MyleA.18274.a

Probable anthranilate phosphoribosyltransferase TrpD

CENTER ID: MyleA.18274.a
ORGANISM: Mycobacterium leprae Br4923
ASSOCIATED DISEASE: Hansen's disease, leprosy
CURRENT STATUS: soluble
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MyleA.18274.a.A1.GE34176 full length 1 366
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|561304.4.peg.1618
RefSeq: YP_002503299.1
UniProt: B8ZQM2

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MVLSSQPPSS RSATGSVLSW PQVLGRLTAG QDLTRGQAAW AMGQVMTGNA RPAQIAAFAV AMKMKVPTAA EVSELADVML SHARKLPTEA ICQDIGETVD IAGTGGDGAN TVNLSTMAAI VVAAAGVPVV KHGNRASSSL SGGADTLEAL GVRIDLGPEQ VANSLAEIGI GFCFAPLFQP SYRYASAVRH EIGVPTVFNI LGPLTNPAWP RAGLIGCAFG NLAEVMAGVF AARRSSVLVV HGDDGLDELT TTTTSTIWRV QAGTVDKLTF DPVEFGFARA HLDDLLGGDA QTNAAKVRAV LAGAKGPVRD AVVLNAAGAI VAYAGLSSHA EWSPAWDDGL ARASAAIDSG AAEQLLARWV RFSQQV
NT Sequence
gtggttttgt catcgcaacc accttcttct cgcagcgcca ccggatcggt gttgtcgtgg ccgcaggtct tggggcgttt gacagcaggg caggatctca cgcggggcca ggctgcttgg gcgatgggcc aggtcatgac tgggaacgca cggccagccc agatcgccgc gttcgcggtg gcgatgaaga tgaaggtccc caccgcggcc gaagtcagtg agcttgctga cgtcatgctc agccacgcgc gtaagctgcc gacagaggcg atctgccaag atatcggaga aaccgtagac attgccggaa ccgggggaga tggtgccaac acagtcaacc tgtccactat ggctgcgatc gtggttgcgg ccgcaggtgt accggtggtc aaacatggta accgggcctc gtcttcactg tcaggcggcg ctgacactct ggaagcattg ggggtccgta tcgaccttgg accagagcaa gtcgcgaata gcctagccga aatcgggatc gggttctgct tcgcgccgct gttccaaccg tcgtaccggt acgcgtccgc ggtgcgccat gagatcgggg tgccgaccgt gttcaacatc ctcgggccgc tgaccaatcc ggcctggccg cgggctggat tgatcggctg tgcgtttggc aatcttgccg aggttatggc gggggtgttc gctgcgcgac gctccagtgt gctggtagtg catggcgatg atggactcga cgaattgacc accactacta ccagtacgat ctggcgtgtt caggcgggca cagtggataa gctgactttt gatcctgtcg aattcgggtt tgcgcgtgct caccttgacg atctgttggg aggggatgcg caaactaatg cggcgaaggt gcgtgcggta ctggccgggg ccaagggccc agtccgcgac gccgttgtgc tcaacgccgc cggtgcgatc gtcgcctacg ctgggttatc cagccacgct gaatggtcgc ccgcgtggga cgatgggttg gcgcgcgcca gcgctgccat cgattcgggt gccgccgaac agctcctcgc gcggtgggtg aggtttagtc agcaggtctg a
Details for MyleA.18274.a.A1.GE34176
HARVESTED ON: 12/6/2011
SEQUENCED ON: 12/8/2011
EXPECTED MW: 40kDa
OBSERVED MW: 41kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 100
PERCENT COVERAGE: 92
Validated AA Sequence
MVVLSSQPPS SRSATGSVLS WPQVLGRLTA GQDLTRGQAA WAMGQVMTGN ARPAQIAAFA VAMKMKVPTA AEVSELADVM LSHARKLPTE AICQDIGETV DIAGTGGDGA NTVNLSTMAA IVVAAAGVPV VKHGNRASSS LSGGADTLEA LGVRIDLGPE QVANSLAEIG IGFCFAPLFQ PSYRYASAVR HEIGVPTVFN ILGPLTNPAW PRAGLIGCAF GNLAEVMAGV FAARRSSVLV VHGDDGLDEL TTTTTSTIWR VQAGTVDKLT FDPVEFGFAR AHLDDLLGGD AQTNAAKVRA VLAGAKGPVR DAVVLNAAGA IVAYAGLSSH AEWSPAWDDG LARASAAIDS GAAEQLLARW
Validated NT Sequence
acccaccgcg cgaggagctg ttcggcggca cccgaatcga tggcagcgct ggcgcgcgcc aacccatcgt cccacgcggg cgaccattca gcgtggctgg ataacccagc gtaggcgacg atcgcaccgg cggcgttgag cacaacggcg tcgcggactg ggcccttggc cccggccagt accgcacgca ccttcgccgc attagtttgc gcatcccctc ccaacagatc gtcaaggtga gcacgcgcaa acccgaattc gacaggatca aaagtcagct tatccactgt gcccgcctga acacgccaga tcgtactggt agtagtggtg gtcaattcgt cgagtccatc atcgccatgc actaccagca cactggagcg tcgcgcagcg aacacccccg ccataacctc ggcaagattg ccaaacgcac agccgatcaa tccagcccgc ggccaggccg gattggtcag cggcccgagg atgttgaaca cggtcggcac cccgatctca tggcgcaccg cggacgcgta ccggtacgac ggttggaaca gcggcgcgaa gcagaacccg atcccgattt cggctaggct attcgcgact tgctctggtc caaggtcgat acggaccccc aatgcttcca gagtgtcagc gccgcctgac agtgaagacg aggcccggtt accatgtttg accaccggta cacctgcggc cgcaaccacg atcgcagcca tagtggacag gttgactgtg ttggcaccat ctcccccggt tccggcaatg tctacggttt ctccgatatc ttggcagatc gcctctgtcg gcagcttacg cgcgtggctg agcatgacgt cagcaagctc actgacttcg gccgcggtgg ggaccttcat cttcatcgcc accgcgaacg cggcgatctg ggctggccgt gcgttcccag tcatgacctg gcccatcgcc caagcagcct ggccccgcgt gagatcctgc cctgctgtca aacgccccaa gacctgcggc cacgacaaca ccgatccggt ggcgctgcga gaagaaggtg gttgcgatga caaaaccacc atcgaaccag gaccctgggt ctgagcttcc aggg
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVVLSSQPP SSRSATGSVL SWPQVLGRLT AGQDLTRGQA AWAMGQVMTG NARPAQIAAF AVAMKMKVPT AAEVSELADV MLSHARKLPT EAICQDIGET VDIAGTGGDG ANTVNLSTMA AIVVAAAGVP VVKHGNRASS SLSGGADTLE ALGVRIDLGP EQVANSLAEI GIGFCFAPLF QPSYRYASAV RHEIGVPTVF NILGPLTNPA WPRAGLIGCA FGNLAEVMAG VFAARRSSVL VVHGDDGLDE LTTTTTSTIW RVQAGTVDKL TFDPVEFGFA RAHLDDLLGG DAQTNAAKVR AVLAGAKGPV RDAVVLNAAG AIVAYAGLSS HAEWSPAWDD GLARASAAID SGAAEQLLAR WVRFSQQV
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtggttttg tcatcgcaac caccttcttc tcgcagcgcc accggatcgg tgttgtcgtg gccgcaggtc ttggggcgtt tgacagcagg gcaggatctc acgcggggcc aggctgcttg ggcgatgggc caggtcatga ctgggaacgc acggccagcc cagatcgccg cgttcgcggt ggcgatgaag atgaaggtcc ccaccgcggc cgaagtcagt gagcttgctg acgtcatgct cagccacgcg cgtaagctgc cgacagaggc gatctgccaa gatatcggag aaaccgtaga cattgccgga accgggggag atggtgccaa cacagtcaac ctgtccacta tggctgcgat cgtggttgcg gccgcaggtg taccggtggt caaacatggt aaccgggcct cgtcttcact gtcaggcggc gctgacactc tggaagcatt gggggtccgt atcgaccttg gaccagagca agtcgcgaat agcctagccg aaatcgggat cgggttctgc ttcgcgccgc tgttccaacc gtcgtaccgg tacgcgtccg cggtgcgcca tgagatcggg gtgccgaccg tgttcaacat cctcgggccg ctgaccaatc cggcctggcc gcgggctgga ttgatcggct gtgcgtttgg caatcttgcc gaggttatgg cgggggtgtt cgctgcgcga cgctccagtg tgctggtagt gcatggcgat gatggactcg acgaattgac caccactact accagtacga tctggcgtgt tcaggcgggc acagtggata agctgacttt tgatcctgtc gaattcgggt ttgcgcgtgc tcaccttgac gatctgttgg gaggggatgc gcaaactaat gcggcgaagg tgcgtgcggt actggccggg gccaagggcc cagtccgcga cgccgttgtg ctcaacgccg ccggtgcgat cgtcgcctac gctgggttat ccagccacgc tgaatggtcg cccgcgtggg acgatgggtt ggcgcgcgcc agcgctgcca tcgattcggg tgccgccgaa cagctcctcg cgcggtgggt gaggtttagt cagcaggtct aaacagcacg aacaagttct gcagccaagc ttctcgagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aa