaaaacc catgttcacc agcaaattca ccggtattcc cccggacatg gtgatttatc gcatcaaggc tcgtagggag ttgacagctt cagatgaaac agctcaaaca aattccctga catag
Details for MyleA.18279.a.A1.GE34178
HARVESTED ON: 12/6/2011
SEQUENCED ON: 10/23/2013
EXPECTED MW: 37kDa
OBSERVED MW: 40kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMLPPSAKST YPGQVEGAPH DGTPSAPQEP DEDTVTVPAP ALIRRSSVSM PNAAQWLHTT NRSPRLVAMV RRARRLLPGD PDFGDPLSTA GEGGPRAAAR AADRLLGDRG AASREVSLSV LQVWQALTEA IARRPVNPEV TLVFTDLVGF SGWSLQAGDE ATLALLRQVA RAVESPLLDA GGHIVKRMGD GIMAVFRDPS VAVQAVLAAT EAMKSVEVGG YTPRIRVGIH TGRPQRLAAD WLGVDVNIAA RVMERATKGG IMISGPTLDL IPQSDLKELG IITRRVRKPM FTSKFTGIPP DMVIYRIKAR RELTASDETA QTNSLT
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgttgc ccccgtccgc aaaatctacc taccctggcc aagtggaagg cgctcctcac gatgggaccc cgtccgcgcc tcaggaaccg gacgaggaca cggtcaccgt gccggcgccg gccttgatac gacgttcttc ggtgtcgatg cccaacgccg cacagtggct acataccaca aaccgcagcc cccggctagt ggcgatggtc cggcgggcac gacgactgtt gcctggcgat cctgactttg gtgatcctct gtccaccgcg ggtgagggtg gcccacgtgc cgcggcacga gctgccgacc ggttgctagg ggatcgcggt gcggcgtcgc gtgaggtgag tctgagtgtg ctacaggtgt ggcaggcatt gaccgaggcc attgcccgac ggccggtcaa tccggaggtg acgctggtat tcaccgacct ggttggcttc tccgggtggt cattgcaagc tggtgacgaa gcgaccctgg cactgctgcg acaggtagcg cgcgctgttg agtcaccgtt gctcgatgcc ggtgggcata tcgtcaagcg aatgggggac gggatcatgg cagtgtttcg cgatccgtca gtcgccgtac aggccgtact ggctgccacg gaagcgatga aatcagtcga ggtaggaggc tacacaccgc gaatccgtgt cggaatacac actggccggc cacaacgact ggccgcggac tggcttggtg ttgacgtcaa tatcgccgcg cgcgttatgg agcgcgcgac aaaaggtggc atcatgatct ctggccctac cctggacctg ataccacaaa gtgacctgaa agagctaggc attattacta ggcgcgtccg aaaacccatg ttcaccagca aattcaccgg tattcccccg gacatggtga tttatcgcat caaggctcgt agggagttga cagcttcaga tgaaacagct caaacaaatt ccctgacata ataa
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMLPPSAKST YPGQVEGAPH DGTPSAPQEP DEDTVTVPAP ALIRRSSVSM PNAAQWLHTT NRSPRLVAMV RRARRLLPGD PDFGDPLSTA GEGGPRAAAR AADRLLGDRG AASREVSLSV LQVWQALTEA IARRPVNPEV TLVFTDLVGF SGWSLQAGDE ATLALLRQVA RAVESPLLDA GGHIVKRMGD GIMAVFRDPS VAVQAVLAAT EAMKSVEVGG YTPRIRVGIH TGRPQRLAAD WLGVDVNIAA RVMERATKGG IMISGPTLDL IPQSDLKELG IITRRVRKPM FTSKFTGIPP DMVIYRIKAR RELTASDETA QTNSLT
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gttgcccccg tccgcaaaat ctacctaccc tggccaagtg gaaggcgctc ctcacgatgg gaccccgtcc gcgcctcagg aaccggacga ggacacggtc accgtgccgg cgccggcctt gatacgacgt tcttcggtgt cgatgcccaa cgccgcacag tggctacata ccacaaaccg cagcccccgg ctagtggcga tggtccggcg ggcacgacga ctgttgcctg gcgatcctga ctttggtgat cctctgtcca ccgcgggtga gggtggccca cgtgccgcgg cacgagctgc cgaccggttg ctaggggatc gcggtgcggc gtcgcgtgag gtgagtctga gtgtgctaca ggtgtggcag gcattgaccg aggccattgc ccgacggccg gtcaatccgg aggtgacgct ggtattcacc gacctggttg gcttctccgg gtggtcattg caagctggtg acgaagcgac cctggcactg ctgcgacagg tagcgcgcgc tgttgagtca ccgttgctcg atgccggtgg gcatatcgtc aagcgaatgg gggacgggat catggcagtg tttcgcgatc cgtcagtcgc cgtacaggcc gtactggctg ccacggaagc gatgaaatca gtcgaggtag gaggctacac accgcgaatc cgtgtcggaa tacacactgg ccggccacaa cgactggccg cggactggct tggtgttgac gtcaatatcg ccgcgcgcgt tatggagcgc gcgacaaaag gtggcatcat gatctctggc cctaccctgg acctgatacc acaaagtgac ctgaaagagc taggcattat tactaggcgc gtccgaaaac ccatgttcac cagcaaattc accggtattc ccccggacat ggtgatttat cgcatcaagg ctcgtaggga gttgacagct tcagatgaaa cagctcaaac aaattccctg acataaacag cacgaacaag ttctgcagcc aagcttctcg aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaa